Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,568,512 | 0 | C | A | 39.3% | 98.4 / 19.7 | 61 | R3L (CGT→CTT) | mdtM | multidrug efflux system protein |
Reads supporting (aligned to +/- strand): ref base C (9/28); new base A (24/0); total (33/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.04e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCAACCACACTGCCTGTGAAC > NC_000913/4568366‑4568659 | cagGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTgg < 1:613452/151‑1 (MQ=255) ggCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATa < 2:971971/151‑1 (MQ=255) ccAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATaa > 1:425545/1‑150 (MQ=255) ccAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGAAAAAAAAAGAGGcgaaa > 2:809573/1‑146 (MQ=255) cAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATaaca < 2:517599/151‑1 (MQ=255) cAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGAGGCCTaa > 2:549127/1‑149 (MQ=255) aCTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGTCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACACCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATaacaacc < 2:392839/151‑3 (MQ=255) gACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAaacaac < 1:748263/151‑1 (MQ=255) aaaaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCgg < 1:804845/150‑1 (MQ=255) aaaaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGga < 2:334415/151‑1 (MQ=255) aCGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGAAAAAAAAAGTGGGATAACAAAAAACTCCTTGTCCGGAGAAccc > 2:645805/1‑151 (MQ=255) acaTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAAAAAAAAACTCCTTGTCCGGAGAAACCCAAAggg > 2:909541/1‑151 (MQ=255) aTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACAAGGCATAAAAAACAACTCCTTGTCCGGAGAACCCCAAAgggg > 2:460968/1‑150 (MQ=255) aTGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAAc < 2:387941/151‑1 (MQ=255) gATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACg < 1:810126/150‑1 (MQ=255) gATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGa < 2:318712/151‑1 (MQ=255) aTCCCAGGCTGGCTCAGACCCGCCGCCAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGCCGACAAAAACTGGCATAAAAAAAAACCCCCTTGCCGGAGCACCCCCACCGGGAAAAcca > 2:798768/1‑148 (MQ=255) aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGaa < 2:754428/151‑1 (MQ=255) aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAAAAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAAAGa > 1:655280/1‑150 (MQ=255) cAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACATGGGCTAACAAAAAACACCTTGTTCGGAGAACCCCAACGGGGAAAAAGaaa > 2:699432/1‑148 (MQ=255) cAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGCGGCCTAAAAAACAACCCCTTGTCCGGAGAACCCCAAAGGGGAAAACGaaa > 2:150443/1‑148 (MQ=255) aGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAAACAACAACCCCTTGTCCGGAGAACCCCAACGGGGAAAAAGAAAtt > 2:1006524/1‑149 (MQ=255) aGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGGGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGaaaat > 1:606510/1‑147 (MQ=255) gCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAAt < 1:655840/151‑1 (MQ=255) ggATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCCACGGGGGAAACGAAATTCAATa > 1:302194/1‑149 (MQ=255) cAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCg < 2:1792/151‑1 (MQ=255) aGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAACAGGCATAACAAAAAACCCCCTGTCCGGAGAACCCCAACGGGGGAAAAGAAAATCAAAACCCTCgg > 2:439605/1‑151 (MQ=255) gATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACCCCCTGTCCGGGGAACCCCAAAGGGGAAAACGAAATTCAATACCGTCg > 2:111494/1‑149 (MQ=255) tCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGAGGCATAAAAAACAACTCCTTGTCCGGAGAACACCAACGGGGGAAAAGAAAATCAAAACCGTCgg > 2:73771/1‑148 (MQ=255) tCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGAAAAAAAAAGCGGCATAAAAAAAAAACCCTTTTCCGGGGAAACCCAAAGGGGAAAAAGAAAATCAAAACAGGCggg > 2:168709/1‑148 (MQ=255) ccGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAAAAAACAAATCCTTGTCCCGAGAACCCCAACGGGGGAAAAGAAATTTAATACcggcgg > 2:348280/1‑147 (MQ=255) cGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACg < 2:512697/151‑1 (MQ=255) gACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGGAAAAGAAATTCAATACCGTCGGTAATGACg > 1:784355/1‑150 (MQ=255) cAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGGAATGACGaa > 1:96757/1‑150 (MQ=255) aGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAg < 2:214991/151‑1 (MQ=255) aGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAg < 1:933005/151‑1 (MQ=255) aGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAg < 1:914651/151‑1 (MQ=255) gATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAg < 1:850808/150‑1 (MQ=255) aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGc < 1:942075/151‑1 (MQ=255) cGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAAAAAACAACTCCCTGTCCGGAGAACCCCCAAGGGGGAAACGAAATTCAATACCGTCGGGAAAGACGAAAGGCAgg > 1:1002404/1‑151 (MQ=255) aaaGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATTACAAAAAACTCCTTGTGCCGGGAAACCCAACAGGGAAAAAGAAAATTAAAACACTCGGTACAGACGAAAAGGAGGGgaga > 2:965497/1‑150 (MQ=255) aaGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAg < 2:333342/151‑1 (MQ=255) tCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGCAAAAAAAACTGGCAGAACAAACAACTCCTTGTCCGGAGAACCCCCACCGGGGAAAAGAAATTAAATAACGTCGGGCATGGCGAAAGGCAGGGGAGaa > 2:1002240/1‑147 (MQ=255) aTACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGGGGCATAAAAAAAAACTCCCTTTCCGGGGAACCCCAACGGGGAAAACGAAATTCAAAACCGCCGGGAATGACGAAAAGCCGGGGAAAAGGGCa > 2:932175/1‑150 (MQ=255) aTACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAACAAAAAACTCCCTTTCCCGAGGACCCCCAAGGGGGAAACGAAATTTAATACCCCCGGGAATTAAGAAAAGCAGGTGAGAAggg > 1:626207/1‑148 (MQ=255) tACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGGAAACGAAATTCAATAACGTCGGGAATGAAGAAAGGCAGGGGAGAAGGGCaa > 1:118899/1‑150 (MQ=255) aCAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAAc < 1:321341/150‑1 (MQ=255) aaaaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACgag < 1:764153/151‑1 (MQ=255) aTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAACAAAAAACTCCTTGTCCGGAGAACCCCAAAGGGGAAAACCAAATTTAATACCGTCGGTAATGAAGGAAGGGAGGGGGGAAGGGGAACgagaat > 1:686198/1‑149 (MQ=255) gCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAGCTCCGTGTCCGGAGAACGCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTg < 2:13819/146‑1 (MQ=255) gCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAAt < 1:188848/151‑1 (MQ=255) gCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAACAAACAACTCCTTGTCCGGGGAACCCCAACGGGGGAAACGAAATTCAATACCGTCGGGAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCaaa > 1:194579/1‑150 (MQ=255) cATCGGGAAAAACAGCGTGGCGGCATGGCGGGTTAAAAAACGTGGCCTAAAAAAAAACTCCTTGTCCGGGGAAACCCAACGGGGGAAAAGAAAATCAATACCGTCGGGAATGACGAAAAGACGGGGGGAAGGGCAACGAGAATGCAAATg > 2:894359/1‑150 (MQ=255) cATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGt < 1:426903/151‑1 (MQ=255) cATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGGGGCAAAACAAACAACTCCTTGTCCGGAGAAACCCAAAGGGGAAAACGAAATTCAATAACGTCGGGAAAGACGAAAGGCAGGGGAGAAGGGCAAAGAGACCTCAAATg > 1:248053/1‑150 (MQ=255) gAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGaa < 1:563165/151‑1 (MQ=255) aaaaaCAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAACAAACAAATCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTTAAAAACGTCGGGAATGACGAAAGGAAGGGGAGAAGGGCAAAGAGACTGCAAAAGTAATGaa > 2:947862/1‑150 (MQ=255) aaaaCAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGCATAACAAAAAACTCCCTGTCCGGGGAACCCCCACCGGGGAAACGAAAATTCACAACGTCGGGAATGACAAAAAGCAGGGGAGAAGGGAAACCAGACTGCAAATGGAATGAAtt > 2:731721/1‑151 (MQ=255) aGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATg < 1:916590/151‑1 (MQ=255) cggcATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCaa < 1:348479/151‑1 (MQ=255) aaCGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCAACCACACTGCCTGTGAac > 1:19604/1‑150 (MQ=255) | CAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCAACCACACTGCCTGTGAAC > NC_000913/4568366‑4568659 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |