Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,481,804	0	G	A	38.0%	98.6 / 34.9	71	P125S (CCA→TCA)	emrY	putative multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base G (33/11);  new base A (0/27);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 4.65e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

ATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGT  >  NC_000913/2481654‑2481946                                                                                                                                                           |                                                                                                                                               atatCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTgg                                                                                                                                                <  1:958120/151‑1 (MQ=255)   atCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGa                                                                                                                                               <  1:211044/150‑1 (MQ=255)     ccAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAAtt                                                                                                                                            <  2:24353/151‑1 (MQ=255)     ccAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAAtt                                                                                                                                            >  2:802457/1‑151 (MQ=255)       aaCCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGACTTaa                                                                                                                                          <  2:273653/151‑1 (MQ=255)        aCCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAAc                                                                                                                                         >  1:217882/1‑151 (MQ=255)         ccccAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACg                                                                                                                                        >  1:5511/1‑151 (MQ=255)            cAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGcc                                                                                                                                     <  2:381336/151‑1 (MQ=255)                 aaaGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGcc                                                                                                                                     >  2:464506/1‑146 (MQ=255)                 aaaGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCg                                                                                                                                  <  2:495469/149‑1 (MQ=255)                  aaGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGCGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGcc                                                                                                                                >  1:406457/1‑150 (MQ=255)                  aaGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCa                                                                                                                               <  2:680308/151‑1 (MQ=255)                     ttATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCAtt                                                                                                                             >  2:628488/1‑150 (MQ=255)                              atatAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGACATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCACTGa                                                                                                                    >  2:793754/1‑150 (MQ=255)                                    cccccaaaTCTCGGCCCAAATATGGGAG‑GATAATGCTCGTTATTTGACAATATTGCGAGACCAAATTTTCTTTTTCCTGGGGTATATTTCTTT‑AAAAAAAAT‑CTCTGT‑GACAGTGAAATTAGCGGCCCCGCCATTAACCCCTGAACGActc                                                                                                             <  1:868732/145‑1 (MQ=255)                                    ccGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGActc                                                                                                             >  1:796371/1‑151 (MQ=255)                                    ccGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGActc                                                                                                             >  1:424950/1‑151 (MQ=255)                                        ccAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTaa                                                                                                          >  1:656046/1‑150 (MQ=255)                                          aaTACCGCCCCACATAGCGGAGCAAAAAT‑CACGGTCTTTGCCAAAAATGCCAGAAAAATTTTTTTTTTTTCGGGGGAATATTTCTTA‑AAAAAAAAC‑CTCGGG‑AACAGGGAAATAAACGGCCCCGCCATTAACCCCTGAACGACTCTaaaaa                                                                                                       <  1:481295/151‑1 (MQ=255)                                           atatCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTaaaaa                                                                                                       >  1:622072/1‑150 (MQ=255)                                                ggCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATc                                                                                                  >  2:283518/1‑150 (MQ=255)                                                ggCCCACATATCGGAGCAATAAT‑CCCGGTCATTGACCATAATGCCAGAGAAAATGTTTTTTTTTCGGGCGGATAATTCTTA‑AAAAATAAC‑CTCTGT‑GAAAGTGAAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAata                                                                                                    <  1:908257/148‑1 (MQ=255)                                                            ggAGGGATAAT‑CACGTTCATTGCCCAAATGGCAAGAGAAATTGTTTTTTTTTCTGGGGTATATTCCCTA‑AAAAATAAA‑CTCTGT‑AAAAGTAAAATAAGCGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCa                                                                                                 <  2:56911/139‑1 (MQ=255)                                                              gggaaaaaatcggggTAATTGACAAAAACGCAAGAGAAAATGTTCTTTTTCCTGGCGGATAATTCCTT‑AATAAAAAA‑CTCGGA‑GAAAGAAAAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGAtt                                                                                    <  2:723612/138‑1 (MQ=255)                                                                 gaaaaT‑CGCGCTCATTGACCTAAGTGCTAGAGAAAATGTTCTTTTTTGTGGTGTAAAACTCCTT‑AACAATAAT‑CCGGGT‑GACGGGGGAATAAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTg                                                                                   <  1:464786/145‑1 (MQ=255)                                                                    aaT‑CACGTTCATTGACAATAATGCCAAAGAAAATTTTTTTTTTTCGGGGGAATAATTCCTA‑AAAAATAAC‑CTCTGT‑GACAGTGGAATAAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGt                                                                                 <  1:933116/147‑1 (MQ=255)                                                                    aaT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGa                                                                              >  2:508440/1‑150 (MQ=255)                                                                        ctggttCATTGACCATAAAGCCAGAGAAAATTTTTTTTTTTCGGGCGGATAATTCTTA‑AAAAAAAAA‑CTCTGT‑GACGGGGAAATTAGCGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATa                                                                            <  1:9800/144‑1 (MQ=255)                                                                        cACTGTCATTAACCATAATGCCAGAGCAAATTTTTTTTTTTCTGGCGGATATTCCATT‑AATAATAAA‑CTGTTT‑GACAGAGAAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAg                                                                           <  2:902149/150‑1 (MQ=255)                                                                           ggTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGACAg                                                                           >  2:595888/1‑147 (MQ=255)                                                                            gTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTac                                                                      >  1:876718/1‑151 (MQ=255)                                                                              cATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTacac                                                                    >  1:899939/1‑151 (MQ=255)                                                                                ttGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACAt                                                                  >  1:223744/1‑151 (MQ=255)                                                                                       tAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGa                                                            >  1:436167/1‑150 (MQ=255)                                                                                        attGCCAGAAAAAATTTTTTTTTTTCGGGCGAATATTTCATT‑AAAAAAAAA‑CT‑TGTAGAAAGTGAAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGaa                                                           <  2:339905/148‑1 (MQ=255)                                                                                        aaTGCCAGAGAAATTTTTTTTTTTTCGGGGGAATAATTCTTA‑AAAAAAAAC‑CTCTGT‑GACAGTGAAATAAACGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAg                                                          <  1:941197/151‑1 (MQ=255)                                                                                        aaTGCCAAACAAAATTTTCTTTTTT‑TGGCCGATAATTTCTTAAATAATAAC‑CTTGTT‑AACAGTAAAATTACCGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAg                                                          <  1:802910/151‑1 (MQ=255)                                                                                            caagacaaattcttCTTTTTTCGGGCGGAAAATTCTTA‑AAAAAAAAA‑CTCTTA‑GAAAGTGAAATTAGCGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACa                                                       <  2:587366/138‑1 (MQ=255)                                                                                              agaaCAAATTTGCTTTTTTCGGGCGGATAATTCATA‑AAAAAAAAA‑CTCGGA‑AAAATTAAAATAACCCGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAg                                                      <  2:530168/149‑1 (MQ=255)                                                                                               gagCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCg                                                    >  1:605384/1‑150 (MQ=255)                                                                                               agagaaaTTTTTTTTTTTTCGGGGGAAAATTCCTT‑AAAAAAAAACCTCTGT‑AAAAGTGAAATAAAGGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACa                                                       <  1:72781/144‑1 (MQ=255)                                                                                                agCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCAGCCATTAACCCTTGAACGACTCTAAAAAATATCAGCACATAAAGATTGGTCGATAGGCAACACATTAATGAAGACAGCGa                                                   >  2:67075/1‑150 (MQ=255)                                                                                                   aaaTTTTTTTTTTTCGGGCGGATATTTCCTA‑AAAAAAAAC‑CTCTGT‑GAAAGGGAAATTAACGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaaa                                               <  1:676087/151‑1 (MQ=255)                                                                                                    attttttttttttCTGGCGATTATTTCTTA‑AAAAAAAAA‑CTCGGA‑GAAAGAAGAATCAGCCCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGTTCGATAGGCTACACATTAATGAAGACAGCGaaa                                                 <  2:686605/141‑1 (MQ=255)                                                                                                      tGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaaaaa                                             >  2:280801/1‑150 (MQ=255)                                                                                                        tttttttttGTGGCGGATATTTCTTA‑AAAAAAAAA‑CTCTGT‑AAAAGTGAAATAAACGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaa                                                <  2:144694/142‑1 (MQ=255)                                                                                                         ttttttttCTGGCGAATATTTCTTA‑AAAAATAAC‑CTCTGT‑GACAGTGAAATTACCGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGt                                          <  1:863502/148‑1 (MQ=255)                                                                                                            tttttCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACt                                      >  1:937031/1‑151 (MQ=255)                                                                                                             ttttCGGGCGGATAATTCCTA‑AAAAAAAAC‑CTCTGT‑GAAAGTGAAATAAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACt                                      <  1:847600/150‑1 (MQ=255)                                                                                                                tCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGaa                                   >  1:899034/1‑150 (MQ=255)                                                                                                                 ggggagaaaaaTTCTTA‑AAAAAAAAA‑CCGTGT‑GAAAGTAAAATAACCGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGaa                                   <  2:783076/140‑1 (MQ=255)                                                                                                                   gggggAAAATTCCTA‑AAAAAAAAC‑CTCTGT‑GAAAGGGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGaa                                   <  1:338955/144‑1 (MQ=255)                                                                                                                   ggCGGATATTTCCTT‑AATAATAAC‑CTCTGT‑GACAGTGAAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGaaa                                  <  1:314844/148‑1 (MQ=255)                                                                                                                   ggCGAATAATTCCTT‑AATAACAAA‑CTCTGT‑AACAGTAAAATAAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCGCATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTa                               <  2:731554/151‑1 (MQ=255)                                                                                                                   ggCGAATAATTCCTA‑AAAAATAAC‑CTTTGT‑GACAGTAAAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCG‑AAAAAAAGTGACTGAAAGt                                <  2:164752/149‑1 (MQ=255)                                                                                                                    gcgaaaaaTTCGTA‑AAAAATAAA‑CTCTGG‑AAAAGGAAAATAAACCGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTaa                              <  2:458703/145‑1 (MQ=255)                                                                                                                     cGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTaa                              >  2:860818/1‑150 (MQ=255)                                                                                                                      ggATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTaaa                             >  1:532551/1‑150 (MQ=255)                                                                                                                         tAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAaat                          >  1:213601/1‑150 (MQ=255)                                                                                                                         tAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAaat                          >  1:742492/1‑150 (MQ=255)                                                                                                                         tAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCCACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAaat                          >  2:836253/1‑150 (MQ=255)                                                                                                                                tt‑AATAATAAC‑CTCTGT‑GACAGGGAAATAAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCtt                    <  1:406535/149‑1 (MQ=255)                                                                                                                                tt‑AATAAAAAC‑CCCTGT‑GAAAGTAAAATTAAGGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTa                   <  2:86030/150‑1 (MQ=255)                                                                                                                                     taataaA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCg              >  2:726444/1‑151 (MQ=255)                                                                                                                                      aataaA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGc             >  1:669982/1‑151 (MQ=255)                                                                                                                                      aataaA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGc             >  2:91479/1‑151 (MQ=255)                                                                                                                                         aaa‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCt           >  1:423551/1‑150 (MQ=255)                                                                                                                                               ctGT‑GAGAGTGGAAGTCACGGCTCCGCCATTAACCCATGAACGACTCTAAAAACTATCAGCAAATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGTAAGTAAAAATAATCTTAATTCGCCTATTCtt     >  2:806192/1‑151 (MQ=255)                                                                                                                                                 gt‑gACAGTGAAATTACCGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTtg   <  2:664398/151‑1 (MQ=255)                                                                                                                                                     aaaGTGAAATTAGCTGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTAt         <  2:834990/140‑1 (MQ=255)                                                                                                                                                     aaaGTGAAATAACCGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTtgt  <  2:424757/147‑1 (MQ=255)                                                                                                                                                      cAGGGGAATAAAGGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTtgt  <  1:88962/148‑1 (MQ=255)                                                                                                                                                           |                                                                                                                                               ATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAAT‑CACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTT‑AATAATAAA‑CTCTGT‑GACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGT  >  NC_000913/2481654‑2481946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑