Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	41.4%	79.1 / 44.0	70	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (5/36);  new base T (29/0);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 6.87e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206289‑3206585                                                                                                                                                    |                                                                                                                                                        gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAg                                                                                                                                                      <  1:252872/150‑1 (MQ=255)   aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACa                                                                                                                                                   <  1:528777/151‑1 (MQ=255)      tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTTAGACaaa                                                                                                                                                 >  2:500550/1‑150 (MQ=255)       cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGAaaaa                                                                                                                                                 >  1:440689/1‑149 (MQ=255)       cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACaaa                                                                                                                                                 >  1:28198/1‑149 (MQ=255)          aaacCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACaaaaa                                                                                                                                               >  1:547546/1‑146 (MQ=255)          aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAGTTTTTTTAGACAAATa                                                                                                                                               >  2:783530/1‑148 (MQ=255)          aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGAaaaa                                                                                                                                                 >  1:547562/1‑146 (MQ=255)            aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACaaaa                                                                                                                                          <  1:454845/151‑1 (MQ=255)            aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACaaaa                                                                                                                                          <  2:132515/151‑1 (MQ=255)               aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACt                                                                                                                                       <  2:703606/151‑1 (MQ=255)                 tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATACaaaaa                                                                                                                                         >  1:417359/1‑147 (MQ=255)                     aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGAAAAAAACAAAAAATGGA‑ggt                                                                                                                                >  1:287697/1‑149 (MQ=255)                       tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTg                                                                                                                               <  2:361957/150‑1 (MQ=255)                         ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCCGAACAAAATAAAAAATGGG‑GTTGccc                                                                                                                            >  1:308498/1‑150 (MQ=255)                         ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAAAGGA‑GGTGcc                                                                                                                             >  1:900742/1‑150 (MQ=255)                             ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAACGGAGGTTGcctg                                                                                                                           >  1:945268/1‑147 (MQ=255)                             ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACAAATTAAAAAAATGGA‑GTTTGCatgtt                                                                                                                        >  2:145636/1‑149 (MQ=255)                                 ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCatgatg                                                                                                                       <  2:126799/148‑1 (MQ=255)                                 ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCAtgatga                                                                                                                      <  1:721963/149‑1 (MQ=255)                                 ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGc                                                                                                                    <  1:282953/151‑1 (MQ=255)                                  gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GAAATTTTTCAAACAAAAAAAAAAACTGGA‑GTTTGCatgatgt                                                                                                                      >  2:348882/1‑147 (MQ=255)                                     cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTTCA‑ACAAAAACAAAAAATGGA‑Gttttcaatatg                                                                                                                       >  2:573474/1‑135 (MQ=255)                                     cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATACAAAAAATGGA‑GGTGCCATGATGAGCg                                                                                                                   >  1:760611/1‑148 (MQ=255)                                     cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGaaa                                                                                                                <  2:501838/151‑1 (MQ=255)                                       atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGt                                                                                                              <  2:157880/151‑1 (MQ=255)                                       atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGt                                                                                                              <  2:971499/151‑1 (MQ=255)                                        tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCAACAAAAAAAAAAAATGGA‑GGTGCCCTGATGAGGGaaa                                                                                                                >  1:296648/1‑148 (MQ=255)                                             attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAAATGA‑GTTGCCATGGTGAGCGAAAGgaataa                                                                                                         >  1:659318/1‑150 (MQ=255)                                               tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGc                                                                                                       <  1:512685/150‑1 (MQ=255)                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATACAAAAAATGGA‑GTTGTCTTGATGAGCGAAAGTAATAAACaa                                                                                                     >  2:156124/1‑149 (MQ=255)                                                     tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGGA‑GGTGCCATGATGAGCGAAAGTAAAAAACaaaa                                                                                                   >  1:777216/1‑146 (MQ=255)                                                       tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                               <  1:583801/150‑1 (MQ=255)                                                              gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                        <  1:560816/150‑1 (MQ=255)                                                                ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAATTGA‑GGTTCCAAGATGAGCGAAAATAAAAAAGAAAAAGCAGGGAATaa                                                                                       >  1:271659/1‑149 (MQ=255)                                                                   aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGa                                                                                  <  1:732930/151‑1 (MQ=255)                                                                    cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAc                                                                                 <  2:553042/151‑1 (MQ=255)                                                                     gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                                <  2:422566/151‑1 (MQ=255)                                                                     gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                                <  1:428729/151‑1 (MQ=255)                                                                     gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GAGTTTTTTTAAACCAAAAAAAAAAATGGA‑GGTTTCATGGTGAGGGAGAGTAATAAGAAAAAGGCAGGGAATAAGtt                                                                                    >  2:602250/1‑147 (MQ=255)                                                                      aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACag                                                                               <  1:996262/151‑1 (MQ=255)                                                                      aaCTATTAATTACATATTCACTGTTGCAAAAAATATAAAACCACATTTTTATTTGTAGTTTGTATCAGT‑TAATTTTTCCATACAAATACAAAAACTAAA‑GTTTCCATAATGAGCTAAAATAATAAGCAACAATCAGTTAATAATTTAACa                                                                                <  2:297776/150‑1 (MQ=255)                                                                              aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAAAGGA‑GGTGGCATGATGAGGGAAAAGAATAAAAAAAAAGCAGGGAAAAAATTGACAgaga                                                                            >  1:438828/1‑146 (MQ=255)                                                                                tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                      <  1:499619/150‑1 (MQ=255)                                                                                     atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGGA‑GGTGCCATGATGAGCGAAAATAATAAACAACAAGCAGTGAAAAAGTTGACAGAGATTGTCGCTaa                                                                  >  1:838344/1‑149 (MQ=255)                                                                                        cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAAACAAAAAAAAAAAATGGA‑GTTGGCAAGATGAGCGAAAGTAATAAAAAAAAAGGAGTGAAAAAGAGTACAAAGATTGTCGCCaaaaat                                                              >  2:295305/1‑146 (MQ=255)                                                                                              ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                                       <  1:114825/151‑1 (MQ=255)                                                                                                cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAAACAAAAAAAAAAAATGGA‑GTTTTCATGGTGAGGGGAAGGAATAAACAAAAAGCAGAGAATAAAATTAAAGAGATTGTCGCTAACCTTACCGcc                                                        >  2:287707/1‑148 (MQ=255)                                                                                                 aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCAt                                                      <  2:375563/149‑1 (MQ=255)                                                                                                   aaaaaaTAAACCCACATTTTTAGGGGTAGTTTGGGGCGGT‑GAATTTTCCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTTCCGCCATGAtt                                                  <  2:178678/151‑1 (MQ=255)                                                                                                   aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACGGGA‑TTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTAACAGAGATGGTCGCTAACTTTACCGCCATGAtt                                                  <  2:287525/151‑1 (MQ=255)                                                                                                    aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAAAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAttt                                                 <  1:604005/151‑1 (MQ=255)                                                                                                     aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GAATTTTTTCAGAAAAAAAAAAAAAATGGG‑GTTGTCATGATGAGCGGAAGGAATAAACAAAAAGCAGAGAAAAAAATGAAAGAGATTTTTGCTAAATTTACCCCCCTGAtttt                                                >  2:559230/1‑150 (MQ=255)                                                                                                       tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAAACAAAATAAAAAACAGGA‑GGTTCCATGGGGAGAGGGAATAATAAACAACAAGGAGTGAATAAGGTTACCGAGGGTGTCGGTAAACTTACCCCCCTTGTTTCt                                               >  2:580921/1‑150 (MQ=255)                                                                                                        ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑TAATTTTTTCAGAAAAAAACAAAAACTGGA‑GGTGCCCTGATGAGCGCAAGTAATAAACAAAAGGCAGAGAATAAATTGATAGAGAATTTTGCTAACTTTACACCCATGAtt                                                  >  2:396608/1‑146 (MQ=255)                                                                                                              ccACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAAAAATAAAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                                       <  1:430251/151‑1 (MQ=255)                                                                                                               cacaTTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTCCCGACAAATACAAAAAATGGA‑GGTGGCATGATGGGCGGAAGGAATAAACAAAAAGCCGTGGAAAAGTTGAAAGAGATTGTCGGCAAATTTACCCCCATGATTTTTTCCCCaa                                        >  1:233009/1‑149 (MQ=255)                                                                                                               cacaTTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAAAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCGGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATg                                      <  2:993365/151‑1 (MQ=255)                                                                                                                   tttttAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCTCACAAATACCAAAAATGGA‑GTTGTCCTGATGAGGGAAAATAATAAACAACAGGCCGAGGATAAAGTTACAAAGATTGTCGCTAACTTTACAGCCACGATTTTTACCCGaa                                        >  2:722380/1‑145 (MQ=255)                                                                                                                   ttttaAGTGGTAGTTTGTGGGGGT‑GAATTTTTCCAAAAAAATAAAAAACCTGGA‑GTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGGAAATAAGTTGCAAGAGATTGTCCCTAATTTTACCGCCATGATTTCTACCCGAATGCCt                                   <  2:296457/150‑1 (MQ=255)                                                                                                                     tttAGTGGTATTTTGTGGCGGT‑GAATTTTTCCAGACAAATAAAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTGGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCtga                                 <  2:465254/150‑1 (MQ=255)                                                                                                                         gTGGTAGTTTGTGGCGGG‑GAAATTTTTCAGAAAAATAAAAAAAATGGA‑GGTGGCCTGATGAGCGAAAGTAATAAACAACAGGGAGTGAATAAGTTGAGAGAGATTGTCGCTAACTTTACCGCCACGATTTTTAACCGAATGCCtgatg                               >  2:437946/1‑148 (MQ=255)                                                                                                                                tttGTGGCGGT‑AAATTTTTCCAGACAAATAAAAAAACTGGA‑GTGCCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAATTTAACAGATATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                      <  2:836153/150‑1 (MQ=255)                                                                                                                                 ttGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACGGGA‑GTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                     <  1:250234/150‑1 (MQ=255)                                                                                                                                    tgGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                  >  1:300090/1‑150 (MQ=255)                                                                                                                                          t‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa            >  1:35998/1‑150 (MQ=255)                                                                                                                                              aTTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc          >  1:26291/1‑149 (MQ=255)                                                                                                                                               tttttCCAGAAAAATACAAAAACTGGA‑GTTGCCATGATGAGCAAAAGAAATAAGCAACAGGCAGTAAATAAGTTAACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg        <  2:28192/150‑1 (MQ=255)                                                                                                                                                 tttCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt      <  1:651461/150‑1 (MQ=255)                                                                                                                                                   tCCAGAAAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa    <  1:657163/150‑1 (MQ=255)                                                                                                                                                    ccAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa    >  2:142892/1‑149 (MQ=255)                                                                                                                                                    ccAGACAAATACAAAAACGGGA‑GTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAg  <  2:41154/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                        GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206289‑3206585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑