Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,451,123	C→A	V22V (GTC→GTA)	ytfR →	putative sugar ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,451,123	0	C	A	100.0%	302.5 / NA	76	V22V (GTC→GTA)	ytfR	putative sugar ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/44);  total (32/44)

GGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAG  >  NC_000913/4450975‑4451270                                                                                                                                                     |                                                                                                                                                    ggCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTaaa                                                                                                                                                   <  2:916937/151‑1 (MQ=255)     aTGGTGCTCTGAAGCCTGATGCGACGCTTACCTCATCTTATCATTCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCTACCAACACCAGGATATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCTGCGTAAAAGCg                                                                                                                                               <  1:367803/151‑1 (MQ=255)        gTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTa                                                                                                                                            <  1:639511/151‑1 (MQ=255)        gTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTa                                                                                                                                            <  2:464668/151‑1 (MQ=255)          gCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAg                                                                                                                                           <  2:91910/150‑1 (MQ=255)           cACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGAc                                                                                                                                         >  2:992442/1‑151 (MQ=255)               gAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACaa                                                                                                                                       <  2:1019444/149‑1 (MQ=255)               gAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACaa                                                                                                                                       <  1:709088/149‑1 (MQ=255)                 aGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGtt                                                                                                                                   <  2:37987/151‑1 (MQ=255)                      gATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGAttt                                                                                                                              <  2:164100/151‑1 (MQ=255)                       aTGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTc                                                                                                                             >  2:384485/1‑151 (MQ=255)                         gCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAg                                                                                                                           <  2:976856/151‑1 (MQ=255)                            aCGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCt                                                                                                                        <  2:414148/151‑1 (MQ=255)                             cGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTg                                                                                                                       >  1:337382/1‑151 (MQ=255)                               cTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTgcg                                                                                                                     >  2:711783/1‑151 (MQ=255)                               cTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTgcg                                                                                                                     >  1:305081/1‑151 (MQ=255)                                 tACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGcc                                                                                                                   >  2:400556/1‑151 (MQ=255)                                 tACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGcc                                                                                                                   >  2:315390/1‑151 (MQ=255)                                       gTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCg                                                                                                             <  2:301413/151‑1 (MQ=255)                                        tCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCg                                                                                                             >  1:665815/1‑150 (MQ=255)                                         cTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGa                                                                                                            >  1:771825/1‑150 (MQ=255)                                           tATCATTCCTACTGGGAGCACGCTTTACCCCGGGGGAAACCATGACGGCCGGCCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGc                                                                                                              <  2:603819/146‑1 (MQ=255)                                           tATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGa                                                                                                            <  1:710643/148‑1 (MQ=255)                                           tATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAAt                                                                                                         >  2:252476/1‑151 (MQ=255)                                                   cTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGaa                                                                                                           >  2:890243/1‑141 (MQ=255)                                                       tGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCgctgct                                                                                             >  1:761461/1‑151 (MQ=255)                                                        gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTc                                                                                            <  2:628410/151‑1 (MQ=255)                                                          gAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCgg                                                                                          <  1:580296/151‑1 (MQ=255)                                                               cGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGaa                                                                                      >  2:331870/1‑150 (MQ=255)                                                                 cTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAAc                                                                                   >  2:687229/1‑151 (MQ=255)                                                                  tttACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGCCAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACg                                                                                  >  1:247793/1‑151 (MQ=255)                                                                  tttACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAAc                                                                                   >  1:997762/1‑150 (MQ=255)                                                                   ttACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACgg                                                                                 >  2:445171/1‑151 (MQ=255)                                                                     acacCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACgggg                                                                               <  2:658795/151‑1 (MQ=255)                                                                        ccGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCGCCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCgg                                                                            <  1:640479/151‑1 (MQ=255)                                                                          gggggAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGa                                                                          <  2:269033/151‑1 (MQ=255)                                                                          gggggAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGa                                                                          <  2:833167/151‑1 (MQ=255)                                                                            gggAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGaaa                                                                        <  2:901972/151‑1 (MQ=255)                                                                              gAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAAt                                                                      <  2:452527/151‑1 (MQ=255)                                                                              gAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAAt                                                                      <  2:545654/151‑1 (MQ=255)                                                                              gAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAAt                                                                      <  1:703747/151‑1 (MQ=255)                                                                               aaaCCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATc                                                                     <  2:72558/151‑1 (MQ=255)                                                                                aaCCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATc                                                                     >  1:415804/1‑150 (MQ=255)                                                                                aaCCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCa                                                                    <  2:387570/151‑1 (MQ=255)                                                                                aaCCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAAGTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATc                                                                     <  1:257060/150‑1 (MQ=255)                                                                                    aTGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGc                                                                <  1:529053/151‑1 (MQ=255)                                                                                      gacgacCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCt                                                               <  1:159092/150‑1 (MQ=255)                                                                                        cgaccgacCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAAt                                                            <  2:215698/151‑1 (MQ=255)                                                                                            cgaccAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCaaa                                                        <  1:953566/151‑1 (MQ=255)                                                                                            cgaccAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCaa                                                         >  2:294908/1‑150 (MQ=255)                                                                                             gaccAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAg                                                       <  1:817791/151‑1 (MQ=255)                                                                                              accAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGc                                                      >  2:755790/1‑151 (MQ=255)                                                                                                cAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCAt                                                    <  2:100426/151‑1 (MQ=255)                                                                                                  acacCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCAtt                                                   >  1:120640/1‑150 (MQ=255)                                                                                                    acCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTaa                                                 >  2:776206/1‑150 (MQ=255)                                                                                                    acCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTaa                                                 >  2:574691/1‑150 (MQ=255)                                                                                                       aGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTgg                                             >  1:39747/1‑151 (MQ=255)                                                                                                            aTCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAATGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACTGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGtat                                        <  1:249030/151‑1 (MQ=255)                                                                                                              cctccGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGtata                                       <  2:985130/150‑1 (MQ=255)                                                                                                              cctccGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATAc                                      <  1:509055/151‑1 (MQ=255)                                                                                                                 ccGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCa                                    <  1:1031418/150‑1 (MQ=255)                                                                                                                 ccGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCAc                                   <  2:567844/151‑1 (MQ=255)                                                                                                                  cGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACg                                  <  2:253138/151‑1 (MQ=255)                                                                                                                     aCCGAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCg                               >  2:474917/1‑151 (MQ=255)                                                                                                                         aaGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATcg                           >  1:953846/1‑151 (MQ=255)                                                                                                                          aGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATcgc                          >  2:812721/1‑151 (MQ=255)                                                                                                                           ggATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGAAcgc                          >  1:50531/1‑150 (MQ=255)                                                                                                                            gATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCgg                        <  1:503065/151‑1 (MQ=255)                                                                                                                               tAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCa                      <  2:14132/150‑1 (MQ=255)                                                                                                                                 aGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCa                   <  1:1039569/151‑1 (MQ=255)                                                                                                                                  gTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCAt                  <  1:514180/151‑1 (MQ=255)                                                                                                                                        ttttCCCCGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATctggc             <  1:7785/150‑1 (MQ=255)                                                                                                                                            ccccGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGa         >  2:210259/1‑150 (MQ=255)                                                                                                                                             cccGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTAAACCACGCCGATCCAGGCACCATCTGGCTGGa         >  1:977099/1‑149 (MQ=255)                                                                                                                                              ccGGCGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAgg      >  1:585836/1‑151 (MQ=255)                                                                                                                                                  cGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGcc    >  2:1037363/1‑149 (MQ=255)                                                                                                                                                  cGTAAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCAGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAg  <  2:595359/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAG  >  NC_000913/4450975‑4451270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑