Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	50.9%	34.1 / 89.4	55	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (6/21);  new base T (28/0);  total (34/21)
Fisher's exact test for biased strand distribution p-value = 3.52e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.51e-08

CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206285‑3206585                                                                                                                                                       |                                                                                                                                                        cAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTc                                                                                                                                                         <  1:855665/151‑1 (MQ=255)            gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTcaaacaaaa                                                                                                                                                >  1:545469/1‑148 (MQ=255)                  cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAaaaaaaaaaa                                                                                                                                         >  1:275257/1‑150 (MQ=255)                   aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGTATTTTTCCAGACAAATACAAAAACt                                                                                                                                       <  1:47066/151‑1 (MQ=255)                     tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAcaaaaacaaaaaa                                                                                                                                        >  2:64475/1‑147 (MQ=255)                     tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAcatg                                                                                                                                     >  1:80877/1‑148 (MQ=255)                        cATTCCCTGCTCTGGCACAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGt                                                                                                                                  <  1:734722/151‑1 (MQ=255)                         aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGtt                                                                                                                                 <  2:908447/151‑1 (MQ=255)                         aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGtt                                                                                                                                 <  2:418536/151‑1 (MQ=255)                              cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATTGAGTTGCCa                                                                                                                             >  2:253519/1‑150 (MQ=255)                               tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAAAAAAAACAAAAAATGGGGTTGCCat                                                                                                                            >  1:764888/1‑150 (MQ=255)                                 ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATGTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatg                                                                                                                           <  1:747129/149‑1 (MQ=255)                                     ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAACACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgatg                                                                                                                        <  2:222197/148‑1 (MQ=255)                                          aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGGTGCCATGATG‑AGCGaaa                                                                                                                >  2:7041/1‑150 (MQ=255)                                          aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAAACAAAAAAAAAAAATGGAGGTGGCatggagggg                                                                                                                     >  1:494679/1‑140 (MQ=255)                                             attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATACAAAAAATGGAGTTGCCATGATG‑AGCGAAAGta                                                                                                             >  1:801255/1‑150 (MQ=255)                                              ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCGACAAAAAAAAAAAAAGGAGTTGCCATGATG‑AGGGGAAGaaa                                                                                                            >  2:645428/1‑150 (MQ=255)                                                cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATG‑AGCGAAAGtaa                                                                                                            >  2:660340/1‑148 (MQ=255)                                                 attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAAAAAAAAAAAAAATGGAGATGGCATGATG‑AAGGAAAAtaataa                                                                                                         >  1:5971/1‑150 (MQ=255)                                                 attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTCCACAAATACAAAAAATTGAGATGGCAAGAAG‑AGCGGAAGgaataa                                                                                                         >  2:851324/1‑150 (MQ=255)                                                    cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAAAACAAAAAATGGAGGTGCCATGATG‑AGCGAAAGGAAAAAACa                                                                                                      >  2:604245/1‑150 (MQ=255)                                                     ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATG‑AGCGAAAGAAAAAAGCaa                                                                                                     >  2:927036/1‑150 (MQ=255)                                                       aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACa                                                                                                   <  2:501166/150‑1 (MQ=255)                                                        ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGAGGTGCCATGATG‑AGCGAAAGAAATAAACaaaa                                                                                                   >  2:137606/1‑147 (MQ=255)                                                         tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCACACAAAAAAAAAAAATTGGGGTGTCATGGTG‑AGGGAAAGtgataaaaaa                                                                                                     >  1:198248/1‑142 (MQ=255)                                                            cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAAAAAAAAAAAAAAATGGAGGTTTCATGGTG‑AGGGAAAGtaaaaaaaaaaa                                                                                                   >  1:936402/1‑139 (MQ=255)                                                               gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGGCAAAAAAAAAAAATGGAGTTGCCATGATG‑AGGGAAAGAAATAAACAAAAAGCAggg                                                                                            >  2:122997/1‑147 (MQ=255)                                                                 tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                         <  1:442477/150‑1 (MQ=255)                                                                   aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAAAAAAAAAAAAGGAGGTGCCATGATG‑AGCGGAAGAAAAAAAAAAAAGGCAGGGAaaaa                                                                                       >  2:587256/1‑150 (MQ=255)                                                                     tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGt                                                                                     <  2:936764/150‑1 (MQ=255)                                                                         gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCCTGATG‑AGGGCAAATAAAAAGCAAAAGGCAGGGAATa                                                                                        >  2:46916/1‑143 (MQ=255)                                                                                 aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg                                                                         <  2:23873/150‑1 (MQ=255)                                                                                  aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc                                                                       <  1:497244/151‑1 (MQ=255)                                                                                   ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTACAACAAAAAAAAAACTGGAGTTGGCATGGTG‑AGGGAAAGGAATAAAAAACAGGCAGTGAaaaa                                                                                       >  1:291378/1‑134 (MQ=255)                                                                                      cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa                                                                   <  2:887267/151‑1 (MQ=255)                                                                                             aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAc                                                            <  1:713427/151‑1 (MQ=255)                                                                                               ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg                                                          <  1:703734/151‑1 (MQ=255)                                                                                                   gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAACAAAAAATGGAGTTTGCATGGTGAAGGGAAAG‑AATAACCAAAAAGCAATGAATAAATTTACAAAGATTGTCGCTAACCTTTCcccc                                                        >  1:875174/1‑149 (MQ=255)                                                                                                     aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATg                                                     <  2:545721/150‑1 (MQ=255)                                                                                                     aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTGGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGGGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATg                                                     <  1:627064/150‑1 (MQ=255)                                                                                                         aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGAGGTGCCCTGATG‑AGCGAAAAGAATAAACAAAAAGCAGTGAATAAGATGACAGAGATTTTTGCTAAATTTTCCCCCATGAttt                                                 >  2:74442/1‑150 (MQ=255)                                                                                                                  ccACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCAAGACAAATAAAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTAACAGAGATTGTCGCTAATTTTCCCGCCATGATTTCTACCCGAAt                                       <  1:490010/151‑1 (MQ=255)                                                                                                                   cacaTTTTAGGTGGTAGTTTGTGGCGATGATTTTTTCCAGACAAATACAAAAACAGGGGTTGCCAGGATG‑AGCGAAAGTAATAAGCAACAGGCAGTAAATATGTTGCAGGAGTTTGTCGCTATTTTTACCGCCATGATTTCTACCCGAATg                                      <  2:485142/151‑1 (MQ=255)                                                                                                                        ttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACAAAAACAAAAACTGGAGGTGGCATGGTG‑GGGGGAAGGAATAAAAAACAAGCAGTGAATAAGGTGACAGAGATTGTAGGTAACTTTACCGCCCTGGTTTTTTACCGAATGcc                                    >  1:102274/1‑148 (MQ=255)                                                                                                                        ttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGGCCTGATG‑AGCGGAAGTAATAAGCAAAAAGCAGTGAATAAGTTGACAGAGATTGTCGCTAAATTTACCGCCATGATTTTTACCCCAATGcc                                    >  2:854286/1‑148 (MQ=255)                                                                                                                           tAGTGGTAGTTTGTGGGGGTAAATTTTTCCAGACAAATACAAAAACTGGAGTGGCCATGATG‑AGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                               <  2:981596/150‑1 (MQ=255)                                                                                                                           tAGTGGTAGTTTGTGGCGGTGAGTTTTTTCCGCCAAACACAAAAAATGGAGGTTCCATGATG‑AGGGAAAGTAAAAAACAAAAGGGAGTGAAAAAGATGACAGAGGTTGTCGCTAACTTTACCCCCATGATTTTTTCCCGaaaacct                                   >  1:704133/1‑141 (MQ=255)                                                                                                                            aGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAACACAAAAACTGGGGGTGGCATGGTG‑GGGGGAAGGAAAAAACAAAACGCAGGGAAAAAAATGACAGAGAGTTTTGGTAAATTTAACCCCCCGATTTCTACCCCAAAGcc                                    >  1:162820/1‑144 (MQ=255)                                                                                                                                        tgGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                  >  2:231688/1‑150 (MQ=255)                                                                                                                                         ggCGGTAAATTTTTCCAGAAAAATACAAAAACTGGGGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc                <  2:471330/151‑1 (MQ=255)                                                                                                                                            ggTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa              >  2:559218/1‑150 (MQ=255)                                                                                                                                               gAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa           >  2:544608/1‑150 (MQ=255)                                                                                                                                                aaTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc          <  2:480369/150‑1 (MQ=255)                                                                                                                                                  tttttCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc       >  1:595077/1‑151 (MQ=255)                                                                                                                                                   ttttCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg        >  2:698024/1‑149 (MQ=255)                                                                                                                                                     ttCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa     >  1:748414/1‑150 (MQ=255)                                                                                                                                                       ccAGAAAAATACAAAAACTGGAGTTGCAATGATG‑AGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGCCGCTAACTTTACCGCCATGATTTCTACCCGAATGACTGATGACGTGGTGGATAAACTAAAACAGCTAAAg  <  2:80912/151‑1 (MQ=255)                                                                                                                                                       ccAGAAAAATAAAAAAACTGGATTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa    <  2:820232/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                        CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206285‑3206585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑