Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,368,5580GA42.5% 56.4 / 12.8 40E174K (GAA→AAA) arnAfused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (18/5);  new base A (0/17);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 3.35e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTGAATGGCATAAACCGGCATCCGTACTGCACAACATGGTACGTGCC  >  NC_000913/2368410‑2368707
                                                                                                                                                    |                                                                                                                                                     
tGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGa                                                                                                                                                      >  1:655417/1‑150 (MQ=255)
    aCGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGa                                                                                                                                                 <  2:687860/151‑1 (MQ=255)
           cTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTAcc                                                                                                                                          >  2:722441/1‑151 (MQ=255)
             ggCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCg                                                                                                                                        <  2:238688/151‑1 (MQ=255)
             ggCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCg                                                                                                                                        <  1:423401/151‑1 (MQ=255)
                   aCATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCAtt                                                                                                                                   >  1:259354/1‑150 (MQ=255)
                    cATTGCACCGAATGGTAAAACGTGCGGAGGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATGGCCCCAGCCGATATCGTTATTACGCTGCATCAAAAATTGTGCCATGCCGCGCGCCAGCTACGGAACCAGACTTTCCCCGCCAt                                                                                                                                    <  1:36266/148‑1 (MQ=255)
                      ttGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAac                                                                                                                               >  2:778246/1‑151 (MQ=255)
                         cACCGAATGGTGAAGCGTGCCGATGCGGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATGTCTCTATTACGCTACATCAAAATTTGTGCCACGCGGCCCCCCAGCCCCTGGAAAAGACTTTACCCGCCATTAAaca                                                                                                                              <  1:817804/149‑1 (MQ=255)
                         cACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACg                                                                                                                            >  2:893898/1‑151 (MQ=255)
                                     aagcgggccgcggcgggggCTTTTGTGGCGCATCGGCGTTTCGCCAGGGCGCCAGCGGTTTTCTTTATCCCGCTGCATAAAAATTTGTCCCACCCCGCGCGCCGGCTGTGGAAACAGACTTTCCCCGCCATTAAACACGGTAATATTCTgg                                                                                                                <  1:452289/136‑1 (MQ=255)
                                     aaccGTGCCGAGGCGGGGCCCTTTGGGGCGCAACGGCGCTTTGCCTTTGCGCCAGACGATTTCGCTTTTCCGCTGCATAAAAAATTGTCCCCCCCCGCGCGCCAGCTACTGAAACAGACATTCCCCGCCATTAAACACGGTAATATTCt                                                                                                                  <  2:763101/146‑1 (MQ=255)
                                       aCGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGa                                                                                                               >  2:901175/1‑150 (MQ=255)
                                              gATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGccc                                                                                                       >  1:270850/1‑151 (MQ=255)
                                                    ggggCTTTTGGGGCGAAACTGCGCTTTGCCATGGCGCCAGACGATATCGCTTTTCCGCTGCATAAAAATTTGCCCCAGGCCGCGCCCCGGCTACGGAAACAGACATTCCCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGcgcg                                                                                                 <  2:44492/151‑1 (MQ=255)
                                                    ggggCCTTGGTGGCGCACCTGCGCATCCCCTTGCCCCCAGACTAAATCTCTATTACGCTACATAATAAAGGGTGCAACCCCGCGCGACAGCTAGGGGAACAGAATTTCCCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAg                                                                                                     <  1:159123/147‑1 (MQ=255)
                                                    gggcCCTTTGTGGCGAAACTGCCCTTTGCCATCGCGCCAGACGATACCGTTTTTCCGCTAAATAATAATTTGTCCCCGGCCGCGCCCCGGCTACGGAAACAAACATTCCCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAgc                                                                                                    <  2:73526/148‑1 (MQ=255)
                                                               gccgcAACGGCGCATTGCCTTTGCGCAAGATGTTATCGTTTTACCGCGAAATAAAAATTTGTGCCACGCCGCGCCCCAGCTTCGGAAAAAGACTTAACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACg                                                                                           <  2:853495/144‑1 (MQ=255)
                                                                    accggcgcTTTGCCATCGCGCAAGACAATACCGTTATTACGCTACATAAAAAATTGTGCCGTCCCGCCCGCCAGCTTCTGAAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGc                                                                                       <  2:105857/141‑1 (MQ=255)
                                                                     aCTGCGCATTGCCATTGCGCCAGAGAATATCTTTATTACCCTACATAATAATTTGTCCAGCGCCGCGCCCCATCTACGGAAACAGCCATCCCCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCt                                                                                  <  1:68439/149‑1 (MQ=255)
                                                                        gcgcATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGtttt                                                                             >  1:918663/1‑151 (MQ=255)
                                                                                      cgcCAGACAATATCTTTTTTCCGCTGAATAAAAATTTGTCCCAGGCCGCGCCCCAGCTCCGGAAAAAGACATCCCCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAAc                                                                  <  2:305573/148‑1 (MQ=255)
                                                                                         cAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCgg                                                             >  1:277018/1‑150 (MQ=255)
                                                                                                tatCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAg                                                      >  1:523548/1‑150 (MQ=255)
                                                                                                   cGCTTTTCCGCTGCATAAAAATTGGTGCCACGCCGCGCGCCGGCTACGGAAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTc                                                  <  2:341846/151‑1 (MQ=255)
                                                                                                      ttttcccctccatcaaAATTTGTGCCCTGCCGCGCGCCAGCTACTGAAACAGACTTTCCCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTcc                                                 <  1:265386/139‑1 (MQ=255)
                                                                                                      ttttcccctccaacatAAGTTGTCCCATCCCGCCCCCCAGCTAGTAAAACAGACTTTCCCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAg                                                      <  1:351931/131‑1 (MQ=255)
                                                                                                      tATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCtt                                               >  2:126682/1‑151 (MQ=255)
                                                                                                       aTTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTg                                              >  1:371467/1‑151 (MQ=255)
                                                                                                           cgcgaaataatAATTTGTCCCACCCCGCGCCCCAGCTACGGAAACAGACTTTCCCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTGaa                                            <  1:800935/140‑1 (MQ=255)
                                                                                                             cTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTGAATGGc                                        >  1:705775/1‑151 (MQ=255)
                                                                                                                 atcatAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTGAATGGCATa                                     >  2:13388/1‑150 (MQ=255)
                                                                                                                      aaaTTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTGAATGGCATAAACCgg                               >  1:614222/1‑151 (MQ=255)
                                                                                                                      aaaTTGTCCCACGCCGCGCCCCGCCTACGGAAAAAAACATCCCCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTGAATGGCATAAACCg                                <  1:394219/150‑1 (MQ=255)
                                                                                                                         tgggccccccccGCGCCCCAGCTACAGAAACAGACATCCCCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTGAATGGCATAAACCgg                               <  2:516658/138‑1 (MQ=255)
                                                                                                                              ccgggCCGCGCGCCTGTTCCGGAAAAAGACATTCCCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTGAATGGCATAAACCGGCATcc                          <  2:798812/144‑1 (MQ=255)
                                                                                                                                aTGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTGAATGGCATAAACCGGCATCCGTACt                     >  1:393928/1‑151 (MQ=255)
                                                                                                                                aTGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTGAATGGCATAAACCGGCATCCGTACt                     >  2:815076/1‑151 (MQ=255)
                                                                                                                                   ccgcccgcCACCTACGGAAACAGACATTCCCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTGAATGGCATAAACCGGCATCCGTACTGc                   <  2:464486/150‑1 (MQ=255)
                                                                                                                                    cgcgcgCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTGAATGGCATAAACCGGCATCCGTACTGcac                 >  1:50338/1‑151 (MQ=255)
                                                                                                                                                   ggAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTGAATGGCATAAACCGGCATCCGTACTGCACAACATGGTACGTGcc  >  1:157649/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACGCCGGATGACAGTTTCCTTGAATGGCATAAACCGGCATCCGTACTGCACAACATGGTACGTGCC  >  NC_000913/2368410‑2368707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑