Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,443,277	0	A	C	42.2%	112.7 / 13.2	64	V274G (GTA→GGA)	secY	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (33/4);  new base C (0/27);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 3.72e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACT  >  NC_000913/3443128‑3443420                                                                                                                                                       |                                                                                                                                                cAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTAc                                                                                                                                                >  1:459102/1‑151 (MQ=255)  aGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTAcc                                                                                                                                               >  1:362592/1‑151 (MQ=255)    aCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTAcccc                                                                                                                                             <  2:814778/151‑1 (MQ=255)     cGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCTAAGATTGCCGGG‑ATTAccccc                                                                                                                                            <  1:375981/151‑1 (MQ=255)        aTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTCTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTACCGGG‑ATTACCCCCGcc                                                                                                                                         >  1:920595/1‑151 (MQ=255)          agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCat                                                                                                                                       >  1:1002641/1‑151 (MQ=255)          agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCat                                                                                                                                       >  2:983694/1‑151 (MQ=255)          agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCa                                                                                                                                        <  2:165104/150‑1 (MQ=255)          agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCa                                                                                                                                        >  1:440322/1‑150 (MQ=255)                  acaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACtt                                                                                                                               >  2:48472/1‑151 (MQ=255)                             ttccccgggcgaaaaaTACAGCAAAATTTTTGTCACCCAGTACCACCCATTACCCCCCCCGACCCATGCCGAGTTGTTCGCCGGAAAAAGAATAATCGGGGAAGCGAAAATGGCCGGG‑ATCCCCCCCGCCATATTCACTTTCAGCGGTaa                                                                                                                     <  2:891647/137‑1 (MQ=255)                              tGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                   >  2:564425/1‑151 (MQ=255)                                cccGGGCGGAAAATACAGCAAAATTTTTGTCAGCCAGTTCCACCCAGTCCCCCCCCCAACCCAGGACGGGAGGGTCGCCGGAAAAAAAATAATACGGGAAGCGAAGTTGGCGGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                   <  2:576713/149‑1 (MQ=255)                                cccAGGCTGAAAATACAGCAAAATTGTTGCCAGCCAGTCCCAACCAGTCCCCCCCCCGAACCAGGACGCGAGGGCCGCCGGAAACAAAAAAATACTGAAAGCAAAGATGGCCGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                   <  2:64608/149‑1 (MQ=255)                                  cAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCAAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                                                               >  1:161544/1‑151 (MQ=255)                                         cAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgt                                                                                                        >  2:12717/1‑151 (MQ=255)                                          aaaTACGGCAAAATTGTTGTCATCAACTCCAAACGAATCCCCCCCCCGAACGAGGACGTGATGGCCGCGGGAAAAAGAATAATACGGGAAGCGAATATTCCCGGA‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctct                                                                                                          <  2:418991/148‑1 (MQ=255)                                          aaaTACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgtg                                                                                                       >  1:768247/1‑151 (MQ=255)                                          aaaTACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgtg                                                                                                       >  1:887720/1‑151 (MQ=255)                                            aTACAGCGAATTTGTTGTCAGCCAGTTCCACCCAGTCCCCCCCCCGAACCAGGACGCGATGGTCGCCGGGAAAAAAATAATCCGGGAAGCAAAGATTGCGGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgca                                                                                                     <  2:853269/151‑1 (MQ=255)                                                 gagAATTTTTTGTCAGCCAGTTCCAACCAGTCCCGCCCCCGACCCAGGACGCGATGGTCGCCGGGAACAGAATAATCCTGAAAGCGAAGATTGCGGGG‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagca                                                                                                  <  2:402761/147‑1 (MQ=255)                                                     caTTGTTGGCAGCCAGTTCCAACCAGTACCGCCCCCGCACCATGACGCGATTGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                             >  1:705003/2‑150 (MQ=255)                                                     aaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                             >  1:903402/1‑150 (MQ=255)                                                      aTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                           >  2:980357/1‑151 (MQ=255)                                                       ttgtggCCAGCCGTTTCCACCCAGGCCCCCCCCCGACCAAGGACGCGTGGGCCGCCGGAAAAAAAATAATACTGAAAGCGAAGATGGCCGGT‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                           <  2:934544/150‑1 (MQ=255)                                                          ttgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg                                                                                       >  1:1021316/1‑151 (MQ=255)                                                          tggccAGCCAGTCCAACCCGGCCCCCCCCCCGACCCATGCCGGGATGGCCGCCGGAAACAAAATAATACGGGAAGCGAAGATTGCGGGG‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                        <  2:543670/146‑1 (MQ=255)                                                            gccacccagccccACCCAGTCCCCCCCCCGACCCATGGCGCGAGGTTCGCGGGGAACATAATAATAGTGGAGGCGAAGTTGGCCGGG‑AGCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACAcgacga                                                                                      <  1:414587/139‑1 (MQ=255)                                                                 ccAGTCCCAACCAGTCCCCCCCCCGACCAATGACGGGATGGCCGCCGGAAAAAAAAAAATCCTGGAAGCAAAGTTGGCGGGG‑TTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGc                                                                                <  2:523661/151‑1 (MQ=255)                                                                      tCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                           >  2:760827/1‑151 (MQ=255)                                                                      tCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                           >  1:861783/1‑151 (MQ=255)                                                                      cccACCCAGCACCCCCCCCGACCAAGGACGGGAGGGCCGCGGGAAACAGAAAAATACGGGAAGGGAAGATGGCCGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                           <  2:146105/150‑1 (MQ=255)                                                                       ccAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGt                                                                          >  2:454151/1‑151 (MQ=255)                                                                          aCCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                       >  2:117037/1‑151 (MQ=255)                                                                              gTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                   >  1:197564/1‑151 (MQ=255)                                                                                aCCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                   >  2:367362/1‑149 (MQ=255)                                                                                 ccccccccAAACCAGACCGCGATGGCCGCGGGAAAAAAAATAATACTGAAAGGAAAGATTGCGGGT‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTCGCTGACGTTTCGCGt                                                                   <  1:351562/145‑1 (MQ=255)                                                                                 ccGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGt                                                                >  2:64794/1‑151 (MQ=255)                                                                                 ccGCCCCCGAACCAAGACGCGAGGGCCCCCGGAAAAAGAAAAATTCGAGAAGCGAAGTTTGCCGTG‑TTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAg                                                                 <  1:164394/150‑1 (MQ=255)                                                                                       ccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAcc                                                           >  2:870570/1‑150 (MQ=255)                                                                                       ccGAACCAGGACGCGATGGTGGCGGGGAAAAAAATAATAGTGGAAGCGAGGTTTGCCGTT‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACca                                                          <  1:276447/151‑1 (MQ=255)                                                                                          aaCCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcaca                                                        >  1:107267/1‑150 (MQ=255)                                                                                            ccttgccgcgATGGCCGCGGGAAAAAAAATAATACTGAAGGCGAAGATTGCGGGT‑TTTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                      <  2:188703/144‑1 (MQ=255)                                                                                             caggACGCGATGGTCGCCGGAAACAAAATAATACTGAAAGCGAAGATTGCGGGT‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATg                                                     <  2:1023816/147‑1 (MQ=255)                                                                                              aTGGCGCGATGGTCCCGGGAAACAAAATAATTCTGGAAGGAAAAATTCCGGTT‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcg                                                   <  1:46109/151‑1 (MQ=255)                                                                                                     gATGGTCCCGGGAAAAAAAAAAATGCTGGAAGCGAAGTTTGCCGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                             <  2:1021691/150‑1 (MQ=255)                                                                                                        ggTCGCGGGGAACAAAATAATCCGGGAAGGAAATTTTGCGGGT‑TTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                      <  1:1004843/138‑1 (MQ=255)                                                                                                        ggTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAcc                                          >  1:633293/1‑150 (MQ=255)                                                                                                                 gAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaac                                 >  2:9933/1‑150 (MQ=255)                                                                                                                  aaaaaaataatACTGGAAGAAAAGATTGCCGGA‑TTTCCCTCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaacaa                               <  2:1004667/146‑1 (MQ=255)                                                                                                                   aCAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaacaa                               >  2:661879/1‑150 (MQ=255)                                                                                                                     aaaataatACGGGAAGAGAAGTTTGCGGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                            <  2:831120/149‑1 (MQ=255)                                                                                                                     aGAATAATACTGG‑AGGAAAGGTTCCCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                            <  1:728895/151‑1 (MQ=255)                                                                                                                     aGAATAATACGGGAAGCGAAGTTGGCCGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACaaa                              <  2:358814/149‑1 (MQ=255)                                                                                                                       aaaaataCGGGAAGCAAAGATTGCCGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                          <  2:997810/148‑1 (MQ=255)                                                                                                                              cTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                      >  1:145965/1‑148 (MQ=255)                                                                                                                              cTGAAAGCAAAGATTGCCGGT‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACaaa                     <  2:1010701/149‑1 (MQ=255)                                                                                                                                ggAAGCGAAGATTGCGGGG‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAg                    <  2:801691/148‑1 (MQ=255)                                                                                                                                    gaaaaGTTTGCGGGA‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt               <  1:674209/146‑1 (MQ=255)                                                                                                                                     cGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAATTACAACAAAGAACGTCAc            >  1:761121/1‑151 (MQ=255)                                                                                                                                          aTTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCa        >  1:106220/1‑150 (MQ=255)                                                                                                                                          aTTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCa        >  2:393477/1‑150 (MQ=255)                                                                                                                                              cggtg‑ATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat     <  1:371082/145‑1 (MQ=255)                                                                                                                                                ggg‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact  >  2:47503/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                CAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGG‑ATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACT  >  NC_000913/3443128‑3443420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑