Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,2770AC42.5% 199.2 / 48.7 121V274G (GTA→GGA) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (64/5);  new base C (0/51);  total (64/57)
Fisher's exact test for biased strand distribution p-value = 1.52e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAAT  >  NC_000913/3443127‑3443423
                                                                                                                                                       |                                                                                                                                                  
gCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTa                                                                                                                                                    <  1:1277943/151‑1 (MQ=255)
  aGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAcc                                                                                                                                                  >  1:1025973/1‑151 (MQ=255)
    aCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAcccc                                                                                                                                                <  1:1053188/151‑1 (MQ=255)
     cGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAccccc                                                                                                                                               <  2:970158/151‑1 (MQ=255)
      gCATAGAGTAACACATAAAGCGGTTGCCCGGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCGAACCAGTACCGCCCCCGAACCAGGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCCAAGATTGCCGGGATTAcccca                                                                                                                                               <  2:203441/150‑2 (MQ=255)
      gCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCg                                                                                                                                              <  2:2172175/151‑1 (MQ=255)
      gCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCg                                                                                                                                              >  1:1160893/1‑151 (MQ=255)
         tAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGcc                                                                                                                                            >  2:1115066/1‑150 (MQ=255)
         tAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGcc                                                                                                                                            >  2:210017/1‑150 (MQ=255)
         tAGAGTAACACAGAAAGCGGTTGCCCAGGCTCCAAATACAGCGAAATTGTTGTCAACCAGTTCCAACCAGTACCGCCCCCGAACCATGGCGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGCATTTCCCCCGcc                                                                                                                                            <  2:486303/150‑1 (MQ=255)
          agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCat                                                                                                                                          >  1:417070/1‑151 (MQ=255)
                 cacaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACt                                                                                                                                   >  1:1127030/1‑151 (MQ=255)
                     tAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTc                                                                                                                                >  2:107223/1‑150 (MQ=255)
                     tAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTc                                                                                                                                >  1:715035/1‑150 (MQ=255)
                     aaaggCGTTTGCCAGGGCTGCAAATACAGAAAAATTGTTGTCAGCCATTCCAAACAAGTCCCCCCCCCGAACCAGGCCGCGATGGCCGCGGGAAACAAAATAATACGGGAAGCGAATATTGCGGGGATTCCCCCCGCCATATTCACTTTCa                                                                                                                               <  1:1050995/147‑1 (MQ=255)
                      caaGCGGTCGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTc                                                                                                                                >  2:1022547/2‑149 (MQ=255)
                       aaGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGc                                                                                                                             >  2:1342719/1‑151 (MQ=255)
                           ggTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGt                                                                                                                          >  2:1386754/1‑150 (MQ=255)
                           ggTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGt                                                                                                                          >  1:1874088/1‑150 (MQ=255)
                              tGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTaaa                                                                                                                       >  2:1432427/1‑150 (MQ=255)
                              tGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                      >  1:473281/1‑151 (MQ=255)
                                cccAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgt                                                                                                                    >  1:936133/1‑151 (MQ=255)
                                cccAGCCTGAAAATACAGAAAATTTGTTGTCGGCCAGTCCCACCCAGTCCCCCCCCCGACCCATGACGCGATGGTCGCGGGAAAAAAAATAATACTGGAAGCGAAGATTGGGTGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                      <  2:1287612/149‑1 (MQ=255)
                                 ccgggCTGAAAATACAGCGAAATGGTTGTCAGCCAGTCCCACCCGGTCCCGCCCCCGACCCAGGGGGGGGTGGTCGCGGGGAAAAGAATAATGCGGGAGGGGAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTaa                                                                                                                        <  1:375472/143‑1 (MQ=255)
                                   aagCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAAAGAATAATACTGAAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgtg                                                                                                                 >  2:547186/3‑151 (MQ=255)
                                     gctaaaaaaaaaGCGAATTTGTTGCCCGCCATTTCAACCCATTCCCCCCCCCAAACCATGACGGGTTGGTCGCGGGAAACAAAAAAATACGGAAAGCAAAGATTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                               <  1:397221/140‑1 (MQ=255)
                                     gCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                               >  2:1009397/1‑151 (MQ=255)
                                     gCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                               >  1:127000/1‑151 (MQ=255)
                                        gCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                            >  1:973907/1‑151 (MQ=255)
                                         cAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgt                                                                                                           >  2:2119192/1‑151 (MQ=255)
                                          aaaTACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgtg                                                                                                          >  2:886454/1‑151 (MQ=255)
                                          aaaTACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgtg                                                                                                          >  1:81386/1‑151 (MQ=255)
                                          aaaGACAGCAAAGTTTTGGGCAGCCAGTACCAACACATACCGCCCCAGAACCGTGACGGGATGTTCGAGGGAAACAAAATAATGCAGGAATGGAAGGGTGCGGTAATTCTCGCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgtg                                                                                                          <  2:1585929/151‑1 (MQ=255)
                                           aaTACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                         >  1:2002599/1‑151 (MQ=255)
                                            aaaCAGCAAAATTGTTGGCAGCCAGTTCCACCCAGTCCCGCCCCCGAACCATGCCGCGAGGGTCGCCGGGAACAGAAAAATGCTGGAAGGGAAGATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgca                                                                                                        <  2:2121061/149‑1 (MQ=255)
                                             tACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                         >  2:305819/1‑149 (MQ=255)
                                                agtgaatttttggccAGCCATTCCCACCCAGTACCCCCCCCGAACCATGGCGCGAGGGTCGCCGGAAAAAAAAAAATGCTGAAGGGGAAATTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                      <  2:1847461/135‑1 (MQ=255)
                                                 gCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                    >  2:1193095/1‑150 (MQ=255)
                                                    aatttgttgTCACCCGTTTCCACCCAGTCCCCCCCCCGACCAATGACGCGAGGGTCGCGGGAAACAGAATAATCCTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATa                                                                                                   <  1:1436212/145‑1 (MQ=255)
                                                    aatttgttgCCCGCCATTCCAAACCAGACCCGCCCCCGACCCATGCCGCGATGGCCGCGGGGAACATAATATTAGGGAAGGGAAGGTGTGCGGGTATACCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGa                                                                                                 <  2:887578/147‑1 (MQ=255)
                                                     atttttggccAGCCAGTCCCAACCAGTCCCCCCCCCGACCCATGACGCGATGGTCGCCGGAAACAAAAAAATACTGGAAGCAAAGATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                                                               <  2:714935/142‑1 (MQ=255)
                                                       tttttgccgcccATTTCCACCCATCACCGCCCCAGAACCGTGACGCGGTGGTCGCCGGAAACAAAAAAATACTGGAGGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                                                               <  2:1312971/139‑1 (MQ=255)
                                                        tgttgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                             >  1:1855268/1‑150 (MQ=255)
                                                        tgttgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                             >  1:1927374/1‑150 (MQ=255)
                                                        tgtgttCAGCCAGTTCCAACCAGCCCCCCCCCCGACCCATGAGGCGATGGTCGCGGGAAAAAAAATAATACTGGAAGCGAATTTTGGCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATa                                                                                                   <  2:1779943/139‑1 (MQ=255)
                                                         gttgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                           >  2:286813/1‑151 (MQ=255)
                                                          ttgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg                                                                                          >  1:1997283/1‑151 (MQ=255)
                                                             tCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGCTGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGAcc                                                                                       >  2:734362/1‑151 (MQ=255)
                                                                 ccattcccAACAAGTCCCCCCCCCAACCCATGACGGGGTGGTCGCGGGAAACAAAATAATACTGGAAGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg                                                                                          <  2:807121/138‑1 (MQ=255)
                                                                        cAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                            >  2:647905/1‑151 (MQ=255)
                                                                        cAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                            >  1:1599234/1‑151 (MQ=255)
                                                                           ccAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcg                                                                         >  1:631380/1‑151 (MQ=255)
                                                                             agcaCCGCCCCCAAACCAGGACGCGATGGTCGCGGGAAACAAAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgc                                                                        <  1:1938991/147‑1 (MQ=255)
                                                                                cccccccccAACCCATGCCGCGGGGTTCGCGGGGAACAAAAAAATACGGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                           <  2:2086006/140‑1 (MQ=255)
                                                                                 ccccccccAAACCATGGCGGGAGGGCCGGCGGGAAAAGAAAAATACGGGAAGCAAAGATGGCCGGATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                            <  2:1798234/139‑1 (MQ=255)
                                                                                 ccGCCCCCGAACCAGGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTa                                                                     <  1:1657073/149‑1 (MQ=255)
                                                                                  cGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                  >  2:92927/1‑151 (MQ=255)
                                                                                     ccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAc                                                               >  2:1010607/1‑151 (MQ=255)
                                                                                       ccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAcc                                                              >  2:368553/1‑150 (MQ=255)
                                                                                         gAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                            >  2:1951741/1‑150 (MQ=255)
                                                                                            ccATGCCGCGGTGGTCGCCGGAAAAAAAATAATACTGGAAGGGAAGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                            <  1:2122139/147‑1 (MQ=255)
                                                                                            ccATGACGCGATGGGCGCCGGAAACAAAATAATCCTGGAAGCGAAGATTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACca                                                             <  1:1525022/146‑1 (MQ=255)
                                                                                              aTGACGCGAGGGTCGCGGGAAAAAAAATAATACTGGAAGCGAAGTTTGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                         <  2:746615/148‑1 (MQ=255)
                                                                                                gACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggc                                                    >  2:816885/1‑151 (MQ=255)
                                                                                                   gcgATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGtt                                                 >  2:523272/1‑151 (MQ=255)
                                                                                                     gATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                                >  1:405336/1‑150 (MQ=255)
                                                                                                        ggTCGCGGGAAAAAGAATAATGCGGGAAGCGAAGATGGCCGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGa                                               <  2:1319458/148‑1 (MQ=255)
                                                                                                         gTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCAc                                           >  2:444085/1‑151 (MQ=255)
                                                                                                          tCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACg                                          >  1:855153/1‑151 (MQ=255)
                                                                                                           cgcgGGGAAAAAAATAATACGGGAAGGGAATATGGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGc                                         <  1:722657/151‑1 (MQ=255)
                                                                                                           cGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGc                                         >  1:1041927/1‑151 (MQ=255)
                                                                                                                  aaCAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                   >  1:296652/1‑150 (MQ=255)
                                                                                                                   aaaaaataatACTGAAAGCAAAATTGGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACaaa                                 <  2:2100201/147‑1 (MQ=255)
                                                                                                                   aCATAATAATACGGGAAGAGAGGTTTGCAGGCATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCt                                        <  2:1165891/144‑1 (MQ=255)
                                                                                                                    cAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTGAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                >  2:1451981/1‑151 (MQ=255)
                                                                                                                     aaaataatACTGGAAGCGAAAATGGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                               <  2:773194/149‑1 (MQ=255)
                                                                                                                     aaaataatACTGGAAGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                               <  1:1377155/149‑1 (MQ=255)
                                                                                                                     aaaataaaACGGAAAGCGAAGATGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                               <  1:2021653/149‑1 (MQ=255)
                                                                                                                      gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                              >  1:1763183/1‑151 (MQ=255)
                                                                                                                       aaaaataCGGGAAGGAAAGATTGCGGGATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                             <  2:1150585/148‑1 (MQ=255)
                                                                                                                        aaaagccgggAAGCAAAGATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                              <  2:1882755/141‑1 (MQ=255)
                                                                                                                          aataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                           >  1:395973/1‑150 (MQ=255)
                                                                                                                             atgggACGCGAAGATTGCCGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACaaa                        <  2:2012289/147‑1 (MQ=255)
                                                                                                                             aCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                         >  1:1637851/1‑149 (MQ=255)
                                                                                                                             aCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                         >  2:710378/1‑149 (MQ=255)
                                                                                                                                       aaGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt                  <  1:1334955/146‑1 (MQ=255)
                                                                                                                                       aaGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg             <  1:2188367/151‑1 (MQ=255)
                                                                                                                                       aaGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCGCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc                 <  2:502987/147‑1 (MQ=255)
                                                                                                                                       aaGATGGCGGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt              <  2:1700067/150‑1 (MQ=255)
                                                                                                                                           ttGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa          <  1:1630422/150‑1 (MQ=255)
                                                                                                                                             tGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat        >  1:811688/1‑151 (MQ=255)
                                                                                                                                              gcgggcaTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat        <  2:417022/144‑1 (MQ=255)
                                                                                                                                               cggtgATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaaa         <  1:306241/144‑1 (MQ=255)
                                                                                                                                               cggggATACCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaaa         <  1:107239/146‑1 (MQ=255)
                                                                                                                                                 gggATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac      <  1:1170622/149‑1 (MQ=255)
                                                                                                                                                 gggATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact     <  2:195928/150‑1 (MQ=255)
                                                                                                                                                 ggaaTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat        <  1:141803/144‑1 (MQ=255)
                                                                                                                                                  ggATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatacta    >  2:1656327/1‑150 (MQ=255)
                                                                                                                                                   gATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaata       <  2:1542420/146‑1 (MQ=255)
                                                                                                                                                    aTTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaat  >  1:1639927/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                  
GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAAT  >  NC_000913/3443127‑3443423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: