Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,381,728 | 0 | A | C | 46.7% | 87.7 / 14.8 | 60 | G197G (GGT→GGG) | frdA | anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit |
Reads supporting (aligned to +/- strand): ref base A (29/3); new base C (0/28); total (29/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.36e-14 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGG > NC_000913/4381589‑4381878 | tcaGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGcc > 2:87976/1‑151 (MQ=255) caGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGcc < 2:583370/150‑1 (MQ=255) caGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGcc > 2:734940/1‑150 (MQ=255) ccgggCAGACCGGTTGGGTGATCCTGAACGAATCCCATGTCCCGCGGCGAAACGCCGTGGCTTAGCGCAATCCCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTTTAAGAATAACCGCGACCCGCACCGCCAGTAGCCATAACg < 1:478375/147‑1 (MQ=255) ccTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTCGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACg > 2:888668/1‑150 (MQ=255) gcccGGTTGGGTGATACTGAACGAATCCCATGCCACGCGGCGGAACGCCGGGGCTTAGCGCCATCCCCATCCCGTCCCCGGTACCGATGCCGCCGTGGGTGTTGTAACAAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGt < 1:123708/148‑1 (MQ=255) ccGTTTGGGTAATCCTGACCGAATCCCAGGCCCCGCGGCGAACCGCCGTGGCTTAGCGCCATACCCATCCCGCCCCCGTAACCGAGGCCGCCGTTGGTGTGGAAACGATAAACGCGCCCCGCTCCGCCAGTAGCCATAACGACcgc < 1:876537/146‑1 (MQ=255) ccGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGtt > 2:573494/1‑149 (MQ=255) ccGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGtt > 1:833947/1‑149 (MQ=255) ggTTGGGTGATACTGAACGAATCCCATGTCACGCAGCGGACCGCCGTGGCTTAGCGCCATCCCCATCCCGTCCCCGGTAACGATCCCGCCGTTGGTGTTGTAACGATAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCa < 1:967367/151‑1 (MQ=255) gtggggTGATACTGAACAATTTCCATTCCACGCGGCGGACCGCGGGGCTTTAGCGCCATCCCCACCCCGCCCCCGGTAACGATGCCGCCGTTGGTGTTGTAAGGAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACcgc < 1:403309/140‑1 (MQ=255) gggTGATACTGAACAAATTCCATTTCCCGCAGGGGAACGCGGGGGTTTGGCCCCATACCCATCCCGTCCCCGGAAACGATCCCGCCGTGGGTTTTGTAACAAAAAACGCGCCCCGCCCCGCCAGTAGCCATAACGACCGCGTTAGCACg < 2:609279/149‑1 (MQ=255) cTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCAcc > 1:567920/1‑151 (MQ=255) tGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCAcc > 1:358048/1‑150 (MQ=255) gAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCAcc > 2:1353/1‑149 (MQ=255) aaaaatgtCCGTGCCACGCAGGGAAACGCCGTGCCTTAGCCCCATCCCCACCCCGCCCCCGATACCGATGCCGCCGTTGGTGTTTTAACAAGAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCAcc < 1:401282/141‑1 (MQ=255) cGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGt > 1:477951/1‑151 (MQ=255) cGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCAGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCg > 1:764377/1‑150 (MQ=255) ccATGTCACGCAGCGGACCGCCGTGGTTTAGCGCCATCCCCATCCCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCtt < 2:186290/150‑1 (MQ=255) cGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATCCCGTCCCCGGAAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATg < 2:949696/150‑1 (MQ=255) gCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGtt > 1:469747/1‑151 (MQ=255) gCGGAACGCGGTGGCTTAGCGCCATCCCCATCCCGTCCCCGGTAACGATGCCGCCGTTGGTGTTGTAACGAAAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGtt < 2:375883/148‑1 (MQ=255) gCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCAt > 1:616332/1‑151 (MQ=255) acggcgggggtttACCGCCATACCCATCCCGTCCCCGGAACGGAGGCCGCGGTTGGTGTTGTAACGAAAAACGGGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGc < 1:427476/139‑1 (MQ=255) gTGGTTTAGCCCCATACCCATCCCGTCACCGATAACGATGCCGCCGTTGGTTTTGTAACAAAAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTAcc < 2:525990/148‑1 (MQ=255) tGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGc > 1:305600/1‑151 (MQ=255) cTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCcgc > 1:985449/1‑151 (MQ=255) cTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCcgc > 2:220909/1‑151 (MQ=255) ttAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCAGAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCACCACGTTCATTGCTACCAGGCcgcg > 1:498937/1‑151 (MQ=255) tAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCcgcg > 1:215438/1‑150 (MQ=255) aGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGa > 1:546829/1‑150 (MQ=255) cccctcccccaccccGTCCCCGGAAACGGTCCCCCTGTGGGTGTTGTAACGAAAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACa < 1:399642/138‑1 (MQ=255) cccatcccGTCACCGGTAACGAGGCCGCGGTTGGTGTTGTAACAATAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACcat < 2:928835/151‑1 (MQ=255) ccaatcccgcccccGGTACCGAGCCCGCCGTGGGTGTTGAAAGAAAAACCGCGCCCCGCCCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACa < 1:126390/132‑1 (MQ=255) ccaccccGTCCCCGGAACCGAGGCCGCCGTTGGTGTTTTAACGATAACCGCGCCCCGCCCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACcatc < 1:781857/146‑1 (MQ=255) ataccGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACcatcat > 2:236422/1‑151 (MQ=255) ccGTCCCGGGTAACGATGCCGCCGTTGGTGTTGTAACGAAAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCAtcatca < 2:492224/150‑1 (MQ=255) ccGTCCCCGGTAACGATGCCGCCTTGGGTGTTGTACCGATAAGCCCACCCCGCCCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCaa < 1:734634/151‑1 (MQ=255) cgggTAACGTTGCCCCCTTGGGGGTTGTAACGAAAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAg < 1:160197/147‑1 (MQ=255) ccGTTAACGGTCCCGCCGTGGGTGTTGAACCGAAAACCGCACCCCCCCCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGa < 1:760146/150‑1 (MQ=255) aaCGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATcc > 2:473059/1‑151 (MQ=255) aCGAGGCCGCCGTTGGTGTTGTAACGATAACCGCGCCCCGCGCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCa < 1:725020/151‑1 (MQ=255) gATGCCGCCGTTGTTGTTGTAACGAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAg < 2:987404/150‑1 (MQ=255) aTGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGc > 2:971973/1‑150 (MQ=255) ccgcctTTGGTGTTGTAACGATAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCa < 2:666946/148‑1 (MQ=255) cgTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATg > 1:634499/1‑151 (MQ=255) gtAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCaaa > 1:966505/1‑150 (MQ=255) gtAACGAAAACCGCGCCCCGCCCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCaaaa < 1:888321/151‑1 (MQ=255) tAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAAc > 1:646341/1‑151 (MQ=255) aactaaaaaCGCGCCCCCCCCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCaaaa < 2:175647/143‑1 (MQ=255) aaCGATAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCaaaa < 1:644908/149‑1 (MQ=255) aaCAATAAACGCCCCCCGCCCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAAc < 2:35372/150‑1 (MQ=255) aCGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGc > 2:479212/1‑151 (MQ=255) cGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCt > 1:881994/1‑151 (MQ=255) aaaaccgcgCCCCCCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTgg < 2:710178/146‑1 (MQ=255) aaaaaCCCGCCCGGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTg < 2:591100/148‑1 (MQ=255) aaaCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTg < 2:370618/148‑1 (MQ=255) aaaCGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACg > 1:308589/1‑145 (MQ=255) aaaCGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGa > 2:296903/1‑150 (MQ=255) aCGCGACCCGCACCGCCAGAAGCCACAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCt > 2:855175/1‑151 (MQ=255) aCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCggg > 1:34197/1‑151 (MQ=255) | TCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGG > NC_000913/4381589‑4381878 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |