Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	39.0%	163.0 / 33.0	100	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (13/48);  new base G (39/0);  total (52/48)
Fisher's exact test for biased strand distribution p-value = 1.08e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCG  >  NC_000913/354141‑354436                                                                                                                                                     |                                                                                                                                                     ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGaa                                                                                                                                                   <  1:686172/151‑1 (MQ=255)  gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGaaa                                                                                                                                                  <  1:132819/151‑1 (MQ=255)   ggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAg                                                                                                                                                 <  1:1303578/151‑1 (MQ=255)         cggggcGCTGACGGGTATCACTTTATGCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGGATCGGCTTGGGACGCGTGAGAGTGAAGAGAGTAACTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATg                                                                                                                                              <  1:684141/143‑1 (MQ=255)           tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATgcg                                                                                                                                            >  2:909864/1‑148 (MQ=255)             aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGaa                                                                                                                                       <  1:832679/151‑1 (MQ=255)                 tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAggg                                                                                                                                   >  2:74797/1‑151 (MQ=255)                 tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGAAAGATGGGCTGAAAggg                                                                                                                                   >  2:446225/1‑151 (MQ=255)                  gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGTATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAgg                                                                                                                                    <  1:328346/149‑1 (MQ=255)                  gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAggg                                                                                                                                   <  1:413299/150‑1 (MQ=255)                       ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGCGCATCGGCTGGGGACGCGCGACATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAggg                                                                                                                                               >  1:468526/1‑131 (MQ=255)                        tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtg                                                                                                                             <  1:513549/150‑1 (MQ=255)                        tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAggggg                                                                                                                            >  2:1270599/1‑151 (MQ=255)                         aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGGTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGAAGGGGTGGAAGGGCAgggg                                                                                                                             >  1:130169/1‑147 (MQ=255)                          tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGTGAAAGAAGCGCCGTAAGGGCAggtggcg                                                                                                                          >  2:36091/1‑151 (MQ=255)                           cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGTGGTGGGGGGGAAAG‑TGGGCTGAAAggggcgggggcg                                                                                                                          >  1:1262118/1‑150 (MQ=255)                           cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcgg                                                                                                                         <  2:1177029/151‑1 (MQ=255)                           cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcg                                                                                                                          <  1:390187/150‑1 (MQ=255)                            aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGTTGGGGGGGAAGATGCGCTGGAAGGGCAggtgggggg                                                                                                                        >  1:1323113/1‑150 (MQ=255)                             cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGGGCTGGAAAGGCCgggggggggg                                                                                                                       >  1:1210485/1‑149 (MQ=255)                             cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggt                                                                                                                        <  2:1038849/150‑1 (MQ=255)                              tttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGGGCTGAAAGGGCAgggggcggggg                                                                                                                      >  2:954538/1‑151 (MQ=255)                               ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtg                                                                                                                       <  2:811032/149‑1 (MQ=255)                               ttATTCTCGGGCGCACTGACGAGGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtg                                                                                                                       <  1:54252/149‑1 (MQ=255)                                tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtggc                                                                                                                     <  2:97556/150‑1 (MQ=255)                                 aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGCGGGTGGGGTGGAAGAAGCGCCGGAAGGGCAGgtggcgggggcg                                                                                                                    >  2:800095/1‑150 (MQ=255)                                  ttCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGAGGGGCTGAGAGGGGCggtggcggggggggt                                                                                                                  >  1:254248/1‑147 (MQ=255)                                  ttCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGCTGGAAGGGGAgggggcggggg                                                                                                                      >  1:1363527/1‑147 (MQ=255)                                   tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAggggggggggggggtt                                                                                                                 >  2:1242751/1‑146 (MQ=255)                                      cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGttt                                                                                                                 <  1:933985/148‑1 (MQ=255)                                      cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGt                                                                                                               <  2:435561/150‑1 (MQ=255)                                      cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTc                                                                                                              <  1:32031/151‑1 (MQ=255)                                        ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGTGGCGGGGGCGgtttt                                                                                                               >  2:852655/1‑146 (MQ=255)                                           gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTcc                                                                                                         <  2:630535/151‑1 (MQ=255)                                             aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGTGGAAGATGCGCTGGAAGGGCAGGAGGGGGGGGCGGTTGTCATTcc                                                                                                         >  1:1342569/1‑149 (MQ=255)                                             aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGAAAGAGGCGCCGAAAGGGCAGGGGGTGGGGGCGGTTGTCCTTcc                                                                                                         >  1:1076251/1‑149 (MQ=255)                                              cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGTTTGTCATTcc                                                                                                         >  2:911018/1‑148 (MQ=255)                                               tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTCATTCCGaa                                                                                                      >  2:1261285/1‑150 (MQ=255)                                                        aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGGTCCGAAAGGGCAGGGGGGGGGGGGGGTTGTTAATTCGaaa                                                                                                     >  1:1248658/1‑142 (MQ=255)                                                         aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACa                                                                                           <  1:544930/151‑1 (MQ=255)                                                         aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACa                                                                                           <  2:989810/151‑1 (MQ=255)                                                             aCGTTGCCGGGCAGCGGCGGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCt                                                                                       <  1:973419/151‑1 (MQ=255)                                                               gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGGGCTGAAAAGGCAGGGGGGGGGGGCGTTTTTCATTCCGAAAGGGAGGGAAAGGCttg                                                                                     >  2:122120/1‑149 (MQ=255)                                                                   ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                  <  1:582192/150‑1 (MQ=255)                                                                    cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCCGAaaagaggggaaggctgg                                                                                     >  1:1040843/1‑135 (MQ=255)                                                                     gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTGGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATc                                                                               <  1:314064/151‑1 (MQ=255)                                                                         aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGa                                                                           <  2:436909/151‑1 (MQ=255)                                                                         aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAAATGCGCTGGAAGGGGAGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGGCTGGGAGAACGTgg                                                                           >  2:359425/1‑150 (MQ=255)                                                                           cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtg                                                                         <  1:988201/151‑1 (MQ=255)                                                                           cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtg                                                                         <  1:1192492/151‑1 (MQ=255)                                                                            ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCAATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGGGCTGCAAAGGCAGGGGGGGGGGGGGGTTGGTATTCCGAAAGAGGGGGACAGGTTGGAAGAttggggt                                                                          >  1:1051922/1‑143 (MQ=255)                                                                              cTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGCTGTAAGGGCAGGGGGGGGGGGCGGTTGTTATTCCGAAAGAGAGCGACAGGCTGGAAGATCGGGATTTgg                                                                      >  2:341928/1‑151 (MQ=255)                                                                               tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTgg                                                                      >  2:158766/1‑150 (MQ=255)                                                                               tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGTTGGGGGGAAAGATGCGCCGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGGCAGTCTGGGAGATGGTGATGTggg                                                                     >  1:79905/1‑150 (MQ=255)                                                                                ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTgg                                                                      <  2:1379490/149‑1 (MQ=255)                                                                                  ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTGgcg                                                                    >  2:844272/1‑149 (MQ=255)                                                                                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTTGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                               >  2:69143/1‑150 (MQ=255)                                                                                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGTTAGAAGCGCTGGAAGGGGGGGGGGGGGGGGCGGTTGTTATTCCGAAAGAGAGGGACAGTGTGGGAGGTGGTGGTGGGGGGCACTCg                                                              >  1:175231/1‑151 (MQ=255)                                                                                       cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGAAAGTCTGGAAGATCGGGATGTGGCGCACTCGc                                                             >  2:797770/1‑151 (MQ=255)                                                                                        gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCa                                                            <  1:327443/151‑1 (MQ=255)                                                                                        gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGGTTGTCCTTCCGAAAAAGAGCGACAGTCTGGGAGATCGTGGTGTGGCGCACt                                                                >  2:290868/1‑147 (MQ=255)                                                                                          gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGc                                                             >  2:543783/1‑148 (MQ=255)                                                                                               tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTTGGGTGGAAGATGCGCGGGAAGGGGCGGGGGCGGGGGCGGTTGTCATTTCGGAATAGAGACTCACAGTGGAAGATAGGGATGTGGCGCACCCGCAAgaga                                                       >  1:1119833/1‑149 (MQ=255)                                                                                                 aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                   <  1:1276782/151‑1 (MQ=255)                                                                                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                               <  1:616501/151‑1 (MQ=255)                                                                                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGCGGGGGCGGTTGGCATTCCGAAAGAGAGCGAACGTCCTGAAGATCGTGATGTGGGGCACCCGCAAGAGaaag                                                    >  1:11656/1‑144 (MQ=255)                                                                                                         aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATg                                            <  2:1028606/150‑1 (MQ=255)                                                                                                         aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGAAGCGCTGAAAGGGCAGGGGGCGGTGGCGGTTGTCATTCCGAAAGAGAGCAACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                           >  2:1207611/1‑151 (MQ=255)                                                                                                             ccAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgga                                          <  1:106977/148‑2 (MQ=255)                                                                                                             ccAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGCGGGGGGGGTTGTCATTCCGCAAGAGGGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGGGGTTATGgcg                                         >  2:1278163/1‑149 (MQ=255)                                                                                                              cAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGCGGGGGGGGTTTTCATTCCGAAAGAGAGGGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGGTTATGGCGCt                                       >  2:231432/1‑150 (MQ=255)                                                                                                               aGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtgg                                     <  2:254305/151‑1 (MQ=255)                                                                                                                 ccATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtg                                   <  2:566164/151‑1 (MQ=255)                                                                                                                   aTCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTAGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGTCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                                  <  1:1153341/150‑1 (MQ=255)                                                                                                                     ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa                               <  1:1149399/151‑1 (MQ=255)                                                                                                                       gggCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGc                             <  2:28723/151‑1 (MQ=255)                                                                                                                       gggCGTTGCCGAAGTGGC‑GGTGGGTCGGGGGGAAGATGCGCCGTAAGGGCAGGGGGGGGTGGGGTTTGTCCTTTCGCAAGAGAGGGACAGTCTGGAAGATCGGGGTGTGGCGGACTCGCCAGAGAAGGCGATTATGGGGCTGGGGGCCAg                              >  1:427564/1‑150 (MQ=255)                                                                                                                       gggCGTTGCCGAAGTGGC‑GGCGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGGGGGGGTTGTCCTTTCGGAAGAGAGCGAAAGTCTGGAAGATCGTGGGGTGGCGCACTCGCAAGAGAAGGGGGTTATGGGGCTGGGGGACa                               >  1:1143308/1‑149 (MQ=255)                                                                                                                        ggCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGcc                            <  2:687141/151‑1 (MQ=255)                                                                                                                        ggCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGcc                            <  1:558377/151‑1 (MQ=255)                                                                                                                         gCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCa                           <  1:121407/151‑1 (MQ=255)                                                                                                                         gCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGCGCTTGAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGGAAGAGAGCGGCAGGCTGGGAGAACGGGATGTGGCGCACTCGCAAGAGAAGGCGGATATGGCGCTGGGGGAGAGcc                            >  1:603795/1‑150 (MQ=255)                                                                                                                         gCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGcc                            >  2:346779/1‑150 (MQ=255)                                                                                                                          cGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAg                          <  1:423497/151‑1 (MQ=255)                                                                                                                          cGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGAAGAGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTTATTCCGCAAAAGGGGGAAAGTCTGGGAGATCGCGGGGTGGGGCACCCCCAAGAGAAGGGGATTTTTGCGCTGGGGGACa                               >  1:1185402/1‑146 (MQ=255)                                                                                                                              gCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                       <  2:386582/150‑1 (MQ=255)                                                                                                                              gCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                       <  2:1041610/150‑1 (MQ=255)                                                                                                                                     tGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGCGGACAGCCAGATTGGCAACtt               >  1:1085917/1‑151 (MQ=255)                                                                                                                                     tGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGCGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt               >  2:85638/1‑151 (MQ=255)                                                                                                                                        c‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTgg            >  2:1135526/1‑151 (MQ=255)                                                                                                                                           gtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCCCTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc          >  1:349730/1‑151 (MQ=255)                                                                                                                                           gtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc          >  2:135829/1‑151 (MQ=255)                                                                                                                                           gtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc          <  1:40989/151‑1 (MQ=255)                                                                                                                                             ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgc        >  2:709727/1‑151 (MQ=255)                                                                                                                                                tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg     >  2:1250156/1‑151 (MQ=255)                                                                                                                                                 ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg    >  1:263068/1‑151 (MQ=255)                                                                                                                                                 ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg    <  1:885558/151‑1 (MQ=255)                                                                                                                                                 ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg     <  2:1262972/150‑1 (MQ=255)                                                                                                                                                 ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg     <  1:74784/150‑1 (MQ=255)                                                                                                                                                   ggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAAGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcg  <  1:326462/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCG  >  NC_000913/354141‑354436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑