Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,825,056	0	G	A	44.3%	96.7 / 41.9	79	intergenic (‑119/+84)	ves/spy	cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (39/5);  new base A (0/35);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 2.27e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAA  >  NC_000913/1824906‑1825201                                                                                                                                                         |                                                                                                                                                  aCCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTgag‑aagag                                                                                                                                                   <  2:720974/151‑1 (MQ=255)  ccGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTgag‑aagaga                                                                                                                                                  <  2:516516/151‑1 (MQ=255)  ccGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTgag‑aagaga                                                                                                                                                  <  2:821747/151‑1 (MQ=255)       aTTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGagaacaga                                                                                                                                             <  2:669973/151‑1 (MQ=255)                atatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAAccgccg                                                                                                                                    >  1:398211/1‑151 (MQ=255)                 tatCAAAGTATTCCATCCTCGCCCCCGGCCCGTTTTTTTCATTTTTATCGTTCAGTTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGCTTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAAAAAAccgccg                                                                                                                                    <  1:849373/150‑1 (MQ=255)                          aTTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGTTTTACGTTAGTGGTGATAAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTg                                                                                                                              >  2:513420/1‑147 (MQ=255)                                  cccGCCCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTACCCGTGGATGGTTATCGGCAACGGGGGTTTTTTACTTTAGTGGTGATCAGGTTTTTTTTACAACTT‑TTTGAG‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCa                                                                                                                     <  1:594022/146‑1 (MQ=255)                                  cTCGCCCCCGGCCCTTTCTTTTCTTTGTTATCGTTCAGTTACCCGTGGATGGTTATCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTG‑TTGGAG‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                    <  1:85109/149‑1 (MQ=255)                                   tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTAc                                                                                                                 >  2:138158/1‑151 (MQ=255)                                   tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTAc                                                                                                                 >  1:518641/1‑151 (MQ=255)                                    cccTCCCGCCCGGTTTTTTTCTTTTTTTTCGTTCAGGTAACCGTGGGTGGTTATCGGCACCTCGGGTGATTTACTTTGGGGGTTATCGGGTTTTTTTTACACCTG‑TTTGAA‑AAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                <  1:891898/149‑1 (MQ=255)                                     gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                               >  1:844267/1‑151 (MQ=255)                                     gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                               >  1:642346/1‑151 (MQ=255)                                     gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                               >  2:565614/1‑151 (MQ=255)                                     gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                               >  1:172325/1‑151 (MQ=255)                                     gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                               >  1:824704/1‑151 (MQ=255)                                     gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                               >  2:681666/1‑151 (MQ=255)                                      cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                              >  2:761275/1‑151 (MQ=255)                                      cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                              >  1:144509/1‑151 (MQ=255)                                        cccGGCCTGTTTTTTACTTTTTTATCGTTAAGGAAACCGTGGATGTTTATGGCCAACTGGGGTAATTTAGGTTGGTGGTAATCGGGTTTTTTTTACACTTG‑TTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                <  1:25613/147‑1 (MQ=255)                                         ccggccggTTCTTTTCATTGTTAGCCTGCAGGTACCCGTGAATGTTTATCGGCAACTCTGGTGATTTACCTTGGTGGGGATCAGGCTTTTTTTACTTCTG‑TGTGGG‑AAAAAAAAGGAAACCCGCCGATCCTGTCCACCGCAt                                                                                                                    <  2:298952/142‑1 (MQ=255)                                         ccGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAACGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                               >  2:931870/1‑147 (MQ=255)                                            acccttttttattaTTTTTATCTTTCAGATCACCGGGAATGGTGTGCGCCAACTCGGTTTATTTATGTTAGTGTTGATGGGGCTTTTTATAGAGCTG‑TTGGAG‑AAAAAAAAAGAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGt                                                                                                         <  1:696585/137‑1 (MQ=255)                                             cccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTTTCGGCAACGCGGGTAATTTACTTTAGTGTTGATCAGGTTTTTTTCCCACCTT‑TTTGAG‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg                                                                                                       <  2:745422/151‑1 (MQ=255)                                               cGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGC‑TTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                    >  1:454597/1‑151 (MQ=255)                                                gTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                    >  1:164050/1‑151 (MQ=255)                                                gTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                    >  1:928918/1‑151 (MQ=255)                                                 ttttttttttttttACCGTTCGGGTAACGGGGGATGTTTTTGGGAACCGCGGGTAATTAACGTTGGTGTTGATCAGGCTTTTTTCACAGCTT‑TTGAAG‑AGGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                     <  2:111042/137‑1 (MQ=255)                                                 ttcttttcttTGTTATCGTTCAGGTACCCGGGAATGTTTATCGCCACCTGGGGTAATTTACGTTGGTGTTGACCAGGTTTTTTTTACATCTG‑TTTGAA‑AAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                     <  2:89658/149‑1 (MQ=255)                                                 ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                    >  2:717767/1‑150 (MQ=255)                                                   cTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGCG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                  >  2:221608/1‑150 (MQ=255)                                                      ttctttttttttgTTCAGTTAACCGTGGATGTTTATCGGAAACTGGGGTGTTTTACGTTAGTGGTGCTCAGTCTTTTTTTACAACTT‑TTTGAA‑AAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg                                                                                               <  2:464738/138‑1 (MQ=255)                                                      ttagtttttATGGTTCAGGAACCCGGGGGTGGTTATCGGCAACTGGGGTGTTTTGTGTTGGTGGTGACCAGGTTTTTTTTACATGTT‑TTGAAA‑AAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg                                                                                               <  2:622319/143‑1 (MQ=255)                                                      ttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCACTACTGCAAGGTAGTGGACa                                                                                                 >  2:424014/1‑148 (MQ=255)                                                        cATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                            >  2:15739/1‑151 (MQ=255)                                                          tttttATCTTTCAGGTACCGGTGGATGGTTATCGGCAACTGGGGTGATTTACGTTGGGGGTGATCAGTTTTTTTTTACATCTG‑TTTGAA‑AAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                            <  1:641213/146‑1 (MQ=255)                                                          ttGTTTTCGTTCGGGTAACGGGGTTTTGTTATCCGCACCTGGGTTGATTTGCTTTAGGGGTGATCTGATTTTTTATACAAC‑G‑TTTTAGAAAGGAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg                                                                                               <  1:64588/146‑1 (MQ=255)                                                           tGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                         >  1:342767/1‑151 (MQ=255)                                                             ttttCGTGCGGGTACCCGTGTATGGTTACCGGAAACGGGGGTTTTTTAGGTTAGGGGTGATCGGGTTTTTTTCACAACTT‑TTTGAG‑AAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                         <  1:182335/146‑1 (MQ=255)                                                             ttATCGTTCGGGTAACGGTGGAGGTTTATCGCGACCTTGGGTGTTTTCCTTTAGTGTTAATCAGGTTTTTTTTAAATCTG‑TTGGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                         <  2:417387/149‑1 (MQ=255)                                                                 cGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                   >  2:849573/1‑151 (MQ=255)                                                                      aGGTAACCGGGGTTGTTTATCGGCAACTGGGTTGATTTACGTTAGGGGTGATCAGGTTTTTTTTACAACTG‑TTTGAG‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAg                                                                                <  1:716100/149‑1 (MQ=255)                                                                       ggTACCCGTGGATGGTTATCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAATTT‑TTTGAG‑AAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttt                                                                             <  1:765434/151‑1 (MQ=255)                                                                       ggTACCCGTGGATGGTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTG‑TTGGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGt                                                                               <  1:907911/149‑1 (MQ=255)                                                                          aaCGGTGGTTGGTTATCGGCAACTGGGTTGTTTTACTTTAGTGGTGATCAGCTTTTTTTTACAATTG‑TTGGAA‑AAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                          <  1:197794/151‑1 (MQ=255)                                                                          aaCCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                          >  1:218245/1‑151 (MQ=255)                                                                          aaCCGTGGATGGTTATCGCCAACGCGGGTGATTTCCGTTAGTGGTGAGCGGGCTTTTTTTCCACTTT‑TTGGAG‑AAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttt                                                                            <  2:792763/149‑1 (MQ=255)                                                                            ccGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg                                                                        >  1:346944/1‑151 (MQ=255)                                                                              gTGGTTGTTTATCGGCAACTGGGGTGATTTACTTTAGTGGTGATCAGGTTTTTTTTACAACTG‑TTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc                                                                       <  1:736725/150‑1 (MQ=255)                                                                               tGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAATTTTTTTTGCTg                                                                     >  2:583568/1‑151 (MQ=255)                                                                                      ttttgggCACCTCGGGGGATTTACGTTAGTGGTGATCAGTCTTTTTTTACAATTT‑TTTGAA‑AAAAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaa                                                                   <  2:189398/141‑1 (MQ=255)                                                                                       tATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTTTTAAGTTTTTTTGCTGAAAGatt                                                              >  2:804419/1‑150 (MQ=255)                                                                                           ggCAACTGGGTTGTTTTACTTTAGGGGTGATCAGGTTTTTTTTAC‑ACTGTTTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt                                                           <  1:909462/149‑1 (MQ=255)                                                                                           ggCAACTGGGGTGATTTACTTTGGTGGTGATCAGGTTTTTTTTACAACTT‑TTTGAG‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTc                                                          <  1:838599/150‑1 (MQ=255)                                                                                                   gggTAATTTACTTTAGTGGTGACCAGTTTTTTTTTACAACTG‑TTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTg                                                  <  1:58811/150‑1 (MQ=255)                                                                                                   gggTAATTTACGTTGGGGGTGAGAAGCTTTTTTTTACAACTT‑TTTAAA‑AAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt                                                   <  1:785131/149‑1 (MQ=255)                                                                                                     gTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGc                                                 >  1:139561/1‑149 (MQ=255)                                                                                                      tGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCa                                                >  1:565586/1‑149 (MQ=255)                                                                                                         tttACGTTGGTGGTGATCAGGTTTTTTTAACACCTT‑TTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAgg                                              <  1:406074/148‑1 (MQ=255)                                                                                                         ttaaCGTTAGTGGTGATCAGGTTTTTTTTACAACTG‑TTGGAA‑AAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa                                            <  1:345859/147‑1 (MQ=255)                                                                                                           tACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                         >  2:245229/1‑151 (MQ=255)                                                                                                             cGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                        >  1:544287/1‑150 (MQ=255)                                                                                                               tgaGTGGTGATCAGGTTTTTTTTACATCTG‑TTTGAG‑AAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAc                                      <  2:411944/148‑1 (MQ=255)                                                                                                                tAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAcc                                     >  1:897237/1‑150 (MQ=255)                                                                                                                 aGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                   >  1:512488/1‑151 (MQ=255)                                                                                                                  gtggtgATCAGTTTTTTTTTACAACTG‑TTGGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                   <  1:257056/150‑1 (MQ=255)                                                                                                                    ggtgACCAGTTTTTTTTAACACTTT‑TTGGAG‑AAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                   <  1:614847/148‑1 (MQ=255)                                                                                                                     gtgATCGGGGTTTTTTTACAACTT‑TTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTg                                <  1:793858/150‑1 (MQ=255)                                                                                                                     gtgATCAGGTTTTTTTTACAATTT‑TTTAAA‑AAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGc                               <  1:531821/151‑1 (MQ=255)                                                                                                                         tCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCg                             >  2:362867/1‑149 (MQ=255)                                                                                                                          cAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGAAGGCATTTTACCTTTTGCCGc                            >  1:549142/1‑149 (MQ=255)                                                                                                                          cAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTTAAGTTTTTTTGCTGGAAGATTATTCAGCAGTTGCAGGCACTTTACCTTTTGCCg                             >  2:133415/1‑148 (MQ=255)                                                                                                                           aGGTTTTTTTTAAAACTT‑TTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCt                           <  1:496636/149‑1 (MQ=255)                                                                                                                           aGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAACTTTTGCCGCTgg                         >  2:719166/1‑151 (MQ=255)                                                                                                                                 tttttACAATTG‑TTTGAA‑AAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                    <  1:940834/150‑1 (MQ=255)                                                                                                                                  ttttACAGCTG‑TTGGAA‑AAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTc                   <  1:401680/150‑1 (MQ=255)                                                                                                                                       cAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGt                >  1:542145/1‑148 (MQ=255)                                                                                                                                          cTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc           >  1:942149/1‑150 (MQ=255)                                                                                                                                           tG‑TTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc           <  2:772871/149‑1 (MQ=255)                                                                                                                                            g‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGc         >  1:445874/1‑150 (MQ=255)                                                                                                                                              tttGAG‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt        <  1:718185/150‑1 (MQ=255)                                                                                                                                              tttGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt        <  1:658050/150‑1 (MQ=255)                                                                                                                                               ttaag‑aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc           <  2:959022/143‑1 (MQ=255)                                                                                                                                                     aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaa  >  1:865034/1‑150 (MQ=255)                                                                                                                                                     aagaaaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt       <  1:117247/145‑1 (MQ=255)                                                                                                                                                     aagaaaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCa   <  2:298522/149‑1 (MQ=255)                                                                                                                                                         |                                                                                                                                                  ACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAA  >  NC_000913/1824906‑1825201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑