Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 2,765,456 (TATGGCAC)6→5 intergenic (‑303/+50) yfjL ← / ← yfjM CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein/CP4‑57 prophage; uncharacterized protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2765412 2765419 8 66 [0] [0] 64 yfjL/yfjM CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein/CP4‑57 prophage; uncharacterized protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2765420 =0 (0.000)47 (1.050) 41/210 0.2 100% intergenic (‑267/+93) yfjL/yfjM CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein/CP4‑57 prophage; uncharacterized protein
?NC_000913 = 2765411 0 (0.000)intergenic (‑258/+102) yfjL/yfjM CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein/CP4‑57 prophage; uncharacterized protein

CAAGCTTTTCAGGATTCCACAAATATTGTAACCCTGACGTCTAATTTGGTTATATAACATACTGCCCCAACTAACGTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAACCGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCAACATC  >  NC_000913/2765271‑2765567
                                                                                                                                                     |                                                                                                                                                   
gcaggtatcaagcttttcaggattccacaaatattgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgc                                                                                                                                                    >  2:2144‑M1/150‑151 (MQ=255)
     tatcaagcttttcaggattccacaaatattgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactat                                                                                                                                               <  1:663226‑M1/7‑1 (MQ=255)
      atcaagcttttcaggattccacaaatattgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactatg                                                                                                                                              <  1:1650‑M1/8‑1 (MQ=255)
      atcaagcttttcaggattccacaaatattgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactatg                                                                                                                                              <  1:807725‑M1/8‑1 (MQ=255)
           gcttttcaggattccacaaatattgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactatggcact                                                                                                                                         <  1:172972‑M1/13‑1 (MQ=255)
           gcttttcaggattccacaaatattgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactatggcact                                                                                                                                         <  1:737951‑M1/13‑1 (MQ=255)
            cttttcaggattccacaaatattgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactatggcact                                                                                                                                         <  2:505229‑M1/13‑1 (MQ=255)
                 caggattccacaaatattgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactatggcactatggc                                                                                                                                    >  2:664341‑M1/133‑150 (MQ=255)
                   ggattccacaaatattgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactatggcactatggcact                                                                                                                                 <  2:500633‑M1/21‑1 (MQ=255)
                       tccacaaatattgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactatggcactatggcactatg                                                                                                                              >  2:268325‑M1/127‑150 (MQ=255)
                        ccacaaatattgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactatggcactatggcactatggc                                                                                                                            >  1:765436‑M1/126‑151 (MQ=255)
                           caaatattgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactatggcactatggcactatggcact                                                                                                                         <  2:669983‑M1/29‑1 (MQ=255)
                           caaatattgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactatggcactatggcactatggcact                                                                                                                         <  2:45357‑M1/29‑1 (MQ=255)
                             aatattgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactatggcactatggcactatggcactat                                                                                                                       >  2:970450‑M1/121‑151 (MQ=255)
                                attgtaaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactatggcactatggcactatggcactatggc                                                                                                                    >  1:518299‑M1/118‑151 (MQ=255)
                                     aaccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccgcactatggcactatggcactatggcactatggcactat                                                                                                               <  1:204368‑M1/39‑1 (MQ=255)
                                       ccctgacgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGcactatggcactatggcactatggcactatggcactatgg                                                                                                             >  2:589111‑M1/111‑151 (MQ=255)
                                             cgtctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGc                                                                                                       >  1:777353‑M1/105‑151 (MQ=255)
                                               tctaatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGcc                                                                                                      <  2:609884‑M1/48‑1 (MQ=255)
                                                  aatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCa                                                                                                     >  2:890627‑M1/100‑148 (MQ=255)
                                                  aatttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGc                                                                                                   >  2:602329‑M1/100‑150 (MQ=255)
                                                   atttggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCg                                                                                                  <  2:25792‑M1/52‑1 (MQ=255)
                                                       ggttatataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGt                                                                                             >  1:245334‑M1/95‑151 (MQ=255)
                                                           atataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAAt                                                                                          >  2:639540‑M1/91‑150 (MQ=255)
                                                           atataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAAt                                                                                          >  2:384660‑M1/91‑150 (MQ=255)
                                                             ataacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATcac                                                                                       <  2:829796‑M1/63‑1 (MQ=255)
                                                               aacatactgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATcaca                                                                                      >  1:932461‑M1/87‑150 (MQ=255)
                                                                     ctgccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGat                                                                                <  2:780404‑M1/70‑1 (MQ=255)
                                                                       gccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGatat                                                                              >  2:17837‑M1/79‑150 (MQ=255)
                                                                       gccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGAtatt                                                                             >  2:856704‑M1/79‑151 (MQ=255)
                                                                        ccccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGAtatta                                                                            >  1:974968‑M1/78‑151 (MQ=255)
                                                                          ccaactaacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGAtattat                                                                           <  1:796021‑M1/75‑1 (MQ=255)
                                                                               taacgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAac                                                                      <  2:355372‑M1/80‑1 (MQ=255)
                                                                                 acgtaaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAacac                                                                    <  1:785046‑M1/82‑1 (MQ=255)
                                                                                    taaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGtt                                                                <  1:963838‑M1/86‑1 (MQ=255)
                                                                                    taaggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGtt                                                                <  1:131574‑M1/86‑1 (MQ=255)
                                                                                      aggtaggcacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGtt                                                                >  1:757334‑M1/64‑149 (MQ=255)
                                                                                             cacagccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACGCAGTTGCACGCatt                                                       >  2:254236‑M1/57‑151 (MQ=255)
                                                                                                agccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCattatt                                                    <  2:79809‑M1/98‑1 (MQ=255)
                                                                                                agccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCattatt                                                    <  1:130787‑M1/98‑1 (MQ=255)
                                                                                                agccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCattatt                                                    <  1:468803‑M1/98‑1 (MQ=255)
                                                                                                  ccttaatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTc                                                  <  2:80429‑M1/100‑1 (MQ=255)
                                                                                                     taatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCt                                                >  2:796170‑M1/49‑150 (MQ=255)
                                                                                                      aatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCtgg                                              <  1:456817‑M1/104‑1 (MQ=255)
                                                                                                      aatcaaggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCtgg                                              >  2:156424‑M1/48‑151 (MQ=255)
                                                                                                            ggttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTa                                        >  2:562298‑M1/42‑151 (MQ=255)
                                                                                                              ttttggttttcaaatagttcagagctgagaccgccaaccGCACTATGGTACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCtggttgg                                          >  2:888722‑M1/40‑147 (MQ=255)
                                                                                                                    ttttcaaatagatcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCat                                 <  1:959418‑M1/117‑1 (MQ=255)
                                                                                                                       tcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATAtct                             <  2:742291‑M1/121‑1 (MQ=255)
                                                                                                                       tcaaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATAtc                              >  2:950185‑M1/31‑150 (MQ=255)
                                                                                                                        caaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATAtctc                            <  2:203865‑M1/122‑1 (MQ=255)
                                                                                                                        caaatagttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATAtctc                            <  2:853933‑M1/122‑1 (MQ=255)
                                                                                                                              gttcagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGct                      <  2:735976‑M1/128‑1 (MQ=255)
                                                                                                                                 cagagctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCtt                   <  2:528040‑M1/131‑1 (MQ=255)
                                                                                                                                      ctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTg                 >  1:534696‑M1/16‑148 (MQ=255)
                                                                                                                                      ctgagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGc              <  2:771308‑M1/136‑1 (MQ=255)
                                                                                                                                        gagaccgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCa             <  2:365650‑M1/137‑1 (MQ=255)
                                                                                                                                             cgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCa       >  2:756701‑M1/9‑151 (MQ=255)
                                                                                                                                             cgccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCa       >  1:505121‑M1/9‑151 (MQ=255)
                                                                                                                                               ccaaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCAAc     <  1:903388‑M1/145‑1 (MQ=255)
                                                                                                                                                caaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCAACa    >  2:856898‑M1/6‑151 (MQ=255)
                                                                                                                                                 aaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCAACAt   >  2:190143‑M1/5‑151 (MQ=255)
                                                                                                                                                 aaccGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCAACAt   <  1:474831‑M1/147‑1 (MQ=255)
                                                                                                                                                  accGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCAACATc  <  2:488827‑M1/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAAGCTTTTCAGGATTCCACAAATATTGTAACCCTGACGTCTAATTTGGTTATATAACATACTGCCCCAACTAACGTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAACCGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCAACATC  >  NC_000913/2765271‑2765567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.