Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,397,830	0	T	C	41.7%	69.3 / 18.4	61	I265V (ATT→GTT)	uspE	stress‑induced protein
Reads supporting (aligned to +/- strand):  ref base T (24/11);  new base C (0/25);  total (24/37)
Fisher's exact test for biased strand distribution p-value = 1.39e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTT  >  NC_000913/1397682‑1397971                                                                                                                                                     |                                                                                                                                              tcttcGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAAt                                                                                                                                               <  1:840405/149‑1 (MQ=255) tcttcGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAAt                                                                                                                                               <  1:337084/149‑1 (MQ=255)    tcGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAAGccc                                                                                                                                            <  2:652409/149‑1 (MQ=255)     cGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGc                                                                                                                                         >  2:1044013/1‑151 (MQ=255)                 cAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTc                                                                                                                             >  1:261985/1‑151 (MQ=255)                           cTGATTCTGGTCGGTTTTAATGCCCGCCAGGCCGCAGGGAAGATGAACAATCCCCTGTCCCGCCGTGTTGCCGAGGAAGGCGGCTAAAATCCCGGGGCGCCCGCCCGTGCCCAGAACCACAACCCCCGCCTGTAAGTGCTCCGCCaa                                                                                                                       <  1:479885/147‑1 (MQ=255)                           cTGATACTGGTCAGGTTTAATAACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATc                                                                                                                    >  1:694165/1‑150 (MQ=255)                              aTACTGGTCAGGTTTAATGACCGGCAGGTCGCAGCGAAGATGATCAATCCCCTGTCCCGCCGTGTTGCCGAGAAATGCTGCTAAAATCCCGGTGCGCCCTACCGTGCCCAGAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCa                                                                                                                   <  1:432204/148‑1 (MQ=255)                                        ggTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtctt                                                                                                      >  1:769601/1‑151 (MQ=255)                                        ggTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGCGCGTCCTACCGTGCCCAGCACCACAATCCCCGCCTGTACGTGCTCCGCCAAATCAGGAATCACctct                                                                                                       >  2:335515/1‑150 (MQ=255)                                         gTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtcttc                                                                                                     >  2:884707/1‑151 (MQ=255)                                         gTTTAACGCCCAGCAGGTCGCAGCGAAGATGACCATTCCCTTTGCCCCCCGTTTTCCCGGGGAGTCCTGCTGAAAGCCCGGTCTGCCCTCCCGGCCCCAAAACCACACTCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACct                                                                                                         <  2:585516/147‑1 (MQ=255)                                           ttAATGACCAGCAGGCCGCAGCAAAGAGAACCAATCCCCTGTCCCCCCGGTTTCCCGAGAAATCCGCCTGAAATCCCGGTGCGTCCTACCGGGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtcttct                                                                                                    <  2:527382/150‑1 (MQ=255)                                             aaTGCCAAGCAGGTCGCAGCGAAGATGACAAATCCCCTGTCCCCCCGTTTTGCCGAGAAAGGCTGCTAAAATCCCGGGGCTCCCTACCGTGCCCAGAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTg                                                                                                   <  2:1008771/149‑1 (MQ=255)                                             aaTGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGc                                                                                                 >  2:866118/1‑151 (MQ=255)                                                  ccAGCAGGTCGCAGCGAAGATGATCAATCCCCTGTCCCGCCGTGTTGCCGAGGAATGCTGCTAAAATCCCGGTGCGCCCTACCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGa                                                                                              <  1:869134/149‑1 (MQ=255)                                                  ccAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGAcc                                                                                            >  1:557550/1‑151 (MQ=255)                                                  ccAGCAGGTCGCAGCAAAGAGAATAAACCCCCTGTTCCGCGGTGTTGCCGAGGAATGCTGCTAAAATACCGGGGCGTCCTACCGTGCCCAGACCCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAg                                                                                               <  1:148100/148‑1 (MQ=255)                                                      cagGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCtttt                                                                                        >  1:914786/1‑151 (MQ=255)                                                      cagGTCGCAGCGAAGATGACAAATCACCTGTCCCGCCGTGTTGCCGAGGAATGCTGCTGAAATCCCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCtt                                                                                          <  1:103087/149‑1 (MQ=255)                                                      cagGCCGCGGGGAAGATGATCAATCCCCTGTTCCGCGGTGTGGCCGAGGAATGCTACTGAAATACCGGTGCGCCCTACCGTTCCCAGACCCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCttt                                                                                         <  1:354817/150‑1 (MQ=255)                                                        ggTGGCGGCGAAGATGATCACTCCCCTGTCCCGCGGTGTTGCCGAGGATTCCTGCTAAAATCCGGGTGCGCCCTCCCGCGCCCAAACCCAAACCCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCtttt                                                                                        <  2:947524/149‑1 (MQ=255)                                                        ggTCGCAGCGAAGATGATCAATCCCCTTTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATCCCGGTGCGCCCTCCCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCtttt                                                                                        <  1:961213/149‑1 (MQ=255)                                                        ggTCGCAGCGAAGATGATCAATCCCCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTAAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCt                                                                                           <  1:899416/146‑1 (MQ=255)                                                            gcagaaaaattgaaCAATCCCCTGTTCCCCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTCCCGTCCCCAAACCCACACCCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCt                                                                                     <  2:719583/138‑1 (MQ=255)                                                            gCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCGGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGTATCACCTCTTCTGGCAGACCTTTTTCtcc                                                                                   >  2:188061/1‑148 (MQ=255)                                                              aGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGt                                                                                 >  2:351262/1‑150 (MQ=255)                                                              aGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTg                                                                                >  2:561463/1‑151 (MQ=255)                                                               gCGAAGATGATCAATCCCCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATCCCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTg                                                                                <  1:882136/150‑1 (MQ=255)                                                                 gAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGt                                                                             >  1:342213/1‑151 (MQ=255)                                                                  aggatgatCAACCCCCTTTCCCGCCGTTTGGCGGAGGAATGCTGCTGAAATCCCGGGGCGCCCTACCGTCCCCAGAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTc                                                                            <  2:502859/149‑1 (MQ=255)                                                                  aaGATGATCAATCACCTGTTCCGCCGTGTTGCGGAGGAATGCTGCTAAAATACCGGTGCGTCCTCCCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTc                                                                            <  1:985620/151‑1 (MQ=255)                                                                  aaGATGATCAATCACCTGTCCCGCCGTGTTGCCGAGGAATGCTGCTGAAATCCCGGTGCGTCCTACCGTGCCCAGAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGt                                                                             <  1:209967/150‑1 (MQ=255)                                                                          caatcaCCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGCGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTc                                                                    >  1:734512/1‑151 (MQ=255)                                                                          caatcaCCTGTTCCGCCGTGTTGACGAGGAATGCTGCTGAAATACCGGCGCGTCCTACCGTGCCCAGAACCACAATCCCCGACTGAAAGTGCTCAGCCAAATCAGGAATCACCTCTTCTGGCAGACGTTTTTCTACAAGCGTCATGTTTTc                                                                    >  2:494738/1‑151 (MQ=255)                                                                              caCCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATCCCGGTGCGTCCTACCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCAtt                                                                 <  1:885309/150‑1 (MQ=255)                                                                              caCCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCAtt                                                                 >  1:934010/1‑150 (MQ=255)                                                                                ccTGTCCCGCCGTGTTGCCGAGGAATGCTGCTGAAATCCCGGTGCGTCCTACCGTGCCAAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAAt                                                              <  2:966383/151‑1 (MQ=255)                                                                                    tcccgccgTGTTGCCGAGGAATGCTGCTAAAATCCCGGTGCGTCCTACCGTGCCCAGAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGc                                                            <  1:26487/147‑1 (MQ=255)                                                                                     cccgccgTGTTGCCGGGAAATGCTGCTAAAATACCGGGGCGTCCTACCGTGCCCAGAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGa                                                         <  1:88055/150‑1 (MQ=255)                                                                                      ccgctgTTTTGCCGAGAAATGCTGCTGAAATACCGGTGCGTCCTCCCGTCCCCAGACCCACATCCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCg                                                          <  2:552966/149‑1 (MQ=255)                                                                                       cgccgTGTTGCGGAGGAATGCTGCTAAAATCCCGGTGCGTCCTCCCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAAt                                                       <  1:451686/151‑1 (MQ=255)                                                                                       cgccgTGTTGCCGAGGAATGCTGCTGAAATCCCGGGGCGTCCTACCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAAt                                                       <  2:349882/151‑1 (MQ=255)                                                                                        gccgTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAAtt                                                      >  2:1037978/1‑151 (MQ=255)                                                                                         cagtgtAGCCGAGGAATGCTGCTGAATTCCCGGTGCGTCCTGCCGTCCAAACACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAAttt                                                     <  1:889969/149‑1 (MQ=255)                                                                                              ttGCGGAGAAATGCTGCTAAAATCCCGGTGCGTCCTACCGTCCCCAGACCCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTg                                                  <  1:1044667/149‑1 (MQ=255)                                                                                              ttGCCGAGGAATGCTGCTGAAATCCCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGc                                                 <  1:999338/150‑1 (MQ=255)                                                                                                  caaGGAATGCTCCTAAAATACCGGTGCGTCCTACCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGcc                                                <  2:693615/145‑1 (MQ=255)                                                                                                   gAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAgg                                           >  2:756377/1‑151 (MQ=255)                                                                                                    aGGAATGCTTCGGAAATCCCGGTGCGCCCTACCGTCCCCAAAACCCCAACCACCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCa                                             <  2:451877/148‑1 (MQ=255)                                                                                                     ggAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGACCCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGc                                         <  1:150489/151‑1 (MQ=255)                                                                                                     ggAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAggg                                          <  1:561963/150‑1 (MQ=255)                                                                                                       actgctgcTGAAATACCGGTGCGTCCTACCGTGCCCCGCACCACAATCCCCGCCTGTAAGTGCTCCGCCACATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGACGCAGGGCtt                                       >  2:688718/3‑151 (MQ=255)                                                                                                       aaTGCTGCTGAAATCCCGGTGCGTCCTCCCGTGCCCAAACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCtt                                       <  1:667526/151‑1 (MQ=255)                                                                                                                gaaaccccgttccGTCCTCCGGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCGTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCa                                    <  2:112081/133‑1 (MQ=255)                                                                                                                  aaTCCCGGTGCGTCCTACCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGc                            <  1:426123/151‑1 (MQ=255)                                                                                                                      ccGGTGCGGCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGCATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCa                              >  2:212666/1‑145 (MQ=255)                                                                                                                               ccTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCACATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCAc               >  2:1041046/1‑151 (MQ=255)                                                                                                                                     gTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATgg         <  1:697508/151‑1 (MQ=255)                                                                                                                                        cccAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATgg         <  2:557051/148‑1 (MQ=255)                                                                                                                                           aGAACCACAATTCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGt   >  1:486194/1‑151 (MQ=255)                                                                                                                                            gAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGtt  >  1:1039111/1‑151 (MQ=255)                                                                                                                                             aaCCACAATCTCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGtt  >  2:471702/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                              TCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTT  >  NC_000913/1397682‑1397971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑