Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,181,852	A→C	K203T (AAA→ACA)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,181,852	0	A	C	100.0%	351.7 / NA	88	K203T (AAA→ACA)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (45/43);  total (45/43)

CGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCGTGGTGAAACCGC  >  NC_000913/4181706‑4181996                                                                                                                                                   |                                                                                                                                                 cGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACActg                                                                                                                                              <  2:671630/151‑1 (MQ=255) cGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACActg                                                                                                                                              >  1:1347851/1‑151 (MQ=255)  gTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACActgc                                                                                                                                             >  2:284592/1‑151 (MQ=255)  gTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACActgc                                                                                                                                             <  2:1269652/151‑1 (MQ=255)     ttcttTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACActgcctg                                                                                                                                          >  1:440665/1‑151 (MQ=255)      tcttTGACTCCGACATAGGTAAAACCCACTCTTCGGGTAAAGATCTGTATAACGCTCGTATCATCCCTTACCGTGGTTCCTGGCTGGACATCTAATTCGATCCGAAGGACAACCTGTTCTTACGTATCGACCGTCGCCATACActgc                                                                                                                                             <  1:1178573/147‑1 (MQ=255)      tcttTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACActgcct                                                                                                                                           <  1:1280523/149‑1 (MQ=255)         ttGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCg                                                                                                                                        <  2:1283609/149‑1 (MQ=255)           gACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGAcc                                                                                                                                     >  2:843021/1‑150 (MQ=255)            aCTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGAGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAATCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACcat                                                                                                                                   <  1:451655/151‑1 (MQ=255)                 gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTc                                                                                                                              >  2:171323/1‑151 (MQ=255)                 gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTc                                                                                                                              <  2:490531/151‑1 (MQ=255)                   cAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTg                                                                                                                            <  2:878946/151‑1 (MQ=255)                    aaaGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTCACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTgc                                                                                                                           >  1:1125012/1‑151 (MQ=255)                     aaGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTgcg                                                                                                                          <  2:1365518/151‑1 (MQ=255)                     aaGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTgcg                                                                                                                          >  1:225878/1‑151 (MQ=255)                      aGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTgcgc                                                                                                                         >  1:1205686/1‑151 (MQ=255)                      aGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTgcgc                                                                                                                         <  2:1124799/151‑1 (MQ=255)                          aaaaCCCAATCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGccc                                                                                                                     >  1:705538/1‑151 (MQ=255)                           aaaCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCt                                                                                                                    <  2:1301649/151‑1 (MQ=255)                             aCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTg                                                                                                                   >  2:301091/1‑150 (MQ=255)                              cccACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                                                  >  2:670839/1‑150 (MQ=255)                              cccACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                                                  >  1:802071/1‑150 (MQ=255)                              cccACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                                                  >  1:620560/1‑150 (MQ=255)                              cccACTCTTCGGGTAAAGTGCTGTATAACCCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCTCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTTaa                                                                                                                 <  1:1246461/151‑1 (MQ=255)                                       cGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTAcaccac                                                                                                        >  1:765648/1‑151 (MQ=255)                                        gggTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACcaca                                                                                                       <  2:572613/151‑1 (MQ=255)                                        gggTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCAACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTAcaccac                                                                                                        >  1:306384/1‑150 (MQ=255)                                         ggTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTAcaccac                                                                                                        >  2:325776/1‑149 (MQ=255)                                          gTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACaga                                                                                                     <  2:13847/151‑1 (MQ=255)                                                    gTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCt                                                                                            <  2:887415/150‑1 (MQ=255)                                                    gTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTc                                                                                           >  1:81296/1‑151 (MQ=255)                                                      ataACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGa                                                                                         <  2:355245/151‑1 (MQ=255)                                                       taACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGAc                                                                                        >  1:33589/1‑151 (MQ=255)                                                        aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGAc                                                                                        <  1:1173305/150‑1 (MQ=255)                                                         aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCt                                                                                      <  1:701209/151‑1 (MQ=255)                                                           gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGt                                                                                    >  1:874665/1‑151 (MQ=255)                                                            cgcgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGtt                                                                                   <  2:419043/151‑1 (MQ=255)                                                               gTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCtt                                                                                <  2:200269/151‑1 (MQ=255)                                                                 atcatcCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCttt                                                                               <  2:640599/150‑1 (MQ=255)                                                                 atcatcCCTTACCGTGGTTCCTGCCTGGACTTCGAATTCGATCCGAAGGACACCCTCTTCGTCCGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCttt                                                                               >  1:287468/1‑150 (MQ=255)                                                                 atcagcCCTTACCGTGGTTCCTGGCTGGACGTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTAACGACCGTCTCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCttt                                                                               <  1:184316/150‑1 (MQ=255)                                                                   catcCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGaa                                                                            <  1:75162/151‑1 (MQ=255)                                                                    atcCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGaaa                                                                           <  2:340713/151‑1 (MQ=255)                                                                      cccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGaaaa                                                                          <  2:766281/150‑1 (MQ=255)                                                                      cccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGaaaa                                                                          <  1:452620/150‑1 (MQ=255)                                                                        cTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGt                                                                       >  1:115893/1‑151 (MQ=255)                                                                        cTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGt                                                                       >  2:793245/1‑151 (MQ=255)                                                                         ttACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGtt                                                                      >  1:125921/1‑151 (MQ=255)                                                                            ccGTGATTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATc                                                                   >  1:1030450/1‑151 (MQ=255)                                                                             cGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCt                                                                  <  2:16673/151‑1 (MQ=255)                                                                                ggTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTg                                                               <  1:966481/151‑1 (MQ=255)                                                                                   tCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGaa                                                             >  2:1073276/1‑150 (MQ=255)                                                                                    ccTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGaaa                                                            <  2:828905/150‑1 (MQ=255)                                                                                     ctggctggACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGAACCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATc                                                          >  2:603294/1‑151 (MQ=255)                                                                                      tggctggACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATcc                                                         >  2:748155/1‑151 (MQ=255)                                                                                       ggctggACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCg                                                        <  1:564202/151‑1 (MQ=255)                                                                                       ggctggACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCg                                                        <  1:797897/151‑1 (MQ=255)                                                                                          tggACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTg                                                      >  1:1280700/1‑150 (MQ=255)                                                                                           ggACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGAt                                                    <  1:670610/151‑1 (MQ=255)                                                                                             aCTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATa                                                   >  1:1207213/1‑150 (MQ=255)                                                                                              cTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATaa                                                  <  1:76058/150‑1 (MQ=255)                                                                                                    aTTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                                           >  1:255929/1‑151 (MQ=255)                                                                                                    aTTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                                           >  1:110239/1‑151 (MQ=255)                                                                                                     ttCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGc                                          >  1:1350381/1‑151 (MQ=255)                                                                                                      tCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGc                                          >  1:1063035/1‑150 (MQ=255)                                                                                                       cGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGc                                          >  2:715866/1‑149 (MQ=255)                                                                                                          tCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATTATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTCTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATg                                     >  2:772172/1‑151 (MQ=255)                                                                                                           ccGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATgg                                    <  2:302373/151‑1 (MQ=255)                                                                                                            cGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATgg                                    >  2:92309/1‑150 (MQ=255)                                                                                                             gAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGa                                   >  2:791477/1‑150 (MQ=255)                                                                                                                 gACAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTgg                              >  1:1200467/1‑151 (MQ=255)                                                                                                                  aCAACCTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGAAACTGGt                             <  1:455838/151‑1 (MQ=255)                                                                                                                       cTGTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCgg                        >  1:459967/1‑151 (MQ=255)                                                                                                                         gTTCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGaa                      <  2:1206519/151‑1 (MQ=255)                                                                                                                           tCGTACGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAAc                     <  2:174294/150‑1 (MQ=255)                                                                                                                               acgtaTCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCt                 <  2:870567/150‑1 (MQ=255)                                                                                                                                 gtaTCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCg              <  1:538708/151‑1 (MQ=255)                                                                                                                                  taTCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCgt             >  1:822976/1‑151 (MQ=255)                                                                                                                                   aTCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCgtg            >  2:1147894/1‑151 (MQ=255)                                                                                                                                   aTCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCgt             <  2:1239353/150‑1 (MQ=255)                                                                                                                                     cGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCgtggt          <  1:174436/151‑1 (MQ=255)                                                                                                                                     cGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCgtgg           <  2:1096783/150‑1 (MQ=255)                                                                                                                                       aCCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCgtggtg         >  2:1291824/1‑150 (MQ=255)                                                                                                                                        ccGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCGTGGTGaa       <  2:438200/151‑1 (MQ=255)                                                                                                                                        ccGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCGTGGTGa        >  1:866332/1‑150 (MQ=255)                                                                                                                                         cGTCGCCGTACACTGCCTGCGACCATCATCCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGATGCTGGTACGCCTGCGCGGAGaa       >  2:562962/1‑150 (MQ=255)                                                                                                                                             gCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCGTGGTGAAACCGc  >  2:525682/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCGTGGTGAAACCGC  >  NC_000913/4181706‑4181996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑