Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,903,6920CA40.8% 275.9 / 88.0 157intergenic (+318/+1055)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base C (6/87);  new base A (64/0);  total (70/87)
Fisher's exact test for biased strand distribution p-value = 1.64e-37
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

Only 100 of 163 total aligned reads displayed.

TTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGA  >  NC_000913/2903543‑2903842
                                                                                                                                                      |                                                                                                                                                          
 ttttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATTCGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAAc                                                                                                                                                            <  2:39532/149‑1 (MQ=255)
 ttttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAAcc                                                                                                                                                           <  1:1118398/150‑1 (MQ=255)
  tttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACAATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCa                                                                                                                                                          <  1:2024617/150‑1 (MQ=255)
   ttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCAc                                                                                                                                                         <  2:836777/150‑1 (MQ=255)
   ttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCAc                                                                                                                                                         <  2:388877/150‑1 (MQ=255)
    tACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACt                                                                                                                                                        <  2:1893171/150‑1 (MQ=255)
     aCAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TTTAAAAACa                                                                                                                                                          >  1:599739/1‑147 (MQ=255)
      cAAGGAATATTAACTACTCTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑At                                                                                                                                                    <  2:1510539/151‑1 (MQ=255)
      cAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAAACACCC‑a                                                                                                                                                     >  1:1201564/1‑150 (MQ=255)
       aaGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAAAcacac‑a                                                                                                                                                     >  1:1836110/1‑149 (MQ=255)
        aGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑a                                                                                                                                                 <  1:961606/151‑1 (MQ=255)
             tatTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TTTAAAAACAATA‑ATT‑atac                                                                                                                                              >  2:1371271/1‑146 (MQ=255)
              atTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAAGTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCa                                                                                                                                           <  2:483510/151‑1 (MQ=255)
               ttAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑ATTAAAACCaaacctttaacata                                                                                                                                             >  1:1952367/1‑137 (MQ=255)
               ttAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TTTAAAAAAACCC‑ATT‑TACCTc                                                                                                                                            >  1:2051280/1‑149 (MQ=255)
               ttAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAAACACAC‑ACT‑AGCCTcc                                                                                                                                           >  1:1300404/1‑149 (MQ=255)
                tAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCaa                                                                                                                                          <  1:1002011/150‑1 (MQ=255)
                 aaCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTTAAAAAACTC‑ATT‑TACCTCaaa                                                                                                                                         >  1:316549/1‑150 (MQ=255)
                 aaCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCaaa                                                                                                                                         <  1:1485884/150‑1 (MQ=255)
                  aCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAAc                                                                                                                                       <  1:1867855/151‑1 (MQ=255)
                     acacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACaaa                                                                                                                                    <  1:1763949/151‑1 (MQ=255)
                     acacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACa                                                                                                                                      <  1:680127/149‑1 (MQ=255)
                      cacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAg                                                                                                                                   <  1:1593399/151‑1 (MQ=255)
                      cacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAg                                                                                                                                   <  1:1452467/151‑1 (MQ=255)
                          cGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TTTAAAAAAACTC‑ATT‑ATCCTCAAAACaaaaa                                                                                                                                  >  2:89210/1‑146 (MQ=255)
                          cGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TTTAAAAAAAATC‑ATT‑ATCCTCCAaaaaaaaaaa                                                                                                                                >  2:268302/1‑146 (MQ=255)
                          cGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TTTAAAAAAACTC‑CTT‑ATCCTCAAAACaaaaa                                                                                                                                  >  2:1383771/1‑146 (MQ=255)
                          cGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACaa                                                                                                                               <  2:676973/151‑1 (MQ=255)
                            aTGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAAc                                                                                                                             <  2:963537/151‑1 (MQ=255)
                                 tttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAAATGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TTTAGAAAACCTC‑AAC‑TGCCTCaaaaaaaaaaaaaaa                                                                                                                             >  1:1536246/1‑145 (MQ=255)
                                   tAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAGCAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAGCTTcc                                                                                                                         <  1:1662119/148‑1 (MQ=255)
                                    aGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑At                                                                                                                    <  1:137126/151‑1 (MQ=255)
                                    aGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑At                                                                                                                    <  2:2044618/151‑1 (MQ=255)
                                      aaTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TTTAAAAACACTC‑ATT‑TTCCTCAAAAAAAAAACAAAATTCCAG‑Attt                                                                                                                  >  2:717884/1‑151 (MQ=255)
                                         aaaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATtta‑t                                                                                                               <  1:547544/150‑1 (MQ=255)
                                           aaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCATATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTAc                                                                                                            <  2:1902892/151‑1 (MQ=255)
                                                 aTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAAcc                                                                                                      <  1:1861001/151‑1 (MQ=255)
                                                  tACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TTTAAAAAAACTC‑CTT‑AACCTCAAAAAAAAAAAAAAAATTCCC‑ATTTA‑TTACtt                                                                                                          >  2:1229124/1‑146 (MQ=255)
                                                     gAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATg                                                                                                  <  1:303538/151‑1 (MQ=255)
                                                       aTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TTTAAAAACACTC‑ATT‑AGCCCCAAAACAAAAAAAAAAATCCAG‑AATTT‑TTTCTTTAACAAAGa                                                                                                 >  1:761546/1‑150 (MQ=255)
                                                       aTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TTTAAAAACACTC‑ATT‑AACCTCCAAAAAAAAACAAAAATCCAG‑Aatta‑tttttttaacaaaga                                                                                                 >  2:2191517/1‑137 (MQ=255)
                                                       aTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGaa                                                                                                <  1:1838738/151‑1 (MQ=255)
                                                         cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAAt                                                                                               <  1:1733382/150‑1 (MQ=255)
                                                         cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATa                                                                                              <  2:1212953/151‑1 (MQ=255)
                                                         cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATa                                                                                              <  2:1110602/151‑1 (MQ=255)
                                                              tttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TTTAAAAACACCC‑ATT‑AACCTCAAAAAAAAAAAAAAAATCCAG‑AATTA‑TTACTTTACCaaaaaaaaaaaa                                                                                          >  1:1750279/1‑140 (MQ=255)
                                                               ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACaa                                                                                          <  2:1440157/149‑1 (MQ=255)
                                                               ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGATA‑TTTAAAAACAAACAATA‑AACCTCAAAA‑AAAAACAAAAATCTAA‑AATTA‑TTAATTAACCCAAAAAAAACaaa                                                                                         >  1:2090013/1‑149 (MQ=255)
                                                                tATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TTTAAAAAAAACC‑ATT‑AACCCCAAAAAAAAAAAAAAAATCCAGAATTTT‑TTATTTAACaaaaaaaaaaaa                                                                                           >  1:1359884/1‑139 (MQ=255)
                                                                 aTCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TTTAAAAACACTC‑ATT‑AGCCTCAAAAAAAAAAAAAAACTCCAG‑ATTTT‑TTACTTTAACAATGaaaaaaaaaatt                                                                                      >  1:1578032/1‑147 (MQ=255)
                                                                 aTCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCt                                                                                       <  1:2119567/150‑1 (MQ=255)
                                                                   cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TTTAAAAAAAATA‑CTT‑ATCCTCAAAAAAAAAAAAAAAATTCAG‑ATTTA‑TTTTTTAAACAATAAAAAAAAAACtt                                                                                      >  1:926238/1‑149 (MQ=255)
                                                                   cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAACAACCC‑TTT‑GACTCCAAAAAAAAAAAAAACTT‑CAT‑ATTTT‑TTTATTAAcccaaaaaaaaaaa                                                                                          >  1:41903/1‑133 (MQ=255)
                                                                   cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTaa                                                                                    <  1:1983548/151‑1 (MQ=255)
                                                                     aTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTaaaa                                                                                  <  1:903049/151‑1 (MQ=255)
                                                                         tCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TGTAAAAACACCC‑ATT‑AGCCTCAAAAAAAAAAAAAAAATCCAC‑AATTA‑TTTTTTTAACAAAAAATAAAAAACATaaaaaa                                                                                >  1:553911/1‑149 (MQ=255)
                                                                         tCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAat                                                                               <  2:1671987/150‑1 (MQ=255)
                                                                          cACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TTGAAAAACACTC‑ATT‑ATCCCAAAAAAAAAGACAAAAATCCAG‑AATTA‑TTATTTAAACAAAGaaaaaaaaaaaaaaaaaaaa                                                                              >  2:1633002/1‑138 (MQ=255)
                                                                            cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TTTAAAAACCCTC‑ATT‑AGCCTCAAAAAAAAAAAAAAAATTCAG‑AATTT‑TTACTTTAAAAAAGAAAAAAAAACTTtaaaaaaa                                                                              >  1:1841648/1‑146 (MQ=255)
                                                                              tAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACt                                                                          <  1:749934/150‑1 (MQ=255)
                                                                               aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TTTTAAAAAACTC‑ATT‑TTACTCAAAAAAAAAAAAAAAATTCAA‑AATTA‑TTTTTTTACCAAAGAAAAAAAAACCTaaaaaaaaaaatat                                                                        >  2:1809936/1‑143 (MQ=255)
                                                                               aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTg                                                                         <  2:1368494/150‑1 (MQ=255)
                                                                               aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGt                                                                        <  1:1213737/151‑1 (MQ=255)
                                                                                  gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTAt                                                                     <  1:414460/151‑1 (MQ=255)
                                                                                    aTGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TGTAAAAACACAC‑ATT‑TACCTCAAAAAAAAAAAAAAATTCCCG‑ATTTT‑TTTCTTTAACAAAAAAAAAAAAAATTAAAAAAAATAATGTtttt                                                                    >  1:1084068/1‑150 (MQ=255)
                                                                                     tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TTTAAAAACACTC‑ATT‑AATCTCAAAAAAAAAAAAAACTTTCCG‑ATTTATTTATTTAACAAAT‑AATAACAACAATAAAAAAAAAAATGTTaattt                                                                  >  2:112325/1‑147 (MQ=255)
                                                                                      gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTa                                                                 <  2:1133471/151‑1 (MQ=255)
                                                                                      gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGACTCAAAAAAAAAAAAAAATTCCAG‑ATTTT‑TTATTTTACCAATGAATAACAAACTTAAAAAAAATACTGTTAtttt                                                                  >  1:1457328/1‑148 (MQ=255)
                                                                                         cTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TTTAAAAAAAATC‑ATT‑ATCCTCAAAAAAAAAAAAAAATTTCAG‑AATTA‑TTACTTTAACAATGAAAAAAAAACATAAAAAATATAATTTTATTTTaa                                                                >  2:814039/1‑149 (MQ=255)
                                                                                          tGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TTTAAAAAAACTC‑CTT‑ATCCTCAAAACAAAAAAAAAAATCCAG‑ATTTT‑TTAATTTACCAATGAATAAAAAAATTAAAAAAAATAATGTtttttt                                                                  >  2:2162358/1‑144 (MQ=255)
                                                                                          tGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TTTAAAAAAACTC‑ATT‑ATCCTCAAAAAAAAAAAAAAACTTCAA‑ATTTA‑TTTTTTTAACAAAGAATAAAAAAATTAAAAAAaataattttttttt                                                                  >  2:1000052/1‑136 (MQ=255)
                                                                                           ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAg                                                              <  2:38108/149‑1 (MQ=255)
                                                                                           ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGa                                                             <  2:72577/150‑1 (MQ=255)
                                                                                                      ttCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TTTAAAAACACCC‑ATT‑AGCCCCCAAAAAAAAAAAAACTTCCAG‑ATTTT‑TTACTTAAACAAAGAATAAAAAACTTAAAAAAAATACTGTTATTTTTAAAAAAAAAATaa                                                    >  1:190481/1‑148 (MQ=255)
                                                                                                        cATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TTTAAAAACCCTC‑ATT‑AGCCTCCAAAAAAAAACAAACATCCCG‑ATTTT‑TTTCTTTACCAAAGAATAAAAAACTTTAAAAAAATAATGTTATTTTAAAAAAAAAAATaaaaa                                                 >  1:675942/1‑146 (MQ=255)
                                                                                                        cATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAgtc                                               <  2:1022239/151‑3 (MQ=255)
                                                                                                         aTAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCa                                              <  2:1402955/151‑1 (MQ=255)
                                                                                                          tAAGAACTATTCATCAACAGCGTATAGAGGCGGTTT‑TGTAAAAACACTC‑ATT‑AACCTCAAAAAAAAAACAAAATTCCCC‑ATTTT‑TTTATTTACCAATGAAAAAAAAACTTAAAAAAAAAAATGTTTTTTTTAAAAAAAAAATaaaa                                                  >  1:90924/1‑144 (MQ=255)
                                                                                                            aGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTa                                           <  1:959166/151‑1 (MQ=255)
                                                                                                             gAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTaa                                          <  2:1088317/151‑1 (MQ=255)
                                                                                                             gAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTaa                                          <  1:103043/151‑1 (MQ=255)
                                                                                                              aaCTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTTAAAAAACCC‑CTTAATCCTACAAAAAAAAAAAAACATCACT‑ATTT‑‑TTAATTTAAAAATAAATAACAAAATTAAAAAAAAAACTTATATTTTAAAAAAAAAAATaaaaaacaataat                                         >  2:866642/1‑140 (MQ=255)
                                                                                                               aCTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAAtt                                        <  1:44612/151‑1 (MQ=255)
                                                                                                                cTATTCATCAACAGCGTATAGAGGCGGTTT‑TGTAAAAACACCC‑ATT‑AGCCTCAAAACAAAAAAAAACTTCCAG‑ATTTT‑TTATTTAAACAATGAATAACAAACTTAAAAAAAATAATGTTTTTTTTAAAAAAAAAATAAAAAGGATTAAtt                                        >  2:839278/1‑150 (MQ=255)
                                                                                                                    tcatcaACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAat                                    <  2:1286792/150‑1 (MQ=255)
                                                                                                                    tcatcaACAGCGTATAGAGGCGGTTA‑TGTAAAAAAACTC‑ATT‑ATCCTCAAAAAAAAAAAAAAAATCCCG‑ATTTA‑TTACTTTAACAAAGAAAAAAAAAATAAAAAAAAAAAATTTTTTTTTTAAAAAAAAAATAAAAAGAAATAATTTaa                                     >  2:345430/1‑149 (MQ=255)
                                                                                                                      atcaACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGATAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATAtt                                 <  2:492861/151‑1 (MQ=255)
                                                                                                                        caACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTa                                <  1:262505/150‑1 (MQ=255)
                                                                                                                        caACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTa                                <  2:706272/150‑1 (MQ=255)
                                                                                                                          aCAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaa                             <  2:44508/151‑1 (MQ=255)
                                                                                                                           cAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaaa                            <  2:1700238/151‑1 (MQ=255)
                                                                                                                             gCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaaaaa                          <  2:1679887/151‑1 (MQ=255)
                                                                                                                              cGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaat                         <  1:277141/151‑1 (MQ=255)
                                                                                                                                     agGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataat                      >  2:1560821/1‑147 (MQ=255)
                                                                                                                                       gCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTa                <  2:1185200/151‑1 (MQ=255)
                                                                                                                                          gTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaaaa             <  2:653297/151‑1 (MQ=255)
                                                                                                                                          gTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaaaa             <  2:859684/151‑1 (MQ=255)
                                                                                                                                               tGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTc         >  1:1698856/1‑151 (MQ=255)
                                                                                                                                                  aaaaCCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCtt       >  1:117056/1‑150 (MQ=255)
                                                                                                                                                    aaCCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTACCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAc    <  2:173048/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                          
TTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTA‑TGTAAAACCACTC‑ATT‑AGCCTCAAAACAAAGACAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGA  >  NC_000913/2903543‑2903842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑