Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,206,435 | 0 | C | T | 38.2% | 74.9 / 26.2 | 55 | intergenic (‑179/‑28) | ttdR/ttdA | transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit |
Reads supporting (aligned to +/- strand): ref base C (8/26); new base T (21/0); total (29/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.18e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA > NC_000913/3206293‑3206584 | ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACaa < 2:407150/150‑1 (MQ=255) aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACaaa < 2:303976/151‑1 (MQ=255) gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTTCAGCCAAATACaaaa > 2:955466/1‑145 (MQ=255) ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑G‑AA‑ATTTTTCAAAcaaaaacaaaaaatag > 2:301004/1‑145 (MQ=255) tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTTCAGACAAAAAAAAAAAATGGAGtt > 1:150297/1‑149 (MQ=255) tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GA‑TTTTTTCAAACCAAAACAAAAAATGGAGTTggc > 2:257225/1‑146 (MQ=255) gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCatg < 1:172256/150‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGAGGT‑GCAA‑TTTTTCC‑GACTAAAACAAAAAACGGAGGTgggatta > 2:281712/1‑143 (MQ=255) ctGGCGCAATATTCATTCTTATTTTCGCGTTGAATCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑G‑AA‑TTTTTTCAGACAAAAACAAAAACTGGGGTTGTCatga > 2:532804/1‑148 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCAtgatga < 2:953386/148‑1 (MQ=255) cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTTCAGACAAAAACAAAAAATTGGGTTGCCCTGATGAGCgga > 1:830584/1‑148 (MQ=255) attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaa < 2:348410/151‑1 (MQ=255) cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat‑a < 1:782920/150‑1 (MQ=255) attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAg < 1:495308/151‑1 (MQ=255) ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTTCAGAAAAATACAAAAAATGGAGTTGGCATGATGAGCGGAAGaaat‑aa > 1:153567/1‑149 (MQ=255) tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGGAGTTTGTGGCGGT‑G‑GA‑TTTTTTCCCACCAATAAAAAAAAAGGAGGTTCCATGAGGAGCGGAAAAAAT‑AAACaa > 1:587847/1‑149 (MQ=255) aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGAGCGGAAATAAA‑AAAAAAC‑a > 1:852011/1‑150 (MQ=255) ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGc < 2:748693/150‑1 (MQ=255) ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑ATTTTTCAGAAAAAAATAAAAAATGGGGGTGTCATGGTGAGGGaaaagaaaaaaaa > 2:10296/1‑134 (MQ=255) tctcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTGTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCa < 2:757352/147‑1 (MQ=255) tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAAt < 1:685302/150‑1 (MQ=255) gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATaa < 2:14959/151‑1 (MQ=255) gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATa < 2:2517/150‑1 (MQ=255) tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGt < 1:24741/150‑1 (MQ=255) aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGa < 1:900648/151‑1 (MQ=255) aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAA‑TTTTTTCA‑ACAAAAAAAAAAAACGGAGTTGTCATGATGAGGGGAAGGAAA‑AAACAAC‑AAGCAGTGGAAAAATTggaa > 1:629543/1‑147 (MQ=255) tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGAtt < 2:958237/150‑1 (MQ=255) aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGt < 1:364121/151‑1 (MQ=255) aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑ATTTTTTAAACAAAAACAAAAAAAGGAGTTGGCATGATGAGCGAAAGAAAT‑AAAAAAA‑AAACAGGGAATAAATTGAAAgaga > 2:856181/1‑147 (MQ=255) aaTTACATATTCACGGTGGC‑AAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGt < 1:276463/150‑1 (MQ=255) aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGC‑G‑AA‑TTTTTTCACACAAAAACAAAAACAGGGGCTGCTATGTTGAGTGGAAGTAATAAAGCAA‑‑AAGAAGTGAATaaagttcccgagatt > 2:716658/1‑135 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGTCGCTAAc < 1:735131/150‑1 (MQ=255) tCACGGTGGCAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGCGGCGGG‑G‑AACTTTTTTAAAACAAA‑ACAAAAAAAGGAGTTTGCATGATGAGCGAAAGTAAT‑AAACAAC‑AGCAAGAGTAAAAATGTACAGAGATTgaggctcaactt > 2:520790/1‑140 (MQ=255) tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTTCCGACAAATACAAAAACTGGAGTTGTCATGATGAGCGAAAATAAT‑AAACAAA‑AAGCAGGGAATAAATTGACAGAGAATGTCGCTAAAttt > 1:607308/1‑151 (MQ=255) tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt < 1:239981/150‑1 (MQ=255) ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg < 2:331576/151‑1 (MQ=255) gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTTCCGAAAAAAAAAAAAAATGGAGTTGCCATGATGAGCGAAAGGAAA‑AAACAAA‑AAGCAGGGAAAAAATTGACAGAGAGTTTCGCTAACTTTTCCGc > 1:467919/1‑151 (MQ=255) tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTTCCGACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGAAAGGAAT‑AAGCAAA‑AAGCAGTGGATAAGTTGACAGAGAATGTCGCTAACTTTACCGcc > 1:599826/1‑151 (MQ=255) aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATg < 1:286953/150‑1 (MQ=255) aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAtt < 1:48562/151‑1 (MQ=255) aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑G‑AA‑ATTTTTCAAACAAAAACAAAAAATGGAGTTGTCATGATGAGCGAAAGTAAT‑AAAAAAC‑AGGAAGAGAAAAAATTGACAGAGATTGTCGCTAACTTTACCGCCATGAttt > 2:677662/1‑150 (MQ=255) ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑ATTTTTCAAACAAAAAAAAAAAATTGAGTTGGCCTGATGGGCGGAAGGAAT‑AAGCAAA‑AGGCAGTGAATAAGTTGAAAGAGATTGTTGCTAAATTTAACCCCCAGAttttt > 2:893007/1‑147 (MQ=255) cacaTTTTTAGTGGTATTTTGTGGTGGT‑G‑AA‑TTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATg < 1:774086/151‑1 (MQ=255) cacaTTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTTCAGACAAAAACAAAAAATGGGGGTGGCATGATGAGCGAAAGGAAT‑AAACAAA‑AGGCAGTGGAAAAATTGACAGAGAATGTCGCTAACTTTTCCGCCCTGATTTTTACCCCaa > 1:398543/1‑149 (MQ=255) acaTTTTTAGTGGTAGTTTGTGGCGGG‑G‑AC‑TTTTTTCCGACAAAAACAAAAAATTGAGGTGCCATGATTAGGGAAAAGAAT‑AAACAAA‑AGGCAGGGAATAAAATGACAGAAATTTTCCCTAAACTTAACCCCATGGTTTTTACCCCAATg > 1:869705/1‑150 (MQ=255) tAGTGGTAGTTTGTGGCGGTGG‑AA‑TTTTTCC‑GACAAATAAAAAAAATGGAGTTTGCCTGATGAGGGGAAGTAAT‑AAACAAA‑AGGGAGAGAAAAAATTTAAAGAGATTTTTGCTAAATTTTCCCCCCCGATTTTTAACCCaa > 2:250886/1‑141 (MQ=255) gTGGTAGTTTGTGGCGGT‑G‑TA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTAAATAAGTTAACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGGCg < 2:672659/151‑1 (MQ=255) gTGGTAGTTTGTGGCGGC‑G‑AA‑TTTTTCCAGACAAATACAAAAACAGGAGGTGCCATGATTGGGGAAAGGAAC‑AAGAAAC‑AGGCAGGGAAACAGAGGACAGAGATTGTGGCGTACTTTTCCGCCGTGCGTTTTTCCCTCATGCCtggtg > 2:770726/1‑148 (MQ=255) ggTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACCAAAAAAAAAACTGGAGGTGGCCTGGTGAGGGAAAATAAT‑AAAGAACAAGGCGGGAAAAAAATTGA‑AGAGAATGTCGGCAACTTTACCCCCCTGATTTCTAACCGAAAGCCCGATGAAgtg > 2:538487/1‑151 (MQ=255) tttGTGGTTGT‑A‑AA‑TTTTTCTAGACAAATTCAAATACTGGATTTGCCATTATGGGCGAAATTAAT‑AGCCAAC‑GGGGTGTTAATAAGTTTACAGAGTTGGACACTCGCTTTCCCGCCATGATTTCTACCCGAATGCCtgatga < 2:184690/142‑1 (MQ=255) tgGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa > 1:74222/1‑150 (MQ=255) ggCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc > 1:694017/1‑151 (MQ=255) ggT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa > 2:381627/1‑150 (MQ=255) gT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa > 1:920754/1‑150 (MQ=255) aa‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc < 1:303054/150‑1 (MQ=255) ccAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa < 1:849870/150‑1 (MQ=255) | TTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAAC‑AGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA > NC_000913/3206293‑3206584 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |