Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5760TG44.0% 137.4 / 36.3 100V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base T (7/49);  new base G (43/1);  total (50/50)
Fisher's exact test for biased strand distribution p-value = 2.03e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

AACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACA  >  NC_000913/599427‑599722
                                                                                                                                                     |                                                                                                                                                   
aaCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggtg                                                                                                                                                    <  1:135212/151‑1 (MQ=255)
 aCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGgggggg                                                                                                                                                   >  2:1486654/1‑151 (MQ=255)
   gCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggtggta                                                                                                                                                 <  2:173815/151‑2 (MQ=255)
    cTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGACTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGggggggggggggg                                                                                                                                                >  2:208003/1‑148 (MQ=255)
     tCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGgggggggg                                                                                                                                                 >  2:1292231/1‑147 (MQ=255)
     tCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGa                                                                                                                                                >  1:946516/1‑150 (MQ=255)
       gacgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATca                                                                                                                                             <  2:111541/151‑2 (MQ=255)
          gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGc                                                                                                                                          <  1:1357373/151‑1 (MQ=255)
          gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTcc                                                                                                                                             >  1:524463/1‑148 (MQ=255)
          gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGc                                                                                                                                          >  1:1575045/1‑151 (MQ=255)
              ttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATc                                                                                                                                      >  1:1165960/1‑151 (MQ=255)
                aaTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCg                                                                                                                                    <  2:961926/151‑1 (MQ=255)
                   cacaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCgg                                                                                                                                   >  1:561256/1‑149 (MQ=255)
                     caTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCaa                                                                                                                                >  1:625011/1‑150 (MQ=255)
                      aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCaaaa                                                                                                                              <  2:284340/151‑1 (MQ=255)
                      aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCaaa                                                                                                                               >  1:251422/1‑150 (MQ=255)
                           tttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGc                                                                                                                          <  2:227879/150‑1 (MQ=255)
                           tttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGcc                                                                                                                         >  1:559506/1‑151 (MQ=255)
                             tAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCCGCGGTTCGGCAAAAAAGGCCg                                                                                                                       >  1:1053680/1‑151 (MQ=255)
                             tAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATTCTGCGATCCGGCAAAAAAGccc                                                                                                                        >  1:1636812/1‑150 (MQ=255)
                             tAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGAGTCTGCGACCCGGCAAAAAAGcc                                                                                                                         >  2:1190582/1‑149 (MQ=255)
                             tAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGAGCCTGCGAACCGGCACAAACGccc                                                                                                                        >  2:440823/1‑150 (MQ=255)
                              aaaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCCGGGGGGGGATGCTGCGGTCCGGCGAAAACGCCCg                                                                                                                       >  2:684317/1‑150 (MQ=255)
                               aaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGGGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAATCGCCCga                                                                                                                      <  2:952025/150‑1 (MQ=255)
                               aaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTCCGGGCCCGCGAAAAAGccc                                                                                                                        >  2:464708/1‑148 (MQ=255)
                                   ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCCGCGGACCGGCCAAAAAACCCGAGAAg                                                                                                                  >  2:777908/1‑150 (MQ=255)
                                   ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGAGaa                                                                                                                   >  1:740059/1‑149 (MQ=255)
                                      gTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGAt                                                                                                              <  2:1408058/151‑1 (MQ=255)
                                             tGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGGGATCgccgcc                                                                                                       >  1:1379929/1‑151 (MQ=255)
                                              ggCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCgccgcc                                                                                                       <  2:906823/150‑1 (MQ=255)
                                              ggCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGACGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCgccgcc                                                                                                       <  1:957885/150‑1 (MQ=255)
                                                   gCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAg                                                                                                 <  1:1183989/151‑1 (MQ=255)
                                                   gCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTg                                                                                                    >  1:1277419/1‑148 (MQ=255)
                                                     ccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGCGAAgg                                                                                                >  1:977755/1‑150 (MQ=255)
                                                     ccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGa                                                                                               <  1:1242666/151‑1 (MQ=255)
                                                      cGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCCTTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGTGGGGATACTGCGCTCCCGCAAAAACGCCCGAGGAGTGGTCGCCGCCGGGAAgg                                                                                                >  2:1226669/1‑149 (MQ=255)
                                                      cGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAgg                                                                                                <  1:1080835/149‑1 (MQ=255)
                                                       gTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGAAgg                                                                                                >  1:245491/1‑148 (MQ=255)
                                                        tttATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGACGGCGAAGTGGCGGGCGGGGGGGGGGGCCTGCGATACGGCCAAAACGCACGAGAAGAGATAGCCGCCGTGGAGGCCa                                                                                             >  2:1139227/1‑150 (MQ=255)
                                                         ttATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCCAAAAAGCCCGGGGAGGGATGGCCGCCGGGAAggggaa                                                                                            >  2:1229779/1‑146 (MQ=255)
                                                           aTCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGGAGGACaa‑a                                                                                          >  1:1009586/1‑149 (MQ=255)
                                                             cTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGAACCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGCGAAGGACAA‑AATgg                                                                                      >  1:123533/1‑151 (MQ=255)
                                                               gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTgg                                                                                      <  2:1266280/149‑1 (MQ=255)
                                                               gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGa                                                                                     <  1:464902/150‑1 (MQ=255)
                                                                 gcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAAc                                                                                  <  1:48244/151‑1 (MQ=255)
                                                                 gcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGCACGGCAAAAAACCCCGAGAAGGGATCGCCGCGGCGAAGGAGAA‑ACGGGgaa                                                                                   >  2:272137/1‑150 (MQ=255)
                                                                   gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGc                                                                                <  1:574550/151‑1 (MQ=255)
                                                                   gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGc                                                                                <  1:22709/151‑1 (MQ=255)
                                                                    aTGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCt                                                                               <  1:1313032/151‑1 (MQ=255)
                                                                     tGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGTGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGAACGCCGCCGGGAAGGACAA‑ACTGGAAACGc                                                                                >  2:400302/1‑149 (MQ=255)
                                                                       ttGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCCGCGCTCCCGCAAAAACGCACGAGGGGGGAACGCCCCCGGGGAGGACAA‑ACTTGaaa                                                                                   >  2:1046732/1‑144 (MQ=255)
                                                                         gCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGACCTAAACGGGGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGaa                                                                            <  2:880829/149‑1 (MQ=255)
                                                                          cGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCCTCGATCCGGCAAAAACGACCGAGAAGTGATCGCCGCCGGGAAGGGCAA‑ACTGGAAAAGCTGaaa                                                                           >  2:423556/1‑149 (MQ=255)
                                                                           gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAagt                                                                        <  1:1564634/151‑1 (MQ=255)
                                                                             aGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAagtag                                                                      <  1:1129957/151‑1 (MQ=255)
                                                                               gTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAagtag                                                                      >  1:620091/1‑149 (MQ=255)
                                                                                tCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGGGGCGGGCCGGGGGGGGGTCGCGCGATTCCGCCAAAACGCCCTGGGTGTGATGGCCGCCCTGGAGGGCAA‑ACTGGAAACg                                                                                 >  2:473886/1‑137 (MQ=255)
                                                                                 ccAGATTGGCCCGGAGAGGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTg                                                                  <  2:960604/151‑1 (MQ=255)
                                                                                   aGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTACTGCGGTCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGGGAAGGACAA‑AATGGGAACCCTGAAAAGAAGGTTGcc                                                                >  2:478002/1‑151 (MQ=255)
                                                                                   aGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGGCCTGCGATCCCGCCAAAACAACCGAGGAGGGAGCGCCCCCGGGAGGGACAA‑ACTTGAAACGGCGAAAAATAGTATGcc                                                                >  2:691720/1‑151 (MQ=255)
                                                                                   aGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGGGAAGGACAA‑ACTGGAAAAGCTGAAAAGAAGTCTGcc                                                                >  2:575910/1‑151 (MQ=255)
                                                                                   aGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGGGAAGTGATCGCCGCCGGGAAGGACAA‑AATGGAAACGCTGAAAAGTAGTCTGcc                                                                >  1:1129599/1‑151 (MQ=255)
                                                                                    gATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCGGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCg                                                               <  1:873287/151‑1 (MQ=255)
                                                                                         gCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGGTCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGaa                                                            <  2:1593085/149‑1 (MQ=255)
                                                                                         gCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCCGCGATCCGGCAAAAAAGCCCGAGGAGGGATCGCCGCCGTGGAGGGCAA‑ACAGGGAAAGCTGGAAAGAAATTTGcccg                                                              >  2:915837/1‑145 (MQ=255)
                                                                                              gagaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTgg                                                     <  1:1250699/151‑1 (MQ=255)
                                                                                               agaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGACAA‑ACTGGAAAAGCTGAAAAGTAGTCTGCCGGAAGGCGTgg                                                     >  1:1302213/1‑150 (MQ=255)
                                                                                                 aTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGgag                                                   <  1:1287968/150‑1 (MQ=255)
                                                                                                   gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGTGGTCCACCGATCCGGCAAAAAAGCCCGAGAAGGGGTCGGCACCGTGAAGGACAA‑CCTGGAAACGCTGAAAAAGAAGCTGCCGGGAggggggg                                                     >  2:400871/1‑141 (MQ=255)
                                                                                                   gagcCGGGGCATTGCCGAACTAAACGGCGCAGGAGAAGTGGCGGGGGGGGGGGGGGCCCTTCCAAACGGCAAAAAAGCCCGGGGAGGGGTCGCCGGCGCGGAGGGCAACACAGG‑AACCCCGAAAAGAAGTCCTCCGGCAGGGGGGgag                                                   >  2:1281113/3‑148 (MQ=255)
                                                                                                       cGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTa                                            <  1:268396/151‑1 (MQ=255)
                                                                                                       cGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATTCTGCGCTACGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGTGAAGGACAA‑ACTGGAAAAGCCGAAAAGTAGGCCGCCGGAAGGCggg                                                      >  2:1103391/1‑139 (MQ=255)
                                                                                                        ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTac                                           <  1:1577090/151‑1 (MQ=255)
                                                                                                        ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTac                                           <  2:936823/151‑1 (MQ=255)
                                                                                                        ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGAAAGGCGTGGAGATAGTTaa                                           <  2:1629062/151‑2 (MQ=255)
                                                                                                        ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCGGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGtt                                             <  2:369559/149‑1 (MQ=255)
                                                                                                         gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTaca                                          <  1:521606/151‑1 (MQ=255)
                                                                                                               tGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATa                                     <  2:884961/150‑1 (MQ=255)
                                                                                                               tGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATa                                     <  2:1061513/150‑1 (MQ=255)
                                                                                                                gCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACg                                   <  1:1199541/151‑1 (MQ=255)
                                                                                                                gCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGGCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGAAGGACAA‑ACTGGAAACGCTGAAAAGGAGTCTGCCGGGAGGGGTGGAGATAGTTacaacaaa                                     >  2:745062/1‑147 (MQ=255)
                                                                                                                   gAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAAAGCTGAAAAGAAGTCTGCCGGGAGGCGGGGAGATAGTTACAACAt                                      >  1:503175/1‑145 (MQ=255)
                                                                                                                       tAAACGGCGAAGGCGAAGTGGCGGGGGGGGTGGTGTTCCTGCGATACGGCCAAAACGCCCGAGAAGGGGTCGCCCCCGGGAAGGAAAA‑ACTGCAAACCGCGAAAAGAAGTCTGGCCGAAGGCGTGGAGCTAGTTAAAAAATAccgac                                >  2:693755/1‑143 (MQ=255)
                                                                                                                          aCGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagcca                         <  2:38789/151‑1 (MQ=255)
                                                                                                                           cGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagccag                        <  1:401966/151‑1 (MQ=255)
                                                                                                                             gcgaaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCt                      <  2:725904/151‑1 (MQ=255)
                                                                                                                              cgaaggcgaagTGGCGGGCGGGGGGGTGATCCTGTGGTCCGGCAAAAACGCCCGAGAAGGGGTCGCCGCCGTGGAGGACCA‑ACTGGGAACGCTGAAAAGTAGACTGCCGGGAAGGGTGGAGATAGTTACAACAAACGATCGCAGCCAGCt                      >  2:110740/1‑150 (MQ=255)
                                                                                                                               gaaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCa                    <  2:251347/151‑1 (MQ=255)
                                                                                                                                aaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCAt                   <  1:1404146/151‑1 (MQ=255)
                                                                                                                                aaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGAGCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCa                    <  2:551748/150‑1 (MQ=255)
                                                                                                                                   gggggggggggggcggtGGGGTGATGGTGCGATCGGGGAAAACCGCCCGAGGGGTGGTCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTGGTCTGCCGGAAGGCGTTGGGATAGTTAAAACATACGATGGCGGCTGGCTCa                    <  2:1251194/137‑1 (MQ=255)
                                                                                                                                       agTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGAAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgc            <  2:869977/151‑1 (MQ=255)
                                                                                                                                         tGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgcg           >  2:902827/1‑150 (MQ=255)
                                                                                                                                         tGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCgcgc          >  1:1548341/1‑151 (MQ=255)
                                                                                                                                           gcgggcggGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGcc         >  1:230126/1‑150 (MQ=255)
                                                                                                                                             gtgcggcgTGCTGATCCTGCGCTCCGGCACAAACACCCGAGAAGTGATCGCCGGCGTGAAAGCAAA‑ACCGGACACGCTGGAAAGTAGGCTGCCGGGCGGCGGGAAGCTAGTTACAACATACGCTCCCAGCCAGCTCATTGACCGCGcc         >  2:870013/3‑148 (MQ=255)
                                                                                                                                              ggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCg     <  1:1601764/151‑1 (MQ=255)
                                                                                                                                              ggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCg     <  2:1499547/151‑1 (MQ=255)
                                                                                                                                                cgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGAc   <  1:1293176/151‑1 (MQ=255)
                                                                                                                                                 ggggTGGTGATCATGCGAGCCGGCAAAAACGCCAGAGAGATGATCGCCGCCTTAAAGGACAG‑ACTGGAAAAGATGAGAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATAAGATCGCAGACAGCTCATTGACCGCGCCATCGAcg  <  2:881518/151‑2 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAA‑ACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACA  >  NC_000913/599427‑599722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑