Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,724,611	G→A	A379V (GCG→GTG)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,724,611	0	G	A	100.0%	250.3 / NA	65	A379V (GCG→GTG)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/21);  total (44/21)

TCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGC  >  NC_000913/2724465‑2724757                                                                                                                                                   |                                                                                                                                                   tcccttTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACat                                                                                                                                                 <  1:375459/150‑1 (MQ=255)     tttGCGATGTAACATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGAc                                                                                                                                            >  1:458007/1‑151 (MQ=255)         cGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGAc                                                                                                                                        >  1:70808/1‑151 (MQ=255)           aTGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGAcc                                                                                                                                       >  1:655871/1‑150 (MQ=255)                 cATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCt                                                                                                                                <  2:786398/151‑1 (MQ=255)                       aGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATgcg                                                                                                                          <  2:1008816/151‑1 (MQ=255)                       aGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATgcg                                                                                                                          <  1:748750/151‑1 (MQ=255)                         aaaCCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGcgcg                                                                                                                        >  1:988951/1‑151 (MQ=255)                         aaaCCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGcgcg                                                                                                                        <  1:83131/151‑1 (MQ=255)                         aaaCCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGcgcg                                                                                                                        <  1:642810/151‑1 (MQ=255)                              aGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCt                                                                                                                   <  1:693361/151‑1 (MQ=255)                                aaaCGCCACCACGGCCATCAAGGACACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGACGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCtgt                                                                                                                 >  2:939739/1‑151 (MQ=255)                                   cGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGcc                                                                                                              >  2:779851/1‑151 (MQ=255)                                    gCCACCACGGCCATCAAGGGCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCg                                                                                                             >  2:791980/1‑151 (MQ=255)                                      caccacGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCggg                                                                                                           >  1:441296/1‑151 (MQ=255)                                                  cAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGcc                                                                                               <  1:1047933/151‑1 (MQ=255)                                                   aaGGTCACATACCAGAAGAAGACTGTTTCAATGCCTACTGATTTCAGCGACAACGCTACGTACTCTGCCGAACCACCAACTATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCCCGAACCTGTGCCGGGAACCTCTCa                                                                                                  >  2:912725/1‑147 (MQ=255)                                                        cacaTACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAg                                                                                          <  1:931081/150‑1 (MQ=255)                                                          caTACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTAt                                                                                       >  2:661523/1‑151 (MQ=255)                                                          caTACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTAt                                                                                       >  1:791568/1‑151 (MQ=255)                                                           aTACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTAtt                                                                                      >  1:542088/1‑151 (MQ=255)                                                              ccAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTcc                                                                                    >  2:448682/1‑150 (MQ=255)                                                                agaagaAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACt                                                                                 >  2:924590/1‑151 (MQ=255)                                                                agaagaAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACt                                                                                 <  1:98107/151‑1 (MQ=255)                                                                 gaagaaGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACtg                                                                                <  2:487880/151‑1 (MQ=255)                                                                   agaagGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACtgat                                                                              >  2:251620/1‑151 (MQ=255)                                                                    gaagGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACtgatt                                                                             >  2:939326/1‑151 (MQ=255)                                                                        gCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATg                                                                         >  2:687052/1‑151 (MQ=255)                                                                         cTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAAAATCTCAGCCTTCAGTATTCCACTGATTGATg                                                                         >  2:104933/1‑150 (MQ=255)                                                                           gTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGt                                                                        <  2:273035/149‑1 (MQ=255)                                                                             ttCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGtata                                                                     <  1:725352/150‑1 (MQ=255)                                                                              tCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATaa                                                                    >  1:793136/1‑150 (MQ=255)                                                                                cATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATaaaa                                                                  >  1:661332/1‑150 (MQ=255)                                                                                     cTATTGATTTCAGCGACAACGCTACGTACTCCGCAGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCAc                                                            >  2:126196/1‑151 (MQ=255)                                                                                        ttGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTAt                                                         >  1:56576/1‑151 (MQ=255)                                                                                               cAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATcagcag                                                   >  2:892821/1‑150 (MQ=255)                                                                                                aGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAggg                                                 >  2:911983/1‑151 (MQ=255)                                                                                                aGCGACAACGCAACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATcagcag                                                   >  2:83929/1‑149 (MQ=255)                                                                                                    aCAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcaca                                             <  1:796116/151‑1 (MQ=255)                                                                                                    aCAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcac                                              >  1:109551/1‑150 (MQ=255)                                                                                                    aCAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcac                                              >  2:776035/1‑150 (MQ=255)                                                                                                     cAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcacac                                            >  1:41336/1‑151 (MQ=255)                                                                                                      aaCGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcacac                                            >  2:537425/1‑150 (MQ=255)                                                                                                         gCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCa                                        <  1:133861/151‑1 (MQ=255)                                                                                                            aCGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAGCCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCAAACATCACCa                                     <  2:10933/151‑1 (MQ=255)                                                                                                            aCGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCAcc                                      >  1:75130/1‑150 (MQ=255)                                                                                                               tactacGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGAGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGAc                                  >  2:287465/1‑151 (MQ=255)                                                                                                                aCTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGAcc                                 <  1:251902/151‑1 (MQ=255)                                                                                                                 cTCCGCCGAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGAcc                                 >  1:833216/1‑150 (MQ=255)                                                                                                                    cgccgAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCaaa                              >  1:359253/1‑150 (MQ=255)                                                                                                                     gccgAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCaaa                              >  2:244203/1‑149 (MQ=255)                                                                                                                       ctaaCCACCAAATAGAGCATTAGCGACCACATATGACAGACCAACGCCTAATCCGCGAAGCTGCGCCGGGCACATCCCAGCCTTCAGTATTCCACTGATTGATGGATAAAAACTCAATATCAGCAGGGCACACATCACCAGACCAAAAGc                           >  2:779518/3‑150 (MQ=255)                                                                                                                       cgAACCACCAAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCg                          >  1:386694/1‑151 (MQ=255)                                                                                                                           ccaccaAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCAt                      >  1:1028700/1‑151 (MQ=255)                                                                                                                           ccaccaAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCAt                      >  1:808372/1‑151 (MQ=255)                                                                                                                              ccaAATATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAg                   <  2:216297/151‑1 (MQ=255)                                                                                                                               aaaaTATAGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAgg                  >  2:241510/2‑151 (MQ=255)                                                                                                                                    ataGCATTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAg              <  2:521612/150‑1 (MQ=255)                                                                                                                                        cATTAGCGGCCACACATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCGCACATCGCCAGACCAAAAGCGGCATAAGGCGAGGaaa          <  2:393038/150‑1 (MQ=255)                                                                                                                                         aTTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATACGGCGAGGAAAc         >  2:901270/1‑150 (MQ=255)                                                                                                                                         aTTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACg        >  2:353166/1‑151 (MQ=255)                                                                                                                                         aTTAGCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACg        >  2:981246/1‑151 (MQ=255)                                                                                                                                             gCGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGttt     >  1:352073/1‑150 (MQ=255)                                                                                                                                              cGACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTg   <  1:449149/151‑1 (MQ=255)                                                                                                                                               gACCACATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGc  >  1:527686/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                   TCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGC  >  NC_000913/2724465‑2724757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑