Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,556,207 | 0 | T | C | 38.6% | 91.6 / 13.7 | 57 | S215G (AGT→GGT) | rtcA | RNA 3'‑terminal phosphate cyclase |
Reads supporting (aligned to +/- strand): ref base T (31/4); new base C (0/22); total (31/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.28e-12 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGG‑TGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACC > NC_000913/3556059‑3556357 | gCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGG‑TGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTcc < 1:717638/151‑1 (MQ=255) cAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGG‑TGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGc < 2:691102/151‑1 (MQ=255) aaCCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGG‑TGATATTTTCACTTTCGACTACAAGCGAGACGGTATTACCCGGCCCCTGGG‑CGCGCGGAAGGGTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCg > 1:104164/1‑149 (MQ=255) accTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGG‑TGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtg > 1:977692/1‑151 (MQ=255) ccTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGG‑TGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtgt > 2:298799/1‑151 (MQ=255) ggCACTGCGGCGCTTTCCACCGACAAAAAAAAGGGGTTCGG‑TGATTTTTGCACTGTCGACTACAAGGGAGACGTTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTAGAAATATTCTTTTCATGCAGGGAAAAC‑CTCCCCGCCAGTGTAg < 2:747341/149‑1 (MQ=255) cACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGG‑TGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGAt > 2:216805/1‑151 (MQ=255) gcTTTTCACCGACGACAAAAAAGCGTTCGG‑TGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTc > 1:791935/1‑151 (MQ=255) ttttCCCCGCCGAAAAAAAAGCGTTGGG‑TTATTTTTTCACTTCCGACTTCAAGCGAGCCGGTATTCCCCGCCCCCGGGT‑CGCGCGGCAGTTTATAAATTTTCTGTTC‑TTCAGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCt < 2:1035289/148‑1 (MQ=255) ttttCACCGACGACAAAAACGAGTTCGG‑TGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCATGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTc > 2:36940/1‑149 (MQ=255) tttcCACCGACGACAAAAAAGCGTTGGG‑TGATATTTTCCCTTCCGACTTCAAGCGAGAGGTTATTCCCCGCCCCCTGGT‑CGCGCGGCAGGTTATAAATATTCTTTTAATGCAGGAAAAAC‑CTCCCCGCCAGTGTAGCGATTTCACg < 1:80184/146‑1 (MQ=255) tttcCACCGACGAAAAAAAAGCGTTGGG‑TGATTTTTTCCCTTCCGCCTTAAAGCGAGACGTTATTCCCCGCCCCCTGGT‑CGCGCGGCAGGTTATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTc < 1:367331/149‑1 (MQ=255) acgacAAAAAAGCGTTCGG‑TGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgc > 1:451003/1‑151 (MQ=255) aaaaaaagGCGTTCGG‑TGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTCCCCGCCCCCGGGC‑CGCGCGGCAGTTTATAAATATTCTTTTCATGCAGGGAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg < 1:470506/149‑1 (MQ=255) aaaaaaaaGCGTTGGG‑TAATATTTTCACTTCCGACTCAAAGGGAGACGGTTTTCCCCGCCCCCGGGT‑CGCGCGGCAGGTTAGAAATTTTCTGTTCATGCAGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATg < 1:658459/144‑1 (MQ=255) aaaaaGC‑TTCGGTTAATATTTTCCTTTTCAACTACAGGTGAAACGGTATT‑CCCGGCCCCCGGTGCGGGCGACATTTTATAAATATTTTGTTCTCGCGGGAAAAAT‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGca < 1:91795/151‑1 (MQ=255) aaaaGCGTTCGG‑TGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTCCCCGCCCCCTGGC‑CGCGCGGCGGGTTATGAATATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcac < 1:802924/151‑1 (MQ=255) ttCGG‑TGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGG‑AAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt > 1:331108/1‑150 (MQ=255) gttGATTTTTCCTCTTTCGTCTACAAGCGAGACGGTTTTCCCCGGCCCCGGGG‑CGCGCGGCAGTTTTTGAATATTCGTTGCATGCGGGGAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTa < 2:638648/147‑1 (MQ=255) ttttCCCTTTCGCCTCCAAGGGAGCCGGTATTCCCCGGCCCCGGGT‑CGCGCGGCGGTTTATGAATTTTCTGTTCAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc < 1:930731/149‑1 (MQ=255) ttCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtct > 1:720457/1‑151 (MQ=255) tCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtctc > 1:844476/1‑151 (MQ=255) tCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑ATCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtct > 2:52172/1‑150 (MQ=255) cccTTTCGACTTCAAGCGAGACGGTATTCCCCGGCCCCGGGT‑CGCGCGGCGGTTTATGAATATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtct < 1:871838/147‑1 (MQ=255) cACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTcc > 1:134850/1‑151 (MQ=255) aCTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTcc > 1:1021763/1‑150 (MQ=255) tttAGACTTCAAGCCAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGCTATGAATATTCTGATCATGCACGGAACAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCCAATAGAACTTCTCCACg > 2:574234/1‑151 (MQ=255) gtCGACTACAAGGGAGGCGGTTTCACCCGGCCCCGGGG‑CGCGCGGCAGGTTATGAATATTCTGTTCAGGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACg < 2:656918/149‑1 (MQ=255) gACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATc > 1:217380/1‑151 (MQ=255) ttCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGc > 2:118913/1‑151 (MQ=255) cAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcac > 1:169766/1‑151 (MQ=255) aaGCGGGCCGGTTTTCCCCGCCCCCTGGC‑CGCGCGGCGGTTTATGAATATTCTGTCCATGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGc < 1:300937/148‑1 (MQ=255) aaGCGGGAGGTTATCCCCCGGCCCTGGGG‑CGCGCGGCAGGTTATAAATATTCTGTCCATGCAGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCt < 1:463315/146‑1 (MQ=255) aaGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcac > 2:680826/1‑150 (MQ=255) agaCGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATGTTCTGATCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt > 2:589484/1‑150 (MQ=255) gTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc > 1:989404/1‑151 (MQ=255) ccTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTg > 1:121214/1‑151 (MQ=255) ggggCAGTTTATAAATATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtg < 1:962821/147‑1 (MQ=255) gcggccggtagagAATTTTCGTTTCATGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACGTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAg < 2:137210/134‑1 (MQ=255) gcgGCAGGTTATAAATATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt < 1:161402/151‑1 (MQ=255) ggCAGTTTATGAATTTTCTTTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt < 1:966785/149‑1 (MQ=255) tGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc > 1:103086/1‑150 (MQ=255) tGAATATTCTGTTCATCCAGGGAAAAA‑CTCCCCGCCAGAGTAGCGATTTCACGCTCAGCAACATGGCGCGCCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAAGGTTCCCGCGCTCGCCAAGTGGCAAGGTGTTAAACCATGcc > 2:307000/1‑150 (MQ=255) tGAATATGCGGTTAATGCAGAAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa < 2:441400/146‑1 (MQ=255) gAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCAc > 1:992228/1‑151 (MQ=255) ttCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACggg < 1:59396/149‑1 (MQ=255) tccTGTTCA‑GGAGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa < 2:120957/143‑1 (MQ=255) tCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg > 1:747598/1‑150 (MQ=255) tCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg > 1:31988/1‑150 (MQ=255) tCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg > 1:348747/1‑150 (MQ=255) gTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc > 1:504319/1‑151 (MQ=255) caggcgggAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATAGCGCGGCACACCAGCTAAGAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTCCAAGGTGTTAAACGATGCCACGGGCGAGACt < 2:102222/144‑1 (MQ=255) gCAGAGAAAAA‑CTCCCCGCCAGTTTAGCGCTTTCACCCTCATCAACATAGCGCGGCACACCAGCTAATAGCACTTCCCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGACTCTCCGGGCGCGACTTCCGt > 2:1051264/1‑151 (MQ=255) ggaaaaac‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGc < 1:794027/148‑1 (MQ=255) aaaac‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGcc < 1:522389/149‑1 (MQ=255) aa‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCcaccac > 1:790691/1‑151 (MQ=255) cTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCacac > 2:426779/1‑151 (MQ=255) tCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACAcc > 1:559842/1‑151 (MQ=255) | GCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGG‑TGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACC > NC_000913/3556059‑3556357 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |