| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 599,576 | 0 | T | G | 50.0% | 44.4 / 17.9 | 40 | V288G (GTG→GGG) | cusA | copper/silver efflux system, membrane component |
| Reads supporting (aligned to +/- strand): ref base T (2/18); new base G (20/0); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.35e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACC > NC_000913/599428‑599725 | aCGCACGAAGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGCGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTTGCTgggggggggg > 1:566239/1‑151 (MQ=255) cGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggtgg > 2:359976/1‑150 (MQ=255) gacgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATcc > 2:215845/1‑151 (MQ=255) acgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATcc > 1:40953/1‑150 (MQ=255) cgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTg < 1:5191/151‑1 (MQ=255) aaTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGggggggggggggg > 1:330265/1‑136 (MQ=255) gTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACg < 2:550793/151‑1 (MQ=255) tAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGccc < 2:122283/150‑1 (MQ=255) aaaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGGCCTGC‑GGTCCGGCaaaaa > 1:258710/1‑144 (MQ=255) tGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGC‑GATCCGGCAAAAAAGCCCGAGAAGTg > 2:544895/1‑148 (MQ=255) gAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATcg < 2:51389/151‑1 (MQ=255) aaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGGGATcgcc > 2:651681/1‑151 (MQ=255) aTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATcgccgc < 2:258766/151‑1 (MQ=255) ggCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCgccgcc < 2:264129/150‑1 (MQ=255) cGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGC‑GATCCGGCAAAAAAGCCCGAGAAGTGATCGCCGCCGTg > 2:701848/1‑151 (MQ=255) ccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAgg < 2:84714/150‑1 (MQ=255) cGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGC‑GATCCGGCAAAAACGCCCGCGAAGGGATCGCCGCCGTGGAgg > 1:242935/1‑149 (MQ=255) aTCTGCGCGATGCTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAAc < 2:496239/150‑1 (MQ=255) gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGC‑GATCCGGCAAAAAAGCCCGGGAAGGGATCGCCGCCGTGGAGGACAAACTgg > 2:194586/1‑149 (MQ=255) cgcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGaa < 2:679870/150‑1 (MQ=255) gcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGC‑GATCCGGCAAAAACGCCCGAGGAGGGATCGCCGCCGCGAAGGACAAACTGGaaa > 1:336238/1‑150 (MQ=255) gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGAAGGACAAACTGGaaa > 2:41838/1‑148 (MQ=255) gCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGaaaa < 2:577282/151‑1 (MQ=255) gacggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGC‑GATTCGGCAAAAAAACGCGGGGAGTGGGCGGCGCCCGGAAGGACAAACTGGGAAAACAGaaaa > 1:72714/4‑149 (MQ=255) gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGACCTAAACGGCGAAGGGGAAGTGGAGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagt < 2:35309/151‑1 (MQ=255) gTCCAGATTGGCCCGGAGATGCGCCGAGGTATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGGGATCGCCGGGGTGAAGGAAAAAATGGaaaa > 2:709214/1‑136 (MQ=255) ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGTGGTTCTGC‑GGTCCCGCCAAAACGGCCGAGAAGTGATCGCCGCCGTGAAGGACAAACATGCAACGATGAAAAGGAGTCTg > 1:526955/1‑151 (MQ=255) ggAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTg < 2:458058/151‑1 (MQ=255) gaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGga < 1:550824/150‑1 (MQ=255) aTGCGCCGGGGCATTGCCGAACTAAGCGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGC‑GATCCCGCAAAAAAGACCGAGAAGGGATCGCCGCCGGGAAGGACAAACTGGAAACGCTGAAAAGAAGTCTGCCGGAAGGCGTgg > 1:110621/1‑148 (MQ=255) gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTaca < 2:418039/151‑1 (MQ=255) aTTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGC‑GACCCGGCAAAAACGCCCGGGGAGGGATCGCCGCCGCGAAGGACAAACTGGAAAAGCTGAAAAGGAGTCTGCCGGAAGGCGGGGGGGTGGTTacaa > 1:280034/1‑148 (MQ=255) ttGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCCCCGGACCCGGC‑CAAACGCCCGAGGAGCGCCAGCCGCCGTGCAGGGCCAACCAGGAACGCTGAAAAGTAGGCTGCCGGACGGAGGGGAGATAGGTccaaca > 1:680729/1‑149 (MQ=255) tGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGC‑GGACCGGCAAAAACGCCCGGGAAGGGAGCGCCGCCGTGAAGGACAAAATGGAAACGCTGAAAAGTAGTCTGCCGGGGGGGGGGGAGATAGTTataaca > 1:48883/1‑148 (MQ=255) tGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGC‑GATCCGGCCAAAACGCCCGAGAAGGGATAGCCGCCCTGAAGGGCAAAATGGAAACCCTGAAAAGAAGTCTGCCGGAAGGGGGGGAGGTAGTTACAACATa > 1:572971/1‑150 (MQ=255) cGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGC‑GATCCGGCCAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACCAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGGGATAGTTACAACATACGa > 2:443443/1‑150 (MQ=255) cGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGGAGGGATCGGCGCCGTGAAGGGCAAACTGGAAAACCTGGAAAGTAGGCTGCCGGGAGGGGGGGGGATAGTTACAACATACGa > 1:213645/1‑150 (MQ=255) gTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgcg < 2:334623/151‑1 (MQ=255) ggcgggcgGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCgcgc < 1:275752/150‑1 (MQ=255) cgggcgggGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCAt > 2:513969/1‑151 (MQ=255) gggcgggGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATc < 2:335346/151‑1 (MQ=255) gggTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACaa < 1:564611/151‑1 (MQ=255) gggTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACa > 1:571596/1‑150 (MQ=255) ggtggtGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAAc > 2:535749/1‑151 (MQ=255) gtggtgATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAAcc < 1:598587/151‑1 (MQ=255) | ACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGC‑GATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACC > NC_000913/599428‑599725 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |