Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	51.8%	70.0 / 101.1	56	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (2/25);  new base G (29/0);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 6.30e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.07e-09

ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGC  >  NC_000913/354138‑354435                                                                                                                                                        |                                                                                                                                                    aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGt                                                                                                                                                     <  2:205661/151‑1 (MQ=255)    ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGa                                                                                                                                                   <  2:53059/150‑1 (MQ=255)       gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGa                                                                                                                                               <  2:123297/151‑1 (MQ=255)             gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCt                                                                                                                                         <  1:152748/151‑1 (MQ=255)               gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGa                                                                                                                                       <  1:18350/151‑1 (MQ=255)                aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATg                                                                                                                                             >  1:488687/1‑144 (MQ=255)                 cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGaaa                                                                                                                                     <  2:908106/151‑1 (MQ=255)                  gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGaaaa                                                                                                                                                >  2:20241/1‑139 (MQ=255)                     gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGAAAggg                                                                                                                                  >  2:303521/1‑150 (MQ=255)                        ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAgg                                                                                                                              <  1:543383/151‑1 (MQ=255)                         gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggt                                                                                                                             <  1:12310/151‑1 (MQ=255)                         gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggt                                                                                                                             <  2:224870/151‑1 (MQ=255)                            aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGGAggggg                                                                                                                           >  2:418531/1‑150 (MQ=255)                              cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCAGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcg                                                                                                                         <  2:538139/150‑1 (MQ=255)                               aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggt                                                                                                                       <  2:920374/151‑1 (MQ=255)                                      tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGGGGGGGCGgtt                                                                                                                >  1:957971/1‑151 (MQ=255)                                       ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGAAGCGCTGGAAGGGCgggggggggggggg                                                                                                                   >  1:652518/1‑145 (MQ=255)                                         cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGAAGCGCTGGAAGGGGAggggggggtg                                                                                                                      >  1:728369/1‑142 (MQ=255)                                             cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGTTTGTCAtt                                                                                                          >  2:371055/1‑150 (MQ=255)                                              gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTcc                                                                                                        >  2:45412/1‑151 (MQ=255)                                                aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGTGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGATGCGGTGGAAGGGGAGGGGGCGGGGGGGGTTTTGATTCCg                                                                                                       >  1:780982/1‑150 (MQ=255)                                                   gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGAAA‑ATGTGGTGGAAGGGCGAGGGGCGGTGGCGGGTGTCCTTTCGaa                                                                                                     >  1:55275/1‑149 (MQ=255)                                                   gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaa                                                                                                     <  1:392526/149‑1 (MQ=255)                                                   gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAAATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTTATTCCGaaa                                                                                                    >  2:384591/1‑150 (MQ=255)                                                     cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGaaa                                                                                                    >  2:346328/1‑148 (MQ=255)                                                           aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                                                                                           <  2:488792/151‑1 (MQ=255)                                                            aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGAAGCGCTGGAAAGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAAAAGAGCg                                                                                             >  2:696948/1‑148 (MQ=255)                                                              tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg                                                                                         <  1:372783/150‑1 (MQ=255)                                                               aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTc                                                                                       <  2:711622/151‑1 (MQ=255)                                                               aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGGAAAAGAGCg                                                                                             >  2:801804/1‑145 (MQ=255)                                                                    tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGGGGGGGGGGGGGTTGTCCTTTCGGAAGAGGGCGGCAGTcctgg                                                                                   >  1:102767/1‑146 (MQ=255)                                                                     gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                                  <  2:253422/150‑1 (MQ=255)                                                                     gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                 <  2:902278/151‑1 (MQ=255)                                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCCGAAAAGGCAGGGGGCGGGGGGGGTTGTCCTTCCCAAAGAGAGCGACAGTCTg                                                                                     >  1:436560/1‑146 (MQ=255)                                                                                  tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGTGAAAGATGCGCTGGAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTgg                                                                     >  1:491930/1‑150 (MQ=255)                                                                                   ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                                   <  1:890960/151‑1 (MQ=255)                                                                                      gACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGTGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc                                                                <  2:781126/151‑1 (MQ=255)                                                                                       aCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCAc                                                                >  1:566443/1‑150 (MQ=255)                                                                                        cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGGGCACTc                                                              >  1:420931/1‑151 (MQ=255)                                                                                           gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGGGGCGGTTGTTATTTCGAAAGAGGGGGACAGTCTGGGAGATCGTGGAGTGGGGCACTCGc                                                            >  1:122576/1‑150 (MQ=255)                                                                                            cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAGA‑GCGCCGAAAGGGGAGGGGGCGGGGGCGTTTGGCATTTCGGAAGAGGGGGGCCGTCTGGAAGAACGTGGTGTGGGGGACTCg                                                             >  1:62690/1‑148 (MQ=255)                                                                                              agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCACTCGc                                                            >  2:446498/1‑147 (MQ=255)                                                                                               gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                       <  1:477757/151‑1 (MQ=255)                                                                                                    aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                  <  1:395682/151‑1 (MQ=255)                                                                                                     agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                                 <  2:264434/151‑1 (MQ=255)                                                                                                         agTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTa                                             <  1:830801/151‑1 (MQ=255)                                                                                                               tcCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGGTGGGGCGCACTCGCAAGAGAAGGCGATTATGgcg                                        >  1:359142/1‑150 (MQ=255)                                                                                                               tcCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGa                                                >  2:670200/1‑142 (MQ=255)                                                                                                                ccAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcgc                                       <  2:759281/150‑1 (MQ=255)                                                                                                                ccAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGCGCACTCGCAAGAGAAGGCGATTATGgcg                                        >  2:303248/1‑149 (MQ=255)                                                                                                                    ccATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtgggg                                  >  2:383570/1‑149 (MQ=255)                                                                                                                    ccATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGTGGGGTTTGTCATTCCGGAAGAGAGCGACAGTCTGGGAGATCGTGGTGTGGGGCACCCGCAAGGGAAGGCGGTTATGGCGCcggtg                                  >  1:958635/1‑151 (MQ=255)                                                                                                                              gTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAGATGCGCTGAAAGGGGACGGGGGGGGGGGGGTTGTGATTTCGAAAGAGAGGGACAGGCTGGGAGATCGGGAGGGGGCGGACTCGCCAGAGAAAGCGGTTATGGGGCtggggggc                               >  1:644479/1‑142 (MQ=255)                                                                                                                                 gCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCCGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGGGTGGCGCCCTCGCAAGAGAAGGGGATTATGGCGCTGGTGGACAGCCCGAtt                      >  1:910035/1‑150 (MQ=255)                                                                                                                                         ggCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACttt             <  1:336511/151‑1 (MQ=255)                                                                                                                                              t‑ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg        >  2:166342/1‑151 (MQ=255)                                                                                                                                                    ggggggAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTGGGCCGCCCg    <  1:358049/150‑1 (MQ=255)                                                                                                                                                     gggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgc  <  1:152699/151‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                    ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGC  >  NC_000913/354138‑354435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑