Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,001,918 | 0 | A | C | 38.8% | 146.0 / 13.7 | 84 | intergenic (‑129/+192) | fliA/fliC | RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin) |
Reads supporting (aligned to +/- strand): ref base A (41/8); new base C (0/31); total (41/43) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.30e-15 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC > NC_000913/2001769‑2002066 | aGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAAc < 1:1514555/151‑1 (MQ=255) gCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAAcc < 2:1026765/151‑1 (MQ=255) gCGGTATAGAGTCAATTCACTATAAACAGCCGTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCATGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTGCATGTAAAT‑TCCAG‑GCA‑GAAAAAAAc < 2:594663/150‑1 (MQ=255) cGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAAccc > 2:536926/1‑151 (MQ=255) agagTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCgg > 1:832121/1‑151 (MQ=255) agTGACTTCCCGATAAGCGGCCTTGTGTTATAGGGTTTTTCGGTATGTTTATCCGTTTCTGCAGGGTTTTTAAGCGGACGTTTAGTGGGTGAAAGGAGGGGTTATTTGGGGGTGACAGTTAATT‑TCCAG‑GAA‑AAAAAACACCCCGCCGGt < 2:768853/150‑1 (MQ=255) agTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTg > 1:96695/1‑151 (MQ=255) tGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGc > 1:1550449/1‑151 (MQ=255) gAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCg > 1:861267/1‑151 (MQ=255) atttCACAAAAAACAGCCCTGCGTTATATGGGTTATCGGCATGTTTACCCATTTCTGCAGGGTTTTTAATCGGGCGATTAGTGGGTGAAATGAGGGTTTATTTGGGGTTTACAGGTAAAT‑TCAAG‑GCA‑AAAAAAACCCCCGCCGGTGGc < 2:789162/147‑1 (MQ=255) ttCAGGATAAACAGCCCGGGGTTATAGGATTTTTCGGCATGATTATCCTTTTCTGCGGGGTTTTTAATCGGACGATTAGGGGGTAAAAGGAGGGTTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAACCCCCGCCGGTGGCggg < 1:1568890/150‑1 (MQ=255) ttCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCgggg > 1:1269024/1‑151 (MQ=255) ttCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCgggg > 2:1058796/1‑151 (MQ=255) gATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGc > 1:1338841/1‑150 (MQ=255) gATAAACAGCCCTGCGTTATAGGAGTTATCGGAATGATTATCCGTTTCTGCGGGGTTTTAAAGGGGACGATTGGGGGGTAAAAGGGGGGTTTATTGGGGGGTTACAGGAAAAT‑CCAAG‑GCA‑GAAAAAACCCCCGCCGGTGGCGGGGaa < 1:1469172/148‑1 (MQ=255) aaaCAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGt > 1:614267/1‑151 (MQ=255) cAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCGGTGTTTTTAATCGGACGATTAGGGGGTAAAATGAGGGGTTTTTTGGGGTTTACAGGTAATT‑TCCAG‑GCA‑AAAAAAACCCCCGCCGGTGGCGTGGAAGCACGtt < 2:789170/149‑1 (MQ=255) agcgatgagTTATATGAGTTATCGGCATGATTATTAGTTTCTGGAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTAAAGGTAAAT‑TCCAG‑GGA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTgat > 2:335391/9‑149 (MQ=255) ccTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGAc > 1:356080/1‑151 (MQ=255) tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACa > 1:581387/1‑150 (MQ=255) cGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt > 2:119428/1‑151 (MQ=255) ttgtatGATTTATGGGCATGATGTTCCTTTTGTGCAGGGTTTTTATTCGTTATTATAGGGATGGAATTGAGGGGTTATTGGGGGTTTAAGGATAATT‑CCCAG‑ACA‑GAAAAAAGCCCCGCCGGTGGCGGGGAAGCACGTTGCTg < 2:451040/140‑1 (MQ=255) ttattggtgTTATCGGAATGATTACCCGTTTCTGATGGGTTTTTAAGCGGACGATTGGTGGGTGAAAGGGGGGGTTTTTTGGGGGTTCAAGGAAAAT‑GCCGG‑GAA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaa < 2:1553490/139‑1 (MQ=255) tatGATTTCGCGGAATGATTTTCGGTTTCTGCAGGGTTTTAAATGGGACGATTAGTGGGTAAAATGAGGGTTTTTTTGGGGGTTACAGTTAAAT‑TCAAG‑GAA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCGCGTTGCTGACAAATTgc < 2:905790/149‑1 (MQ=255) atGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCt > 1:717437/1‑151 (MQ=255) ttgtggtaatatttATCCTTTTCGGCAGGTTTTTTAATCGGACTTGTGGGGGGTAAAATGAGGGTTTTTTGGGGGGTGAAAGTTACAT‑TCCAG‑AAA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTGAt < 2:392902/138‑1 (MQ=255) tATCTGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGt > 2:1574689/1‑151 (MQ=255) gCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCg > 2:994811/1‑151 (MQ=255) gATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg > 1:424360/1‑151 (MQ=255) ttATCCGTTTCTGCGGGTTTTTTAACCGGAGAATTAGTGGGTGAAAGGGGGGTTTATTTGGGGGTAACAGTAATTT‑TCCAG‑GCA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGag < 2:232145/148‑2 (MQ=255) tATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAACACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcgg > 2:658726/1‑151 (MQ=255) aTCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGAGCTTTATGTTGCCGGATgcgg > 2:1126260/1‑150 (MQ=255) ccGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGTTTATTTGGGGGTGACAGGTAAAT‑CCCGG‑GAA‑AAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTGTATGTTGCCGGATgcg < 2:72566/147‑1 (MQ=255) tttCTGCGGGGTTTTTAATCGGACGATTAGTGGGTGAAATGGGGGTTTATTTGGGGGTTACAGTAAAAT‑CCCGG‑GCA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa < 1:396936/150‑1 (MQ=255) cTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGc > 1:1572777/1‑151 (MQ=255) cAGGGTTTTAAATCGAACGATGGGTGGGTAAAATGAGGGGTTTTTTGGGGGTTAGAGTTAAAT‑CCCAG‑GCA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaaa < 2:1180065/145‑1 (MQ=255) aGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTCTTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCAtt > 2:1301062/1‑150 (MQ=255) aGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt > 1:885908/1‑150 (MQ=255) ggttttttAATCGGACGATTAGGGGGTAAAATGGGGGTTTATTGGGGGGTTACAGGAAAAT‑CCCAG‑GCA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTa < 1:36932/147‑1 (MQ=255) gggTTTTTAATCGGGCATTTGGTGGGTGAAAGGAGGGGTTTTTGGGGGGTTGAAGGAAATT‑TCCGG‑GAA‑AAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTGTATGTTGCCGGATGCGGCGTAAACGCCTTAt < 2:255684/151‑1 (MQ=255) gggTTTTCAATCGGACGGTTGGGGGGGAAAAGGGGGGGTTATTTGGGGGTAAGAGGAAATT‑TCCAG‑GCA‑GAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt < 2:828702/149‑1 (MQ=255) gggTTTTAAACCGACCGTTGAGTGGGGGAAAGGAGGGGTTATTTGGGGGTTAAGGGTAATT‑CCCAG‑GCA‑GAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt < 2:263294/149‑1 (MQ=255) ttttaAATCGGACGATTAGTGGGTAAAAGGAGGGTTTATTGGGGGGTTACAGATAAAT‑TCCAG‑GAA‑GAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc < 1:21114/149‑1 (MQ=255) ttttAATCGGACGTGTAGTGGGTAAAATGGGGGGTTATTTGGGGGTTACAGTTAAAT‑TCCAG‑GCA‑GAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg < 2:153283/150‑1 (MQ=39) ttttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCgg > 2:117024/1‑151 (MQ=255) tttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc > 2:1007437/1‑151 (MQ=255) ttAATCGGACGATTAGTGGGTAAAATGGGGGGTTATTTGGGGGTTAAAGGAAAAT‑CCCAG‑GCA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc < 2:949144/150‑1 (MQ=38) tAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt > 1:379533/1‑151 (MQ=255) aaTCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt > 1:1479165/1‑150 (MQ=255) aaTCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GAA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa < 1:201662/151‑1 (MQ=255) tCGGACGATTAGGGGGTAAAATGGGGGGTTTTTGGGGGGTTACAGGAAAAT‑CCCAG‑CCA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc < 1:595506/150‑1 (MQ=37) cGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGCAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa > 1:473932/1‑150 (MQ=255) ggATTAGTGGGTGAAA‑GAGGGGTTTTTTTGGGGGGTGAAGTTAAT‑TCCGGCGAA‑CAAAGATCCGCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTTCCGGATGCGTCGTAATCGCCTTATCCGGCCTACAAAAAt < 2:1633286/150‑1 (MQ=19) gATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtg > 1:933191/1‑151 (MQ=255) aTTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtg > 2:273131/1‑150 (MQ=255) ttAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgtg > 2:530228/1‑151 (MQ=255) tgaGTGGGTGAAGGGGGGGTTTATGTGGGGTTTACAGATAACT‑CCCAG‑CCA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAt < 1:204702/146‑1 (MQ=25) tAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc > 1:135743/1‑151 (MQ=255) aGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc > 2:34541/1‑150 (MQ=255) tGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaa > 1:1170167/1‑150 (MQ=255) gggTAAAAGGGGGGTTTATTGGGGGGTTACAGGAAAAT‑CCCAG‑GAA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaaa < 1:1059640/150‑1 (MQ=37) gggaaaaggggggtTTATTTGGGGGTTACAGGTAAAT‑CCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAtt < 1:232689/141‑1 (MQ=255) ggTGAAATGGGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GAA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAtt < 1:546258/151‑1 (MQ=255) ggTAAAAGGGGGGGTTTTTTGGGGGTTAAAGGAAAAT‑TCCAG‑GAA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAtt < 2:503764/151‑1 (MQ=37) tGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTc > 2:1040645/1‑150 (MQ=255) gacaTGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGAACGTTGCTGACACATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAACTGTTCAAATTCa > 2:741014/4‑150 (MQ=255) aaaGGAGGGTTTATTGGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCa < 1:1238308/149‑1 (MQ=255) aaTGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAAt > 1:834029/1‑150 (MQ=255) aaTGAGGAGTTATTGGGGGGTTA‑AAGTAAATGTCCAG‑CAA‑AAAAAATTCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGTATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa < 2:1025150/149‑1 (MQ=25) tGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaa > 1:214194/1‑150 (MQ=255) tGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaa > 2:1032524/1‑150 (MQ=255) gggtTTATTTGGGGGTTCAAGAAAAAT‑CCCAG‑GAA‑AAAAAACCCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTg < 2:1092463/151‑1 (MQ=25) gggTTTTTTGGGGGTTAAAGGTAAAT‑ACCAG‑GAA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGc < 2:1591631/151‑1 (MQ=37) ttGGGGGTTACGGGTAATT‑TCCAG‑CAA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa < 2:1337888/150‑1 (MQ=37) gggggTTGCAGGGAAGG‑GCGAG‑GAG‑GAAAAAAGCGCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGGCTTATCCGGCGTACAAAAATGTGCAAATGCAAGAAATTGCaggggaa < 2:767499/149‑7 (MQ=21) gggggTTACAGGT‑AAT‑TCCTG‑GGATGAAAAAATCCCCGCCGGTGGCGGGGAAGCACGTTCCTGACGATTCGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGAATACAAAAATGTGGAAATTAAATAAATTGCTATGCaa < 2:657449/149‑1 (MQ=25) ggggTTACAGGTAATT‑TCCAG‑GAA‑AAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACtt < 2:87866/151‑1 (MQ=255) aCAGGAAAAT‑TCCAG‑GCA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAg < 2:581134/149‑1 (MQ=255) ggTAAAT‑CCCGG‑GAA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg < 1:953453/147‑1 (MQ=255) aaaT‑CCCAG‑GCA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGa < 2:1066459/149‑1 (MQ=255) aT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGCTGCTGACAAATTCCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTCGTAGGCCTGATa > 2:1551141/1‑149 (MQ=255) aattcccagagaaaaaaaacCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg < 2:473912/131‑1 (MQ=37) cAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgc > 1:842116/1‑150 (MQ=255) g‑gCA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagc < 1:991268/151‑1 (MQ=255) ggaaaaaaaacaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgca < 2:918034/144‑1 (MQ=255) gacaaaaaaaacCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcag < 1:1008249/145‑1 (MQ=255) gCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagcg > 1:743364/1‑151 (MQ=255) aaaaaaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAgcgcagcgca > 2:1412993/1‑149 (MQ=255) aaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGCTGCGGCGTAAACTCCTTATCCAGCCTACAAAAATGTGCAACTTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGc > 2:1349797/1‑150 (MQ=255) aaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGc > 2:1454611/1‑150 (MQ=255) aaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGAATCAgg > 2:762304/1‑149 (MQ=255) | AGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAT‑TCCAG‑GCA‑GAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC > NC_000913/2001769‑2002066 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |