Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT38.2% 74.9 / 26.2 55intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (8/26);  new base T (21/0);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 5.18e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206293‑3206584
                                                                                                                                                 |                                                                                                                                                       
ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACaa                                                                                                                                                  <  2:407150/150‑1 (MQ=255)
      aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaa                                                                                                                                           <  2:303976/151‑1 (MQ=255)
             gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGCCAAATACaaaa                                                                                                                                          >  2:955466/1‑145 (MQ=255)
              ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCAAAcaaaaacaaaaaatag                                                                                                                                     >  2:301004/1‑145 (MQ=255)
                  tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGtt                                                                                                                                 >  1:150297/1‑149 (MQ=255)
                      tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACCAAAACAAAAAATGGAGTTggc                                                                                                                              >  2:257225/1‑146 (MQ=255)
                       gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatg                                                                                                                           <  1:172256/150‑1 (MQ=255)
                         tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGAGGTGCAATTTTTCCGACTAAAACAAAAAACGGAGGTgggatta                                                                                                                          >  2:281712/1‑143 (MQ=255)
                          ctGGCGCAATATTCATTCTTATTTTCGCGTTGAATCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAGACAAAAACAAAAACTGGGGTTGTCatga                                                                                                                          >  2:532804/1‑148 (MQ=255)
                             gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAtgatga                                                                                                                       <  2:953386/148‑1 (MQ=255)
                                cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATTGGGTTGCCCTGATGAGCgga                                                                                                                  >  1:830584/1‑148 (MQ=255)
                                    attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaa                                                                                                             <  2:348410/151‑1 (MQ=255)
                                       cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaata                                                                                                          <  1:782920/150‑1 (MQ=255)
                                        attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAg                                                                                                        <  1:495308/151‑1 (MQ=255)
                                         ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATACAAAAAATGGAGTTGGCATGATGAGCGGAAGaaataa                                                                                                         >  1:153567/1‑149 (MQ=255)
                                             tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGGAGTTTGTGGCGGTGGATTTTTTCCCACCAATAAAAAAAAAGGAGGTTCCATGAGGAGCGGAAAAAATAAACaa                                                                                                     >  1:587847/1‑149 (MQ=255)
                                              aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGAGCGGAAATAAAAAAAAACa                                                                                                  >  1:852011/1‑150 (MQ=255)
                                                 ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                               <  2:748693/150‑1 (MQ=255)
                                                 ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAATAAAAAATGGGGGTGTCATGGTGAGGGaaaagaaaaaaaa                                                                                                       >  2:10296/1‑134 (MQ=255)
                                                  tctcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTGTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                              <  2:757352/147‑1 (MQ=255)
                                                        tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                        <  1:685302/150‑1 (MQ=255)
                                                         gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                      <  2:14959/151‑1 (MQ=255)
                                                         gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                       <  2:2517/150‑1 (MQ=255)
                                                            tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGt                                                                                    <  1:24741/150‑1 (MQ=255)
                                                              aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGa                                                                                 <  1:900648/151‑1 (MQ=255)
                                                                 aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTCAACAAAAAAAAAAAACGGAGTTGTCATGATGAGGGGAAGGAAAAAACAACAAGCAGTGGAAAAATTggaa                                                                               >  1:629543/1‑147 (MQ=255)
                                                                       tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAtt                                                                         <  2:958237/150‑1 (MQ=255)
                                                                        aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGt                                                                       <  1:364121/151‑1 (MQ=255)
                                                                        aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAAACAAAAACAAAAAAAGGAGTTGGCATGATGAGCGAAAGAAATAAAAAAAAAACAGGGAATAAATTGAAAgaga                                                                           >  2:856181/1‑147 (MQ=255)
                                                                        aaTTACATATTCACGGTGGCAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGt                                                                       <  1:276463/150‑1 (MQ=255)
                                                                         aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTCACACAAAAACAAAAACAGGGGCTGCTATGTTGAGTGGAAGTAATAAAGCAAAAGAAGTGAATaaagttcccgagatt                                                                         >  2:716658/1‑135 (MQ=255)
                                                                                atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAAc                                                                <  1:735131/150‑1 (MQ=255)
                                                                                  tCACGGTGGCAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGCGGCGGGGAACTTTTTTAAAACAAAACAAAAAAAGGAGTTTGCATGATGAGCGAAAGTAATAAACAACAGCAAGAGTAAAAATGTACAGAGATTgaggctcaactt                                                             >  2:520790/1‑140 (MQ=255)
                                                                                  tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAACTGGAGTTGTCATGATGAGCGAAAATAATAAACAAAAAGCAGGGAATAAATTGACAGAGAATGTCGCTAAAttt                                                             >  1:607308/1‑151 (MQ=255)
                                                                                  tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt                                                              <  1:239981/150‑1 (MQ=255)
                                                                                      ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg                                                         <  2:331576/151‑1 (MQ=255)
                                                                                       gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAAAAAAAAAAATGGAGTTGCCATGATGAGCGAAAGGAAAAAACAAAAAGCAGGGAAAAAATTGACAGAGAGTTTCGCTAACTTTTCCGc                                                        >  1:467919/1‑151 (MQ=255)
                                                                                        tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGAAAGGAATAAGCAAAAAGCAGTGGATAAGTTGACAGAGAATGTCGCTAACTTTACCGcc                                                       >  1:599826/1‑151 (MQ=255)
                                                                                            aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATg                                                    <  1:286953/150‑1 (MQ=255)
                                                                                              aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAtt                                                 <  1:48562/151‑1 (MQ=255)
                                                                                                aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCAAACAAAAACAAAAAATGGAGTTGTCATGATGAGCGAAAGTAATAAAAAACAGGAAGAGAAAAAATTGACAGAGATTGTCGCTAACTTTACCGCCATGAttt                                                >  2:677662/1‑150 (MQ=255)
                                                                                                   ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAATTGAGTTGGCCTGATGGGCGGAAGGAATAAGCAAAAGGCAGTGAATAAGTTGAAAGAGATTGTTGCTAAATTTAACCCCCAGAttttt                                              >  2:893007/1‑147 (MQ=255)
                                                                                                          cacaTTTTTAGTGGTATTTTGTGGTGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATg                                     <  1:774086/151‑1 (MQ=255)
                                                                                                          cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGGGGTGGCATGATGAGCGAAAGGAATAAACAAAAGGCAGTGGAAAAATTGACAGAGAATGTCGCTAACTTTTCCGCCCTGATTTTTACCCCaa                                       >  1:398543/1‑149 (MQ=255)
                                                                                                           acaTTTTTAGTGGTAGTTTGTGGCGGGGACTTTTTTCCGACAAAAACAAAAAATTGAGGTGCCATGATTAGGGAAAAGAATAAACAAAAGGCAGGGAATAAAATGACAGAAATTTTCCCTAAACTTAACCCCATGGTTTTTACCCCAATg                                     >  1:869705/1‑150 (MQ=255)
                                                                                                                  tAGTGGTAGTTTGTGGCGGTGGAATTTTTCCGACAAATAAAAAAAATGGAGTTTGCCTGATGAGGGGAAGTAATAAACAAAAGGGAGAGAAAAAATTTAAAGAGATTTTTGCTAAATTTTCCCCCCCGATTTTTAACCCaa                                       >  2:250886/1‑141 (MQ=255)
                                                                                                                    gTGGTAGTTTGTGGCGGTGTATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTAACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGGCg                           <  2:672659/151‑1 (MQ=255)
                                                                                                                    gTGGTAGTTTGTGGCGGCGAATTTTTCCAGACAAATACAAAAACAGGAGGTGCCATGATTGGGGAAAGGAACAAGAAACAGGCAGGGAAACAGAGGACAGAGATTGTGGCGTACTTTTCCGCCGTGCGTTTTTCCCTCATGCCtggtg                              >  2:770726/1‑148 (MQ=255)
                                                                                                                      ggTAGTTTGTGGCGGTGAATTTTTCCAGACCAAAAAAAAAACTGGAGGTGGCCTGGTGAGGGAAAATAATAAAGAACAAGGCGGGAAAAAAATTGAAGAGAATGTCGGCAACTTTACCCCCCTGATTTCTAACCGAAAGCCCGATGAAgtg                         >  2:538487/1‑151 (MQ=255)
                                                                                                                           tttGTGGTTGTAAATTTTTCTAGACAAATTCAAATACTGGATTTGCCATTATGGGCGAAATTAATAGCCAACGGGGTGTTAATAAGTTTACAGAGTTGGACACTCGCTTTCCCGCCATGATTTCTACCCGAATGCCtgatga                             <  2:184690/142‑1 (MQ=255)
                                                                                                                               tgGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                 >  1:74222/1‑150 (MQ=255)
                                                                                                                                ggCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc               >  1:694017/1‑151 (MQ=255)
                                                                                                                                   ggTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa             >  2:381627/1‑150 (MQ=255)
                                                                                                                                    gTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa            >  1:920754/1‑150 (MQ=255)
                                                                                                                                         aaTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc         <  1:303054/150‑1 (MQ=255)
                                                                                                                                                 ccAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa  <  1:849870/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                       
TTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206293‑3206584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: