Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913907,5590TC40.4% 87.3 / 11.9 57I242V (ATT→GTT) ybjTputative NAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (22/12);  new base C (0/23);  total (22/35)
Fisher's exact test for biased strand distribution p-value = 1.73e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCT  >  NC_000913/907412‑907698
                                                                                                                                                    |                                                                                                                                              
ccGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCa                                                                                                                                            <  1:439439/151‑1 (MQ=255)
   cgTCATCGAAAGCGATCAGCCGTTGTGGGATGATTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCg                                                                                                                                        <  1:487085/151‑1 (MQ=255)
      cATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGt                                                                                                                                     <  2:319842/151‑1 (MQ=255)
       aTCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTg                                                                                                                                    <  2:689850/151‑1 (MQ=255)
         cGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTgg                                                                                                                                   <  2:686662/150‑1 (MQ=255)
                 aTCAGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAggggg                                                                                                                           >  2:746666/1‑150 (MQ=255)
                    aGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCACTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGggggatgg                                                                                                                       >  1:506991/1‑151 (MQ=255)
                       cGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGggggatgggga                                                                                                                    <  2:680773/151‑1 (MQ=255)
                       cGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGggggatgggg                                                                                                                     >  2:547899/1‑150 (MQ=255)
                          tgtgGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATc                                                                                                                  <  1:364250/150‑1 (MQ=255)
                             gggATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGAGCGGGTTTCGGCCCCTGAATCGACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAAc                                                                                                               <  1:92845/150‑1 (MQ=255)
                               gATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAAcc                                                                                                              >  1:291856/1‑149 (MQ=255)
                               gATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAg                                                                                                            <  1:674516/151‑1 (MQ=255)
                                      gCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCt                                                                                                    >  2:649159/1‑151 (MQ=255)
                                        aCGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCtt                                                                                                   >  2:790352/1‑150 (MQ=255)
                                          gTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTAcc                                                                                                <  1:397754/151‑1 (MQ=255)
                                              cgcgGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTc                                                                                            >  1:638383/1‑151 (MQ=255)
                                              cgcgGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTc                                                                                            >  1:145528/1‑151 (MQ=255)
                                              cgcgGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTc                                                                                            <  2:650247/151‑1 (MQ=255)
                                                     tcatcCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGcc                                                                                      >  1:876896/1‑150 (MQ=255)
                                                                   ttggtttttcgcccctTGAATCACCGCCCGGGCGGTGGGGGGGGGTAGGGAGGTAATCAAATTGAAAACCCCCCCCAAAACCCAGGGGGTGGGGGGGGGGAGGGGGATCACCCGGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCaaa                                                                        <  1:315144/138‑1 (MQ=255)
                                                                       tgtTTCCGCCCCTGAATCACCGCCCTGGGGGTGGGGGGGGGTACGGAAGTAATCACATTGAGAAACCACACCAAAACCCAGGGGGGGGGGGGGGGGAGGGGGATCAACCAGCGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGt                                                                    <  1:831162/150‑1 (MQ=255)
                                                                            cAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTg                                                               >  1:375002/1‑150 (MQ=255)
                                                                               ccccTGAACCACCCCCCGGGGGGGGGGGGGGGGTAGGAAGGTACGCACATGGAGAACCCACCCCAAACCCCAGCGGGGGGGGAGGGGGAGGGTGATCAGCCGCCGGCCCTTCCCACCCCCCGCCATAAAATGTTCAAACTGTTGCTGAt                                                             <  2:279881/149‑1 (MQ=255)
                                                                                 ccgaaaccAACGCCCTGGGGGTGGTGGGGGGTGCGGAAGTAATCCCAGTGAGAACCTACCCCGAACCCCAGCGGGTGGGGGGGGGGATGGGAATCAACCGGCGGCGCTTACCCCACACCGCCATAAAATGTTCAAACTGTTGCTGATAACt                                                         <  2:236625/144‑1 (MQ=255)
                                                                                 ccTAAATCACCGCCGTGGGGGTGGTGGGGGGTAGGGAAGTAATCACATTGAAAACCCACCCCAAAACCCGGGGGGTGGGGGGGGGGAGGGGGATAAACCAGCGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACt                                                         <  1:634474/151‑1 (MQ=255)
                                                                                     aaacAACGCCCGGGGGGTGGGGGGGGGTACGGAGGTAATCACATTGAGAAACCACCCCAAAACCCGGGGGGGGGGGGGGGGGATGGGGATCACCCGGGGGCTCTTCCCGCTCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGc                                                     <  2:944170/148‑1 (MQ=255)
                                                                                     aaTCACCCCCCGGGGGGTGGGGGGCGGTACGGAATTAATCACATTGAAAACCCCCCCCAAAACCCGGGGGGTGGGGGGGGGGAGGGGGATCAACCAGGGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAg                                                      <  1:492116/150‑1 (MQ=255)
                                                                                       tCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCAc                                                   >  1:151985/1‑151 (MQ=255)
                                                                                         accccccTGGGGGGGGGGGGGGGTACGGAAGTAATCACATTGAAAACCCACCCCAAAACCCAGGGGGGGGGGGGGGGGAGGGGAACCACCCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCa                                                    <  1:296112/144‑1 (MQ=255)
                                                                                         aaCGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACct                                                 >  1:783340/1‑151 (MQ=255)
                                                                                           cGCCGTGGCGGTGGGGGGAGGGACGGAGCTAATCATTGTGAAAATCCCCCCGGAACCCGCGCGGGTGGGGAGGGGGATGGGTAGCACCAACGGACGCTTCCCCCACCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGc                                                     <  2:708785/145‑1 (MQ=255)
                                                                                           cGCCCGGGGGGGGGTGGGGGGCACGAAAGTAATCACATTGACAACCCACACCAAATCCCCGGGGGTGGGGAGGGGGAGGGGGAACAGCCAGCGCCGCCAACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACctc                                                <  2:478420/150‑1 (MQ=255)
                                                                                              ccTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTgg                                             >  2:291003/1‑150 (MQ=255)
                                                                                                 gggggtggtGGGCGGTACGAAATTAACAAAATGGAGAACCCCCCCCGAAACCCAGCGGGGGGGGGGGGGGAGGGGGACCACCCAGCGCCGCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcg                                         <  2:786838/148‑1 (MQ=255)
                                                                                                   cGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcggc                                       >  1:442112/1‑151 (MQ=255)
                                                                                                   cGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGGTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcgg                                        >  2:192087/1‑150 (MQ=255)
                                                                                                    ggtggtGGGCGGTAGGAAAGAAATCACATTGAGAACCCACCCCAAACCCCGGGGGGGGGGGGGGGGGAGGGGAATCAACCAGCGGCGTTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcggcg                                      <  1:182613/151‑1 (MQ=255)
                                                                                                     gggggggggggTACGGAAGTAATCAAATTGAAAAACCCCCCCAAAACCCGGGGGGGGGGGGGGGGGATGGGAATCAACCGGCGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcggcgg                                     <  1:143846/146‑1 (MQ=255)
                                                                                                       ggtggGCGGTACGGAAGTAATCACATGGAAAAACCCCCCCAAACCCCGGGGGGGGGGGGGGGGGATGGGGATCAACCAGCGGCGTTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcggcg                                      <  1:104113/148‑1 (MQ=255)
                                                                                                       ggggggggtaaCGGAAGTAATGACAGAGAAAACCCACCCAGAATCCAGGCGGTTGGGGGGGGGGAGGGGGACGAGCCAGCGCCGCTACCCCCCCGCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTc                                           <  2:739819/133‑1 (MQ=255)
                                                                                                           ggCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCg                                >  2:659195/1‑150 (MQ=255)
                                                                                                              ggTAGGGAAGTAATCACATGGAGAACCCACACCAAAACCCGGGGGGTGGGGGGGGGGAGGGGGATCAACCGGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAg                             <  1:327400/150‑1 (MQ=255)
                                                                                                              ggTACGGAAGAAACCATATGGAAAACCCCCCCCAAACCCCGGGGGGGGGGGGGGGGGAGGGGAATCAACCGGGGGCTCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAg                             <  1:305878/150‑1 (MQ=255)
                                                                                                                  cGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCg                        >  1:358737/1‑151 (MQ=255)
                                                                                                                   ggAACTAATCATATTAAAAACCCACCCCAAACCCCGGGGGGTGGGGGGGGGGAGGGGAACCAACCAGGGGCCTTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCg                        <  1:695511/150‑1 (MQ=255)
                                                                                                                    gAATTATTCACATTGAGAACCCACCCGAACCCCAAGGGGGGGGGGAGGGGTATGGGAATAACCCGGGGCCGCTTCCCACTCACGGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGag                           <  2:111970/146‑2 (MQ=255)
                                                                                                                    gAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCgg                       >  2:663745/1‑150 (MQ=255)
                                                                                                                     aattAATCAAATGGAGAACCCACACCAAAACCCGGGGGGGGGGGGGGGGGAGGGGAATCACCCAGCGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCgg                       <  1:121655/146‑1 (MQ=255)
                                                                                                                         aaTCACATTAAAAACCCACCCCGAAACCCGGGGGGGGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGtt                   <  1:758199/149‑1 (MQ=255)
                                                                                                                         aaTCACATGAAAAACCCCCCCGAAAACCCGGGGGGGGGGGGGGGGGGTGGGAATCACCCACGGGCCCTTCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCg                 <  2:373357/151‑1 (MQ=255)
                                                                                                                             acaTTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCCTCGAAGATGCGGTGTTCGCTgg             >  2:128356/1‑151 (MQ=255)
                                                                                                                              caTTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGc            >  2:16538/1‑151 (MQ=255)
                                                                                                                               aTTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGcc           >  1:299113/1‑151 (MQ=255)
                                                                                                                                   aaacaccaccCCGAACCCCGGGGGGTGGGGGGGGGGTTGGGGATCACCGAGCGGCGCTCCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGgat       <  2:187172/147‑1 (MQ=255)
                                                                                                                                   aaaacccACACCAAAACCCAGGGGGGGGGGAGGGGGAGGGGGATCAACCGGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCg          <  1:814447/143‑1 (MQ=255)
                                                                                                                                        ccACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCt  >  1:140953/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCT  >  NC_000913/907412‑907698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: