Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913354,2820TG39.0% 161.5 / 12.6 95V526G (GTT→GGT) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (15/43);  new base G (37/0);  total (52/43)
Fisher's exact test for biased strand distribution p-value = 1.46e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

CCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGC  >  NC_000913/354132‑354430
                                                                                                                                                        |                                                                                                                                                    
ccTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑ggt‑ggt                                                                                                                                                      <  1:529436/151‑1 (MQ=255)
    tGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGtt                                                                                                                                                     <  1:676536/148‑1 (MQ=255)
       cTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGG‑GGGTgggggg                                                                                                                                               >  1:28718/1‑149 (MQ=255)
         ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGaa                                                                                                                                             <  1:686172/151‑1 (MQ=255)
         ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTgggggg                                                                                                                                               >  2:20312/1‑147 (MQ=255)
          gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGaaa                                                                                                                                            <  1:132819/151‑1 (MQ=255)
          gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGG‑GGGTtggg                                                                                                                                                 >  2:326566/1‑146 (MQ=255)
           ggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAg                                                                                                                                           <  1:1303578/151‑1 (MQ=255)
             cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTTggggg                                                                                                                                                >  1:1342931/1‑144 (MQ=255)
             cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTggggggga                                                                                                                                             >  2:926729/1‑143 (MQ=255)
                 cggggcGCTGACGGGTATCACTTTATGCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGGATCGGCTTGGGACGCGTGAGAGTGAAGAGAGTAACTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATg                                                                                                                                        <  1:684141/143‑1 (MQ=255)
                   tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGATgcg                                                                                                                                      >  2:909864/1‑148 (MQ=255)
                     aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGaa                                                                                                                                 <  1:832679/151‑1 (MQ=255)
                         tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGGGAAAGATGCGCTGAAAggg                                                                                                                             >  2:74797/1‑151 (MQ=255)
                         tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGG‑GGGTGGGGGGAAAGATGGGCTGAAAggg                                                                                                                             >  2:446225/1‑151 (MQ=255)
                          gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGTATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAgg                                                                                                                              <  1:328346/149‑1 (MQ=255)
                          gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAggg                                                                                                                             <  1:413299/150‑1 (MQ=255)
                               ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGCGCATCGGCTGGGGACGCGCGACATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAggg                                                                                                                                         >  1:468526/1‑131 (MQ=255)
                                tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtg                                                                                                                       <  1:513549/150‑1 (MQ=255)
                                tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGGAggggg                                                                                                                      >  2:1270599/1‑151 (MQ=255)
                                 aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGGTGCCGAAGTGGC‑GGG‑GGGTGGGGGGGAAGAAGGGGTGGAAGGGCAgggg                                                                                                                       >  1:130169/1‑147 (MQ=255)
                                  tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGG‑GGGTGGGGTGAAAGAAGCGCCGTAAGGGCAggtggcg                                                                                                                    >  2:36091/1‑151 (MQ=255)
                                   cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGT‑GGTGGGGGGGAAAG‑TGGGCTGAAAggggcgggggcg                                                                                                                    >  1:1262118/1‑150 (MQ=255)
                                   cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcgg                                                                                                                   <  2:1177029/151‑1 (MQ=255)
                                   cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcg                                                                                                                    <  1:390187/150‑1 (MQ=255)
                                    aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGG‑GGTTGGGGGGGAAGATGCGCTGGAAGGGCAggtgggggg                                                                                                                  >  1:1323113/1‑150 (MQ=255)
                                     cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGGGCTGGAAAGGCCgggggggggg                                                                                                                 >  1:1210485/1‑149 (MQ=255)
                                     cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggt                                                                                                                  <  2:1038849/150‑1 (MQ=255)
                                      tttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGGGCTGAAAGGGCAgggggcggggg                                                                                                                >  2:954538/1‑151 (MQ=255)
                                       ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtg                                                                                                                 <  2:811032/149‑1 (MQ=255)
                                       ttATTCTCGGGCGCACTGACGAGGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtg                                                                                                                 <  1:54252/149‑1 (MQ=255)
                                        tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtggc                                                                                                               <  2:97556/150‑1 (MQ=255)
                                         aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGC‑GGGTGGGGTGGAAGAAGCGCCGGAAGGGCAGgtggcgggggcg                                                                                                              >  2:800095/1‑150 (MQ=255)
                                          ttCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGAGGGGCTGAGAGGGGCggtggcggggggggt                                                                                                            >  1:254248/1‑147 (MQ=255)
                                          ttCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGG‑GGGTGGGGGGGAAGATGCGCTGGAAGGGGAgggggcggggg                                                                                                                >  1:1363527/1‑147 (MQ=255)
                                           tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGGAggggggggggggggtt                                                                                                           >  2:1242751/1‑146 (MQ=255)
                                              cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGttt                                                                                                           <  1:933985/148‑1 (MQ=255)
                                              cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGt                                                                                                         <  2:435561/150‑1 (MQ=255)
                                              cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTc                                                                                                        <  1:32031/151‑1 (MQ=255)
                                                ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGTGGCGGGGGCGgtttt                                                                                                         >  2:852655/1‑146 (MQ=255)
                                                   gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTcc                                                                                                   <  2:630535/151‑1 (MQ=255)
                                                     aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGTGGAAGATGCGCTGGAAGGGCAGGAGGGGGGGGCGGTTGTCATTcc                                                                                                   >  1:1342569/1‑149 (MQ=255)
                                                     aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGG‑GGGTGGGGGGAAAGAGGCGCCGAAAGGGCAGGGGGTGGGGGCGGTTGTCCTTcc                                                                                                   >  1:1076251/1‑149 (MQ=255)
                                                      cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGTTTGTCATTcc                                                                                                   >  2:911018/1‑148 (MQ=255)
                                                       tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTCATTCCGaa                                                                                                >  2:1261285/1‑150 (MQ=255)
                                                                aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGATGGTCCGAAAGGGCAGGGGGGGGGGGGGGTTGTTAATTCGaaa                                                                                               >  1:1248658/1‑142 (MQ=255)
                                                                 aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACa                                                                                     <  1:544930/151‑1 (MQ=255)
                                                                     aCGTTGCCGGGCAGCGGCGGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCt                                                                                 <  1:973419/151‑1 (MQ=255)
                                                                       gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGAAGGGCTGAAAAGGCAGGGGGGGGGGGCGTTTTTCATTCCGAAAGGGAGGGAAAGGCttg                                                                               >  2:122120/1‑149 (MQ=255)
                                                                           ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                            <  1:582192/150‑1 (MQ=255)
                                                                            cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCCGAaaagaggggaaggctgg                                                                               >  1:1040843/1‑135 (MQ=255)
                                                                             gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTGGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATc                                                                         <  1:314064/151‑1 (MQ=255)
                                                                                 aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGa                                                                     <  2:436909/151‑1 (MQ=255)
                                                                                 aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAAATGCGCTGGAAGGGGAGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGGCTGGGAGAACGTgg                                                                     >  2:359425/1‑150 (MQ=255)
                                                                                   cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtg                                                                   <  1:988201/151‑1 (MQ=255)
                                                                                   cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtg                                                                   <  1:1192492/151‑1 (MQ=255)
                                                                                    ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCAATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGAAGGGCTGCAAAGGCAGGGGGGGGGGGGGGTTGGTATTCCGAAAGAGGGGGACAGGTTGGAAGAttggggt                                                                    >  1:1051922/1‑143 (MQ=255)
                                                                                      cTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGG‑GGGTGGGGGGGAAGATGCGCTGTAAGGGCAGGGGGGGGGGGCGGTTGTTATTCCGAAAGAGAGCGACAGGCTGGAAGATCGGGATTTgg                                                                >  2:341928/1‑151 (MQ=255)
                                                                                       tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTgg                                                                >  2:158766/1‑150 (MQ=255)
                                                                                       tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGG‑GGTTGGGGGGAAAGATGCGCCGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGGCAGTCTGGGAGATGGTGATGTggg                                                               >  1:79905/1‑150 (MQ=255)
                                                                                        ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTgg                                                                <  2:1379490/149‑1 (MQ=255)
                                                                                          ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTGgcg                                                              >  2:844272/1‑149 (MQ=255)
                                                                                              gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTTGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                         >  2:69143/1‑150 (MQ=255)
                                                                                              gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGTTAGAAGCGCTGGAAGGGGGGGGGGGGGGGGCGGTTGTTATTCCGAAAGAGAGGGACAGTGTGGGAGGTGGTGGTGGGGGGCACTCg                                                        >  1:175231/1‑151 (MQ=255)
                                                                                               cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGAAAGTCTGGAAGATCGGGATGTGGCGCACTCGc                                                       >  2:797770/1‑151 (MQ=255)
                                                                                                gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCa                                                      <  1:327443/151‑1 (MQ=255)
                                                                                                gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGGTTGTCCTTCCGAAAAAGAGCGACAGTCTGGGAGATCGTGGTGTGGCGCACt                                                          >  2:290868/1‑147 (MQ=255)
                                                                                                  gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGc                                                       >  2:543783/1‑148 (MQ=255)
                                                                                                       tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTTGGGTGGAAGATGCGCGGGAAGGGGCGGGGGCGGGGGCGGTTGTCATTTCGGAATAGAGACTCACAGTGGAAGATAGGGATGTGGCGCACCCGCAAgaga                                                 >  1:1119833/1‑149 (MQ=255)
                                                                                                         aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                             <  1:1276782/151‑1 (MQ=255)
                                                                                                             gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                         <  1:616501/151‑1 (MQ=255)
                                                                                                             gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGCGGGGGCGGTTGGCATTCCGAAAGAGAGCGAACGTCCTGAAGATCGTGATGTGGGGCACCCGCAAGAGaaag                                              >  1:11656/1‑144 (MQ=255)
                                                                                                                 aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATg                                      <  2:1028606/150‑1 (MQ=255)
                                                                                                                 aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGGGGAAGAAGCGCTGAAAGGGCAGGGGGCGGTGGCGGTTGTCATTCCGAAAGAGAGCAACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                     >  2:1207611/1‑151 (MQ=255)
                                                                                                                     ccAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgga                                    <  1:106977/148‑2 (MQ=255)
                                                                                                                     ccAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGCGGGGGGGGTTGTCATTCCGCAAGAGGGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGGGGTTATGgcg                                   >  2:1278163/1‑149 (MQ=255)
                                                                                                                      cAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGCGGGGGGGGTTTTCATTCCGAAAGAGAGGGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGGTTATGGCGCt                                 >  2:231432/1‑150 (MQ=255)
                                                                                                                       aGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtgg                               <  2:254305/151‑1 (MQ=255)
                                                                                                                         ccATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtg                             <  2:566164/151‑1 (MQ=255)
                                                                                                                           aTCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTAGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGTCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                            <  1:1153341/150‑1 (MQ=255)
                                                                                                                             ccGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa                         <  1:1149399/151‑1 (MQ=255)
                                                                                                                               gggCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGc                       <  2:28723/151‑1 (MQ=255)
                                                                                                                               gggCGTTGCCGAAGTGGC‑GGT‑GGGTCGGGGGGAAGATGCGCCGTAAGGGCAGGGGGGGGTGGGGTTTGTCCTTTCGCAAGAGAGGGACAGTCTGGAAGATCGGGGTGTGGCGGACTCGCCAGAGAAGGCGATTATGGGGCTGGGGGCCAg                        >  1:427564/1‑150 (MQ=255)
                                                                                                                               gggCGTTGCCGAAGTGGC‑GGC‑GGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGGGGGGGTTGTCCTTTCGGAAGAGAGCGAAAGTCTGGAAGATCGTGGGGTGGCGCACTCGCAAGAGAAGGGGGTTATGGGGCTGGGGGACa                         >  1:1143308/1‑149 (MQ=255)
                                                                                                                                ggCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGcc                      <  2:687141/151‑1 (MQ=255)
                                                                                                                                ggCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGcc                      <  1:558377/151‑1 (MQ=255)
                                                                                                                                 gCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCa                     <  1:121407/151‑1 (MQ=255)
                                                                                                                                 gCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGGGGAAGATGCGCTTGAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGGAAGAGAGCGGCAGGCTGGGAGAACGGGATGTGGCGCACTCGCAAGAGAAGGCGGATATGGCGCTGGGGGAGAGcc                      >  1:603795/1‑150 (MQ=255)
                                                                                                                                 gCGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGcc                      >  2:346779/1‑150 (MQ=255)
                                                                                                                                  cGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAg                    <  1:423497/151‑1 (MQ=255)
                                                                                                                                  cGTTGCCGAAGTGGC‑GGT‑GGGTGGGGGGAAAGAAGAGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTTATTCCGCAAAAGGGGGAAAGTCTGGGAGATCGCGGGGTGGGGCACCCCCAAGAGAAGGGGATTTTTGCGCTGGGGGACa                         >  1:1185402/1‑146 (MQ=255)
                                                                                                                                      gCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                 <  2:386582/150‑1 (MQ=255)
                                                                                                                                      gCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                 <  2:1041610/150‑1 (MQ=255)
                                                                                                                                             tGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGCGGACAGCCAGATTGGCAACtt         >  1:1085917/1‑151 (MQ=255)
                                                                                                                                             tGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGCGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt         >  2:85638/1‑151 (MQ=255)
                                                                                                                                                c‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTgg      >  2:1135526/1‑151 (MQ=255)
                                                                                                                                                   gt‑ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCCCTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc    >  1:349730/1‑151 (MQ=255)
                                                                                                                                                   gt‑ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc    >  2:135829/1‑151 (MQ=255)
                                                                                                                                                   gt‑ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc    <  1:40989/151‑1 (MQ=255)
                                                                                                                                                      ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgc  >  2:709727/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                    
CCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGC  >  NC_000913/354132‑354430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑