Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,555,109 | 0 | T | A | 37.7% | 79.3 / 15.1 | 54 | N178K (AAT→AAA) | uxuR | fructuronate‑inducible hexuronate regulon transcriptional repressor; autorepressor |
Reads supporting (aligned to +/- strand): ref base T (11/22); new base A (20/0); total (32/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.35e-07 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTA > NC_000913/4554963‑4555257 | cAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAAcc > 2:466873/1‑149 (MQ=255) aaCTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAAt < 1:75153/151‑1 (MQ=255) aCTGGAAGAGCGTTAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAAtg < 2:449442/151‑1 (MQ=255) gCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATG‑TGGATcc > 1:775678/1‑149 (MQ=255) tGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATG‑TGGATCCAGtt > 1:533837/1‑150 (MQ=255) gAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGGTCCAGtt > 1:227388/1‑149 (MQ=255) ggCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGcaca < 1:535377/149‑1 (MQ=255) ttCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGGTCCCGTTGCCCAGcc > 1:433368/1‑149 (MQ=255) tCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCGGGCAGTGGCGGGAAAACAATCCAATG‑TGGAGCCAGTTGCACAGCCAt < 2:631387/150‑1 (MQ=255) cgcCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAAAG‑TGGGGCCAGTTGCAACGCCATCTgggt > 2:261410/1‑147 (MQ=255) gcCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCCATG‑TGGGTCCAGTTGCACAGCCATCTGGGTg > 1:802757/1‑149 (MQ=255) agcagcGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGt < 1:835966/151‑1 (MQ=255) agcGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATc < 1:363278/151‑1 (MQ=255) agcGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAATG‑TGGATCCAGTTGCACAGCCCACTGGGTGAAAGccacta > 2:752374/1‑145 (MQ=255) aaaGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCaa < 1:827952/151‑1 (MQ=255) aaaGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCCAAG‑TGGGGCCCGTTTCCCAACCCTCTGGATGAAAGACCGTATAGc > 2:576061/1‑149 (MQ=255) tGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGGTGACAGCCTGTATCGCAAAGAGTgg > 1:137136/1‑151 (MQ=255) ttCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAACCAATG‑TGGGTCCCGGTTCACAGCCATCTGGGTGACAGCCCGTATCGCCAAGAGGGGGTGGGGGATc > 1:449853/1‑151 (MQ=255) ttCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAAACAACA‑GGGATCCTGTTGCACCGCCACATGGAGGAAATCCTGTGTCCGACAGAGAGGGTGGGCgggt > 2:651384/1‑148 (MQ=255) cGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACaaa < 1:869849/148‑1 (MQ=255) cGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACa < 1:883297/150‑1 (MQ=255) cGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGAGCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACa < 2:816392/150‑1 (MQ=255) aTTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGAt < 2:991244/149‑1 (MQ=255) ttGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAAACAAAG‑TGGGTACCGGTGGAAAGCCATCTGGGGGACAGCCCGTATCGCAAAAAGAGGTTGGGGGGTCACcaaaaagacc > 2:943962/1‑143 (MQ=255) tGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAACCCAAAG‑TGGGGCCAGTTGCAAAGCCATCTGGATGAAAGCCTGTATCGCCACAAGTGGGTGGGGGAACACAAACAGATCCt > 2:686798/1‑150 (MQ=255) gCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATG‑TGGGTCCCGGTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGGGGGTGGGGGATCACAAAAAGAAcccc > 1:642180/1‑148 (MQ=255) ccGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATG‑TGGGTCCAGTTGCACAGCCCTCTGGGTGACAGCCTGTATTGCAAAGAGGGGGTGGGCGATCACAAAAAGAAcc > 1:686929/1‑147 (MQ=255) ccGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATG‑GGGGTCCAGGTGCACCGCCAACTGGGTGACAGCCTGTATCCCAAAGAGGGGGTGGGGGGTCACAAAAAGAACCTc > 2:197879/1‑149 (MQ=255) cGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATG‑TGGGTCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGGGGTTGGGCGATCACAAAAAGATCCTcgcc > 1:1005439/1‑151 (MQ=255) gAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATG‑TGGGTCCAGGTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGGGGGTGGGCGATCACAAACAGATCCTcgcc > 1:293925/1‑150 (MQ=255) aaGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTcgccgc < 1:445365/151‑1 (MQ=255) aaGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAACCAATG‑TGGATCCAGTTTCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGGGGGTTGGCGATCACAAAAAGAACCCcgcc > 1:311541/1‑149 (MQ=255) gCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGt < 1:48867/151‑1 (MQ=255) gCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATG‑GGGGTCCAGTTGCACAGCCATCTGGATGGAAGCCTGTATCGCCAAGAGAGGTTGGGGGATCACAAACAGAACCTc > 2:920637/1‑144 (MQ=255) aaCAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGGTCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGGGGGTGGGGGGTCACAAACAAATCCTCGCCGCGGTAATCaaa > 1:472830/1‑150 (MQ=255) aGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCGATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAg < 1:997331/151‑1 (MQ=255) aGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAAAGTTGGGCCCAATT‑CTCAGCCCTCTTGGTGACAGCCTCTATTCTAAAAAGAGGGTGGGCGACTACAAAACGACACTCGCCGCCCTAAACaaaaaa > 2:856397/1‑150 (MQ=255) cATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGAt < 1:622350/151‑1 (MQ=255) gCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAACCAATG‑TGGGTCCAGGTGCACAGGCATCTGGGTGGCAACCTGTAACGCCAAAAGGGGGTGGGGGGTCACAAAAAGAACCTCCCCCCCGTAAATAAAAAAAATGc > 2:403313/1‑150 (MQ=255) ggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAACAAACCAATG‑TGGATCCCGGTGCCCAGCCATCTGGGTGACAGCATGTATCGCAAAGAGAGGGTGGGGGGGCACAAAAAGAACCTCGCCGCGGTTATCAAAAAAAATGCCCGAggg > 2:367237/1‑149 (MQ=255) ggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAgcg < 2:939423/151‑1 (MQ=255) ggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCAACTGGATAACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCTCGTTAATCAAAAAATAGGCCCGAgcg < 2:894025/151‑1 (MQ=255) ggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAATG‑TGGGTCCAGTTGCACAGCCATCTGGGTGACAGCCTGTATCGCAAAGAGTGGGTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAgcg > 1:579343/1‑151 (MQ=255) cTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAACCTGTATCGCAAAGAGGGGTTGGGCGATCACAAACAGAACCTCGCCGCGTTAATCAAAAAAAAAGCCCGAggggcg > 1:434921/1‑150 (MQ=255) tGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAACCCATG‑TGGATCCCGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGGTGGGCGGTCACAAAAAGAACCTCGCCCCGGTAATCAAAAAAAAAGCCCGAgggg > 1:157071/1‑147 (MQ=255) gTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGa < 2:449386/149‑1 (MQ=255) ccGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTg < 2:936329/151‑1 (MQ=255) gTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAATG‑TGGGTCCAGGGGCACAGCCATCTTGAAGGCAACCCGTATCGCAAAGGGGGGGTGGGGGAACACAAACAGATCCCCGCCGCGGTAAACAAAAAAAAGTACCGAGCGGAGAAGCggg > 1:210822/1‑150 (MQ=255) ggCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTgg < 2:136874/149‑1 (MQ=255) gCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTg < 1:896296/151‑1 (MQ=255) gAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGaa > 1:487970/1‑150 (MQ=255) aaaCAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAAc > 1:81024/1‑151 (MQ=255) cAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGtt < 2:432910/151‑1 (MQ=255) aaTCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGtt > 2:317215/1‑150 (MQ=255) tCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTa > 1:430402/1‑149 (MQ=255) | CAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATG‑TGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTA > NC_000913/4554963‑4555257 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |