Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,421,443	0	A	C	46.2%	144.3 / 19.6	91	Y40S (TAC→TCC)	folX	D‑erythro‑7,8‑dihydroneopterin triphosphate 2'‑epimerase and dihydroneopterin aldolase
Reads supporting (aligned to +/- strand):  ref base A (43/6);  new base C (0/42);  total (43/48)
Fisher's exact test for biased strand distribution p-value = 7.78e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGC  >  NC_000913/2421297‑2421584                                                                                                                                                    |                                                                                                                                              tCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAGTTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTAcccc                                                                                                                                           >  2:614156/1‑151 (MQ=255)   cTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCccgc                                                                                                                                         >  2:768937/1‑151 (MQ=255)     aaGTAAAACAACGGAGACCCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCccgccg                                                                                                                                       <  2:15446/151‑1 (MQ=255)     aaGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACACCCGTCAGGATATTGTTATCAATGTGACGATCCACTACCccgccg                                                                                                                                       <  2:267582/151‑1 (MQ=255)     aaGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCccgc                                                                                                                                         <  1:1238525/149‑1 (MQ=255)              aacGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAgcgc                                                                                                                              >  2:1034894/1‑151 (MQ=255)              aacGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAgcgc                                                                                                                              >  2:504333/1‑151 (MQ=255)                    gaACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACt                                                                                                                         >  2:934578/1‑150 (MQ=255)                     aaCCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACt                                                                                                                         >  1:562316/1‑149 (MQ=255)                            aaggccacacccgcccGTTATTATTCGAAAAAAGACCCTTCTTTTGCGTACTTTTATCGAAATTAAGA‑AAAAAAAAATAAACACCCGTCAGAATTTTTTTATCAGTGGGCCGATCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGat                                                                                                                <  1:1060106/137‑1 (MQ=255)                            aaggccacaCCCTGCCGTTTTTATTCGTATAAAGACCCTTCTTTTGCGTTCTTTTATGGGAATAAAGG‑AAAAAAAAATAAACACCCGTCAGGATTTTTTTATCAATGTGACTACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGa                                                                                                                    <  1:1283315/142‑1 (MQ=255)                              tGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGatat                                                                                                              >  2:225473/1‑151 (MQ=255)                                acacaACCTCCCTTTATTATTCGAATAAAGAACTTTCGTTTGCGTTGTTTTATCGAATGAAAGA‑AAGAAAAAATCAACACCCGTAGGGATGTTTTTAACGATGGTACACCCCGCGCCCCCGCCGATAAAGCGCGCACTAGCGAAGatat                                                                                                              <  2:350394/148‑1 (MQ=255)                                  acacCCTGCCGTTATTATTGAAAAAAAGAACTTTCTTTTGCTTACTTTTATGGAAATAAAGA‑AAGAAAAAATTAACACCCGGAAGTATATTTTTACAAATGTGACTTCCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGata                                                                                                               <  1:1213449/146‑1 (MQ=255)                                   caACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACg                                                                                                         >  2:208973/1‑151 (MQ=255)                                     cccTCCCGTTATTTTTATTAAAAAGAACCTTCTTTTGCGTCCTTTTATCGGAATAAAGG‑AAGAAAAAATAACCACCCGTCAGGATATTTTTATCAATGTGACCATCCACCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGa                                                                                                        <  1:693970/149‑1 (MQ=255)                                      ccgcccgtttttattaGAAAAAAAACCCTTCTTTTGCGTCCTTTTATCGAAATAAAGA‑AAAAAAAAAAAAACACCCGAAAGGTTTTTTTTAAAAAGGTGCCGACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCa                                                                                                            <  1:226060/135‑1 (MQ=255)                                      ccTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATg                                                                                                      >  2:1096325/1‑151 (MQ=255)                                        tcctgCTATTTTTCATAAAAAGAACCTTGTTTTGCTTACGTTTACAGAAATAAAGG‑AAAAAAAAATAAACACCCGTCAGTTTATTTTAACAAATGTGCCCCTCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATgc                                                                                                     <  1:1348816/146‑1 (MQ=255)                                        tGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATgcg                                                                                                    >  2:851437/1‑151 (MQ=255)                                          ccGTTATTTTTCGAAAAAAGACCCTTCTTTTGCGTACTTTTATCGAAATAAAGA‑AAAAAAAAATAAACACCCGTCAGGATATTTTTAACAATGTGACGATCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCt                                                                                                  <  1:34993/151‑1 (MQ=255)                                          ccGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCt                                                                                                  >  2:233019/1‑151 (MQ=255)                                           cGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTg                                                                                                 >  2:1192544/1‑151 (MQ=255)                                            ccTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGACTTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTg                                                                                                 >  2:1024308/2‑150 (MQ=255)                                                ttattCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAAtt                                                                                             >  1:240095/1‑150 (MQ=255)                                                ttattCGTAAAAAGACCTTTCTTTTGCGTACGTTTATC‑GAATTAAGGAAAAAAAAAATAAACACCCGTCGGGATTTTTTTATAATTGTGCCGACCCCCCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGaa                                                                                               <  1:537462/148‑1 (MQ=255)                                                 tattgttaaaaaGACCCTTCTTTTGCGTTTGTTTATGGAAATTAAGA‑AAAAAAAAAAAACCACCCGTAAGGATATTTTTATAAAGGTGACAACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTa                                                                                            <  2:509869/141‑1 (MQ=255)                                                 tattagaaaaaaGACCCTTCGTTTGTTTTCTTGTATCGAAATAAAGG‑AAAAAAAAATAAACACCCGTAAGGATATTTTTAACAATGTGACACCCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTa                                                                                            <  2:1042574/141‑1 (MQ=255)                                                 tattCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTAt                                                                                           >  1:393779/1‑151 (MQ=255)                                                   ttCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCg                                                                                         >  2:1042773/1‑151 (MQ=255)                                                   ttCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCg                                                                                         >  1:365754/1‑151 (MQ=255)                                                    tCGTAAAAAGACCTTTCGTTTGCGTACTTTTATCGGAATTAAGG‑AAAAAAAAATTAACACCCGTCAGGATATTTTTATCAATGTGACGATCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCg                                                                                         <  1:412516/150‑1 (MQ=255)                                                    tCGTAAAAAGAACCTTTTTTTGCGTCCGTTTACGGAAATTAGGA‑AAAAAGAAATAAACAGCCGGCAGTATATTTTTAAAAATGTGCCGACCCCCGACGCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATc                                                                                          <  1:1114188/149‑1 (MQ=255)                                                     cttaaaaaaacccTTCTTTTGCTTCCGTTTATCGAAATAAAGA‑AAAAAAAAATTAACACCCGTCAGGATATTTTTATAAAGGTGACGACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCg                                                                                         <  1:834694/138‑1 (MQ=255)                                                     cataaaaaGACCCTTCTTTTGCGTACTTTTATCGAAATAAAGA‑AAAAAAAAATAAACACCCGACAGGATATTTTTATAAATGGGACGACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCa                                                                                       <  1:30478/146‑1 (MQ=255)                                                     cGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGc                                                                                        >  1:1280924/1‑150 (MQ=255)                                                     cGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGc                                                                                        >  2:1084039/1‑150 (MQ=255)                                                          aaacacttttCGTTGGCGTACGTTTTTGGGAATAAAGG‑AAGAAGAAAATAACACCGGTAAGGATTTTGTTATCAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTa                                                                                  <  2:589193/143‑1 (MQ=255)                                                              acccTTCGTTTGCTTTGTTTTATCGAAATAAAAA‑AAAAAAAAATAACCACCTGTCAGGTTTTTTTTACAAATGTGACCATCCACCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACg                                                                               <  2:997249/148‑1 (MQ=255)                                                              aaCCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACg                                                                               >  1:535139/1‑150 (MQ=255)                                                              aaCCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCAACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACg                                                                               >  2:253408/1‑150 (MQ=255)                                                                 cTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGaaa                                                                            >  2:1302651/1‑150 (MQ=255)                                                                    cGTTTGCGTACGTTTATCGGAATTAAGG‑AAGCAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAAc                                                                         >  2:11743/1‑150 (MQ=255)                                                                    cGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAAc                                                                         >  1:997582/1‑150 (MQ=255)                                                                    cGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAAc                                                                         >  1:834281/1‑150 (MQ=255)                                                                      tttGTGTATTTTTAT‑GGAAATAAGGAAAAAAAAAATAAACCCCGGTCAGAATTTTTTTATCAGTGAGCCACTCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGa                                                                              <  1:1175010/143‑1 (MQ=255)                                                                      tttGCGTACTTTTATAGAAATAAAGA‑AAAAAAAAATAAAAACCCGCAAGGATTTTGTTAACAATGTGACTACCCTCCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGaaaaa                                                                          <  1:731263/147‑1 (MQ=255)                                                                      tttGCGTACTTTTAGCGAAATAAAGA‑AAAAAAAAATAAACACCGGTCAGAATTTTGTTATCAATGTGACGCCCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGaaaa                                                                           <  2:1144910/146‑1 (MQ=255)                                                                      tttGCGTACGTTTATCGAAATAAAGA‑AAGAAAAAATTAACACCCGTCAGAATATTTTTATCAATGTGACGATCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGaaaa                                                                           <  1:501069/146‑1 (MQ=255)                                                                      ttggCGTACGTTTATGGAAATTAAGG‑AAGAAAAAATTAACACCGGAGGGTATTTGTTTAAAAAGGTGACGCTCCACCACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACa                                                                        <  2:975363/146‑1 (MQ=255)                                                                        tGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACattat                                                                    >  1:674059/1‑151 (MQ=255)                                                                                tttATGAGAATAAAGA‑AAGAAAAAATAAACACCCGTCAGTATATTGTTAACAAGGTGACTACCCACCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCa                                                                 <  1:1088566/146‑1 (MQ=255)                                                                                 ttATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGt                                                           >  2:751130/1‑151 (MQ=255)                                                                                     gggAATAAAGA‑AAAAAAAAATAACCACCCGAGAGAATTTTTTTATAAATGTGACGACCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTagag                                                       <  2:426147/150‑1 (MQ=255)                                                                                     cgaaaTTAAAA‑AAGAAAAAATAAACACCGGTGAGGTTATTTTTATCATTGTGTCCTTCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCAt                                                             <  2:789936/142‑1 (MQ=255)                                                                                     cGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTag                                                         >  1:989207/1‑149 (MQ=255)                                                                                        aaTAAAGA‑AAAAAAAAATAAACACCCGTAAGGATATTTTTATAAATGGGACGACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTagag                                                       <  1:769495/148‑1 (MQ=255)                                                                                         ataaaGA‑AAAAAAAAATAAACACCCGAAAGGATTTTTTTATAAATGTGACGCCCCCCACCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGaa                                                     <  1:67007/146‑1 (MQ=255)                                                                                         aataaaaaaagaaaaaATAAACACCCGTCAGGATTTTGTAATAAATGTGACAACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTa                                                          <  2:1238221/137‑1 (MQ=255)                                                                                             aGG‑AAGAAAAAATTAACACCCGTCAGGATTTTTTTATCAATGTGACGATCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCg                                               <  1:208706/151‑1 (MQ=255)                                                                                                 aaaaaaaaaacaacaccCGTCAAGATATTTTTATAAAGTTGACCATCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGttt                                            <  2:314321/140‑1 (MQ=255)                                                                                                  agaagaaATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTtc                                           >  1:292122/1‑151 (MQ=255)                                                                                                   aaaaaaaTAAACACCCGTCAGAATATTATTATCAATGTGACCCTCCACACCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTtct                                          <  2:284934/147‑1 (MQ=255)                                                                                                    aaaaaaTTAACACCCGTCAGAATATTTTTATAAAGGTGACGACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTtct                                          <  1:504448/147‑1 (MQ=255)                                                                                                      aaaaTAAACCCCCGTAAGATTATTTTTAACAATGTGACCCCCCCTCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCtt                                       <  2:572527/150‑1 (MQ=255)                                                                                                       aaaTTAACACCCATAAGTATTTTTTTAACAATGTGACGATCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCttt                                      <  2:55994/151‑1 (MQ=255)                                                                                                           tAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTg                                  >  1:590555/1‑151 (MQ=255)                                                                                                           tAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACACCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGCTGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTg                                  >  2:870232/1‑151 (MQ=255)                                                                                                            accacccGTCAGGATTTTTTTATAAATGTGACGCCCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTg                                  <  1:935203/145‑1 (MQ=255)                                                                                                             acaacCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTgg                                 >  1:1300845/1‑150 (MQ=255)                                                                                                              cacccGTCGGTATTTTTTTACAAATGTGCCGCCCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGa                                <  1:927797/147‑1 (MQ=255)                                                                                                              caacCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGa                                >  2:279813/1‑150 (MQ=255)                                                                                                              caacCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGa                                >  1:630632/1‑150 (MQ=255)                                                                                                                 ccGTCAGGATATTTTTATAAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGaaaa                             <  1:948399/150‑1 (MQ=255)                                                                                                                  cGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAAt                           >  1:337349/1‑151 (MQ=255)                                                                                                                   gacaagATATTGTAAACAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGaaaaa                            <  2:838159/144‑1 (MQ=255)                                                                                                                   gTCAGGATATTGTTATAAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAAtt                          <  1:233190/151‑1 (MQ=255)                                                                                                                     cAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTa                         >  1:50030/1‑150 (MQ=255)                                                                                                                       ggATTTTTTTTTAAATGTGCCGTCCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAAtt                          <  1:109342/147‑1 (MQ=255)                                                                                                                         atatTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCCCTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAaactaact                      >  2:1129870/1‑149 (MQ=255)                                                                                                                         atatTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTc                     >  1:1173192/1‑150 (MQ=255)                                                                                                                          ttttGTTATAAATGTTACGACCCTCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCa                    <  2:1190640/148‑1 (MQ=255)                                                                                                                          tatTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTc                     >  2:863325/1‑149 (MQ=255)                                                                                                                           atTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAgg                  >  1:323876/1‑151 (MQ=255)                                                                                                                             tGTTAGAAAGGTGACGATCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAgg                  <  2:505447/149‑1 (MQ=255)                                                                                                                              gTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGAtg               >  1:383203/1‑151 (MQ=255)                                                                                                                                    aaggtgACGACCCTCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGAtgtg             <  2:314782/144‑1 (MQ=255)                                                                                                                                    aaTGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCg         >  2:890290/1‑151 (MQ=255)                                                                                                                                        tgACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGatat     >  1:939806/1‑151 (MQ=255)                                                                                                                                           cgctCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGc  <  2:1100586/148‑1 (MQ=255)                                                                                                                                             acccACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACACTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGatat     <  2:562189/144‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              TCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGG‑AAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGC  >  NC_000913/2421297‑2421584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑