Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,555,1090TA37.7% 79.3 / 15.1 54N178K (AAT→AAAuxuRfructuronate‑inducible hexuronate regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (11/22);  new base A (20/0);  total (32/22)
Fisher's exact test for biased strand distribution p-value = 4.35e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTA  >  NC_000913/4554963‑4555257
                                                                                                                                                  |                                                                                                                                                     
cAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAAcc                                                                                                                                                     >  2:466873/1‑149 (MQ=255)
 aaCTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAAt                                                                                                                                                  <  1:75153/151‑1 (MQ=255)
  aCTGGAAGAGCGTTAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAAtg                                                                                                                                                 <  2:449442/151‑1 (MQ=255)
           gCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATGTGGATcc                                                                                                                                         >  1:775678/1‑149 (MQ=255)
              tGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATGTGGATCCAGtt                                                                                                                                     >  1:533837/1‑150 (MQ=255)
               gAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGGTCCAGtt                                                                                                                                     >  1:227388/1‑149 (MQ=255)
                    ggCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGcaca                                                                                                                                <  1:535377/149‑1 (MQ=255)
                       ttCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGGTCCCGTTGCCCAGcc                                                                                                                             >  1:433368/1‑149 (MQ=255)
                        tCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCGGGCAGTGGCGGGAAAACAATCCAATGTGGAGCCAGTTGCACAGCCAt                                                                                                                           <  2:631387/150‑1 (MQ=255)
                               cgcCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAAAGTGGGGCCAGTTGCAACGCCATCTgggt                                                                                                                     >  2:261410/1‑147 (MQ=255)
                                gcCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCCATGTGGGTCCAGTTGCACAGCCATCTGGGTg                                                                                                                    >  1:802757/1‑149 (MQ=255)
                                       agcagcGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGt                                                                                                           <  1:835966/151‑1 (MQ=255)
                                          agcGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATc                                                                                                        <  1:363278/151‑1 (MQ=255)
                                          agcGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAATGTGGATCCAGTTGCACAGCCCACTGGGTGAAAGccacta                                                                                                          >  2:752374/1‑145 (MQ=255)
                                              aaaGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCaa                                                                                                    <  1:827952/151‑1 (MQ=255)
                                              aaaGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCCAAGTGGGGCCCGTTTCCCAACCCTCTGGATGAAAGACCGTATAGc                                                                                                      >  2:576061/1‑149 (MQ=255)
                                                     tGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGGTGACAGCCTGTATCGCAAAGAGTgg                                                                                             >  1:137136/1‑151 (MQ=255)
                                                               ttCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAACCAATGTGGGTCCCGGTTCACAGCCATCTGGGTGACAGCCCGTATCGCCAAGAGGGGGTGGGGGATc                                                                                   >  1:449853/1‑151 (MQ=255)
                                                               ttCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAAACAACAGGGATCCTGTTGCACCGCCACATGGAGGAAATCCTGTGTCCGACAGAGAGGGTGGGCgggt                                                                                   >  2:651384/1‑148 (MQ=255)
                                                                       cGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACaaa                                                                              <  1:869849/148‑1 (MQ=255)
                                                                       cGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACa                                                                            <  1:883297/150‑1 (MQ=255)
                                                                       cGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAGCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACa                                                                            <  2:816392/150‑1 (MQ=255)
                                                                           aTTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGAt                                                                         <  2:991244/149‑1 (MQ=255)
                                                                            ttGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAAACAAAGTGGGTACCGGTGGAAAGCCATCTGGGGGACAGCCCGTATCGCAAAAAGAGGTTGGGGGGTCACcaaaaagacc                                                                       >  2:943962/1‑143 (MQ=255)
                                                                             tGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAACCCAAAGTGGGGCCAGTTGCAAAGCCATCTGGATGAAAGCCTGTATCGCCACAAGTGGGTGGGGGAACACAAACAGATCCt                                                                      >  2:686798/1‑150 (MQ=255)
                                                                              gCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATGTGGGTCCCGGTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGGGGGTGGGGGATCACAAAAAGAAcccc                                                                     >  1:642180/1‑148 (MQ=255)
                                                                               ccGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATGTGGGTCCAGTTGCACAGCCCTCTGGGTGACAGCCTGTATTGCAAAGAGGGGGTGGGCGATCACAAAAAGAAcc                                                                       >  1:686929/1‑147 (MQ=255)
                                                                               ccGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATGGGGGTCCAGGTGCACCGCCAACTGGGTGACAGCCTGTATCCCAAAGAGGGGGTGGGGGGTCACAAAAAGAACCTc                                                                     >  2:197879/1‑149 (MQ=255)
                                                                                cGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATGTGGGTCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGGGGTTGGGCGATCACAAAAAGATCCTcgcc                                                                  >  1:1005439/1‑151 (MQ=255)
                                                                                 gAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATGTGGGTCCAGGTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGGGGGTGGGCGATCACAAACAGATCCTcgcc                                                                  >  1:293925/1‑150 (MQ=255)
                                                                                  aaGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTcgccgc                                                                <  1:445365/151‑1 (MQ=255)
                                                                                  aaGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAACCAATGTGGATCCAGTTTCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGGGGGTTGGCGATCACAAAAAGAACCCcgcc                                                                  >  1:311541/1‑149 (MQ=255)
                                                                                    gCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGt                                                              <  1:48867/151‑1 (MQ=255)
                                                                                    gCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATGGGGGTCCAGTTGCACAGCCATCTGGATGGAAGCCTGTATCGCCAAGAGAGGTTGGGGGATCACAAACAGAACCTc                                                                     >  2:920637/1‑144 (MQ=255)
                                                                                             aaCAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGGTCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGGGGGTGGGGGGTCACAAACAAATCCTCGCCGCGGTAATCaaa                                                      >  1:472830/1‑150 (MQ=255)
                                                                                                aGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCGATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAg                                                  <  1:997331/151‑1 (MQ=255)
                                                                                                aGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAAAGTTGGGCCCAATTCTCAGCCCTCTTGGTGACAGCCTCTATTCTAAAAAGAGGGTGGGCGACTACAAAACGACACTCGCCGCCCTAAACaaaaaa                                                   >  2:856397/1‑150 (MQ=255)
                                                                                                  cATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGAt                                                <  1:622350/151‑1 (MQ=255)
                                                                                                     gCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAACCAATGTGGGTCCAGGTGCACAGGCATCTGGGTGGCAACCTGTAACGCCAAAAGGGGGTGGGGGGTCACAAAAAGAACCTCCCCCCCGTAAATAAAAAAAATGc                                              >  2:403313/1‑150 (MQ=255)
                                                                                                           ggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAACAAACCAATGTGGATCCCGGTGCCCAGCCATCTGGGTGACAGCATGTATCGCAAAGAGAGGGTGGGGGGGCACAAAAAGAACCTCGCCGCGGTTATCAAAAAAAATGCCCGAggg                                       >  2:367237/1‑149 (MQ=255)
                                                                                                           ggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAgcg                                       <  2:939423/151‑1 (MQ=255)
                                                                                                           ggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCAACTGGATAACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCTCGTTAATCAAAAAATAGGCCCGAgcg                                       <  2:894025/151‑1 (MQ=255)
                                                                                                           ggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAATGTGGGTCCAGTTGCACAGCCATCTGGGTGACAGCCTGTATCGCAAAGAGTGGGTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAgcg                                       >  1:579343/1‑151 (MQ=255)
                                                                                                               cTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAACCTGTATCGCAAAGAGGGGTTGGGCGATCACAAACAGAACCTCGCCGCGTTAATCAAAAAAAAAGCCCGAggggcg                                    >  1:434921/1‑150 (MQ=255)
                                                                                                                tGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAACCCATGTGGATCCCGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGGTGGGCGGTCACAAAAAGAACCTCGCCCCGGTAATCAAAAAAAAAGCCCGAgggg                                      >  1:157071/1‑147 (MQ=255)
                                                                                                                 gTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGa                                   <  2:449386/149‑1 (MQ=255)
                                                                                                                    ccGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTg                              <  2:936329/151‑1 (MQ=255)
                                                                                                                      gTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAATGTGGGTCCAGGGGCACAGCCATCTTGAAGGCAACCCGTATCGCAAAGGGGGGGTGGGGGAACACAAACAGATCCCCGCCGCGGTAAACAAAAAAAAGTACCGAGCGGAGAAGCggg                             >  1:210822/1‑150 (MQ=255)
                                                                                                                               ggCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTgg                     <  2:136874/149‑1 (MQ=255)
                                                                                                                                      gCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTg            <  1:896296/151‑1 (MQ=255)
                                                                                                                                          gAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGaa         >  1:487970/1‑150 (MQ=255)
                                                                                                                                            aaaCAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAAc      >  1:81024/1‑151 (MQ=255)
                                                                                                                                               cAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGtt   <  2:432910/151‑1 (MQ=255)
                                                                                                                                                aaTCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGtt   >  2:317215/1‑150 (MQ=255)
                                                                                                                                                  tCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTa  >  1:430402/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTA  >  NC_000913/4554963‑4555257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: