Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
MC JC | NC_000913 | 3,824,201 | Δ6 bp | coding (1802‑1807/2109 nt) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
Missing coverage evidence... | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 3824201 | 3824206 | 6 | 61 [0] | [0] 61 | spoT | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
New junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 3824200 | 0 (0.000) | 61 (0.950) | 59/298 | 0.2 | 100% | coding (1801/2109 nt) | spoT | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
? | NC_000913 | 3824207 = | 0 (0.000) | coding (1808/2109 nt) | spoT | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
CCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTC > NC_000913/3824061‑3824354 | ccaaagccacggacatctgcccattaaaggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGc > 1:525549‑M2/147‑151 (MQ=255) caaagccacggacatctgcccattaaaggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCt < 2:605508‑M2/6‑1 (MQ=255) ccacggacatctgcccattaaaggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTAcc > 2:933844‑M2/141‑149 (MQ=255) acggacatctgcccattaaaggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGaa < 1:32292‑M2/13‑1 (MQ=255) gacatctgcccattaaaggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAaga < 2:846067‑M2/16‑1 (MQ=255) acatctgcccattaaaggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAagag < 1:287329‑M2/17‑1 (MQ=255) catctgcccattaaaggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGc > 2:266365‑M2/134‑151 (MQ=255) atctgcccattaaaggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGcc > 2:337746‑M2/133‑151 (MQ=255) gcccattaaaggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCaga < 1:851047‑M2/22‑1 (MQ=255) gcccattaaaggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCag < 1:397316‑M2/21‑1 (MQ=255) ccattaaaggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAgaga > 1:886555‑M2/127‑150 (MQ=255) ccattaaaggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAgaga > 2:934300‑M2/127‑150 (MQ=255) ccattaaaggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGaa < 1:734796‑M2/25‑1 (MQ=255) cattaaaggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAgaga > 1:595636‑M2/126‑149 (MQ=255) aggtgccgatggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATg > 2:941388‑M2/120‑151 (MQ=255) gatggcgcgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaacggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCCGTGAAGTTTATGGCTGTgg > 2:15152‑M2/113‑151 (MQ=255) tggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTgg > 2:836387‑M2/111‑149 (MQ=255) ggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAAt > 1:885439‑M2/110‑151 (MQ=255) ggcgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAAt < 1:274911‑M2/42‑1 (MQ=255) cgtgctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATgg < 2:937061‑M2/44‑1 (MQ=255) ctgatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGAt > 1:700847‑M2/104‑150 (MQ=255) gatcacctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATaaa < 2:558483‑M2/50‑1 (MQ=255) cctttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgttccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGTGAAGTTTATGTCTGCGGAATGGGCGAAag > 2:424268‑M2/97‑147 (MQ=255) ttgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACg < 1:8163‑M2/56‑1 (MQ=255) tgcgaaatgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGgcg > 1:46374‑M2/93‑151 (MQ=255) atgctgccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAgg > 1:362511‑M2/87‑149 (MQ=255) ccgccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTtcatc > 1:896299‑M2/81‑151 (MQ=255) ccctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCAcc > 1:914946‑M2/78‑151 (MQ=255) cctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCg > 1:589179‑M2/77‑151 (MQ=255) ctattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCg > 1:584543‑M2/76‑150 (MQ=255) tattcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGa > 1:733524‑M2/75‑150 (MQ=255) attcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGaa > 1:708485‑M2/74‑150 (MQ=255) attcctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGaa < 1:172980‑M2/77‑1 (MQ=255) cctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATc > 2:382553‑M2/71‑150 (MQ=255) cctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCa < 2:558031‑M2/81‑1 (MQ=255) ctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATc > 2:965521‑M2/70‑149 (MQ=255) ctggcgacccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATc < 1:182480‑M2/80‑1 (MQ=255) gacccgattatcgcgcccgtcagccccggtaaaggtctggtgatccaccatgtatcctgccgtaGTGGCTACCCGAAGGAGCCCGAGAAGTTTATGGCTGTGGTATGGGATAAAGGGACGGCGCAGGAGTTCATCACCGAAATCAAGGCgg > 2:51956‑M2/65‑151 (MQ=255) cccgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGCAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTgg > 2:267608‑M2/63‑149 (MQ=255) cgattatcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGAt < 1:862348‑M2/91‑1 (MQ=255) atcgcccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTc > 2:574019‑M2/56‑150 (MQ=255) cccacgtcagccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAatca < 1:657732‑M2/100‑1 (MQ=255) agccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTg > 1:88319‑M2/44‑151 (MQ=255) agccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTg < 1:22621‑M2/108‑1 (MQ=255) gccccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTgc < 1:675971‑M2/109‑1 (MQ=255) ccggtaaaggtctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCt < 2:818545‑M2/112‑1 (MQ=255) tctggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTg > 1:846688‑M2/30‑151 (MQ=255) tggtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGAc > 1:646234‑M2/28‑151 (MQ=255) gtgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGAcgg < 1:898860‑M2/126‑1 (MQ=255) tgatccaccatgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGCAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGAcggc > 2:848623‑M2/25‑151 (MQ=255) catgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGTTAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGTCAAt < 2:456498‑M2/133‑1 (MQ=255) catgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCaa > 2:447144‑M2/17‑148 (MQ=255) catgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTa < 1:389606‑M2/135‑1 (MQ=255) tgaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAac > 1:683074‑M2/15‑151 (MQ=255) gaatcctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGGGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAac > 2:877007‑M2/14‑150 (MQ=255) cctgccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAAcaccac < 2:886229‑M2/142‑1 (MQ=255) cctgccgtaGGGGCTACCAGAAAGAGCAAGAGAAGTTTATGGCTGGGGAATGGGATAAAGAGACGGCGCAGGAGAGAATCACCGAAATCAAGGGGGAGAGGTTCAATCAACAGGGAGCGCTGGCAAACCTGACGGCGGCAATTAAcacca > 2:506485‑M2/10‑150 (MQ=255) ccgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACt < 2:733242‑M2/146‑1 (MQ=255) cgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACtt > 1:792148‑M2/5‑151 (MQ=255) cgtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACtt > 2:595693‑M2/5‑151 (MQ=255) gtaGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTc < 2:602505‑M2/148‑1 (MQ=255) | CCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTC > NC_000913/3824061‑3824354 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |