Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,186,605 A→C H419P (CAC→CCC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,186,6050AC98.0% 172.2 / NA 49H419P (CAC→CCC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (28/20);  minor base T (1/0);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCT  >  NC_000913/4186465‑4186750
                                                                                                                                            |                                                                                                                                                 
ggCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTAct                                                                                                                                          <  2:229135/150‑1 (MQ=255)
        aGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCg                                                                                                                                 >  1:602667/1‑151 (MQ=255)
         gCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGt                                                                                                                                <  1:537183/151‑1 (MQ=255)
          cTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTg                                                                                                                               >  2:500014/1‑151 (MQ=255)
           tGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGc                                                                                                                              >  1:27584/1‑151 (MQ=255)
            gTTCAAACCGTCCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTg                                                                                                                               <  1:97566/149‑1 (MQ=255)
                 aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGCAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGAc                                                                                                                        >  1:625552/1‑151 (MQ=255)
                        cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCAc                                                                                                                 <  2:617676/151‑1 (MQ=255)
                               ggCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                                                                                          <  1:451652/151‑1 (MQ=255)
                                        gAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAgg                                                                                                 <  2:291848/151‑1 (MQ=255)
                                            tGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt                                                                                             >  2:175356/1‑151 (MQ=255)
                                             gCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAttt                                                                                            >  2:43257/1‑151 (MQ=255)
                                             gCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAttt                                                                                            >  1:224175/1‑151 (MQ=255)
                                              cGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTg                                                                                           <  1:125331/151‑1 (MQ=255)
                                                   tCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCg                                                                                      >  2:309/1‑151 (MQ=255)
                                                      tGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGt                                                                                    <  2:141259/150‑1 (MQ=255)
                                                          accaccATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTg                                                                                <  1:254534/150‑1 (MQ=255)
                                                             accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCg                                                                            >  1:297957/1‑151 (MQ=255)
                                                                         gCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCt                                                                 >  1:503563/1‑150 (MQ=255)
                                                                          cGAAGAAAATGTTTGAGCGCGAAGAAGCTGTCGTTTGGGATATTGTGGACGAAGTTATCCGCAAACTTCAGGAACTGCTGAATCGAGCACTTACTCTGCACAGATTCGGTATCCAGGCATTTGTACCGGCACTGATCGAAGGTAAAGCTAt                                                               >  1:577969/1‑151 (MQ=255)
                                                                             agaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCa                                                            <  1:633958/151‑1 (MQ=255)
                                                                              gaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATcc                                                             >  1:234639/1‑149 (MQ=255)
                                                                               aaaaTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGc                                                          >  1:206838/1‑151 (MQ=255)
                                                                               aaaaTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGc                                                          <  1:619823/151‑1 (MQ=255)
                                                                                     gTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGGACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGACGGTAAAGCTATCCAGCTGCAcc                                                    >  1:145208/1‑151 (MQ=255)
                                                                                        gAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCg                                                  <  2:675171/150‑1 (MQ=255)
                                                                                         aGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCt                                                >  2:650583/1‑151 (MQ=255)
                                                                                         aGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCt                                                <  2:62908/151‑1 (MQ=255)
                                                                                         aGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCt                                                >  2:209023/1‑151 (MQ=255)
                                                                                           cgcgAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTgg                                              >  2:481839/1‑151 (MQ=255)
                                                                                                agaagCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTtgt                                         >  1:661130/1‑151 (MQ=255)
                                                                                                  aagCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGc                                       >  1:327098/1‑151 (MQ=255)
                                                                                                  aagCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGc                                       >  2:355925/1‑151 (MQ=255)
                                                                                                   agCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCg                                      >  2:332945/1‑151 (MQ=255)
                                                                                                    gCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCgg                                     >  1:131707/1‑151 (MQ=255)
                                                                                                       gTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCat                                  >  2:33536/1‑151 (MQ=255)
                                                                                                       gTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGCGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCat                                  <  1:171423/151‑1 (MQ=255)
                                                                                                         cGTTTGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCata                                 <  2:117521/150‑1 (MQ=255)
                                                                                                           tttGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCGCCCTCTGGTTTGTGCGGCatat                                <  1:81664/149‑1 (MQ=255)
                                                                                                             tGGGATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACg                            >  1:596057/1‑151 (MQ=255)
                                                                                                              gggATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACg                            <  2:159585/150‑1 (MQ=255)
                                                                                                               ggATATCCTGGACGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCGATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGcc                          <  1:547093/151‑1 (MQ=255)
                                                                                                                          aCGAAGTTATCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGCATCCAGGCATTTGAACCGGTACTGATCGCAGGTAAAGCTATACAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGacg                >  1:234323/1‑148 (MQ=255)
                                                                                                                                  aTCCGCGAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGAcc          >  1:280302/1‑148 (MQ=255)
                                                                                                                                     cgcgAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATgg    <  1:200953/151‑1 (MQ=255)
                                                                                                                                      gcgAACCCCCGGTACTGCTGAACCGTGCCCCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGc   <  2:27587/151‑1 (MQ=255)
                                                                                                                                      gcgAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGc   >  2:49580/1‑151 (MQ=255)
                                                                                                                                      gcgAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAACTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGc   >  1:178919/1‑151 (MQ=255)
                                                                                                                                       cgAACCCCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGCTGGCt  >  1:187962/1‑151 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
GGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCT  >  NC_000913/4186465‑4186750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑