Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	701,889	IS1 (+) +9 bp	coding (855‑863/1149 nt)	nagA ←	N‑acetylglucosamine‑6‑phosphate deacetylase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	78 (0.910)	59/294	0.6	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	701889 =	0 (0.000)					coding (863/1149 nt)	nagA	N‑acetylglucosamine‑6‑phosphate deacetylase
*	?	NC_000913	= 258675	NA (NA)	84 (0.970)	65/298	0.4	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 701897	0 (0.000)					coding (855/1149 nt)	nagA	N‑acetylglucosamine‑6‑phosphate deacetylase

AAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTC  >  NC_000913/257770‑257918                                                                                                                                           |           aaaaaTTTGTTAAATTTTGCCGATTAGTTATATCAGTTGCATCGCATCAGGAGATAGCAATGATCTTTCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATCTTCCTGAaggtacgtgcaaagat  <  2:984164/149‑9 (MQ=255)                                                                                                                                           |           AAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTC  >  NC_000913/257770‑257918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.