Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,196,089	0	G	T	42.4%	85.5 / 30.0	66	T4K (ACA→AAA)	thiC	phosphomethylpyrimidine synthase
Reads supporting (aligned to +/- strand):  ref base G (12/26);  new base T (28/0);  total (40/26)
Fisher's exact test for biased strand distribution p-value = 2.15e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCG  >  NC_000913/4195941‑4196238                                                                                                                                                     |                                                                                                                                                        cGGGCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTttgt                                                                                                                                                        <  1:29830/150‑1 (MQ=255)  gggCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTttgttg                                                                                                                                                      <  2:724162/151‑1 (MQ=255)     cTAAGCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTttgtt                                                                                                                                                       >  2:491305/1‑147 (MQ=255)       aaGCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCCGGtttttt                                                                                                                                                       >  1:386065/1‑145 (MQ=255)       aaGCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGa                                                                                                                                                  <  2:241379/150‑1 (MQ=255)        aGCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTtttttt                                                                                                                                                       >  2:104109/1‑144 (MQ=255)         gCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACAt                                                                                                                                               <  2:340344/151‑1 (MQ=255)           tGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTTTTTCAg                                                                                                                                                   >  1:853637/1‑145 (MQ=255)           tGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTGTTGCAGACa                                                                                                                                                >  2:643880/1‑148 (MQ=255)            ggATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATa                                                                                                                                              <  2:1013085/149‑1 (MQ=255)              aTCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTGCAGACATAGCTc                                                                                                                                          >  2:669934/1‑151 (MQ=255)                    cGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGGAGACATAGCTCA‑tttc                                                                                                                                    >  2:97436/1‑148 (MQ=255)                    cGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTTGCGACATAGCCCA‑TTcc                                                                                                                                    >  1:541811/1‑150 (MQ=255)                       aTCGGCACACGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCAAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTTAGTTTTTTTGGATAAAAAACTCA‑TTTCa‑a                                                                                                                                 >  1:964641/1‑149 (MQ=255)                           gcacgcgcacgcCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGtt                                                                                                                           <  1:259974/151‑1 (MQ=255)                            cacgcgcacgcCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCCTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTa                                                                                                                          <  2:1032433/151‑1 (MQ=255)                              cgcgcacgcCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAg                                                                                                                        <  2:145029/151‑1 (MQ=255)                               gcgcacgcCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGGTTTTTTTCAGACAAAGTTCC‑TTTCA‑AAAAGTTAAgg                                                                                                                       >  1:935715/1‑151 (MQ=255)                                cgcacgcCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTGCCGGCATAGCTCA‑ATCCACAAAAGt                                                                                                                            >  1:711491/1‑146 (MQ=255)                                   acgcCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGGGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTGTTGTTTGTGACATAGCTCA‑TTCCC‑AAAAGTTagtgaggt                                                                                                                   >  2:736913/1‑144 (MQ=255)                                    cgcCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCCGGTTTTTTGTCGACATAGCTCA‑TTCCC‑AAAAAGTAAGGACggg                                                                                                                  >  1:82733/1‑149 (MQ=255)                                    cgcCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCCGGTTTTTTGTAGACATAGCTCC‑CTCCA‑AAAAGGTTAGGACGTg                                                                                                                  >  1:598086/1‑151 (MQ=255)                                       cGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCt                                                                                                               <  1:294421/151‑1 (MQ=255)                                         gggTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAACTCA‑TTCCA‑AAAAGTTAAGGAAGTGGGtt                                                                                                              >  2:892939/1‑150 (MQ=255)                                           gtgtgtGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGGTTTTTTGGAGACAAAGCTCA‑TTCCC‑AAAAGATAAGGAGGTGGGTTGt                                                                                                            >  2:357672/1‑150 (MQ=255)                                             gtgtgCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAg                                                                                                         <  1:453093/151‑1 (MQ=255)                                              tgtgCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTTCAGACATAGCTCC‑TTTCC‑AAAAGTTAAGGACGTGGGtt                                                                                                              >  2:129684/1‑145 (MQ=255)                                              tgtgCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGGTTTGTTGCAGGCAAAACTCA‑TTCCC‑AAAAATTTAGGGCGTggggtgt                                                                                                            >  2:412323/1‑142 (MQ=255)                                                   cAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGGTTTTTTTGAGACATAGCTCA‑TTTCC‑AAAAGTTAAGGGCGTGGCTTGTCAgac                                                                                                       >  1:401352/1‑147 (MQ=255)                                                    aGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGGTTTTTTTCAGGAAAAGCCCC‑TTCCC‑AAAAGGTAAGGACGGGGGTTTTCAGGCGACgg                                                                                                  >  1:314563/1‑151 (MQ=255)                                                        atatAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGCTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTGCAGACATAGCTCA‑TTCCA‑AAAAAGTAAGGACGTGGGTTGTCAGACGACgg                                                                                                  >  2:320236/1‑147 (MQ=255)                                                          ataAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGaa                                                                                             <  1:960149/150‑1 (MQ=255)                                                            aaaTGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTGGAGACATTGCTCC‑TTTCC‑AAAAGTTAAGGACGGGGGGTGTTAGAAGACgggggaa                                                                                             >  1:574997/1‑143 (MQ=255)                                                            aaaTGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTGCAGACATAGCTCA‑TTCCA‑AAAAGGTAAGGACGTGGCTTGTTAGACGACGGATGaa                                                                                             >  1:199663/1‑148 (MQ=255)                                                            aaaTGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGGTTTTTTTCAGACATAGACCA‑TTTCA‑AAAAAGTAAGGACGTGGGTTGTCAGGAGACGGGTGaa                                                                                             >  2:538785/1‑148 (MQ=255)                                                                gCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGGTTTTTTGCCGACAAAGCTCA‑TTTCA‑AAAAGATAAGGGCGGGGGGTTTCAGACGGCGGATGGAGCaa                                                                                         >  1:209790/1‑148 (MQ=255)                                                                   ttttGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGGTTTGTTTCAGACATAGCCCA‑TTCCC‑AAAAGGTAAAGACGGGGGGTTTCAGAAGACGGGTGaaagaa                                                                                         >  2:794609/1‑141 (MQ=255)                                                                   ttttGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGGTTTTTTTGAGACATAACTCATTTCCA‑AAAAATTAA‑GAAGTGGGTTTTCAGACGAAGGGTGaaagaa                                                                                         >  2:384469/1‑141 (MQ=255)                                                                    tttGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTTTTTCAGACAAAACTCA‑TTTCA‑AAAAAGTAAGGACGTGGGTTGTCAGAAGACGGGTGAAACAaga                                                                                       >  2:341934/1‑146 (MQ=255)                                                                       gAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATc                                                                                <  2:911853/150‑1 (MQ=255)                                                                       gAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGGTTTGTTGTAGACATAACTCA‑TTCCA‑AAAAGTTAAGGACGGGGGTTGTTAGACGACGGAAGGAGCAAAagaaga                                                                                  >  1:419786/1‑148 (MQ=255)                                                                        aGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTGCAGACATAGCTCC‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATcg                                                                               >  1:311705/1‑150 (MQ=255)                                                                         gTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTTCAGACATAGCTCA‑TTCCA‑AAAAATTAAGGACGTGGGTTGTCAGACGACGGGAGAAGCAAGAGAAGAAcgcc                                                                             >  2:492242/1‑151 (MQ=255)                                                                            gggAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTGTTTGAGACATAGCTCC‑TTTCA‑AAAAGGTAAGGACGGGGCTTGTCAGACGACGGGTGAAGCAAGAGACGATcgcc                                                                             >  1:464321/1‑148 (MQ=255)                                                                               aaaGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGGTTTTTTGCAGACAAAGCCCC‑TTTCA‑AAAAAATAAAGACGGGGGTTTTCAAACGACGGGTGAAAGAAAAGAAGAACGCCCCCggg                                                                       >  2:461821/1‑151 (MQ=255)                                                                                aaGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAgggg                                                                      <  2:895064/151‑1 (MQ=255)                                                                                 aGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGTAGATTTTTTGGAGACACAACTCC‑TTCCC‑AAAAGATAAGGACGGGGGTTGTCCGAAGAGGGCTGGAGAAAGAGACGAtatgc                                                                             >  1:847327/1‑139 (MQ=255)                                                                                       tGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCCGTTTTTTTTCAGACATAGCCCC‑CTTCC‑AAAAGTTAAGGGCGGGGGTTGTTCGACGACGGGTGAAGGAAAAGACGAACGCCCCCGGGgggaggcg                                                               >  1:576717/1‑151 (MQ=255)                                                                                        gCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgatgcg                                                               <  2:602750/150‑1 (MQ=255)                                                                                            tCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTTTTTCAGACATAGCTCA‑TTCCC‑AAAAAATAAGGACGCGGCTTGTCAGACGACGGATGAAACAAGAGACGAACGCCGCAGGGGGGATGCGATAg                                                           >  2:671297/1‑150 (MQ=255)                                                                                             ccAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGc                                                          <  1:636378/150‑1 (MQ=255)                                                                                              cAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAg                                                        <  2:532440/151‑1 (MQ=255)                                                                                                  gtgtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTa                                                    <  2:113824/151‑1 (MQ=255)                                                                                                    gtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTaa                                                   <  2:720785/150‑1 (MQ=255)                                                                                                    gtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTGCAGACATAGCTCA‑TTCCC‑AAAAGATAAGGACGTGGCTTGTTAGACGACGGATGAAGCAAGAGACGATAGCCCCAGGGGCGGTGCGGTAGCAGAtt                                                     >  1:451012/1‑148 (MQ=255)                                                                                                        aTAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGGTTTTTTGCAGACATAACTCA‑TTCCC‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGGTGAAGCAAGAGACGAACGCCGCAGGGGGGGTGCGATAGCAGAATTAATCtt                                              >  2:822321/1‑151 (MQ=255)                                                                                                          aaaaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTg                                             <  2:512762/150‑1 (MQ=255)                                                                                                          aaaaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTg                                             <  2:762144/150‑1 (MQ=255)                                                                                                            aaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTc                                          <  2:401402/151‑1 (MQ=255)                                                                                                             aTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTcc                                         <  1:323062/151‑1 (MQ=255)                                                                                                                   ggCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGTAGACATAGCTCA‑TTCCC‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCCGGGGGGGTGCGGTAGCAGATTAACTCTTGTTTCCCTc                                     >  1:735087/1‑149 (MQ=255)                                                                                                                        gggCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTa                              <  2:5128/151‑1 (MQ=255)                                                                                                                              gttgttCGCGGCGGGTCAGTTTTTTTGCAGAAAAAGCCCC‑CTCCA‑AAAAGATGAAGACGTGGGTTTTCTTAAGCCCGGTGAAAGAAAAAAAGAGCACCGCAGGGGGGGTGCGGTAACAGATTTACTCTTGTTTCCCTCggcgggtgtagcc                        >  1:198286/1‑139 (MQ=255)                                                                                                                              gttgttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGcc                        <  2:314607/151‑1 (MQ=255)                                                                                                                                 gttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGa                     <  2:357120/151‑1 (MQ=255)                                                                                                                                        gcggGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGtt              <  1:99956/151‑1 (MQ=255)                                                                                                                                        gcggGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGtt              >  1:571305/1‑151 (MQ=255)                                                                                                                                          gggTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTcc            <  1:699660/151‑1 (MQ=255)                                                                                                                                           ggTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCcg           >  1:468375/1‑151 (MQ=255)                                                                                                                                              cAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCgg        >  2:722176/1‑151 (MQ=255)                                                                                                                                                  tttGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATc     <  1:328501/150‑1 (MQ=255)                                                                                                                                                    tgttgCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCg  >  1:317805/1‑151 (MQ=255)                                                                                                                                                     gttgCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCg  <  1:568057/150‑1 (MQ=255)                                                                                                                                                     gttgCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCg  <  1:423544/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                        CGGGCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCA‑TTCCA‑AAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCG  >  NC_000913/4195941‑4196238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑