| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 298,960 | 0 | A | C | 39.1% | 72.6 / 17.6 | 47 | Y659D (TAT→GAT) | paoC | PaoABC aldehyde oxidoreductase, Moco‑containing subunit |
| Reads supporting (aligned to +/- strand): ref base A (25/3); new base C (0/18); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.72e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTTATACACCGCGTTGGCGATAGCCGCGCTCACGCCGCACAGGCCCAGCTCACCGACACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACACAGC > NC_000913/298810‑299110 | cGTTATACACCGCGTTGGCGATAGCCGCGCTCACGCCGCACAGGCCCAGCTCACCGACACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATa < 2:233094/151‑1 (MQ=255) acCGCGTTGGCGATAGCCGCGCTCACGCCGCACAGGCCCAGCTCACCGACACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCa < 1:839017/151‑1 (MQ=255) cgcgTTGGCGATAGCCGCGCTCACGCCGCACAGGCCCAGCTCACCGACACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCat > 1:706170/1‑150 (MQ=255) cgcgTTGGCGATAGCCGCGCTCACGCCGCACAGGCCCAGCTCACCGACACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCat > 1:519038/1‑150 (MQ=255) gTTGGCTATAGCCGCGCTCACGCCGGACAGGCACGGCTCACCGTCACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGTAAAATCGCCGCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATGCCCCg < 2:128163/143‑1 (MQ=255) tGGCAATGGCCGCGCTCACGCCGCACAGGCCCAGCTCCCCAACCCCTTTGCCCTTCAACGGGGAGGATAGGTGGTCGTTGTCACCCGGGAAACTCCCCCCCTGTTTTGGGTTCTCCGCGGGAACCGCCA‑CCTCCTCCGCCGCCATAGCGt < 2:913697/150‑1 (MQ=255) cgcgCTCACGCCGCACAGGCCCAGCTCACCGACCCCTTTGGCCTTCATCGGGGAGGATATGGGGTGGGTATCATCCAGAAAAATCCCCTCCTGTTTTGGGATATCCGCATGACCCGGCC‑CCTCATCCCCCGCCATATCGTGATTAACGAAg < 1:927557/151‑1 (MQ=255) gcccgcgccgaccgggCCCAGCCCCCCGACCCCTTTGGCCTTCAGCGGGGAGGCTATGGGGTCGGATTCATCCAGGAAAACCCCCTCCGGTTTGGGGATATCCGAATAACCCGGCA‑CCTCACACCCCGCCATATCGTGATTAACGAAGTa < 2:88622/136‑1 (MQ=255) cgccgcACAGGCCCAGCTCACCGACACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCa > 1:240804/1‑150 (MQ=255) ggCCCAGCTCACCGACACCTTTGGCTTTCATGGGGGAGGATAGGGGGTCGTTATCACCCAGGAAAACCCCCCCCTTTTTTGGGATATCCGCATGAACCGGCA‑CCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCa < 1:8693/149‑1 (MQ=255) gCCCAGCTCACCGACACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATcc > 1:215870/1‑151 (MQ=255) cccAGCTCACCGACCCCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGAAAAACCCCCTCCTTTTTGGGGATATCCGCATGACCCGGCC‑CCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCa < 2:292749/151‑1 (MQ=255) tCACCGACACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGcc > 1:824762/1‑150 (MQ=255) aCCGACACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGc > 1:6915/1‑151 (MQ=255) acacCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGctcct > 1:32233/1‑151 (MQ=255) ttgccctccaggggggggGATAGGGGGGGGTTATCACCCAGAAAACCCCCCCCCTTTTTGGGGATCCCCGAATGACCCGCCC‑CCTCACCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCtcctcc < 2:656577/135‑1 (MQ=255) tgggCTTTCAGGGGGGAGGATAGGGGGTCGTTATCACCCAGAAAAACCCCCTCCTTTTTGGGGATACCCGCATGACCCGGCC‑CCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATc < 1:504220/148‑1 (MQ=255) gCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTg > 1:148453/1‑151 (MQ=255) ttCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCcg > 2:925632/1‑151 (MQ=255) ggggggAGTATAGGGGGTCGTTATCACCCAGAAAATCCCCCTCCTTTTTGGGAATCTCCGCAAGACCCGCCC‑CCTCCTCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCgcc < 2:791460/149‑1 (MQ=255) cGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCgccc > 2:351708/1‑151 (MQ=255) cGGGGAGGATATGGGGTCGGAATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCgccc > 2:395202/1‑151 (MQ=255) tttgggtccGGTATCACCCAGGAAAACCGCCTCTTTTTTGGGGAAATCCGCATGACCGGGCA‑CCTCTTACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCgcccatgcccat < 1:286438/142‑1 (MQ=255) gggggTCGGTATCACCCAGGAAAACCCCCTCCTTTTTTGGGATACCCGCATGACCCGCCC‑CCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGt < 1:35776/150‑1 (MQ=255) ggggTCGTTATCACCAAGAAAAACCCCCCCCTGTTTGGGAATTCCCCAATAACCCGCCC‑CCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCgcccatgcccat < 1:820866/147‑1 (MQ=255) ggggTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTc > 1:171050/1‑151 (MQ=255) cGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTgc > 2:362493/1‑151 (MQ=255) tATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCACGCCCATAGTCATTGCGcc > 2:436057/1‑151 (MQ=255) atcCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATg > 2:921238/1‑151 (MQ=255) cccAGAAAAACCCCCCCCTGTTTGGGGATACCCGCATGACCCGCCC‑CCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGa < 1:797761/150‑1 (MQ=255) caaGGAAAACCCCCTCCTGTTGGGGTATACCCGCATGAACCGGCC‑CCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATg < 2:431443/147‑1 (MQ=255) cAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGAcc > 1:271816/1‑151 (MQ=255) cAGAAAAATCCCCTCCTTTTTGGGAATATCCGCATGACCCGGCC‑CCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGAcc < 1:814858/151‑1 (MQ=255) gaaaacccccctcttGTTTGGGGTTACCCGTA‑CAACCGGCACCCTCAACCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCt < 2:610048/141‑1 (MQ=255) aaaaaCCCCCTCCTGTTTGGGGAAACCCGCAGAACCCGCCC‑CCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTg < 2:83528/149‑1 (MQ=255) ccccccctTTTTTGGGATACCCGAATAACCCGCCC‑CCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTg < 1:231735/143‑1 (MQ=255) cACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTgcgc > 1:377470/1‑151 (MQ=255) gggATACCCGCATGACCCGGCC‑CCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGAt < 1:535709/151‑1 (MQ=255) ccGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGAt > 1:466398/1‑151 (MQ=255) cGCATAACCCGGCC‑CCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATg < 1:759024/151‑1 (MQ=255) gAACCGGCA‑CCTCATACCCCCACATATCGTGATTACCGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCCTAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTcc > 2:776895/1‑151 (MQ=255) cGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTgc > 2:681255/1‑150 (MQ=255) ggCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTgc > 2:475605/1‑149 (MQ=255) ggCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTgc > 1:262319/1‑149 (MQ=255) cgaccccTCACCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCt < 2:906090/143‑1 (MQ=255) cA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTgcagc > 1:592085/1‑150 (MQ=255) cATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGcacacac > 1:145436/1‑151 (MQ=255) tACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGcacacac > 1:168413/1‑149 (MQ=255) aCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACACAGc > 2:199585/1‑151 (MQ=255) | CGTTATACACCGCGTTGGCGATAGCCGCGCTCACGCCGCACAGGCCCAGCTCACCGACACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCA‑CCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACACAGC > NC_000913/298810‑299110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |