Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,332,791 | 0 | A | C | 43.9% | 94.2 / 17.8 | 57 | V161G (GTG→GGG) | basS | sensory histidine kinase in two‑component regulatory system with BasR |
Reads supporting (aligned to +/- strand): ref base A (32/0); new base C (0/25); total (32/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.01e-16 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGC > NC_000913/4332641‑4332933 | aCATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCa < 1:152215/151‑1 (MQ=255) gTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCAcccc > 1:996210/1‑151 (MQ=255) aCCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGc > 1:946501/1‑151 (MQ=255) ggAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCCGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGtt > 2:95821/1‑151 (MQ=255) agaaAATGCCTGCCCGGCGCGCGCCAGTTGAAGCAGCGGGGAGACGCTCT‑CCATCACCTAATCAACCGGTGCCACTAACGGTGCTACATCAAGGTGATGCTTTTTCGCCAGCAGTTCCAGAGGCAACCCCCCCCCCGCCAGTGGCGTTCGc < 2:273574/151‑1 (MQ=255) aaaaTGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGc > 1:194928/1‑149 (MQ=255) aaaTGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGt > 2:1025573/1‑151 (MQ=255) tGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCg > 2:307643/1‑151 (MQ=255) gACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGt > 2:1045017/1‑151 (MQ=255) cTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTgc > 1:728504/1‑151 (MQ=255) tGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTgcg > 1:8193/1‑151 (MQ=255) gTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTgcgc > 1:489078/1‑151 (MQ=255) aCGCGCCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTc > 2:962694/1‑150 (MQ=255) gcacgaGTAGCAGCAGGTGGGAGACGCTCC‑CCTTCTTCGAATCAGCCCGTGCCATTAATGTTGTTACACAAATGTGTTGCTTTTCCGCCAGCAGTCCAAGACGAAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGAc < 1:981926/140‑1 (MQ=255) cgcCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCAAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCg > 2:793496/1‑151 (MQ=255) cgaggcggggcagcGGGGAGCCG‑TCTCCCACCATTGGACCAAGCCGTCCCACTAACGGTGCTCCACCAATGGGTTGTGTTTCCGCCGGCGGTCCGAAGTGCAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTc < 2:124930/136‑1 (MQ=255) ccAGTGGCAGCAGCGGGGAGCCCCTCC‑CCATAATCTAACCAAGCCGTGCCACTAAGGGTGCTACATCAATGTGATGTTTTTTCCCAAGCAGTTCCGGATGCAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGt < 1:582894/151‑1 (MQ=255) ttGCAGCGGGGGGGAGCCCCTCC‑CCATCATCTAATCAAGCCGGCCCACAAACGGTGCTACATAAATGTGGTGCTTTTCCGCCAGCAGTCCCAGAGGCAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAg < 1:672376/143‑1 (MQ=255) tggcagcaGCGGGGAGCCCCTCT‑CCATCATCTGATCAAGCCGTGCCACTAAGGGTGCTACATAAATGTGATGCTTTTCCGCCAGCATTTCCAGATGAAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTaa < 1:21760/147‑1 (MQ=255) ctgcctcTGGAAGTCACTCT‑CCATCTTTGGCTCAAGCCGCCCCACTAACTGTGCTCCCTCTATGTCCTTCTTTTTCCCCAGCAGTCCAATCCGAAAGCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCAACGTCAGCGGTAaacaa < 2:259452/144‑1 (MQ=255) agcTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCttt > 1:448526/1‑151 (MQ=255) cGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAg < 1:622287/151‑1 (MQ=255) cGCTCC‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACAACAATGTGATGCGTTTCCGCCAGCAGTTCCAGAGACAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTAt < 1:388305/147‑1 (MQ=255) ctct‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCg > 1:314608/1‑151 (MQ=255) ctct‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCg > 1:195649/1‑151 (MQ=255) ct‑cCATCTTCTGAACAACCGGTGCCACAAACGTGTTTACATCAGTGTGTTGGTTTTTCGCCAGCAGTCCCAGATGAAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAg < 2:835382/147‑1 (MQ=255) ct‑cCATCATCTGATCAAGCCGGGCCACTAACGGTGCTACATAAATGTGATGCTTTTCCGCCAGCAGTCCCAGATGAAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGt < 1:463284/150‑1 (MQ=255) ccATCATCTGATCAAGCCGTGCCACTAACGGTACTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGc > 1:956814/1‑150 (MQ=255) cgtcTGATCAGCCCTGGCCACTACGGGGCCTACACAAATGTGAGCCGTTTCGGCCGGCATTCCCGGGGGAAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTgg < 2:427077/147‑1 (MQ=255) tGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAgg > 2:964650/1‑150 (MQ=255) gATCAGGCCTTGCCACTACCGGTGCTACTTCAATTTGTGGCGTTTTCGCCAGCTGTTCCAGACCCAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGc < 2:1008214/150‑1 (MQ=255) cAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACt > 1:861496/1‑151 (MQ=255) aaGCCGTGCCACTACCGGTGCTACATCAAGGTGATGCTTTTTCGCCAGCAGTTCCAGATGCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACt < 1:727637/150‑1 (MQ=255) aaGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACt < 1:350105/150‑1 (MQ=255) aaGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTg > 1:783215/1‑151 (MQ=255) ccACTAACGGTGCAACATCAAGGGGATGCGTTTTCGCCAGCAGTCCGAGAGGCAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATc < 2:612269/151‑1 (MQ=255) cTAACGGTTCTACAACAATGTGAGGCGTTTTCCCCACCAGTACCAAATACAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGt < 1:615091/150‑1 (MQ=255) aaCGTTGCTACATAAATGTGATGCTTTTCCGCCAGCAGTCCAAGATGAAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTa < 1:434380/151‑1 (MQ=255) cGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAAc > 2:297394/1‑151 (MQ=255) ggTGCTACATCAATGTGATGCTTTTTCGCCAGCAGTTCCAGAGCCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACg < 1:1045673/151‑1 (MQ=255) gTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGc > 2:1031408/1‑151 (MQ=255) tACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGa > 1:921254/1‑150 (MQ=255) tgtgATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGc > 1:659001/1‑151 (MQ=255) tGCGTTTCCGCCAGCAGTTCCAGAGGAAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCg < 1:73041/150‑1 (MQ=255) ccAGCATTCCCAGATGAAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGa < 1:1038138/147‑1 (MQ=255) ccAGCAGTTCCAGATCCAAACGCACCCCCGCCAGTGGCGTTCGCAGTACGTGCGCGACGTCAGCGGTAAACAACCTCTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCATATCTTTCACCACCCCAACCACCGCTTCGactcacaac > 2:855888/1‑141 (MQ=255) agaagTTCAAGAGGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAggg < 2:734101/145‑1 (MQ=255) agTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGgcgc > 2:357496/1‑151 (MQ=255) gTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCt > 1:845621/1‑151 (MQ=255) ttCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCtg > 2:667482/1‑151 (MQ=255) ccAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCtgtg > 1:423140/1‑151 (MQ=255) cAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCtgtg > 1:49628/1‑150 (MQ=255) aGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGa > 2:194592/1‑150 (MQ=255) ctgcagccGCCCCGCCGCCAGTGGCGTTCGAAGTTCGTGCGCGACGTCAGAGGTAAACAGCCTTTCGTGAGCCAGCGTGCTGGTTGGGCGTCTGACCAGATCGTTTAACGCCGAATCCACCGCTTCGATTTCGAGTGTTGCGCtgtg < 2:28706/140‑1 (MQ=255) tGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATgg > 1:425079/1‑151 (MQ=255) gCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGc > 2:773571/1‑151 (MQ=255) aaccGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGa < 1:918716/141‑1 (MQ=255) aaccGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGc < 1:37674/146‑1 (MQ=255) | ACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCT‑CCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGC > NC_000913/4332641‑4332933 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |