Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,646	0	T	G	46.4%	77.8 / 12.4	56	V316G (GTA→GGA)	yeiI	putative kinase
Reads supporting (aligned to +/- strand):  ref base T (8/22);  new base G (26/0);  total (34/22)
Fisher's exact test for biased strand distribution p-value = 3.66e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTG  >  NC_000913/2252496‑2252796                                                                                                                                                       |                                                                                                                                                          atCAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGt                                                                                                                                                           <  2:104085/151‑1 (MQ=255)   cAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGta                                                                                                                                                          <  2:509270/150‑1 (MQ=255)   cAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGACCTGGta                                                                                                                                                          <  1:530944/150‑1 (MQ=255)      cAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGtgttt                                                                                                                                                       >  1:73343/1‑146 (MQ=255)            gtTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑ttt                                                                                                                                                <  1:645014/150‑1 (MQ=255)                    aCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGa                                                                                                                                       <  1:530880/151‑1 (MQ=255)                    aCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATATC‑tttttttta                                                                                                                                          >  2:1008110/1‑145 (MQ=255)                       gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATAGA‑tttttttttgg                                                                                                                                        >  1:715842/1‑142 (MQ=255)                        tttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGG‑TTTTTTTTAGGAt                                                                                                                                      >  2:146008/1‑148 (MQ=255)                         ttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAGG‑TTTTTTTAAg                                                                                                                                         >  2:672584/1‑144 (MQ=255)                          tgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATTTAGG‑TTTTTTTTAGGATaaa                                                                                                                                   >  1:138830/1‑147 (MQ=255)                           gtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑Gtt                                                                                                                               <  1:301260/151‑1 (MQ=255)                            tttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAGT‑TTTTTTTTAGGATAAA‑Gttt                                                                                                                              >  2:278478/1‑151 (MQ=255)                             ttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑Gttt                                                                                                                              <  1:337388/150‑1 (MQ=255)                              tATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATTTAACTTTTTTTGAAggtaaaagt                                                                                                                                 >  2:612238/1‑141 (MQ=255)                                  tGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCGTGGGTTATAAC‑TTTTTTTTAGGGTACaaatt                                                                                                                               >  2:163539/1‑141 (MQ=255)                                   gCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATTGTTTTATGGCGGGCCTGGGGTATAAC‑TTTTTTTTAGGATACA‑Gttt                                                                                                                              >  2:511888/1‑144 (MQ=255)                                    cccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACa                                                                                                                      <  2:644508/151‑1 (MQ=255)                                         tGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGTTATAAG‑TTTTTTTTAGGAGAAA‑GATTTCGCGGCAGCGcc                                                                                                                 >  1:830712/1‑151 (MQ=255)                                          gAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAAG‑TTTTTTTAAGGATAAA‑GGTTTCGCGGCAGCGccc                                                                                                                >  2:271766/1‑151 (MQ=255)                                                gTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTg                                                                                                          <  2:635543/151‑1 (MQ=255)                                                      tGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTa                                                                                                     <  2:1045814/150‑1 (MQ=255)                                                              aaaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGcgcg                                                                                            <  1:335197/151‑1 (MQ=255)                                                              aaaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAAC‑TTTTTTTGAGGGTACA‑GTTTTCCCGACAGCGCCCCtttt                                                                                                           >  2:563326/1‑136 (MQ=255)                                                               aaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCgg                                                                                           <  2:677422/151‑1 (MQ=255)                                                                 ggATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGGTATAGG‑TTTTTTTAAGGGTAAA‑GTTTTCGCGACAGCGCCCGGTTTTCGGTAGGCTGCgggg                                                                                           >  2:301986/1‑149 (MQ=255)                                                                     ggAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AAttt                                                                                    <  1:462365/151‑1 (MQ=255)                                                                      gagaACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTc                                                                                   <  1:424938/151‑1 (MQ=255)                                                                         aaCAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTcgcg                                                                                <  1:641172/151‑1 (MQ=255)                                                                         aaCAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAAC‑TTTTTTTAAGGATACA‑GTTTTCTCGACAGCGCCCCCTTTTCGGTTGCCTGCGCGGC‑CAttttt                                                                                  >  2:400068/1‑147 (MQ=255)                                                                             aTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGG‑TTTTTTTAAGGATAAA‑GTTTCCGCGACAGCGCCCGGTTTTCGGTAGGCTGCGCGGC‑AATTTTGCGCGcc                                                                            >  2:301038/1‑151 (MQ=255)                                                                             aTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAGC‑TTTCTTTTAGGGTAAA‑GTTTTCTCGCCCGCGCCCGGTTTTTGGGAGGCCGCGCGGC‑CATTTCTCGCGcc                                                                            >  1:268840/1‑151 (MQ=255)                                                                              tttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATATA‑TTTTTTTTAAGAGACA‑GATTTCGCCGCAACGCCCCCTTTTCGGGAGGCCGCGCGGCGAAtttgcccccc                                                                             >  2:704641/1‑143 (MQ=255)                                                                               ttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAGC‑TTTTTTTTAGGGGACA‑GTTTTCGCGACAGCGCCCGGTTTTCGGGGGCCTGCGCGGG‑AATTTTtcgcgc                                                                             >  1:532003/1‑148 (MQ=255)                                                                               ttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCa                                                                           <  1:271698/150‑1 (MQ=255)                                                                                tttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGGTATAAG‑TTTTTTTTAGGGAAAA‑GTTTTCCCGAACGCGCCCGGTTTGCGGTAGGCTGGGCGGC‑CATTTTGGGCGcc                                                                            >  2:681954/1‑148 (MQ=255)                                                                                  tGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAAC‑TTTCTTTTAGGATACA‑GGTTTCCCGACAACCCCCCCTTTTCGGGTGCCTGCGCCGC‑CATTTTTCGCGcccccgg                                                                       >  2:645441/1‑146 (MQ=255)                                                                                   gCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGTTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGc                                                                      <  1:442834/151‑1 (MQ=255)                                                                                   gCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGC‑TTTTTTTTAGGGAAAA‑GATTTCGCGGCAGCGCCCGTTTTTCGGGAGGCTGCGCGGC‑CAATTTGCGCGCCAGCgg                                                                       >  2:908133/1‑150 (MQ=255)                                                                                    cTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATAGC‑TTTTTTTTAGGATAAA‑GTTTTCGCGGCAGCGCCCCCTTTTCGGGAGGCCGCGCGGC‑CATTTTCCGCGCCAGCgg                                                                       >  1:382247/1‑149 (MQ=255)                                                                                    cTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGTATATAGC‑TTTTTTTTAGGGTAAA‑GTTTTCCCGACAGCGCCCCCTTTTCGGGAGCCCGCGCGGC‑GAATTTGCGCGCCCGCgg                                                                       >  2:567551/1‑149 (MQ=255)                                                                                       aCCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAAC‑TTTTTTTTAGGGTACA‑ATTTTCGCGCCCGCGCCCCTTTTTGGGGTGCCTTGGCGGC‑CAtttttcccgcccccgg                                                                       >  1:260730/1‑133 (MQ=255)                                                                                         cgcgCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTa                                                                <  2:979442/151‑1 (MQ=255)                                                                                         cgcgCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTa                                                                <  1:661169/151‑1 (MQ=255)                                                                                              cAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGAGATAGG‑TTTTTTGTAGGATAAA‑GATTTCCCCACAGCGGCCCCTTTTCTGGAGCCTGCGCCGC‑AAATTCTCGCGCCACCgggcgccttaa                                                              >  1:920680/1‑139 (MQ=255)                                                                                                  gcATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATTTAGA‑TTTTTTTTAGGATAAA‑GTTTTCCCGGCAGCGCCCCGTTTGCGGTAGCCTGCCCGGC‑CATTTTGCGCGCCCGCGGCCGCTTTAAAAaacc                                                        >  1:648829/1‑150 (MQ=255)                                                                                                           aGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCt                                              <  1:248122/151‑1 (MQ=255)                                                                                                              aGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGC‑TTTTTTTTAGGATAAA‑GTTTTCGCGACAGCGCCCCCTTTTCGGGAGCCCGGGCGGC‑AATTTTGCGCGCCCGCGGCCGCTTTAAAAACCCCCCCCCCTCTGcc                                           >  2:430969/1‑151 (MQ=255)                                                                                                               gACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAGC‑TTTTTTTTAGGGTACA‑GTTTTCGCGGCAGCGCCCGCTTTTTGGTAGCCCGCGCGGG‑CATTTTTCGCCCCCGCGGCAGCTTTAACAAACCTACCCCCTCTGcc                                           >  1:810869/1‑150 (MQ=255)                                                                                                                  aGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGG‑TTTTTTTGAGGATACA‑GTTTTCGCGGCAGCGCCCGGTTTTCGGGAGGCTGCGCGGC‑AATTTTTCGCGCCCGCGGCAGCCTAAAAAACCCCACCCCGTCTGcc                                           >  2:734139/1‑147 (MQ=255)                                                                                                                    ttttGTTGCTGACGTTGTTTTTAGGGCGGGCTTGGTATATAGC‑TTTCTTGAAGGATACA‑TTTCCCGCGACAGCGCCGTTTTTGCGGTACCCGGCGCGGA‑ATTTTCGCGCGCCAGCGCAAGTTTAAACACCCCTCCCCTGTCTGCCg                                          <  1:670465/146‑1 (MQ=255)                                                                                                                      ttGGTGCTGACGATGGTTTTATGGCGGGGCCGGGGTTTAGC‑TTTTTTTGTGGAGAAAGGTTTCCCCG‑CAGGGCCCGCTTTTCGGGAGCCCTCGCGGG‑AATTTTGCGCCCCCGCGGCGGCCTTAAAAACCCTACCCCGTccgccgccaa                                      >  1:55510/1‑144 (MQ=255)                                                                                                                       tGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAAcgc                                   <  1:278410/150‑1 (MQ=255)                                                                                                                         gTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCt                                <  1:585884/151‑1 (MQ=255)                                                                                                                           gCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCtt                               <  1:80593/150‑1 (MQ=255)                                                                                                                                             gCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGgtgt            >  2:1004793/1‑151 (MQ=255)                                                                                                                                              cGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGgtgt            >  2:964771/1‑150 (MQ=255)                                                                                                                                               gggCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTa           >  2:131030/1‑150 (MQ=255)                                                                                                                                                     ggTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAAt      <  1:16092/149‑1 (MQ=255)                                                                                                                                                     ggTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGt    >  1:617647/1‑151 (MQ=255)                                                                                                                                                       tatataGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTg  >  2:119226/1‑151 (MQ=255)                                                                                                                                                       tatataGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTg  >  1:986286/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                          ATCAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTG  >  NC_000913/2252496‑2252796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑