Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,581	0	A	G	44.1%	100.3 / 16.4	60	noncoding (72/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (2/31);  new base G (26/0);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 9.54e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAA  >  NC_000913/1097449‑1097728                                                                                                                                       |                                                                                                                                                          gTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGt                                                                                                                                       <  2:548743/151‑1 (MQ=40)        cAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGta                                                                                                                               <  2:421830/151‑1 (MQ=255)        cAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGG‑GGGGGGGGGGTTT‑GAAcccccccgaccttttc                                                                                                                                    >  1:3367/1‑137 (MQ=11)             ggCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATTCG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATacac                                                                                                                          <  1:159372/151‑1 (MQ=255)                tGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAAGCCCGTACTTTCGTACGTGCTCTTCTTTAAATATGGCGGG‑GAGGGGGGGGTTC‑GAACCCCCCCTA‑CGTTG‑CCGTTTacac‑a                                                                                                                        >  2:18395/1‑150 (MQ=14)                     ccAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCa                                                                                                                 <  1:282699/151‑1 (MQ=255)                       aGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGA‑TGGGGGGGGACAT‑GAAACCCCCACA‑CGGTT‑TCGTCTACAC‑ACtttactcg                                                                                                               >  1:136223/1‑146 (MQ=11)                        gTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTT‑GAACCCCCCCAA‑AGTTG‑CCGTATACAC‑ACCTTCCacg                                                                                                               >  2:132599/1‑148 (MQ=14)                        gTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTC‑GAACCCCCGATA‑CGTTT‑CCGGATACAC‑ACCTTTCAGGc                                                                                                              >  2:437578/1‑151 (MQ=255)                           gTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTg                                                                                                           <  2:121736/151‑1 (MQ=255)                               aaCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGG‑GAGGGGGGGGGTC‑GGAACCCCCATA‑CGTTG‑CCCGATAAAC‑ACATTCCAGGCGGGCTcc                                                                                                       >  1:717569/1‑151 (MQ=14)                                    cGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGGTC‑GAACCCCCCCTA‑CGGTT‑CCGTTTTCAC‑ACTTTTCCGGGGGGCTCCtt                                                                                                     >  1:623698/1‑148 (MQ=14)                                    cGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGA‑GAGGGGGGGGGTT‑GAAACCCCCCTA‑CGCTT‑CCGCATACAC‑CCATTTCCGGCGGGCTccct                                                                                                     >  1:544660/1‑146 (MQ=11)                                        cGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCAc                                                                                              <  2:169325/151‑1 (MQ=255)                                            gCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCgg                                                                                          <  1:913152/151‑1 (MQ=255)                                               tctcATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GGGGGGGGGGTTT‑GAACCCCCCCTA‑CGCTT‑CCGCTTTCAC‑ACCTTTCCCGCGGGCTCCCTCACCCCCTccg                                                                                          >  1:695103/1‑146 (MQ=11)                                               tctcATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGac                                                                                        <  2:692843/150‑1 (MQ=255)                                               tctcATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGac                                                                                        <  2:638804/150‑1 (MQ=255)                                                 tcATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTC‑GGACCCCCCGTA‑CGTTG‑CCGTATACAC‑ACTTTCCAAGCGCGCTCCCTCAGCCACTCGGACAcc                                                                                     >  2:443167/1‑151 (MQ=17)                                                  cATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGaca                                                                                       <  2:480810/148‑1 (MQ=255)                                                    tCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGGTC‑GAAAACCCGGGA‑CGTTT‑CCGGTTACAA‑ACTTTCCACGGGGGCTCCCTCTGCCACTCgggaaac                                                                                     >  1:325693/1‑143 (MQ=14)                                                        cccGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGGTC‑GGACCCCCCGTA‑CGCTG‑CCGGCTACACAACTTTCCA‑GCGGGGTCCTTCTGCCACCCCGGCAcccc                                                                                   >  2:402599/1‑144 (MQ=14)                                                        cccGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCaa                                                                              <  1:941973/151‑1 (MQ=255)                                                              gtgtgCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGt                                                                         <  1:608722/150‑1 (MQ=255)                                                                    aaTATACG‑ACAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGA‑GGGGGGGGGGTTT‑GCAACACCCATA‑CATTG‑TTGTCGAAAC‑ACCTTCCCAGGGTGGTCCTTTCTTCACCTGGACCCCTCAcc                                                                                >  2:390417/1‑137 (MQ=0)                                                                    aaTATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTT‑GAACCCCCCCTA‑CGTTT‑CCGGATACAC‑ACTTTTCCGGCGTGCCCCTTCAGCCCCCCGGGCACCTCCCCCAATTTTCG‑tt                                                                    >  2:321584/1‑148 (MQ=14)                                                                     atatACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GGGGGGGGGGTTC‑GAACCCCCCCTA‑ACCTT‑CCGGATAAAA‑CCTTTTCAGGCGGGGTCCTTCAACCCCCCCGACACCCCACCCAAATTTTG‑TTcc                                                                  >  2:278310/1‑149 (MQ=9)                                                                         aCG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑tt                                                           <  2:867052/151‑1 (MQ=255)                                                                         aCG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑t                                                            <  2:394135/150‑1 (MQ=255)                                                                         aCG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGG‑GAGGGGGGGGTTT‑GCACCCCCGAAA‑AGTTT‑GCGCCTTCAC‑ACATTTCAGGCGGGGCCCTTCCTCCCCCCGGACAACCCACCCAACTTTCGCTTCCttctt                                                             >  1:140909/1‑147 (MQ=2)                                                                          cG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCCTTAAATATGGCGGT‑GAGGGGGGGGTTC‑GAACCCCCCATA‑CCTTG‑CCGGATACAC‑ACCTTCCAGGCGTGCCCCTTCAGCCACTCCGACACCCCCCCAAAATTTCG‑TTCCTTTC‑tt                                                           >  2:41965/1‑150 (MQ=14)                                                                           g‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑TCTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGc                                                         <  2:885497/151‑1 (MQ=255)                                                                             aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCt                                                        <  2:337831/151‑1 (MQ=255)                                                                             aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGG‑GAGGGGGGGGATC‑GAAACCCCCCTA‑CGTTGACCGTTTCCA‑‑ACATTTCCGGCGGGCGCCCTTAGGCCCTCCGAGACCCCACCAAAATTTCG‑GTCCtctttt                                                            >  1:197168/1‑143 (MQ=2)                                                                                 aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGaa                                                    <  1:148215/151‑1 (MQ=255)                                                                                   aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACg                                                  <  2:273768/151‑1 (MQ=255)                                                                                      cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGgc                                               <  1:318117/151‑1 (MQ=255)                                                                                       ccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTCGGAACCCCCCA‑A‑CGTTT‑CCGGATACAC‑ACTTTCCCGGCGGGCTCCTTCAACCACCCCGACAACCCCCCCAATTTTTG‑TTTCTGTC‑TTTCTGGAACGGgcg                                              >  2:306114/1‑151 (MQ=9)                                                                                        cGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GGGGGGGGGGTTT‑GGACCCCCCCTA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGGGCTCCTTCCGCCACCCGGACACCCCCCCAAATTTTCG‑TTCCTGTC‑TTGCTGGAACGGgcg                                              >  1:616482/1‑150 (MQ=17)                                                                                         gTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GGGGGGGGGGTTT‑GGACCCCCGGTA‑CCTTT‑CCGTATTAAC‑ACTTTCCCGGCGGGCTCCTTCAGCCCCCCGGACACCCCCCCAAATTTTCG‑GTCCCTTC‑TTTTTTGAACGGgcg                                              >  1:513268/1‑149 (MQ=2)                                                                                          tACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGTTTC‑GAACCCCC‑CTA‑CGTTT‑CCCTATACAC‑ACTTTTCAGGCGGGCTCCCTCAGCCACCCGGACACCCCCCCCAATTATCT‑TTCCTGTC‑TTTCTGGAAAGGgcgc                                             >  1:127862/1‑149 (MQ=9)                                                                                                 gTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTT‑GAACCCCCCCCACCGTTG‑‑CGTATACAC‑ACTTTCCAGGCGTGGTCCTTTAGCCCCCCGGACACCCCCCCCAATTTTCG‑TTTCTTTC‑TTGCTGGGACGGGCGCTCAttt                                       >  1:774723/1‑148 (MQ=9)                                                                                                 gTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTT‑GAAACCCCCCTA‑CGTTT‑CCGGATAAAA‑ACTTTTCAGGCGTGCTCCTTCAGCCACCCCGACACCCCCCCCAAATTTCG‑GTTCTGTCTTTTTTGGAAC‑GGCGCTAAttt                                       >  2:922305/1‑148 (MQ=2)                                                                                                  tACGAGCTCTTCTTTAAATATGGCGGT‑GGGGGGGGGGGTT‑GAAACCCCCCCA‑CGTTT‑TCGTATACAC‑ACTTTCCAGGCGGGCTCCCTCCGCCCCCCCGGCACCCCCCCAAAATTTCG‑TTCCTTTC‑TTTCTTGGACCGGCGCTCATTTAggg                                   >  1:663159/1‑151 (MQ=9)                                                                                                   aCGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGa                                  <  2:944265/151‑1 (MQ=255)                                                                                                    cGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGaa                                 <  1:648462/151‑1 (MQ=255)                                                                                                      aGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTC‑TAAACCCCCATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTTCGCCCCTCCGACACCCCACCAAAATTTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTCATTTTGGGgaa                                >  1:848053/1‑150 (MQ=17)                                                                                                          cttcttTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGAAATCa                             <  1:741859/149‑1 (MQ=255)                                                                                                          cttcttTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGAAATCa                             <  2:326059/149‑1 (MQ=255)                                                                                                           ttcttTAAATATGGCGGT‑GAGGGGGGGGTTT‑TAACCCCCCCTA‑CGTTG‑CCGTATACAC‑ACTTTTCCGGCGGGCTCCTTCCGCCACCCCGACACCCCACCCAAATTTCG‑TTCCTGTC‑TTTCTGGAAAGGGCGCTAATTTTGGGAAAtta                             >  2:832712/1‑146 (MQ=17)                                                                                                            tcttTAAATATGGCGGT‑GAGGGGGGGGGTT‑GAACCCCCCCTA‑ACTTT‑CCGTATAAAC‑ACCTTCCCGGCGGGCTCCCTCAGCCCCCCGGGCACCCCCCCCAAATTTTG‑TTTCTTTC‑TTGCTGGGACGGGCGCTAAATTTAGGAAAACATg                           >  2:17157/1‑149 (MQ=9)                                                                                                            tcttTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGAAATCAt                            <  1:665382/148‑1 (MQ=255)                                                                                                                 aaaTATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAg                    <  1:545440/151‑1 (MQ=255)                                                                                                                   atatGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGt                  <  1:768571/151‑1 (MQ=255)                                                                                                                        gCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaaca             <  2:899208/151‑1 (MQ=255)                                                                                                                            t‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACt         >  1:529311/1‑151 (MQ=255)                                                                                                                              gAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt        <  1:880419/151‑1 (MQ=255)                                                                                                                                  gggggATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTg    <  2:719090/151‑1 (MQ=255)                                                                                                                                  gggggATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTg    <  1:18392/151‑1 (MQ=255)                                                                                                                                    gggATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaa  <  2:30263/151‑1 (MQ=255)                                                                                                                                       |                                                                                                                                                          GTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAACCCCCGATA‑CGTTG‑CCGTATACAC‑ACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTC‑TTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAA  >  NC_000913/1097449‑1097728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑