Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,508,6620TA42.5% 57.8 / 13.6 40E384D (GAA→GAT) yhfWphosphopentomutase‑related metalloenzyme superfamily protein
Reads supporting (aligned to +/- strand):  ref base T (3/20);  new base A (17/0);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 2.57e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCAC  >  NC_000913/3508521‑3508811
                                                                                                                                             |                                                                                                                                                     
ttttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATcaca                                                                                                                                                  >  1:351927/1‑147 (MQ=255)
 tttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACg                                                                                                                                              <  1:954756/150‑1 (MQ=255)
      aGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACGGTa                                                                                                                                           >  1:631755/1‑148 (MQ=255)
          ggAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGTGCGCGGGGGAAAAATTACAAACAGGAGccccc                                                                                                                                     >  2:383484/1‑150 (MQ=255)
           gagagaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCcac                                                                                                                                   <  2:509056/151‑1 (MQ=255)
                 tCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGCGCGGGGGAAAAATCACACACCGCAGACCCcac                                                                                                                                   >  1:128164/1‑145 (MQ=255)
                  cAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGaa                                                                                                                            <  1:123990/151‑1 (MQ=255)
                  cAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGaa                                                                                                                            <  2:272444/151‑1 (MQ=255)
                          aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATAAAAAACGGGAGCCCCCCCAACAGAAAgagggg                                                                                                                     >  1:36045/1‑145 (MQ=255)
                            aTGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTgc                                                                                                                  <  1:676034/151‑1 (MQ=255)
                               cTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAAATACACACGGTAGCCCCCCCATCAGAAAGCgcggg                                                                                                                    >  2:661884/1‑145 (MQ=255)
                                 cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGAAAAATTTACACCCCGTAGCCCCCCCATCAGAAAAAGGGGGGCGcac                                                                                                              >  1:361665/1‑150 (MQ=255)
                                    gggCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGGGCGGGAAAAAAAAAACACAGGAGACCCCCCATCAGAAAGCGGGGGgcg                                                                                                                 >  2:520937/1‑144 (MQ=255)
                                    gggCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCg                                                                                                          <  2:114659/151‑1 (MQ=255)
                                     ggCGAGGGAGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGGCCGCGGAAAAAATTACAAACGGGAGCCCCCCCCTCAGAAAGGGGGGGGCCGACAcc                                                                                                           >  2:595482/1‑149 (MQ=255)
                                                   ccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatc                                                                                           <  1:301206/151‑1 (MQ=255)
                                                       cGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTAAACACGGTAGCCCCCCCCTCAGAAAGCGGGGGGCGCACACCGAGCTGCGTAGCGATCATcc                                                                                       >  1:44257/1‑151 (MQ=255)
                                                                     tAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGGGCGCGGGAAAAATCACACACGGGAGCCCCCCCATCAGAAAGCGGGGGGCGCACACCCAGCTGCGTAGCGATCATCCCCTGCTGATaaa                                                                           >  1:589175/1‑149 (MQ=255)
                                                                      aGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGAAAAAATTACACACGGGAGCCCCCACATCAGAAAGCGGGGTGCGCACCCCCAGCTGCGTAGCGATCATCCCTTGCTGATaaa                                                                           >  1:884673/1‑148 (MQ=255)
                                                                      aGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAcc                                                                         <  2:46049/150‑1 (MQ=255)
                                                                        ggTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAAACACACACCGGAGCCCCCCCAACAGAAAACGGGGTGCGCACACCCAGCTGCGGAGCGGTCATCCCTTGCTGGTAAAACAGc                                                                      >  1:282272/1‑151 (MQ=255)
                                                                               ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGca                                                               <  1:837244/151‑1 (MQ=255)
                                                                                cTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGGGCGGGAAAAATCACACACCGGAGCCCCCACAACAGAAAAGGTGGGGCGCACACCCAGCCGGGTAGCGAGCATCCCTTTCTGATAAACCACCACCGGcac                                                              >  1:445735/1‑151 (MQ=255)
                                                                                 tGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACGGGGGCCCCCACATCAGAAAACGGGGGGCGCACACCCAGCTGCGCAGCGGTCATCCCCTGCTGGTAAAACAACACTGGcccc                                                             >  1:283626/1‑151 (MQ=255)
                                                                                   cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAATTACAAACGGGTGCCCCCCCCTCCGAAAGGGGGGGGCGCACCCCCAGCCGCGGAGCGATCATCCCCTGCTTGTAAAacaacaacagccccac                                                           >  2:737774/1‑141 (MQ=255)
                                                                                    aaaCCGGAGGGAGGAAAGAAAAGAGCGACCATCTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACt                                                          <  2:355244/151‑1 (MQ=255)
                                                                                     aaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGGGCGGGAAAAATTACACACGGTAGCCCCCCCACCAGAAAACGGGGGGCGCACACCGAGCTGCGGAGCGGCCAACCCTTGCTGATAAACAAGCACCGGccccac                                                           >  2:77660/1‑149 (MQ=255)
                                                                                      aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTc                                                        <  1:167811/151‑1 (MQ=255)
                                                                                          gagggaggAAAGAAAAGAGCGGCCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAATCCAGCACTGGCACCGCTTcgc                                                      <  2:341420/149‑1 (MQ=255)
                                                                                            gggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAATTACACACCGGAACCCCCACACAAGAAAGCGGGGGGCGCACACCGAGCTGCGTAGCGGTCACCCCCTTCTGATAAAAAAGCCCAGGCCCCACATCGCgggg                                                  >  2:895977/1‑150 (MQ=255)
                                                                                                       aaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCtgt                                       <  2:636943/151‑1 (MQ=255)
                                                                                                       aaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCtgt                                       <  1:145632/151‑1 (MQ=255)
                                                                                                               aCCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATg                               <  2:657972/151‑1 (MQ=255)
                                                                                                                ccATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATgg                              <  1:397820/151‑1 (MQ=255)
                                                                                                                         ggcggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCg                      <  1:814678/150‑1 (MQ=255)
                                                                                                                          gcggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGtt                    <  1:750460/151‑1 (MQ=255)
                                                                                                                           cggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTg                   <  2:560339/151‑1 (MQ=255)
                                                                                                                            ggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGc                  >  1:262977/1‑151 (MQ=255)
                                                                                                                                       gAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGcc       >  1:569938/1‑151 (MQ=255)
                                                                                                                                            aTTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCAc  <  1:156286/151‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                     
TTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCAC  >  NC_000913/3508521‑3508811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑