Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 907,559 | 0 | T | C | 40.4% | 87.3 / 11.9 | 57 | I242V (ATT→GTT) | ybjT | putative NAD‑dependent oxidoreductase |
Reads supporting (aligned to +/- strand): ref base T (22/12); new base C (0/23); total (22/35) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.73e-07 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCT > NC_000913/907412‑907698 | ccGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑a < 1:439439/151‑1 (MQ=255) cgTCATCGAAAGCGATCAGCCGTTGTGGGATGATTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCg < 1:487085/151‑1 (MQ=255) cATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGt < 2:319842/151‑1 (MQ=255) aTCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTg < 2:689850/151‑1 (MQ=255) cGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTgg < 2:686662/150‑1 (MQ=255) aTCAGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAggggg > 2:746666/1‑150 (MQ=255) aGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCACTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGggggatgg > 1:506991/1‑151 (MQ=255) cGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGggggatgggga < 2:680773/151‑1 (MQ=255) cGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGggggatgggg > 2:547899/1‑150 (MQ=255) tgtgGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATc < 1:364250/150‑1 (MQ=255) gggATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGAGCGGGTTTCGGCCCCTGAATCGACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAAc < 1:92845/150‑1 (MQ=255) gATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAAcc > 1:291856/1‑149 (MQ=255) gATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAg < 1:674516/151‑1 (MQ=255) gCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCt > 2:649159/1‑151 (MQ=255) aCGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCtt > 2:790352/1‑150 (MQ=255) gTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTAcc < 1:397754/151‑1 (MQ=255) cgcgGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTc > 1:638383/1‑151 (MQ=255) cgcgGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTc > 1:145528/1‑151 (MQ=255) cgcgGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTc < 2:650247/151‑1 (MQ=255) tcatcCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGcc > 1:876896/1‑150 (MQ=255) ttggtttttcgcccctTGAATCACCGCCCGGGCGGTGGGGGGGGGTAGGGAGGTAATCAAATTGAAAACCCCCCCCA‑AAACCC‑A‑GGGGGTGGGGGGGGGGAGGGGGATCACCCGGCGG‑CGCTTCCCGCTCACCGCCATAAAATGTTCaaa < 1:315144/138‑1 (MQ=255) tgtTTCCGCCCCTGAATCACCGCCCTGGGGGTGGGGGGGGGTACGGAAGTAATCACATTGAGAAACCACACCA‑AAACCC‑A‑GGGGGGGGGGGGGGGGAGGGGGATCAACCAGCGG‑CGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGt < 1:831162/150‑1 (MQ=255) cAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTg > 1:375002/1‑150 (MQ=255) ccccTGAACCACCCCCCGGGGGGGGGGGGGGGGTAGGAAGGTACGCACATGGAGAACCCACCCCA‑AACCCC‑A‑GCGGGGGGGGAGGGGGAGGGTGATCAGCCGCCGG‑CCCTTCCCACCCCCCGCCATAAAATGTTCAAACTGTTGCTGAt < 2:279881/149‑1 (MQ=255) ccgaaaccAACGCCCTGGGGGTGGTGGGGGGTGCGGAAGTAATCCCAGTGAGAACCTACCCCG‑AACCCC‑A‑GCGGGTGGGGGGGGGGATGGGAATCAACCGGCGG‑CGCTTACCCCACACCGCCATAAAATGTTCAAACTGTTGCTGATAACt < 2:236625/144‑1 (MQ=255) ccTAAATCACCGCCGTGGGGGTGGTGGGGGGTAGGGAAGTAATCACATTGAAAACCCACCCCA‑AAACCC‑G‑GGGGGTGGGGGGGGGGAGGGGGATAAACCAGCGG‑CGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACt < 1:634474/151‑1 (MQ=255) aaacAACGCCCGGGGGGTGGGGGGGGGTACGGAGGTAATCACATTGAGAAACCACCCCA‑AAACCC‑G‑GGGGGGGGGGGGGGGGATGGGGATCACCCGGGGG‑CTCTTCCCGCTCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGc < 2:944170/148‑1 (MQ=255) aaTCACCCCCCGGGGGGTGGGGGGCGGTACGGAATTAATCACATTGAAAACCCCCCCCA‑AAACCC‑G‑GGGGGTGGGGGGGGGGAGGGGGATCAACCAGGGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAg < 1:492116/150‑1 (MQ=255) tCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCAc > 1:151985/1‑151 (MQ=255) accccccTGGGGGGGGGGGGGGGTACGGAAGTAATCACATTGAAAACCCACCCCA‑AAACCC‑A‑GGGGGGGGGGGGGGGGAGGGGAACCACCCAGCGG‑CGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCa < 1:296112/144‑1 (MQ=255) aaCGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACct > 1:783340/1‑151 (MQ=255) cGCCGTGGCGGTGGGGGGAGGGACGGAGCTAATCATTGTGAAAATCCCCCCGG‑AACCCG‑C‑GCGGGTGGGGAGGGGGATGGGTAGC‑ACCAACGGACGCTTCCCCCACCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGc < 2:708785/145‑1 (MQ=255) cGCCCGGGGGGGGGTGGGGGGCACGAAAGTAATCACATTGACAACCCACACCA‑AATCCC‑C‑GGGGGTGGGGAGGGGGAGGGGGAACAGCCAGCGC‑CGCCAACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACctc < 2:478420/150‑1 (MQ=255) ccTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTgg > 2:291003/1‑150 (MQ=255) gggggtggtGGGCGGTACGAAATTAACAAAATGGAGAACCCCCCCCG‑AAACCC‑A‑GCGGGGGGGGGGGGGGAGGGGGACCACCCAGCGC‑CGCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcg < 2:786838/148‑1 (MQ=255) cGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcggc > 1:442112/1‑151 (MQ=255) cGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGGTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcgg > 2:192087/1‑150 (MQ=255) ggtggtGGGCGGTAGGAAAGAAATCACATTGAGAACCCACCCCA‑AACCCC‑G‑GGGGGGGGGGGGGGGGAGGGGAATCAACCAGCGG‑CGTTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcggcg < 1:182613/151‑1 (MQ=255) gggggggggggTACGGAAGTAATCAAATTGAAAAACCCCCCCA‑AAACCC‑G‑GGGGGGGGGGGGGGGGATGGGAATCAACCGGCGG‑CGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcggcgg < 1:143846/146‑1 (MQ=255) ggtggGCGGTACGGAAGTAATCACATGGAAAAACCCCCCCA‑AACCCC‑G‑GGGGGGGGGGGGGGGGATGGGGATCAACCAGCGG‑CGTTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcggcg < 1:104113/148‑1 (MQ=255) ggggggggtaaCGGAAGTAATGACAGAGAAAACCCAC‑CCA‑GAATCC‑AGGCGGTTGGGGGGGGGGAGGGGGACGAGCCAGCGC‑CGCTACCCCCCCGCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTc < 2:739819/133‑1 (MQ=255) ggCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCg > 2:659195/1‑150 (MQ=255) ggTAGGGAAGTAATCACATGGAGAACCCACACCA‑AAACCC‑G‑GGGGGTGGGGGGGGGGAGGGGGATCAACCGGCGG‑CGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAg < 1:327400/150‑1 (MQ=255) ggTACGGAAGAAACCATATGGAAAACCCCCCCCA‑AACCCC‑G‑GGGGGGGGGGGGGGGGAGGGGAATCAACCGGGGG‑CTCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAg < 1:305878/150‑1 (MQ=255) cGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCg > 1:358737/1‑151 (MQ=255) ggAACTAATCATATTAAAAACCCACCCCA‑AACCCC‑G‑GGGGGTGGGGGGGGGGAGGGGAACCAACCAGGGG‑CCTTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCg < 1:695511/150‑1 (MQ=255) gAATTATTCACATTGAGAACCCAC‑CCG‑AACCCCAA‑GGGGGGGGGGAGGGGTATGGGAATAACCCGGGGC‑CGCTTCCCACTCACGGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGag < 2:111970/146‑2 (MQ=255) gAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCgg > 2:663745/1‑150 (MQ=255) aattAATCAAATGGAGAACCCACACCA‑AAACCC‑G‑GGGGGGGGGGGGGGGGAGGGGAATCACCCAGCGG‑CGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCgg < 1:121655/146‑1 (MQ=255) aaTCACATTAAAAACCCACCCCG‑AAACCC‑G‑GGGGGGGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGtt < 1:758199/149‑1 (MQ=255) aaTCACA‑TGAAAAACCCCCCCGAAAACCC‑G‑GGGGGGGGGGGGGGGGGTGGGAATCACCCACGGG‑CCCTTCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCg < 2:373357/151‑1 (MQ=255) acaTTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCCTCGAAGATGCGGTGTTCGCTgg > 2:128356/1‑151 (MQ=255) caTTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGc > 2:16538/1‑151 (MQ=255) aTTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGcc > 1:299113/1‑151 (MQ=255) aaacaccaccCCG‑AACCCC‑G‑GGGGGTGGGGGGGGGGTTGGGGATCACCGAGCGG‑CGCTCCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGgat < 2:187172/147‑1 (MQ=255) aaaacccACACCA‑AAACCC‑A‑GGGGGGGGGGAGGGGGAGGGGGATCAACCGGCGG‑CGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCg < 1:814447/143‑1 (MQ=255) ccACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCt > 1:140953/1‑151 (MQ=255) | CCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCG‑AAATCC‑A‑GCGGGTGGGGAGGGGGATGGGGATCAACCAGCGG‑CGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCT > NC_000913/907412‑907698 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |