BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,824,137	G→T	D580Y (GAC→TAC)	spoT →	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,824,137	0	G	T	100.0%	434.7 / NA	111	D580Y (GAC→TAC)	spoT	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (69/42); total (69/42)

Only 100 of 111 total aligned reads displayed.

AATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAA  >  NC_000913/3823995‑3824283

aaTGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGAtt                                                                                                                                            >  2:1288938/1‑151 (MQ=255)

  tGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTAt                                                                                                                                          >  2:1198141/1‑151 (MQ=255)

  tGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTAt                                                                                                                                          >  2:1477352/1‑151 (MQ=255)

  tGAGCGTGGCGGTCGCGAAAAATCTGCAACATGCGGACGCCTCCATTCCACCGGCACCCCAAAACCACGGACATCTGCCCATTAAAGCTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTTTTCCTGGCTACCCGactct                                                                                                                                          >  2:943739/1‑147 (MQ=255)

         ggtggtCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCa                                                                                                                                    <  2:833398/150‑1 (MQ=255)

         ggtggtCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCAc                                                                                                                                   <  1:305023/151‑1 (MQ=255)

         ggtggtCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCAc                                                                                                                                   <  1:2106206/151‑1 (MQ=255)

         ggtggtCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCAc                                                                                                                                   <  1:1914618/151‑1 (MQ=255)

          gtggtCGCGACAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACg                                                                                                                                  >  1:1355230/1‑151 (MQ=255)

          gtggtCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACg                                                                                                                                  >  2:44676/1‑151 (MQ=255)

               cgcgAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGc                                                                                                                             <  2:1356310/151‑1 (MQ=255)

                gcgAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGcc                                                                                                                            >  1:994022/1‑151 (MQ=255)

                 cgAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGccc                                                                                                                           <  1:1188740/151‑1 (MQ=255)

                    aaaaTCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCgg                                                                                                                        <  1:1445401/151‑1 (MQ=255)

                     aaaTCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGt                                                                                                                       <  2:2127181/151‑1 (MQ=255)

                       aTCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTa                                                                                                                      >  2:1336229/1‑150 (MQ=255)

                         cTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTaagg                                                                                                                   >  1:163098/1‑149 (MQ=255)

                          tGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAgg                                                                                                                  >  1:939373/1‑151 (MQ=255)

                           gCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTaaa                                                                                                                    <  2:872561/148‑1 (MQ=255)

                             aaCATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTTCTGATCTCCTTTGCGAAAGGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTc                                                                                                                <  2:2132484/150‑1 (MQ=255)

                             aaCATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCt                                                                                                               >  1:1657016/1‑151 (MQ=255)

                               cATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTgg                                                                                                             <  1:467338/151‑1 (MQ=255)

                                  ggggACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTg                                                                                                           <  2:2146462/150‑1 (MQ=255)

                                   gggACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTg                                                                                                           >  2:2064038/1‑149 (MQ=255)

                                   gggACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGAt                                                                                                         <  1:2039163/151‑1 (MQ=255)

                                      aCGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATcca                                                                                                      <  1:1498129/151‑1 (MQ=255)

                                         ccTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATccacc                                                                                                    >  2:1222869/1‑150 (MQ=255)

                                             cATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGa                                                                                                >  2:1892425/1‑150 (MQ=255)

                                              aTTCCACAGGCAACCGAAAGCAAAGGACATCTGCACATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCATATTCCTGGCTACCCGATTATCGCCCACGTCAGCCGCGGTAAAGGTCTGGTGATCCACCATGAAt                                                                                              >  2:666396/1‑151 (MQ=255)

                                                tCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATcc                                                                                            >  2:698748/1‑151 (MQ=255)

                                                  cACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTg                                                                                          <  2:1386640/151‑1 (MQ=255)

                                                    ccGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGcc                                                                                        <  2:276549/151‑1 (MQ=255)

                                                     cGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCg                                                                                       >  1:1993913/1‑151 (MQ=255)

                                                     cGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCg                                                                                       <  1:1804741/151‑1 (MQ=255)

                                                     cGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCg                                                                                       >  2:1319056/1‑151 (MQ=255)

                                                      ggCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGt                                                                                      >  2:1082375/1‑151 (MQ=255)

                                                      ggCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGt                                                                                      >  1:2087013/1‑151 (MQ=255)

                                                       gCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGt                                                                                      >  1:2002313/1‑150 (MQ=255)

                                                        cAACCCAAAGCCACGGACATCTGCCCATTAAAGTTCCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCCCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTa                                                                                     <  2:1307623/150‑1 (MQ=255)

                                                         aaCCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAat                                                                                   >  1:613535/1‑151 (MQ=255)

                                                          aCCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAat                                                                                   >  2:1400255/1‑150 (MQ=255)

                                                              aaaGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCg                                                                              >  2:1900441/1‑151 (MQ=255)

                                                               aaGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGTTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCg                                                                              >  2:295666/1‑150 (MQ=255)

                                                               aaGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGt                                                                             >  1:754984/1‑151 (MQ=255)

                                                                aGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTg                                                                            >  1:1127331/1‑151 (MQ=255)

                                                                aGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTg                                                                            >  1:1140266/1‑151 (MQ=255)

                                                                aGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTg                                                                            >  2:2081786/1‑151 (MQ=255)

                                                                 gCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTgg                                                                           >  2:1320227/1‑151 (MQ=255)

                                                                   cACGGACATCTGCCCATTAAAGGTGCCGATGTCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCt                                                                         >  1:72838/1‑151 (MQ=255)

                                                                    aCGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCt                                                                         >  1:1531050/1‑150 (MQ=255)

                                                                     cGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTa                                                                        >  2:1483715/1‑150 (MQ=255)

                                                                      ggACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTAcc                                                                      >  1:1914762/1‑151 (MQ=255)

                                                                          aTCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGa                                                                   >  2:738430/1‑150 (MQ=255)

                                                                           tCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGaa                                                                  >  1:1060174/1‑150 (MQ=255)

                                                                              gCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAaga                                                               <  1:2047737/150‑1 (MQ=255)

                                                                              gCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAag                                                                <  1:1606283/149‑1 (MQ=255)

                                                                                ccATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGcc                                                            >  1:2058625/1‑151 (MQ=255)

                                                                                  aTTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGcc                                                            >  2:831450/1‑149 (MQ=255)

                                                                                    tAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCaga                                                         <  1:407297/150‑1 (MQ=255)

                                                                                    tAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCag                                                          <  2:2071727/149‑1 (MQ=255)

                                                                                     aaaGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCag                                                          >  1:1714361/1‑148 (MQ=255)

                                                                                      aaGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAgaga                                                       >  2:128169/1‑150 (MQ=255)

                                                                                            ccGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTAt                                                >  1:2088484/1‑151 (MQ=255)

                                                                                                aggCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGACTCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCt                                            >  2:693701/2‑151 (MQ=255)

                                                                                                     tGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTgg                                        >  1:1040849/1‑150 (MQ=255)

                                                                                                         gATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAAt                                     <  1:85443/149‑1 (MQ=255)

                                                                                                           tCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATggg                                  >  2:1287744/1‑150 (MQ=255)

                                                                                                               cTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATaa                              >  1:1955450/1‑150 (MQ=255)

                                                                                                               cTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATaa                              >  2:1806326/1‑150 (MQ=255)

                                                                                                                tttGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATaa                              >  2:1240333/1‑149 (MQ=255)

                                                                                                                 ttGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATaaa                             <  1:2168766/149‑1 (MQ=255)

                                                                                                                 ttGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATaa                              >  2:188830/1‑148 (MQ=255)

                                                                                                                 ttGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATaa                              >  1:1288082/1‑148 (MQ=255)

                                                                                                                 ttGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATaa                              >  2:671912/1‑148 (MQ=255)

                                                                                                                    cGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAagag                          <  2:1925491/149‑1 (MQ=255)

                                                                                                                     gAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCGCCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGAc                        <  2:1806595/150‑1 (MQ=255)

                                                                                                                     gAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACg                       <  1:17877/151‑1 (MQ=255)

                                                                                                                      aaaTGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACgg                      >  2:61910/1‑151 (MQ=255)

                                                                                                                      aaaTGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACg                       <  2:673167/150‑1 (MQ=255)

                                                                                                                        aTGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGgcg                    >  2:1572495/1‑151 (MQ=255)

                                                                                                                          gctgcCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCa                  <  1:1172250/151‑1 (MQ=255)

                                                                                                                          gctgcCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCa                  <  1:636425/151‑1 (MQ=255)

                                                                                                                             gccgccCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAgg                <  2:718321/150‑1 (MQ=255)

                                                                                                                              ccgccCTATTCCTGGCTACCCGATTATCGCCCCCGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAg              <  2:1234378/151‑1 (MQ=255)

                                                                                                                              ccgccCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGa               <  2:417075/150‑1 (MQ=255)

                                                                                                                                gccCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGtt            >  1:1912630/1‑151 (MQ=255)

                                                                                                                                 cccTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGtt            <  1:1919038/150‑1 (MQ=255)

                                                                                                                                 cccTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTtc           >  2:94703/1‑151 (MQ=255)

                                                                                                                                    tATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTtcat         >  2:382136/1‑150 (MQ=255)

                                                                                                                                     aTTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTtcatca       <  1:1198611/151‑1 (MQ=255)

                                                                                                                                        ccTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCAcc     <  2:419677/150‑1 (MQ=255)

                                                                                                                                        ccTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCAcc     >  2:2147450/1‑150 (MQ=255)

                                                                                                                                        ccTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCAcc     <  1:1909361/150‑1 (MQ=255)

                                                                                                                                        ccTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCg    >  1:110385/1‑151 (MQ=255)

                                                                                                                                         cTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCg    >  1:1391842/1‑150 (MQ=255)

                                                                                                                                         cTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCg    >  1:1235810/1‑150 (MQ=255)

                                                                                                                                         cTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCg    >  2:1166233/1‑150 (MQ=255)

                                                                                                                                          tGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGa   >  1:640820/1‑150 (MQ=255)

                                                                                                                                           ggCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGaa  <  1:1407669/150‑1 (MQ=255)

                                                                                                                                           ggCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGaa  <  2:2095922/150‑1 (MQ=255)

AATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAA  >  NC_000913/3823995‑3824283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`