Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,222,596	0	G	A	42.6%	64.1 / 37.9	61	F13F (TTC→TTT)	ymgD	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (26/9);  new base A (0/26);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 7.21e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCACC  >  NC_000913/1222448‑1222745                                                                                                                                                        |                                                                                                                                                      cAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGaa                                                                                                                                                     <  1:979827/151‑1 (MQ=255)  aaCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGaaa                                                                                                                                                    <  1:251385/151‑1 (MQ=255)    cGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAaaac                                                                                                                                                  <  2:56550/151‑1 (MQ=255)       tGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAaaacaa                                                                                                                                                >  2:461398/1‑150 (MQ=255)       tGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAaaacaa                                                                                                                                                >  1:572608/1‑150 (MQ=255)          cattacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAaacaaaca                                                                                                                                             <  1:595914/150‑1 (MQ=255)           attacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAgg                                                                                                                                           <  2:234013/151‑1 (MQ=255)              acccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGccc                                                                                                                                        >  2:193113/1‑151 (MQ=255)                ccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGc                                                                                                                                      <  2:653866/151‑1 (MQ=255)                    taccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGt                                                                                                                                  >  1:647702/1‑151 (MQ=255)                      ccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTa                                                                                                                                 >  1:850093/1‑150 (MQ=255)                        gCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTaa                                                                                                                                >  2:675656/1‑149 (MQ=255)                          aacatTTTCTGCACCCTGTGCGCTGTCAGTTTTTGCC‑TTTTCTTTGGCTTCTGCGAAAGTAATATAGGTTTCTGACGGCAAA‑CCTTTTTTAATATTGTAGTCCTGCCCCCAGGTCATTGGTG‑CAAAAACAAACAGGCCCGCCAGTAAAGc                                                                                                                             <  2:297565/150‑1 (MQ=255)                          aacaaTTCCAGCAGCCTGTGCGCTGTCAGTTTTTGCC‑ATTTCTTTGCCTTCTGCGAAAAAAAAATAGGTTTCTAACGCCAAC‑CCTTTTTAAATATTTTAGTCCTGCGCCCAGGTAATGGTTG‑CAAAAAAAAACAGGCCCGCCAGTAAAg                                                                                                                              <  1:283363/149‑1 (MQ=255)                             aaTGTCTGCACCTTGTGCGCTGTCAGTTTTGGCC‑ATTTCGTGGGCTTCTGCGCAAGTAATATAGTTTTCTGACGGCAAC‑CCTTTTTTAATATTGTAGTCCTCCGCCAAGGTCATGGGGG‑AAAAAAAAAACAGCCCCGCCAGTAAAGCAAAt                                                                                                                         <  1:728712/151‑1 (MQ=255)                              aTTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAAtt                                                                                                                        >  2:838029/1‑151 (MQ=255)                                      acccTGTGCTCTGTCA‑TTTTTGCCTTTTTCGTTGGTTTCTGCGCAAGAAATATAGGTTGCTGCCGGCAAC‑CCTTTTTTAATATTGTAGTCCGGCGCCAAGTTTTTGGGTG‑AAAAAACAAACGGGCCCGCCAGTAAAGCAAAttttt                                                                                                                     <  1:205908/144‑1 (MQ=255)                                      aaCCTGTGCGCTGTCAGTTTTTGCC‑TTTTCGTTGGTTTCGGCAAAAATAATATAGTTTTCGGACGGCAAT‑CCTTTTTTAATTTTGTAGTCCTGCGCCCAGGTAATTGGTG‑GGAAAACAAAAAGGCCCGCCAGTAAAGCAAATTTTTtca                                                                                                                  <  2:953084/149‑1 (MQ=255)                                                 tGTCAGTTTTTGCT‑TTTTCGTTGGCTTCTGCGCAATTAATATGGTTTTCTGCCGGAAAA‑CCTTTTTTAATATTGTAGTCCTGCCCCCAGGTCATTGGTG‑CAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCt                                                                                                       <  2:953774/149‑1 (MQ=255)                                                  gTCATTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAC‑CCTTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCt                                                                                                       <  2:908861/148‑1 (MQ=255)                                                   tCATTTTTTGCC‑TTTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGAAAC‑CCTTTTTTAATATTGTAGCCCTGCGCCCAGGTCATTGGTG‑CAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTa                                                                                                     <  2:645445/149‑1 (MQ=255)                                                       tttttGCC‑TTTTCGTTGGTTTCTGCGCAAGTAAAATGGTTTTCTGAGGAAAAC‑CCTTTTTTAATATTGTAGCCCGGCGCCCGGGTCTTTGGTG‑CAAAAACAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTc                                                                                                         <  2:831234/141‑1 (MQ=255)                                                       tttttGCC‑ATTTCTTTGTCTTCTGCGCAAGAAATATAGGTTTCTGACGCAAAC‑CCTTTTTTAATATTGTAGCCCTGCCCCCAGTTCATTGGTG‑CAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCa                                                                                                <  1:461169/150‑1 (MQ=255)                                                        ttttGCC‑ATTTCTTTGGTTTCTGCGCAATTAATATAGTTTTCTGACGGAAAA‑CCTTTTTTAATTTTGTAGTCCGGCGCCCAGTTCTTGGGTG‑CAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAt                                                                                               <  1:639617/150‑1 (MQ=255)                                                             cc‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGTTTTCGGACGAAAAC‑CCGTTTTAAATATTGTAGTCCGGCGCCCAGTTCATTGGTG‑CAAAAAAAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAc                                                                                          <  1:819972/150‑1 (MQ=255)                                                             cc‑ATTGCGTTGGCTTCTGCGCAAGTAATAGGGTTTTCTGACGCCAAC‑CGTTTTTTAATTTTGTGGCCTTGCGCCCAGGTCATTGGTG‑CAAAAACAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAc                                                                                          <  2:860483/150‑1 (MQ=255)                                                              ccttttCTTTGGTTTCTGCGAAAGTAATATGGGTTTCTGACGAAAAC‑CCTTTTTAAATATTGTAGTCCTGCCCCCAGGTCATGGGTG‑AAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAc                                                                                          <  2:725216/147‑1 (MQ=255)                                                                aTTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAcc                                                                                         >  1:659968/1‑149 (MQ=255)                                                                 tttCGTTGTTTTCTGCGAAATAAATATAGGTTTCTGACGGAAAC‑CCTTTTTAAATTTTGTACTCCAGCCCCAATTTTTTTGTTT‑CAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAtttt                                                                                            <  1:180805/145‑1 (MQ=255)                                                                  tttttgggtttCTGCGAAAATAATATAGGTTTCTGCCGCCAAC‑CCTTTTTTAATATTGTATTCTTCCCCCCAGTTTTTTGTGG‑CAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAccc                                                                                        <  1:35801/139‑1 (MQ=255)                                                                   tctttGGCTTCTCCGAAAGTATTATAGGTTTCAAACGACAAA‑CCTTTTTTAATTTGGAAGCCCTGCTCCAAGTTTATTGGTG‑CAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGa                                                                                     <  1:640701/147‑1 (MQ=255)                                                                      ttggttttggctcAAATAATATAGTTTTTTGA‑GGCAAACCCTTTTTTAATTTTGTATTCCCCCGCCCAGTTTATGGTTG‑AGAAACCAAAAAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGaa                                                                                    <  2:789731/136‑1 (MQ=255)                                                                                cgcAATTAATATAGGTTTCTGCCGGCAAC‑CCGTTTTAAATTTTGTACCCCGGCGCCAAGATCTTTGGTG‑AGAAAACAAACGGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGcaccac                                                                           <  2:932552/147‑1 (MQ=255)                                                                                cgcAAGTAATATAGTTTTCTGACGCAAAC‑CCTTTTTTAATATTGTAGTCCTCCGCCCAGTTCATTGGTG‑CAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCaccacc                                                                          <  2:867972/148‑1 (MQ=255)                                                                                  cAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTAc                                                                     >  2:219718/1‑151 (MQ=255)                                                                                   aaGTAATATAGGTTTCTGACGGCAAC‑CCTTTTTTAATATTGTAGCCCTGCCCCCAGGTCTTTGGTG‑AAAAAAAAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTa                                                                      <  2:543196/149‑1 (MQ=255)                                                                                        atagAGTTTCCTGACGGAAAA‑CCTTTTTTAATATTGTAGCCCCGCGCCCAGGTAATTGGTG‑CAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCa                                                                <  1:456821/150‑1 (MQ=255)                                                                                          ataGTTTTCGGACGGCAAC‑CCTTTTTTAATATTGTATTCCTGCGCCCAGTTTTTGGGTG‑CAAAAAAAACCAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCa                                                                <  2:888762/148‑1 (MQ=255)                                                                                            cggatgctaaCGGCAAA‑CTGTTTGTACTATTGTAGTCCGGCGACCAGGCCATTGGTG‑CGAAAACAAACAGCCCCGCCAGAAAAGCAAATTTTTTCATCATCATTCCTTTTTTCATTTTACGCAGAGTTGCACCACCCGTAACGCAAGTCa                                                            >  1:480824/10‑150 (MQ=255)                                                                                             gttttgtgaccaaaaa‑CAGTTTTGAAT‑TTCTAGCCCCGCCCCAAAGTTATTTGTGACAAAAACAACCAGTCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCCCCACCCGTACCACCAATCTCg                                                          <  1:39369/138‑1 (MQ=255)                                                                                             ggTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACg                                                          >  2:205584/1‑151 (MQ=255)                                                                                              gTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACgg                                                         >  2:163307/1‑151 (MQ=255)                                                                                                 tCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCAc                                                      >  2:632639/1‑151 (MQ=255)                                                                                                     aCGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCttt                                                  >  2:249533/1‑151 (MQ=255)                                                                                                        gCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAAt                                               >  2:478202/1‑151 (MQ=255)                                                                                                         cAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATc                                              >  1:450945/1‑151 (MQ=255)                                                                                                              ccGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGccc                                          >  2:935326/1‑151 (MQ=255)                                                                                                                gTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCt                                        >  2:629936/1‑151 (MQ=255)                                                                                                                gTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCt                                        >  2:530497/1‑151 (MQ=255)                                                                                                                   tttAATTTTTTAGTCCCGCGCCCAGTTCATTGTTG‑AAAAAACAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTc                                      <  1:689920/150‑1 (MQ=255)                                                                                                                          ttGTACTCCCGCCCCCTGTTCATTGGTG‑CAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTA‑TTCATTTTACCCAGAATTGCACCCCCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCa                               <  1:357811/149‑1 (MQ=255)                                                                                                                           tGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCAtt                             >  2:345094/1‑151 (MQ=255)                                                                                                                              aGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGcc                          >  1:821719/1‑151 (MQ=255)                                                                                                                              aGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGcc                          >  1:92712/1‑151 (MQ=255)                                                                                                                                     cccccAGTTTATTGGTG‑AAAAAAAAACCGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGa                   <  2:998034/149‑1 (MQ=255)                                                                                                                                       cccAGTTCTTTGGTC‑CAAAAACAACCAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAAc                 <  1:431192/151‑1 (MQ=255)                                                                                                                                       cccAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAAc                 >  2:742572/1‑151 (MQ=255)                                                                                                                                         cAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGc               >  1:257156/1‑151 (MQ=255)                                                                                                                                               aTTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTg          >  1:668042/1‑150 (MQ=255)                                                                                                                                                ttGGGG‑CAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAg            <  2:850424/147‑1 (MQ=255)                                                                                                                                                    tG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGc     >  1:100166/1‑150 (MQ=255)                                                                                                                                                        gAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCAcc  >  1:532750/1‑150 (MQ=255)                                                                                                                                                        |                                                                                                                                                      CAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCC‑ATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAA‑CCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTG‑CGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCACC  >  NC_000913/1222448‑1222745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑