Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,825,061	0	G	A	40.7%	109.7 / 42.8	81	intergenic (‑124/+79)	ves/spy	cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (40/8);  new base A (0/33);  total (40/41)
Fisher's exact test for biased strand distribution p-value = 2.12e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCAT  >  NC_000913/1824912‑1825210                                                                                                                                                        |                                                                                                                                                      aTTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGagaacaga                                                                                                                                                      <  2:669973/151‑1 (MQ=255)          atatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAAccgccg                                                                                                                                             >  1:398211/1‑151 (MQ=255)           tatCAAAGTATTCCATCCTCGCCCCCGGCCCGTTTTTTTCATTTTTATCGTTCAGTTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGCTTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAAAAAAccgccg                                                                                                                                             <  1:849373/150‑1 (MQ=255)                    aTTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGTTTTACGTTAGTGGTGATAAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTg                                                                                                                                       >  2:513420/1‑147 (MQ=255)                            cccGCCCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTACCCGTGGATGGTTATCGGCAACGGGGGTTTTTTACTTTAGTGGTGATCAGGTTTTTTTTACAACTT‑TTTGAG‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCa                                                                                                                              <  1:594022/146‑1 (MQ=255)                            cTCGCCCCCGGCCCTTTCTTTTCTTTGTTATCGTTCAGTTACCCGTGGATGGTTATCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTG‑TTGGAG‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                             <  1:85109/149‑1 (MQ=255)                             tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTAc                                                                                                                          >  2:138158/1‑151 (MQ=255)                             tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTAc                                                                                                                          >  1:518641/1‑151 (MQ=255)                              cccTCCCGCCCGGTTTTTTTCTTTTTTTTCGTTCAGGTAACCGTGGGTGGTTATCGGCACCTCGGGTGATTTACTTTGGGGGTTATCGGGTTTTTTTTACACCTG‑TTTGAA‑AAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                         <  1:891898/149‑1 (MQ=255)                               gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                        >  2:565614/1‑151 (MQ=255)                               gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                        >  1:642346/1‑151 (MQ=255)                               gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                        >  2:681666/1‑151 (MQ=255)                               gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                        >  1:824704/1‑151 (MQ=255)                               gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                        >  1:844267/1‑151 (MQ=255)                               gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                        >  1:172325/1‑151 (MQ=255)                                cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                       >  2:761275/1‑151 (MQ=255)                                cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                       >  1:144509/1‑151 (MQ=255)                                  cccGGCCTGTTTTTTACTTTTTTATCGTTAAGGAAACCGTGGATGTTTATGGCCAACTGGGGTAATTTAGGTTGGTGGTAATCGGGTTTTTTTTACACTTG‑TTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                         <  1:25613/147‑1 (MQ=255)                                   ccggccggTTCTTTTCATTGTTAGCCTGCAGGTACCCGTGAATGTTTATCGGCAACTCTGGTGATTTACCTTGGTGGGGATCAGGCTTTTTTTACTTCTG‑TGTGGG‑AAAAAAAAGGAAACCCGCCGATCCTGTCCACCGCAt                                                                                                                             <  2:298952/142‑1 (MQ=255)                                   ccGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAACGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                        >  2:931870/1‑147 (MQ=255)                                      acccttttttattaTTTTTATCTTTCAGATCACCGGGAATGGTGTGCGCCAACTCGGTTTATTTATGTTAGTGTTGATGGGGCTTTTTATAGAGCTG‑TTGGAG‑AAAAAAAAAGAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGt                                                                                                                  <  1:696585/137‑1 (MQ=255)                                       cccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTTTCGGCAACGCGGGTAATTTACTTTAGTGTTGATCAGGTTTTTTTCCCACCTT‑TTTGAG‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg                                                                                                                <  2:745422/151‑1 (MQ=255)                                         cGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGC‑TTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                             >  1:454597/1‑151 (MQ=255)                                          gTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                             >  1:928918/1‑151 (MQ=255)                                          gTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                             >  1:164050/1‑151 (MQ=255)                                           ttttttttttttttACCGTTCGGGTAACGGGGGATGTTTTTGGGAACCGCGGGTAATTAACGTTGGTGTTGATCAGGCTTTTTTCACAGCTT‑TTGAAG‑AGGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                              <  2:111042/137‑1 (MQ=255)                                           ttcttttcttTGTTATCGTTCAGGTACCCGGGAATGTTTATCGCCACCTGGGGTAATTTACGTTGGTGTTGACCAGGTTTTTTTTACATCTG‑TTTGAA‑AAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                              <  2:89658/149‑1 (MQ=255)                                           ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                             >  2:717767/1‑150 (MQ=255)                                             cTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGCG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                           >  2:221608/1‑150 (MQ=255)                                                ttctttttttttgTTCAGTTAACCGTGGATGTTTATCGGAAACTGGGGTGTTTTACGTTAGTGGTGCTCAGTCTTTTTTTACAACTT‑TTTGAA‑AAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg                                                                                                        <  2:464738/138‑1 (MQ=255)                                                ttagtttttATGGTTCAGGAACCCGGGGGTGGTTATCGGCAACTGGGGTGTTTTGTGTTGGTGGTGACCAGGTTTTTTTTACATGTT‑TTGAAA‑AAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg                                                                                                        <  2:622319/143‑1 (MQ=255)                                                ttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCACTACTGCAAGGTAGTGGACa                                                                                                          >  2:424014/1‑148 (MQ=255)                                                  cATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                                     >  2:15739/1‑151 (MQ=255)                                                    tttttATCTTTCAGGTACCGGTGGATGGTTATCGGCAACTGGGGTGATTTACGTTGGGGGTGATCAGTTTTTTTTTACATCTG‑TTTGAA‑AAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                                     <  1:641213/146‑1 (MQ=255)                                                    ttGTTTTCGTTCGGGTAACGGGGTTTTGTTATCCGCACCTGGGTTGATTTGCTTTAGGGGTGATCTGATTTTTTATACAAC‑G‑TTTTAGAAAGGAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg                                                                                                        <  1:64588/146‑1 (MQ=255)                                                     tGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                                  >  1:342767/1‑151 (MQ=255)                                                       ttttCGTGCGGGTACCCGTGTATGGTTACCGGAAACGGGGGTTTTTTAGGTTAGGGGTGATCGGGTTTTTTTCACAACTT‑TTTGAG‑AAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                                  <  1:182335/146‑1 (MQ=255)                                                       ttATCGTTCGGGTAACGGTGGAGGTTTATCGCGACCTTGGGTGTTTTCCTTTAGTGTTAATCAGGTTTTTTTTAAATCTG‑TTGGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                                  <  2:417387/149‑1 (MQ=255)                                                           cGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                            >  2:849573/1‑151 (MQ=255)                                                                aGGTAACCGGGGTTGTTTATCGGCAACTGGGTTGATTTACGTTAGGGGTGATCAGGTTTTTTTTACAACTG‑TTTGAG‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAg                                                                                         <  1:716100/149‑1 (MQ=255)                                                                 ggTACCCGTGGATGGTTATCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAATTT‑TTTGAG‑AAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttt                                                                                      <  1:765434/151‑1 (MQ=255)                                                                 ggTACCCGTGGATGGTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTG‑TTGGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGt                                                                                        <  1:907911/149‑1 (MQ=255)                                                                    aaCGGTGGTTGGTTATCGGCAACTGGGTTGTTTTACTTTAGTGGTGATCAGCTTTTTTTTACAATTG‑TTGGAA‑AAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                                   <  1:197794/151‑1 (MQ=255)                                                                    aaCCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                                   >  1:218245/1‑151 (MQ=255)                                                                    aaCCGTGGATGGTTATCGCCAACGCGGGTGATTTCCGTTAGTGGTGAGCGGGCTTTTTTTCCACTTT‑TTGGAG‑AAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttt                                                                                     <  2:792763/149‑1 (MQ=255)                                                                      ccGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg                                                                                 >  1:346944/1‑151 (MQ=255)                                                                        gTGGTTGTTTATCGGCAACTGGGGTGATTTACTTTAGTGGTGATCAGGTTTTTTTTACAACTG‑TTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc                                                                                <  1:736725/150‑1 (MQ=255)                                                                         tGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAATTTTTTTTGCTg                                                                              >  2:583568/1‑151 (MQ=255)                                                                                ttttgggCACCTCGGGGGATTTACGTTAGTGGTGATCAGTCTTTTTTTACAATTT‑TTTGAA‑AAAAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaa                                                                            <  2:189398/141‑1 (MQ=255)                                                                                 tATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTTTTAAGTTTTTTTGCTGAAAGatt                                                                       >  2:804419/1‑150 (MQ=255)                                                                                     ggCAACTGGGTTGTTTTACTTTAGGGGTGATCAGGTTTTTTTTAC‑ACTGTTTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt                                                                    <  1:909462/149‑1 (MQ=255)                                                                                     ggCAACTGGGGTGATTTACTTTGGTGGTGATCAGGTTTTTTTTACAACTT‑TTTGAG‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTc                                                                   <  1:838599/150‑1 (MQ=255)                                                                                             gggTAATTTACTTTAGTGGTGACCAGTTTTTTTTTACAACTG‑TTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTg                                                           <  1:58811/150‑1 (MQ=255)                                                                                             gggTAATTTACGTTGGGGGTGAGAAGCTTTTTTTTACAACTT‑TTTAAA‑AAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt                                                            <  1:785131/149‑1 (MQ=255)                                                                                               gTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGc                                                          >  1:139561/1‑149 (MQ=255)                                                                                                tGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCa                                                         >  1:565586/1‑149 (MQ=255)                                                                                                   tttACGTTGGTGGTGATCAGGTTTTTTTAACACCTT‑TTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAgg                                                       <  1:406074/148‑1 (MQ=255)                                                                                                   ttaaCGTTAGTGGTGATCAGGTTTTTTTTACAACTG‑TTGGAA‑AAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa                                                     <  1:345859/147‑1 (MQ=255)                                                                                                     tACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                                  >  2:245229/1‑151 (MQ=255)                                                                                                       cGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                                 >  1:544287/1‑150 (MQ=255)                                                                                                         tgaGTGGTGATCAGGTTTTTTTTACATCTG‑TTTGAG‑AAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAc                                               <  2:411944/148‑1 (MQ=255)                                                                                                          tAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAcc                                              >  1:897237/1‑150 (MQ=255)                                                                                                           aGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                            >  1:512488/1‑151 (MQ=255)                                                                                                            gtggtgATCAGTTTTTTTTTACAACTG‑TTGGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                            <  1:257056/150‑1 (MQ=255)                                                                                                              ggtgACCAGTTTTTTTTAACACTTT‑TTGGAG‑AAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                            <  1:614847/148‑1 (MQ=255)                                                                                                               gtgATCGGGGTTTTTTTACAACTT‑TTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTg                                         <  1:793858/150‑1 (MQ=255)                                                                                                               gtgATCAGGTTTTTTTTACAATTT‑TTTAAA‑AAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGc                                        <  1:531821/151‑1 (MQ=255)                                                                                                                   tCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCg                                      >  2:362867/1‑149 (MQ=255)                                                                                                                    cAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGAAGGCATTTTACCTTTTGCCGc                                     >  1:549142/1‑149 (MQ=255)                                                                                                                    cAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTTAAGTTTTTTTGCTGGAAGATTATTCAGCAGTTGCAGGCACTTTACCTTTTGCCg                                      >  2:133415/1‑148 (MQ=255)                                                                                                                     aGGTTTTTTTTAAAACTT‑TTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCt                                    <  1:496636/149‑1 (MQ=255)                                                                                                                     aGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAACTTTTGCCGCTgg                                  >  2:719166/1‑151 (MQ=255)                                                                                                                           tttttACAATTG‑TTTGAA‑AAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                             <  1:940834/150‑1 (MQ=255)                                                                                                                            ttttACAGCTG‑TTGGAA‑AAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTc                            <  1:401680/150‑1 (MQ=255)                                                                                                                                 cAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGt                         >  1:542145/1‑148 (MQ=255)                                                                                                                                    cTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc                    >  1:942149/1‑150 (MQ=255)                                                                                                                                     tG‑TTTGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc                    <  2:772871/149‑1 (MQ=255)                                                                                                                                      g‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGc                  >  1:445874/1‑150 (MQ=255)                                                                                                                                        tttGAG‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt                 <  1:718185/150‑1 (MQ=255)                                                                                                                                        tttGAA‑AAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt                 <  1:658050/150‑1 (MQ=255)                                                                                                                                         ttaag‑aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc                    <  2:959022/143‑1 (MQ=255)                                                                                                                                               aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaa           >  1:865034/1‑150 (MQ=255)                                                                                                                                               aagaaaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt                <  1:117247/145‑1 (MQ=255)                                                                                                                                               aagaaaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCa            <  2:298522/149‑1 (MQ=255)                                                                                                                                                  aaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTCTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAt        <  1:251658/148‑1 (MQ=255)                                                                                                                                                    aaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAg     <  2:227404/151‑1 (MQ=255)                                                                                                                                                      aagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGc    >  2:5451/1‑150 (MQ=255)                                                                                                                                                       agaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCAt  <  1:318707/151‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                      ATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTG‑TTTGAG‑AAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCAT  >  NC_000913/1824912‑1825210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑