Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,724,971	A→C	F259C (TTC→TGC)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,724,971	0	A	C	100.0%	195.2 / NA	50	F259C (TTC→TGC)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/19);  total (31/19)

GCGACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCC  >  NC_000913/2724823‑2725117                                                                                                                                                     |                                                                                                                                                   gCGACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGcaa                                                                                                                                                  <  2:288485/151‑1 (MQ=255) gCGACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGcaa                                                                                                                                                  <  1:27201/151‑1 (MQ=255)         tCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGCTACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAggg                                                                                                                                           >  1:488110/1‑150 (MQ=255)          cTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCAACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAggg                                                                                                                                           >  2:308078/1‑149 (MQ=255)            tATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGcc                                                                                                                                       >  1:230407/1‑151 (MQ=255)            tATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGc                                                                                                                                        <  2:302235/150‑1 (MQ=255)                 gACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGc                                                                                                                                    >  1:410585/1‑149 (MQ=255)                       gcgcCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTa                                                                                                                             >  1:511132/1‑150 (MQ=255)                       gcgcCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTa                                                                                                                             >  2:695260/1‑150 (MQ=255)                           cAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAAcc                                                                                                                        <  1:529457/151‑1 (MQ=255)                              tGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCg                                                                                                                       >  2:149913/1‑149 (MQ=255)                                aGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGTGCCCGCAGCGGTAAAACCgag                                                                                                                     <  1:536815/149‑1 (MQ=255)                                      tGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCatg                                                                                                              <  1:37727/150‑1 (MQ=255)                                        aaTAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCatgatg                                                                                                           >  1:281862/1‑151 (MQ=255)                                               aTGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCAc                                                                                                    >  2:707535/1‑151 (MQ=255)                                               aTGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCACTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCAc                                                                                                    <  1:453753/151‑1 (MQ=255)                                                 gAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACgg                                                                                                  >  2:192320/1‑151 (MQ=255)                                                   aTACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCg                                                                                                <  2:534674/151‑1 (MQ=255)                                                      caaacaaTGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGAtt                                                                                             <  1:539693/151‑1 (MQ=255)                                                      caaacaaTGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGAtt                                                                                             >  2:260347/1‑151 (MQ=255)                                                                         aaTGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCaga                                                                          <  2:476969/151‑1 (MQ=255)                                                                          aTGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCag                                                                           >  2:711447/1‑149 (MQ=255)                                                                               aCTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCa                                                                    <  2:135967/151‑1 (MQ=255)                                                                               aCTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCa                                                                    <  1:389087/151‑1 (MQ=255)                                                                                   gCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAg                                                                   <  1:683063/148‑1 (MQ=255)                                                                                        gTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCtttt                                                           <  2:308539/151‑1 (MQ=255)                                                                                              atgcatTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAgcg                                                     >  2:79679/1‑151 (MQ=255)                                                                                              atgcatTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAgcg                                                     >  2:636512/1‑151 (MQ=255)                                                                                                  atTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGcgcgcg                                                 >  1:257273/1‑151 (MQ=255)                                                                                                   ttCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGt                                                >  2:547785/1‑151 (MQ=255)                                                                                                     cccGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGttt                                              >  1:529068/1‑151 (MQ=255)                                                                                                       cGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCt                                            <  2:25648/151‑1 (MQ=255)                                                                                                       cGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCt                                            <  1:555597/151‑1 (MQ=255)                                                                                                       cGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCt                                            >  2:259366/1‑151 (MQ=255)                                                                                                        gCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCtt                                           >  2:72208/1‑151 (MQ=255)                                                                                                         cAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCttg                                          <  2:540687/151‑1 (MQ=255)                                                                                                         cAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCttg                                          <  1:299542/151‑1 (MQ=255)                                                                                                             aTTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGc                                      >  2:436267/1‑151 (MQ=255)                                                                                                              tttACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCg                                     >  2:178946/1‑151 (MQ=255)                                                                                                                    aGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGttt                               >  2:161275/1‑151 (MQ=255)                                                                                                                    aGATACTTCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGttt                               >  2:605097/1‑151 (MQ=255)                                                                                                                          ttCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCt                          >  2:390551/1‑150 (MQ=255)                                                                                                                           tCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCt                          <  2:33146/149‑1 (MQ=255)                                                                                                                           tCTGCATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTa                         <  1:649168/150‑1 (MQ=255)                                                                                                                               cATATAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTg                     >  1:700044/1‑150 (MQ=255)                                                                                                                                atatAAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAATACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTgac                   >  2:685204/1‑151 (MQ=255)                                                                                                                                   taAGTAGTGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTgacga                 >  1:343269/1‑150 (MQ=255)                                                                                                                                       tagtGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTa             >  1:252111/1‑150 (MQ=255)                                                                                                                                         gtGAAGGTATAGCAACAAAGGGAGCCCGCAGCGGGAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCTAAGGTTCATCGTACTGACGACGTaa            <  1:612862/149‑1 (MQ=255)                                                                                                                                            aaGGTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACcac        >  2:354969/1‑150 (MQ=255)                                                                                                                                              ggTATAGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAAc     >  1:201335/1‑151 (MQ=255)                                                                                                                                                  taGCAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGcc  >  1:499752/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GCGACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCC  >  NC_000913/2724823‑2725117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑