Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,824,137	G→T	D580Y (GAC→TAC)	spoT →	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,824,137	0	G	T	100.0%	246.9 / NA	67	D580Y (GAC→TAC)	spoT	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (35/32);  total (35/32)

AACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGA  >  NC_000913/3823990‑3824282                                                                                                                                                    |                                                                                                                                                  aaCGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTAcc                                                                                                                                                >  2:1046013/1‑151 (MQ=255)    gCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTACAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCg                                                                                                                                              >  2:485419/1‑150 (MQ=255)      aaTGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGa                                                                                                                                             <  2:217611/149‑1 (MQ=255)          aGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCg                                                                                                                                       >  1:457089/1‑151 (MQ=255)           gCGTGGTGGTCGCGAAAAAGCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGc                                                                                                                                      <  2:858827/151‑1 (MQ=255)            cGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGcc                                                                                                                                     >  1:268270/1‑151 (MQ=255)             gtggtgGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGccc                                                                                                                                    >  1:946068/1‑151 (MQ=255)                  ggtCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGt                                                                                                                                <  1:472002/150‑1 (MQ=255)                  ggtCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTc                                                                                                                               >  1:486670/1‑151 (MQ=255)                         aaaaaTCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCg                                                                                                                        >  1:434034/1‑151 (MQ=255)                            aaTCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGt                                                                                                                      >  2:799608/1‑150 (MQ=255)                                  cAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTc                                                                                                               <  1:760283/151‑1 (MQ=255)                                          ggACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATc                                                                                                       <  2:236093/151‑1 (MQ=255)                                           gACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATcc                                                                                                      <  1:686789/151‑1 (MQ=255)                                                    aTTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAAt                                                                                             >  2:984113/1‑151 (MQ=255)                                                     ttCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATc                                                                                            >  2:557326/1‑151 (MQ=255)                                                        cACCGGCAACCCAAAGCCACGGACATCTGCCGATTAAAGGCGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCCGGCTACCCGATTATCGCCCACGTCAGCCCCGGCCAAGGTCTGGTGATCCACCATGAATCCTg                                                                                         >  2:467904/1‑151 (MQ=255)                                                           cGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCg                                                                                      >  1:959419/1‑151 (MQ=255)                                                                       gCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGCATCCTGCCGTAATATCCGTgg                                                                          >  2:714262/1‑151 (MQ=255)                                                                         cACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCt                                                                        <  2:517543/151‑1 (MQ=255)                                                                           cGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTAc                                                                      >  1:142168/1‑151 (MQ=255)                                                                                aTCTGCCCATTAAAGGTGCCGAGGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGa                                                                  <  2:10762/150‑1 (MQ=255)                                                                                   tGCCCATAAAAGGTGCCGATGGCGGGCTGATCACCTTTGCGAAATCCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCTCCATGAATCCTTCCGTAATATCCGTGGCTACCa                                                                    <  2:233651/145‑1 (MQ=255)                                                                                    gCCCATTAAAGGTGCCGATGGCGTGCTGATCCCCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGaa                                                                 <  1:285166/147‑1 (MQ=255)                                                                                    gCCCATTAAAGGTGCCGATGGCGTGCTGATCCCCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAagag                                                             <  1:110938/151‑1 (MQ=255)                                                                                     cccATTAAAGGTGCCGATGGGGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGGGATCCGCCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGc                                                            <  2:546877/151‑1 (MQ=255)                                                                                      ccATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGcc                                                           <  2:357415/151‑1 (MQ=255)                                                                                      ccATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGcc                                                           >  1:128424/1‑151 (MQ=255)                                                                                       cATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGcc                                                           >  1:296920/1‑150 (MQ=255)                                                                                       cATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCa                                                          <  1:622231/151‑1 (MQ=255)                                                                                          tAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCag                                                         >  1:626476/1‑149 (MQ=255)                                                                                             aGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAgaga                                                      >  1:490312/1‑149 (MQ=255)                                                                                               gTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGtt                                                  <  1:269104/151‑1 (MQ=255)                                                                                               gTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGtt                                                  <  1:1046670/151‑1 (MQ=255)                                                                                                  ccGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTAt                                               <  2:256651/151‑1 (MQ=255)                                                                                                  ccGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTAt                                               <  1:1020600/151‑1 (MQ=255)                                                                                                    gATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATgg                                             <  1:352351/151‑1 (MQ=255)                                                                                                    gATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATgg                                             >  1:706225/1‑151 (MQ=255)                                                                                                     aTGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGc                                            >  1:279380/1‑151 (MQ=255)                                                                                                     aTGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGc                                            >  1:696713/1‑151 (MQ=255)                                                                                                      tGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCt                                           >  1:315361/1‑151 (MQ=255)                                                                                                       ggCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCt                                           <  1:132934/150‑1 (MQ=255)                                                                                                          gTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTgg                                       >  1:979100/1‑151 (MQ=255)                                                                                                             cTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAAt                                    >  1:309962/1‑151 (MQ=255)                                                                                                              tGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATg                                   >  2:452956/1‑151 (MQ=255)                                                                                                               gATCACCGTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAAGATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAAt                                    <  2:63170/149‑1 (MQ=255)                                                                                                                 tCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATggg                                 >  2:126398/1‑150 (MQ=255)                                                                                                                  cACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCAAGAGAAGTTTATGGCTGTGGAATggg                                 >  2:868272/1‑149 (MQ=255)                                                                                                                    ccTTTGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATaa                             <  1:267074/151‑1 (MQ=255)                                                                                                                        tGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAaga                          <  1:825585/150‑1 (MQ=255)                                                                                                                        tGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAaga                          <  1:853585/150‑1 (MQ=255)                                                                                                                        tGCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAaga                          <  1:356615/150‑1 (MQ=255)                                                                                                                         gCGAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAagag                         <  1:412246/150‑1 (MQ=255)                                                                                                                          cGAAATGCTGCCGCCCTATTCCTGGCTCCCCGATTATCGCCCACGTCAGCCCCGGTGAAGGTCTGGTGCTCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAagag                         <  2:98393/149‑1 (MQ=255)                                                                                                                           gAAATGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACg                      >  1:727961/1‑151 (MQ=255)                                                                                                                             aaTGCTGCCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACgg                     >  2:582193/1‑150 (MQ=255)                                                                                                                               tgctgcCGCCCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGgcgc                  >  1:177284/1‑151 (MQ=255)                                                                                                                               tgctgcCGCCATATTCCTGGCTACCAGATTATCGCCCACGTCAGCCCCGGAAAAGGTCTGGTGCTCCACCATGAATCCTGCCGTAATCTCCGCGGCGACCAGAAATAGCCCGAGAAGTTTATGGCTGCGGAATGGGAAAAAGAGACGgagc                  >  2:305008/1‑151 (MQ=255)                                                                                                                                   gccgccCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAgg               <  2:784927/150‑1 (MQ=255)                                                                                                                                   gccgccCTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAgg               <  1:740223/150‑1 (MQ=255)                                                                                                                                       cccTATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTtc          >  1:540426/1‑151 (MQ=255)                                                                                                                                          tATTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTtcatc       >  1:129147/1‑151 (MQ=255)                                                                                                                                           aTTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTtcatca      <  1:696616/151‑1 (MQ=255)                                                                                                                                           aTTCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTtcat        <  1:82034/149‑1 (MQ=255)                                                                                                                                            ttCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCAc     >  2:819561/1‑151 (MQ=255)                                                                                                                                            ttCCTGGCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCAc     >  1:761886/1‑151 (MQ=255)                                                                                                                                                 ggCTACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGa  <  2:842846/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  AACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGA  >  NC_000913/3823990‑3824282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑