Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,536,1970AT41.3% 170.9 / 101.1 128intergenic (+166/+417)yjhR/nanSpseudogene, helicase family; putative frameshift suppressor/9‑O‑acetyl N‑acetylneuraminic acid esterase
Reads supporting (aligned to +/- strand):  ref base A (5/69);  new base T (52/0);  total (59/69)
Fisher's exact test for biased strand distribution p-value = 4.71e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

ATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCC  >  NC_000913/4536051‑4536346
                                                                                                                                                   |                                                                                                                                                       
aTACAGGAGTAGTAAAGGGCATATTTTACCAATGTTCAGGCGACTTTCCTTTTTATTGCGTGTCGCCTTTCCTGATTATAAATCTTATTGTTTTCCTTATCATTTTCCTTTTCATAATGCGCCCTGGCTTAATGGCGGGTGC‑TTTTAgg                                                                                                                                                       <  2:1359016/149‑1 (MQ=255)
  aCAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑Tg                                                                                                                                                  <  2:2125735/151‑1 (MQ=255)
  aCAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑Tg                                                                                                                                                  <  1:92282/151‑1 (MQ=255)
  aCAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑Tg                                                                                                                                                  <  2:1227678/151‑1 (MQ=255)
   cAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑Tga                                                                                                                                                 <  2:1693486/151‑1 (MQ=255)
    aGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑Tgaa                                                                                                                                                <  2:420135/151‑1 (MQ=255)
       agtagtAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑Tgaaaga                                                                                                                                             <  2:655179/151‑1 (MQ=255)
        gtagtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑Tgaaagaa                                                                                                                                            <  2:281286/151‑1 (MQ=255)
        gtagtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑Tgaaagaa                                                                                                                                            <  2:462815/151‑1 (MQ=255)
          agtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑Tgaaagaaa                                                                                                                                           <  2:1663636/150‑1 (MQ=255)
          agtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGaaaa                                                                                                                                          <  1:712800/151‑1 (MQ=255)
           gtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTTT‑TTTTTTGTA‑TGAAAGaaaa                                                                                                                                          >  2:548217/1‑150 (MQ=255)
              aaGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATgca                                                                                                                                      <  1:1641493/151‑1 (MQ=255)
               aGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATgcaa                                                                                                                                     <  2:1144158/151‑1 (MQ=255)
               aGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGG‑TTTTTTGAA‑TGaaaaaaaaataa                                                                                                                                      >  2:479603/1‑146 (MQ=255)
               aGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGG‑TTTTTTGAA‑TGaaaaaaaaaaca                                                                                                                                      >  1:499749/1‑146 (MQ=255)
               aGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTACGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTTTGGA‑TGAAAGaaaaagaa                                                                                                                                      >  2:582535/1‑146 (MQ=255)
                 ggCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGG‑TTTTTTGGA‑TGaaaaaaaa                                                                                                                                          >  1:2133006/1‑144 (MQ=255)
                  gCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGG‑CTTTATGAAGTGaaaaaaaaacaa                                                                                                                                      >  1:1711158/1‑144 (MQ=255)
                   cATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGG‑TTTTTTGAA‑TGaaaaaaaaaaaaagca                                                                                                                                  >  1:567679/1‑142 (MQ=255)
                      atTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTTTGAA‑TTAAAGAAAATgcaagcaaaa                                                                                                                               >  1:1383352/1‑148 (MQ=255)
                      atTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCaataa                                                                                                                              <  2:278104/151‑1 (MQ=255)
                         ttACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGG‑TTTTTTGGA‑TGAAAGAAAAAAAAAGAaataa                                                                                                                              >  2:352149/1‑148 (MQ=255)
                          tACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAataatat                                                                                                                           <  1:55004/150‑1 (MQ=255)
                          tACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGG‑TTTTTGGGA‑TGAAAAAAAAAGCAAGCaaaaaaa                                                                                                                            >  1:886158/1‑147 (MQ=255)
                           aCCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAataatataa                                                                                                                         <  1:1268392/151‑1 (MQ=255)
                            ccAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTTTGAA‑TGAAAAAAAATACAAGCAaaaata                                                                                                                            >  2:1595133/1‑147 (MQ=255)
                            ccAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAataatataat                                                                                                                        <  2:372330/151‑1 (MQ=255)
                             cAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGG‑TTTTTTGAA‑TGAAAGAAAAAACAAGCaataaaaaaa                                                                                                                         >  1:1271717/1‑144 (MQ=255)
                              aaTGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATAtaat‑aa                                                                                                                     <  2:1988346/151‑1 (MQ=255)
                                 gTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATg                                                                                                                   <  2:719031/150‑1 (MQ=255)
                                 gTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGC‑TTTTTTGAA‑TGAAAGAAAAAGCAAAAAAAAATAtaat‑aa                                                                                                                     >  1:453782/1‑148 (MQ=255)
                                    aaGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGG‑TTTTTTGGA‑AGAAAGAAAAAGAAAGCAAaaaaataaa‑aataggc                                                                                                                >  2:1785272/1‑146 (MQ=255)
                                    aaGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGcc                                                                                                                <  1:470221/150‑1 (MQ=255)
                                       gCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAAt                                                                                                            <  1:279236/151‑1 (MQ=255)
                                        cGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAAtt                                                                                                           <  1:1168401/151‑1 (MQ=255)
                                        cGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAAtt                                                                                                           <  1:270196/151‑1 (MQ=255)
                                        cGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAAtt                                                                                                           <  1:1745041/151‑1 (MQ=255)
                                         gACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAAttt                                                                                                          <  1:278998/151‑1 (MQ=255)
                                            tttCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGC‑TTTTTTGGA‑TGAAAAAAAAAGCAAACAATAAAATAAA‑AAAAGGCTAAtttttt                                                                                                       >  1:29654/1‑151 (MQ=255)
                                             ttCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCtt                                                                                                       <  2:2000822/150‑1 (MQ=255)
                                             ttCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCt                                                                                                        <  1:948589/149‑1 (MQ=255)
                                              tCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGC‑TTTTTTGTA‑TTAAAAAAAAAGCAAGCAATAATATAAT‑AATTGGCTTATtttttt                                                                                                      >  2:990005/1‑150 (MQ=255)
                                               ccGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGa                                                                                                    <  2:2191678/151‑1 (MQ=255)
                                                 gTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAAc                                                                                                  <  2:939693/151‑1 (MQ=255)
                                                      aTTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAAcatcat                                                                                             <  1:1807944/151‑1 (MQ=255)
                                                        tGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCAtt                                                                                            <  1:827825/150‑1 (MQ=255)
                                                          cGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAg                                                                                         <  1:948070/151‑1 (MQ=255)
                                                               cGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGCGC‑CTTTTGGCA‑TGAAAAAAAAAGCAAAGAAAAAAATAAT‑AATAGGCTCAATTTTTTTAAAATCATTAAAAatat                                                                                    >  1:1059226/1‑151 (MQ=255)
                                                                    ttCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGG‑TTTTTTGGA‑TGAAAGAAAAAGCAAGCAATAAAATAAT‑AATGGCCTCATTTTTTTTTACATCATTAAACATatcat                                                                                 >  1:906270/1‑149 (MQ=255)
                                                                    ttCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATatcatc                                                                                <  2:1131832/150‑1 (MQ=255)
                                                                       cTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGC‑TTTTTTGTA‑TGAAAAAAAAAGAGAACAATAATATAATAAAAGGCCT‑ATTTTTTTTTATATAATTAAACATatca                                                                                  >  2:603738/1‑145 (MQ=255)
                                                                           ttATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTTTGTA‑TGAAAAAAAAAAAAAGCAAAAATATAAT‑AATTGCCTCATTTTTTTTTAAAAAATTAAGCATATAATCATCGt                                                                           >  2:796078/1‑148 (MQ=255)
                                                                           ttATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGCGGG‑TTTTTTTGA‑TGAAAAAAAAAGAAACCAAAAAAATAAA‑AATTGCCTTATTTTTTTTAAAAACCTTAAGAAAATCATCATTGTCt                                                                         >  2:56321/1‑150 (MQ=255)
                                                                             ataAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGC‑TTTTTTGGA‑TAAAAAAAAAAAAAAACAAAAATATAAT‑AAAGGCCTAATTTTTTTTAAAAACATTAAACATAATAACATCGTCtttt                                                                      >  1:1715395/1‑151 (MQ=255)
                                                                              taAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGG‑TTTTTGTTT‑TTAAAAAAAAAGTAAGCAAAAATATAAA‑AAATGGCTAATTTTTTTTTTAATAATTAAAAATATCATCATAGTTTAttt                                                                     >  2:1612945/1‑151 (MQ=255)
                                                                              taAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGC‑TTTTTGGAA‑TGAAAAAAAAAGAAAGCAATAATATAAA‑AAATGCCTAATTTTTTTTAACAACATTAAACATATCATAATCGTCTAttt                                                                     >  1:1726663/1‑151 (MQ=255)
                                                                              taAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGACGG‑TTTTTTGTT‑TGAAAGAAAAAGTAAGGAATAATATAAT‑AATAGGATTATTTATTTTTATATCATTAAGCATATATACATAATCTAttt                                                                     >  2:626599/1‑151 (MQ=255)
                                                                                 aTCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTTTGTA‑TGAAAGAAAAAGCAAGACATAATAAAAT‑AAATGCCTAATTTCTTTTAACAACATTAAACATATAATCTTCATCTAttttc                                                                   >  2:1931799/1‑148 (MQ=255)
                                                                                  tCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑CTTTTGGGA‑TGAAAAAAAAAACAAACAAAAAAATAAA‑AATAGCCTAATTTTTTTGTAAATCATTTAACATATTATCATCGTCTAtttt                                                                    >  1:581064/1‑147 (MQ=255)
                                                                                     tATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCa                                                                 <  2:487263/148‑1 (MQ=255)
                                                                                     tATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAg                                                                <  1:739449/149‑1 (MQ=255)
                                                                                       ttGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGG‑TTTTTGGAA‑TGAAAGAAAAAGCAAACAATAATATAAT‑AAATGCCTAATTTTTTTTTACATCATTAAGCATATCATCATCGTCTATTTTCCAGaa                                                              >  1:1025423/1‑149 (MQ=255)
                                                                                          tttACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTTTGGT‑TTAAAAAAAAAGGAAGGAATAAAATAAA‑AATGGGCTTATTTTTTTGTAAAAAATTTAACATATAATCATTGTCTATTTTcc                                                                  >  2:779865/1‑142 (MQ=255)
                                                                                           ttACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGt                                                         <  1:160881/150‑1 (MQ=255)
                                                                                           ttACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGt                                                         <  1:233280/150‑1 (MQ=255)
                                                                                           ttACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGt                                                         <  1:1910187/150‑1 (MQ=255)
                                                                                            tACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTc                                                        <  2:1518604/150‑1 (MQ=255)
                                                                                              ccTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGAc                                                     <  2:719960/151‑1 (MQ=255)
                                                                                                tAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGT‑TTTTTTGGT‑AGAAAAAAAAAGCAAGCAAAAATATAAA‑AAATTGCTAATTTTTTTTATAATAATTTAAAATATACTCATTCTCATTTTTCCAGAATAAGTCGAAtt                                                   >  2:1332086/1‑151 (MQ=255)
                                                                                                tAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGC‑TTTTTGGGA‑TGAAAGAAAAAGCAAGCAAAAATATAAT‑AATGGCCTAATTTTTTTTAAAATCATTAAACATATCATCATCGTCTATTTTCCAGAATCCGTCGACtt                                                   >  1:2169426/1‑151 (MQ=255)
                                                                                                 aaTCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGT‑TTTTTGGTA‑TTAAAGAAAAAGCAAGAAAAAAAATAAA‑AAAAGACTAATTTTTTTTAAAATAAATTAACATATAATAATCGTCTATTTTTCAGAATTCCTCCACtt                                                   >  2:1512875/1‑150 (MQ=255)
                                                                                                 aaTCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACtt                                                   <  1:1752194/150‑1 (MQ=255)
                                                                                                    cATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATaa                                               <  1:514465/151‑1 (MQ=255)
                                                                                                       tttCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATc                                             <  1:1004242/150‑1 (MQ=255)
                                                                                                        ttCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCg                                            <  1:1101539/150‑1 (MQ=255)
                                                                                                        ttCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGG‑GTTTTTTGT‑GGAAAAAAAAACCAAGAAAAAAAATAAA‑AATGGGCTAAATTTTTTTAACAAAATTAAAAAAATTAACATCGTCTATTTTTCAGAATACGTccccctatatta                                             >  2:1551358/1‑138 (MQ=255)
                                                                                                         tCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGG‑TTTTTGGGA‑AGAAAAAAAAAGCAAACAAAAAAATAAA‑AAAAGGCCAATTTTTTTTTAAAACATTAAACATATCATCATCGTCTATTTTTCCCAAACCGTCCACTTATAAttccc                                          >  1:1563089/1‑147 (MQ=255)
                                                                                                          ccTTTGCATAATGCGCCTGGGATTAATGGCGGGGGC‑TTTTTTGAA‑TGAAAAAAAAAACAAACAAAAATATAAA‑AAAAGGCTAAATTTTTTTAACATAATTAAACATATCATCATCGTCTATTTTTCAGAAACCGTCGACTTTTAATCTcc                                          >  1:1379898/1‑150 (MQ=255)
                                                                                                             ttGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGa                                       <  1:2090393/150‑1 (MQ=255)
                                                                                                              tGCATAATGCGCCTGGGATTAATGGCGGGGGC‑TTTTTGGGA‑TGAAAGAAAAAGCAAACAATAATATAAA‑AAATGCCCAATTTTTTTTTACATCATTAAGCATATCATCATCGTCTATTTTCCCGAAACCCTCGACTTATAATCGCCCGaa                                      >  1:2174959/1‑150 (MQ=255)
                                                                                                                cATAATGCGCCTGGGATTAATGGCGGGTGG‑TTTTTGGTA‑TGAAAAAAAAAGCAAGCAAAAATATAAT‑AAAGGGCTAATTTTTTTTTAAATCATTCAACATAACATCATAGTCTATTTTTCAGAAACCGTCCACTTTTAATTTCCAGAAAgg                                   >  2:532245/1‑151 (MQ=255)
                                                                                                                  tAATGCGCCTGGGATTAATGGCGGGGGCTTTTTTGGAA‑GGAAA‑AAAAAGCAAACAAAAATATAAT‑AATAGCCTAATTTTTTTTAAAAACATTAAACATATTATCATCGTCTATTTTTCAGAAACCGCCGACTTTTAAtttccacaaagg                                   >  1:229684/1‑138 (MQ=255)
                                                                                                                    aTGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCata                               <  2:1841322/151‑1 (MQ=255)
                                                                                                                     tGCGCCTGGGATTAATGGCGGGGGG‑TTTTTTGGA‑TGAAAGAAAAAGGAAGCAAAAATATAAA‑AAAGGCCTAATTTTTTTTTACATTATTAAACAAATCATCATCGTCTTTTTTCCCGAAACCGTCGAACTATAATTCCCAGaa                                      >  1:198234/1‑143 (MQ=255)
                                                                                                                         ccTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAgg                          <  1:1021972/151‑1 (MQ=255)
                                                                                                                          cTGGGATTAATGGCGGGTGC‑TTTTTTGGA‑TGAAAGAAAAAGCAAGAAAAAATATAAT‑AATTGCCTAATTTTTTTTAACATCATTAAGCATAACATCATCGTCTATTTTCCAGAATCCGTCGACTTATAATCTCCAGAAAAGCatat                              >  2:1396413/1‑146 (MQ=255)
                                                                                                                            gggATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGAc                        <  2:1397596/150‑1 (MQ=255)
                                                                                                                               aTTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGtg                    <  1:1370307/151‑1 (MQ=255)
                                                                                                                                 tAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGtgc                   <  1:1336430/150‑1 (MQ=255)
                                                                                                                                  aaTGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGtgctg                 <  1:101782/151‑1 (MQ=255)
                                                                                                                                   aTGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGtgctgc                <  2:529360/151‑1 (MQ=255)
                                                                                                                                    tGGCGGCTGC‑TTTTCAGAA‑TGAAAGAAAATCCAAGCAATTATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTCTAAACGCCAGAACGGCATATCAGGACGGtgatgc                >  2:682163/1‑150 (MQ=255)
                                                                                                                                      gCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCg               >  2:607671/1‑149 (MQ=255)
                                                                                                                                      gCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGa              >  2:663988/1‑150 (MQ=255)
                                                                                                                                       cGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAAc            <  1:1498587/151‑1 (MQ=255)
                                                                                                                                          gTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCt         <  1:1030893/151‑1 (MQ=255)
                                                                                                                                            gC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCtgt       >  1:1840178/1‑151 (MQ=255)
                                                                                                                                                tttAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTgg     <  1:2165270/150‑1 (MQ=255)
                                                                                                                                                tttAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGc    >  1:746557/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                       
ATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGC‑TTTTAGGAA‑TGAAAGAAAATGCAAGCAATAATATAAT‑AATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCC  >  NC_000913/4536051‑4536346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑