Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,332,3090AC38.1% 136.2 / 35.1 84L322V (TTA→GTA) basSsensory histidine kinase in two‑component regulatory system with BasR
Reads supporting (aligned to +/- strand):  ref base A (41/11);  new base C (0/32);  total (41/43)
Fisher's exact test for biased strand distribution p-value = 3.91e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGC  >  NC_000913/4332160‑4332455
                                                                                                                                                        |                                                                                                                                                     
gCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTaa                                                                                                                                                       <  1:549403/150‑1 (MQ=255)
    gtggtCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAAcccc                                                                                                                                                  >  1:769370/1‑150 (MQ=255)
     tggtCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCa                                                                                                                                                 <  2:1409852/150‑1 (MQ=255)
      ggtCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAg                                                                                                                                                <  1:806863/150‑1 (MQ=255)
      ggtCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGa                                                                                                                                               <  1:1637276/151‑1 (MQ=255)
       gtCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGAc                                                                                                                                              >  1:1587649/1‑151 (MQ=255)
       gtCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGAc                                                                                                                                              <  1:326344/151‑1 (MQ=255)
          agcagcTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCaa                                                                                                                                          <  2:440214/151‑1 (MQ=255)
               ctttctttATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCg                                                                                                                                    >  1:1628665/1‑151 (MQ=255)
                 ttctttATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCg                                                                                                                                    <  1:1077861/149‑1 (MQ=255)
                  tctttATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGcc                                                                                                                                  >  2:1076965/1‑150 (MQ=255)
                   ctttATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCAt                                                                                                                                >  1:319085/1‑151 (MQ=255)
                    tttATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCAt                                                                                                                                >  1:1494525/1‑150 (MQ=255)
                       atatCTGGTTTGCCACGGACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGAGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAAc                                                                                                                             >  2:1122958/1‑150 (MQ=255)
                        tatCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACgg                                                                                                                           >  1:256936/1‑151 (MQ=255)
                            tGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGAGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGt                                                                                                                       >  2:654428/1‑151 (MQ=255)
                                    ccctttctgttttttcttCAGCCGTCCCCGGCCCCGCGGGCGGGATTTCTTTTCCCGTTTTTGCAGAAAAACCTCCCCGTGATCCATTTGTGTAATGCGGCTAACAATACTTACCCCCACCCCAATCCCGCCATAACGGCTGTCCATACGt                                                                                                               <  1:1407041/139‑1 (MQ=255)
                                    cccttccTGTTCTTTCTTCGGCCGACCCCCGGCCCGGGTGCGGAATTTCTCTTCCTGTTTTTGCAAAAAAACCCCCCCGTGATACAACTGTATAATGGTGCTGAAAATACCAACCCCCAGCCCATCCCCGCCATAACGGCTGTCCATACGt                                                                                                               <  2:568372/145‑1 (MQ=255)
                                    cACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGt                                                                                                               >  1:283183/1‑151 (MQ=255)
                                       gTACTGTTTTTTCTCCAGCCGCCCCCAGCCCCGGGTGCGGGAGGTCTCTTGCGGTTTTGGAAGAAAAACCCCCCCGTGTTCAATCTGTGTAATGCGGCTGACAATCCTACCCCCAACCCCAATCCCGCCATAACGGCTGTCCATACGTAc                                                                                                             <  1:1212232/150‑1 (MQ=255)
                                         aCTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACaa                                                                                                           >  1:181943/1‑150 (MQ=255)
                                          cTGATCTTTCTTCAGCCGGCCCCAGTCCCGCGTGCCGGAATTCTTTTGCCGGTTTGGCAGAAAAAACTTCCCGAGATGCAACTGTGTAAGGCGGCTGACAATACTTAACCCCAGACCAATCCTGCCATAACGGCTGTCCATACGTACaaa                                                                                                          <  1:1228635/150‑1 (MQ=255)
                                           tGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACg                                                                                                        >  1:635588/1‑151 (MQ=255)
                                           tGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACg                                                                                                        >  1:1210853/1‑151 (MQ=255)
                                            gttctttcttCAGCCGCCCCCGGCCCCGCGGGGCGAAATTCTCTGGCCGTTTTGGAAGAAAAACCTCCCCGTGATGAAATTGTTAAATGCGGCTAAAAATCCTAACCCCCAGCCCAACCCCGCCATAACGGCTGTCCATACGTACa                                                                                                            <  1:425571/144‑1 (MQ=255)
                                              tttttctcCACCCGTCCCCGGCCCCGCGGGCGGGAATTCTCTGGCGGTTTTGGCAGAAAAACCTCCCCGTGATCAAACTGTTTAATGCGGCTAAAAATACTAACCCCAAGCCAACCCCCGCCATAACGGCTGTCCATACGTACAAAc                                                                                                         <  1:459524/145‑1 (MQ=255)
                                              ttttctccccgCCGTCCCCGGCCCCGGGGGCGGGGTGTCTAGTGCCGTTTTGGAAAGAAACCCGGCCGGTGGTGCAACTGGGAAGGGCGGCGAACAATCCTAACCCCCAAACCACCCCCGCCATAACGGCTGTCCATACGTACa                                                                                                            <  2:536142/134‑1 (MQ=255)
                                                tttcttCCGCCGTCCCCGGCCCCGCGTGCCGAAATTCTTTTGCGGTTTTGGAAGAAAAACCGGCCCGTGATCAAACTGTTTAATGCGGCTAAAAATCCTAACCCCCACCCCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCttt                                                                                                    <  1:534923/150‑1 (MQ=255)
                                                tttctccccccGTCCCCGGCCCCGCGGGCGGGATGTCTTTTGCCGTTTTTGCAGAAAAACCTGCCCGTGATGCAATTGTGTAAGGCGGCTAACAATACTTACCCCCAGCCCAACCCCGCCATAACGGCTGTCCATACGTACAAACg                                                                                                        <  1:2474/137‑1 (MQ=255)
                                                tttctcCACCCGCCCCCAGCCCCGCGCGCCGAATGTCTCTGGCGGTTTTGGAAGAAAAACCGCCCCGTGATGCACTTGTGAAAGGCGGCTAAAAATCCTAACCCCCAGCCCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCttt                                                                                                    <  1:990306/150‑1 (MQ=255)
                                                   cttcAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTc                                                                                                >  1:150780/1‑151 (MQ=255)
                                                   ctccacccGCACCCGCGCCCGCGTGCCGGATGTCTCTTGCCGTTTTAGAAGAAAAACCCCCCCGTGAGCCAACTGTGTAATGCGGCTCACAATCCTAAACCCAACACCACCCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTa                                                                                                   <  2:213164/142‑1 (MQ=255)
                                                    ttcAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTc                                                                                                >  1:829154/1‑150 (MQ=255)
                                                    ttcAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTc                                                                                                >  2:539122/1‑150 (MQ=255)
                                                    ttcAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTc                                                                                                >  1:1467328/1‑150 (MQ=255)
                                                    tccAGCCGTCCCCGGCCCCGCGGGCGGGAATTCTCTTGCGGTTTTTGAAGAAAAAACTCCCCGTGATACAATTGTGTAATGCGGCTGACAATATTAACCCCCACACCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTAc                                                                                                  <  1:1193578/146‑1 (MQ=255)
                                                      cggccgcccccgcgCCCGCGTGCCGGAGGTCTCTTGCCGGTTGACCAGAAAAACCTGCCGGGGCTACATTTTTGTACGGCGGCTCACAAAACCAACCCCCAGTACAATCCCGCCATAACGGCTGTCCATACGTACAAACGCt                                                                                                      <  2:634291/129‑1 (MQ=255)
                                                       aGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACt                                                                                            >  1:921899/1‑151 (MQ=255)
                                                         cctcccccggccccGCGGCCGGAAGTTCTTTGGCGGTTTTGGAAAGAAAACCCCCCCGTAATCCAATTGTGTAGTGGGCCTGAAAACACTAACCCCCAGCCCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCa                                                                                               <  1:1244719/135‑1 (MQ=255)
                                                         ccgccccaaGGCCCGCGTGCGGGAACTCTCTTGCGGTTTTTGGAGAAAAACCTCCCCGTTATCAATCTTTTAAATGCGCCAAAAAATCCTACCCCCCACCCCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACt                                                                                            <  1:1504416/141‑1 (MQ=255)
                                                         ccGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTcc                                                                                          >  2:1492283/1‑151 (MQ=255)
                                                          cGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTccc                                                                                         >  1:798968/1‑151 (MQ=255)
                                                              cccggccgcgcgGGCGGAAAGTCTTTGGCTGTTTTTGAAAAAAAAACGGCCTGTAATACACGTTTGAAATGCGGCTAAAAATACTAACCCCCAAACAAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCg                                                                                       <  1:360204/141‑1 (MQ=255)
                                                              cccggccccGCGTGCCGGAATTTTTTTGCGGTTTTGGAAAAAAAACCCCCCCGTGATGCAACTGTGTAAGGCGGCTAACAATCCTACCCCCCACCCCACCCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTc                                                                                                <  1:284963/134‑1 (MQ=255)
                                                                cgggCCCGCGTGCCGGAATTTTCTGGCTGGTTTTGAAAAAAACCCCGCCCGTGATGCATGTATGTAGCGCGGCACACAATACTAACCCCCAGCCCACCCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCAtt                                                                                   <  2:1626337/149‑1 (MQ=255)
                                                                  gccccGCGGCCCGGATTTCTTTTGCGGTTTTGGAAGAAAAACCCCCCCGTGAGCCAACTGTTAAATGGGGCTAACAAAACTAACCCCCAGACCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCAt                                                                                    <  1:764571/146‑1 (MQ=255)
                                                                   gCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTAc                                                                                >  1:859421/1‑151 (MQ=255)
                                                                             cgggacttccctggcGGTTTTGGCAGAAAAACCCCCCCGGGAGCCATTTGTGTATTGCCGCGAACAATACTAACCCCCACACAAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCtcat                                                                          <  1:1500440/134‑1 (MQ=255)
                                                                              ggAGGTCTCTGGCTGTTTTGGAAGAAAAACCTGCCCGTAATGCATTTTTGTAATGCGGCTAACAATTCTAACCCCCACCCAAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCaa                                                                       <  1:964632/149‑1 (MQ=255)
                                                                              gaaaGTCTCTTGCGGTTTTGGAAGAAAAAACCCCCCGTGATGCATTTGTGTAAGGCGGCAAAAAATACTCACCCCCAAACCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCtcatca                                                                        <  1:521483/146‑1 (MQ=255)
                                                                                     tctTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTc                                                              >  1:1508602/1‑151 (MQ=255)
                                                                                       ttGCCTTTTTCGCAGAAAAAACTGCCCGTAATAATACTGTGTTGTGGGGCTGACATTACTAAACCCCAGCACAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATAc                                                                    <  2:955304/143‑1 (MQ=255)
                                                                                         ggcGGTTTTGAAGAAAACCCTCCCCTGGATGCAATTGTGTAAGGCGTCCAAAATTACTAACCCCCACCCAAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTc                                                              <  2:1504988/145‑1 (MQ=255)
                                                                                           cGGTTTTGGAAGAAAAACCTCCCCGTGATCCAATTTTTTAAGGCGGCTAAAAATCCTCACCCCCACACCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCt                                                                  <  1:1075852/142‑1 (MQ=255)
                                                                                            ggctttGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCAt                                                        >  2:111728/4‑150 (MQ=255)
                                                                                             gTTTTGGAAGAAAAACCTCCCCGGGAGCAAACTGTGTAGGGCGGCTAAAAATACTAACCCCCAACCAAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCAt                                                        <  1:635790/150‑1 (MQ=255)
                                                                                               tttggaagaaaaacCTCCCCGGGATGCAACTGTGTAAGGCGGCTAAAAATACTAACCCCCAAACAAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTg                                                                 <  1:1064096/130‑1 (MQ=255)
                                                                                               tttGCAGAAAAACCTCCCGGTGATCAATCTGTTTAAGGCGCCTAAAAATACTCACCCCCAGCCCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCtt                                                     <  1:651046/150‑1 (MQ=255)
                                                                                                       aaaaaaCGCCCGGTGATGCACCTGTGTAATGGGGCTAAAAATCCTCACCCCAAGACAAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACt                                                <  1:943676/147‑1 (MQ=255)
                                                                                                        caaaaCTGCCCGCGCTGCAACTGCGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCCTAACGCCTGTCCATACGCACAAACGCTTTCCTCACCTCCCCGCATTTCCTCTCATCCATCCCTGGTCCTTCATCTTCAACTGcc                                             >  2:676281/2‑150 (MQ=255)
                                                                                                         aaaaCTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCAt                                           >  1:320835/1‑151 (MQ=255)
                                                                                                               tGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGAcc                                       >  1:1429311/1‑150 (MQ=255)
                                                                                                               tGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATAAGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGAcc                                       >  2:1142127/1‑150 (MQ=255)
                                                                                                                  ctggTGATCGAACTGTGAAATGCGGCTAACAATACATACCCCCACACCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGa                                         <  2:1364427/143‑1 (MQ=255)
                                                                                                                  ccggTGATGAAATTGTGAAACGCGGCAAAAAATTCTAACCCCCACCCCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCt                                    <  1:972294/148‑1 (MQ=255)
                                                                                                                  ccctatagccacgtgttTATTGCGCCTCACAAACTTAACCCCCACACCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGAcc                                       <  1:110756/135‑1 (MQ=255)
                                                                                                                  cccGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCt                                    >  1:792854/1‑151 (MQ=255)
                                                                                                                  accGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGc                                     >  2:447136/2‑150 (MQ=255)
                                                                                                                   ccGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTc                                   >  2:410972/1‑151 (MQ=255)
                                                                                                                        acccAACTGTGTAAGGGGGCAGACAATACTAACCCCCACCCCACCCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCa                              <  1:17474/148‑1 (MQ=255)
                                                                                                                          gCAACTGTGTAATGCGGCTGCCAATACTTAACCCCAGACCAATCCCGCCATAACAGCTGTCCATAAGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCCATACCTGGTCCTTCATCTTCAACTGCCACGACCGCTCCGTCa                              >  2:1378302/1‑149 (MQ=255)
                                                                                                                           cAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCAtct                           >  2:826027/1‑151 (MQ=255)
                                                                                                                            aaCTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCAtctt                          >  2:1480662/1‑151 (MQ=255)
                                                                                                                                    actGCGCCTAAAAATCCTCACCCCCACACAATCCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAg                   <  1:343661/148‑1 (MQ=255)
                                                                                                                                    aaTGCGGCTGAAAATACTTAACCCCAGAACAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGc                  >  2:75391/1‑151 (MQ=255)
                                                                                                                                     aTGCGGCTGAAAATACTCCCCCCCACACCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCAt                             <  2:858653/139‑1 (MQ=255)
                                                                                                                                      tGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGCCGGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAACACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCtt                >  2:1172444/1‑151 (MQ=255)
                                                                                                                                        cGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTa               >  1:1236855/1‑150 (MQ=255)
                                                                                                                                         ggcaaaCAATACTCACCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTa               <  1:1400962/144‑1 (MQ=255)
                                                                                                                                         gccTGAAAATACTCACCCCCACCCCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCtt                <  1:1471034/146‑1 (MQ=255)
                                                                                                                                            tGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCAt          >  1:970419/1‑151 (MQ=255)
                                                                                                                                              aCAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATa         >  1:1286895/1‑150 (MQ=255)
                                                                                                                                               cagccctaaaCCCCAACCCAAATCCCGCCATACCGGCTGTCCATACGTACAAACGCTTTCCTCAACTCCCCGCATTTACTCGCATCAATGCCTGGTCCTTCAGCTTCAACTGCCATGTCCGCTCCGTGATCTTCTTGCAGCTTAATCAt          <  2:382952/141‑1 (MQ=255)
                                                                                                                                                   aCTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTtg   >  2:567812/1‑151 (MQ=255)
                                                                                                                                                     ttAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCCTCTTCTTGCAGCTTAATCATAATGTtgc  >  2:1603160/1‑150 (MQ=255)
                                                                                                                                                     ttAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTtg   >  2:166729/1‑149 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
GCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGC  >  NC_000913/4332160‑4332455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: