Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4520CA53.1% 0.6 / 27.8 32intergenic (‑196/‑11)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (6/9);  new base A (17/0);  total (23/9)
Fisher's exact test for biased strand distribution p-value = 1.78e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.09e-01

GCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG  >  NC_000913/3206306‑3206600
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gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTgg                                                                                                                                                   <  2:465376/150‑1 (MQ=255)
           ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAAAAAAATAAAAAAAATGGAGTTggc                                                                                                                                            >  1:82732/1‑144 (MQ=255)
             ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAAATTTTTCACAACAAAATAAAAACAGGAg                                                                                                                                                 >  1:322569/1‑139 (MQ=255)
                gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatg                                                                                                                                         <  1:570301/144‑1 (MQ=255)
                 cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAACAAAAACTTGAGGTGCCATGATTAGCg                                                                                                                                  >  2:135662/1‑150 (MQ=255)
                        ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAAAAAAACAAAAAATGGAGTTGGCATGATGAGGggaaggaa                                                                                                                           >  1:474330/1‑150 (MQ=255)
                           attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGAGTTGGCATGGTGAGCggaaggaa                                                                                                                           >  2:180980/1‑147 (MQ=255)
                               ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATAAAAAAAATTGAGTTGCCATGATGAGGGAAAATAATAAACaa                                                                                                                    >  2:639461/1‑150 (MQ=255)
                                 aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAACaa                                                                                                                    >  1:459095/1‑148 (MQ=255)
                                   tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGGTGGCATGATGAGGGAAAATAATAAACAAAAgg                                                                                                                >  2:457858/1‑150 (MQ=255)
                                   tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                <  2:441964/150‑1 (MQ=255)
                                     tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAATAAAAAAAATGGAGGTGCCATGATGAGGGAAAAAAAAAAacaaca                                                                                                                  >  1:520946/1‑146 (MQ=255)
                                         gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAAAACAAAAAAAGGAGGTGTCCTGATGAAGGAAAGTAAAAAGCAACAGGccggg                                                                                                           >  2:601019/1‑145 (MQ=255)
                                              ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACAAAAACAAAAAATGGAGGTGCCATGATGAGGGGAAGGAATAAGAAAAAAGCAGGGAATa                                                                                                       >  2:626021/1‑148 (MQ=255)
                                                 aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGAAAGAAAAAAACAACAAGCAGGGAATAAgat                                                                                                   >  2:477634/1‑147 (MQ=255)
                                                    aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAATACAAAAAACGGGGGTGCCCTGATGAGCGGAAGTAATAAACAACAAGCAGGGAAAAAATTGAc                                                                                                >  2:65621/1‑149 (MQ=255)
                                                      cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGAGTTGCCATGATGAGCGAAAATAATAAAAAACAAGCAGTGAATAAGTTGACa                                                                                               >  2:343773/1‑148 (MQ=255)
                                                       tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCAAACAAATAAAAAAAATAGAGTTTGCATGATGGGCGAAAGTAAAAAAAAAAAAGCAATGAATaaagtgata                                                                                               >  1:58119/1‑140 (MQ=255)
                                                            aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGTGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc                                                                                      <  1:543832/151‑1 (MQ=255)
                                                            aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAAAAAAAAAAATGGAGTTGTCCTGATGAGGGAAAGTAATAAACAACAAGCAGGGAATAAATTGAACGAGATTGtt                                                                                      >  1:225469/1‑150 (MQ=255)
                                                                              cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAATACAAAAAATGGGGTTGGCATGGTGAGCGAAAAGAATAAGAAAAAAGAAGTGAATAAATTGACAGAAAATGTCGCTAAACTTAACGcc                                                                       >  1:614548/1‑148 (MQ=255)
                                                                               aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGGGGTGCCCAGATGAGCGAAAGTAATAAACAACAAGCAGAGAATAAAATGACAGAGAATGTCGCTAACTTTACcccc                                                                       >  2:331614/1‑147 (MQ=255)
                                                                                   aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAAAAAAAAAAAAAACTGGAGTTGGCCTGGTGAGGGAAAGAAATAAACAAAAAGCAGTGGATAAATTGACAGAGATTGTCGCTAACTTTAACGCCATGGtt                                                                 >  1:562040/1‑149 (MQ=255)
                                                                                         aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCg                                                         <  2:521205/151‑1 (MQ=255)
                                                                                              acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACAAAAAAATAAAAAATGGAGTTGCCATGATGGGGGAAAGTAATAAAGAAAAGGCAGGGAATAAATTGAAAGAGAATGTCGCTAAATTTACCGCCATGATTTCTAACCCaa                                                       >  1:704079/1‑148 (MQ=255)
                                                                                               caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATAAAAAAAATGGAGGTGCCCTGATGAGGGGAAGGAATAAACAAAAAGCAGTGAAAAAATTGACAGAGAATGTTGCTAAATTTTCCCCCATTATTTTTAACCCAAAGcc                                                   >  2:238074/1‑151 (MQ=255)
                                                                                                  ttttAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtg                                                 <  1:190412/150‑1 (MQ=255)
                                                                                                              tttGGGGCGGTGAATTTTCCCAGACAAATACAAAAACTGAAGTTGCCATGATGAGCGAAATTAATAAGCAACAGGCAGTGAATATGTTGACAGATATTGGCGTTAACTTGACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                                     <  2:498911/150‑1 (MQ=255)
                                                                                                                                    gACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAt              >  1:547002/1‑151 (MQ=255)
                                                                                                                                       aaaTACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGcc           >  2:376305/1‑151 (MQ=255)
                                                                                                                                             aaaacCGGGAGTTCACATGAAGAGCAAAGGTAATAAGCAACAGACAGTAAATATGTTGAAAGCGATGGTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAc      <  1:250346/150‑1 (MQ=255)
                                                                                                                                                aaCTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTGGACAGAGATTGTCGCTATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcg  <  1:639157/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG  >  NC_000913/3206306‑3206600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑