Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,508,6620TA48.7% 38.4 / 22.3 40E384D (GAA→GAT) yhfWphosphopentomutase‑related metalloenzyme superfamily protein
Reads supporting (aligned to +/- strand):  ref base T (6/14);  new base A (19/0);  total (26/14)
Fisher's exact test for biased strand distribution p-value = 3.34e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATC  >  NC_000913/3508518‑3508809
                                                                                                                                                |                                                                                                                                                    
tgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGGGGGGAAAATcaa                                                                                                                                                  >  1:210771/1‑148 (MQ=255)
  tttttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTTGGGCGCGGGAAAAATtacaca                                                                                                                                               >  2:566764/1‑150 (MQ=255)
        aaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGGGCGGGAAAAATTacaaaccgga                                                                                                                                          >  2:275736/1‑145 (MQ=255)
               agagaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACAC‑GGGAGccccccc                                                                                                                                 >  2:222805/1‑148 (MQ=255)
                gagaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGGGGGAAAAATTACACACGGGAAGccccc                                                                                                                                   >  1:12368/1‑148 (MQ=255)
                 agaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACAC‑GGTAGCCCCCACATc                                                                                                                              <  1:173193/151‑1 (MQ=255)
                      aaTATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCCCGGGAAAATTTACACCC‑GCTAGACCCCCCATCaca                                                                                                                           >  2:256444/1‑147 (MQ=255)
                        tatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATCACACAC‑CGGAGCCCCCCCCACAGaaa                                                                                                                         >  2:136434/1‑149 (MQ=255)
                            cAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCgtgg                                                                                                                   <  2:701275/151‑1 (MQ=255)
                             aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCgtggt                                                                                                                  <  2:508360/151‑1 (MQ=255)
                             aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGCGGGAAAAATCACACAC‑GGGAACCCCCCCATCAGAAAACgcgg                                                                                                                   >  2:47969/1‑150 (MQ=255)
                               aTGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGCGGGGGGGAAAAAACAAAAAC‑GGGAGGCCCCCAATCCGAAAgagaggg                                                                                                                  >  2:637744/1‑143 (MQ=255)
                                   tCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGGGGGGAAAATTACAAAC‑GGCAGCCCCCACCTCAGAAAGCGGGGGgcg                                                                                                               >  1:624910/1‑148 (MQ=255)
                                       gggCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACAcc                                                                                                         <  2:323260/150‑1 (MQ=255)
                                            aGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACAC‑GGTAGCCCCCACATCAGAAAACGTGGTGCGCACAcc                                                                                                         >  2:621009/1‑145 (MQ=255)
                                                    aCCCGTCGGATCAAAACTCATAGGGGGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCTCACCGCGCTGCGTAGCGa                                                                                           <  1:136481/151‑1 (MQ=255)
                                                         tCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGCGCGCGGGAAAAATTACACAC‑CGTAAACCCCACACCAGAAAGAGTGGGGGGGACACCGAGCATCGTAg                                                                                              >  2:507462/1‑143 (MQ=255)
                                                                aaaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACAC‑GGGAGCCCCCCCCTCAGAAAACGGGGGGCGCACACCCAGCTGCGGAGCGATCATCCCtt                                                                                  >  2:587566/1‑148 (MQ=255)
                                                                       aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGAAAAAATTACACAC‑GGTAGCCCCCACATCAGAAAACGGGGGGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATaaa                                                                         >  2:76915/1‑150 (MQ=255)
                                                                       aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAGGGGGGCGGGAAAAGACACAAAC‑CGCAACCCCCACAATCGAGAGCGTGGGGCGCACACCCAACTCCGCAGCGATCATccc                                                                                    >  1:41074/1‑139 (MQ=255)
                                                                            gtgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCa                                                                   <  1:29763/151‑1 (MQ=255)
                                                                                      cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGGGGGAAAAATCAAACAA‑GGGAACCCCCACCTCAAAAAAGGGGGGGGCCACACCCAGCCGCGTAGCGACCACCCCCTCCTGGTAAAACAGCACCAGccccac                                                         >  1:354926/1‑151 (MQ=255)
                                                                                          ccGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACAC‑CGTAGCCCCCACATCAGAAAGCGGGGGGCGCACACCGAGCTGCGTAGCGAACAACCCCTTCTGGTAAACCAGCACTGGccccac                                                         >  2:178406/1‑147 (MQ=255)
                                                                                            ggagggagGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAATCACACAC‑GGTAGCCCCCCCATCAGAAAAGGGGGTGCGCACACCGAGCTGCGGAGCGATCATCCCTTGCTTATAAACCAGCACCGGCACCCCtt                                                       >  2:456501/1‑147 (MQ=255)
                                                                                             gagggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCgg                                                  <  2:210816/151‑1 (MQ=255)
                                                                                                ggaggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGGCCGGGAAAAATCACAAAC‑GGCAACCCCCCCAACCGAAAACGGGGGGCGCACACCCAGCCGCGGAACGAACATCCCCTTCCGATAAAACAGCACCCGCCCCACCTCCCggg                                                 >  1:58521/1‑149 (MQ=255)
                                                                                                         aaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCtg                                      <  2:410624/151‑1 (MQ=255)
                                                                                                           aagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCtgtg                                    <  2:558034/151‑1 (MQ=255)
                                                                                                                cGACCATTTTGTGGCGGTGGGGCGCGGAAAAAATAACACAC‑GGGAGCCCCCCCAACAGAAAGCGGGGGGCGCACACCCAGCAGCGGAGCGATCATCCCCTGCTGATAAAACAACACCGGCCCCACTTCGCGGGGATGGTGGCggtg                                    >  1:658634/1‑146 (MQ=255)
                                                                                                                    cATTTTGTGGCGGTGGCGCGCGGAAAAAATCACACAC‑GGTAGCCCCCCCATCAGAAAACGTGGGGCGCACACCGAGCTGCGTAGCGATCATCCCTTTCTGATAAACCAGCACCGGCACCACCTCGCGGGGATGGTGGGTGTGACCAAAgg                            >  2:669263/1‑150 (MQ=255)
                                                                                                                           tggcggtggcgCGCGGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATc                     >  1:483062/1‑150 (MQ=255)
                                                                                                                              cggtggcgCGCGGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTg                 <  1:256389/151‑1 (MQ=255)
                                                                                                                                gtggcgCGCGGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGcc               <  2:392991/151‑1 (MQ=255)
                                                                                                                                     gcgcgGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGAt          <  2:12369/151‑1 (MQ=255)
                                                                                                                                     gcgcgGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGAt          >  1:202009/1‑151 (MQ=255)
                                                                                                                                     gcgcgGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGAt          >  2:462251/1‑151 (MQ=255)
                                                                                                                                        cgGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCg       >  1:398211/1‑151 (MQ=255)
                                                                                                                                          gAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGcc     >  1:520802/1‑151 (MQ=255)
                                                                                                                                             aaaTTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCAAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATc  <  2:63216/151‑1 (MQ=255)
                                                                                                                                             aaaTTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTAGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATc  <  2:562015/151‑1 (MQ=255)
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TGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACAC‑GGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATC  >  NC_000913/3508518‑3508809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑