Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,657	0	C	T	43.3%	76.4 / 30.3	60	L320F (CTT→TTT)	yeiI	putative kinase
Reads supporting (aligned to +/- strand):  ref base C (10/24);  new base T (26/0);  total (36/24)
Fisher's exact test for biased strand distribution p-value = 3.87e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGAT  >  NC_000913/2252515‑2252802                                                                                                                                                |                                                                                                                                                    aCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGa                                                                                                                                             <  1:530880/151‑1 (MQ=255) aCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATATC‑tttttttta                                                                                                                                                >  2:1008110/1‑145 (MQ=255)    gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATAGA‑tttttttttgg                                                                                                                                              >  1:715842/1‑142 (MQ=255)     tttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGG‑TTTTTTTTAGGAt                                                                                                                                            >  2:146008/1‑148 (MQ=255)      ttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAGG‑TTTTTTTAAg                                                                                                                                               >  2:672584/1‑144 (MQ=255)       tgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATTTAGG‑TTTTTTTTAGGATaaa                                                                                                                                         >  1:138830/1‑147 (MQ=255)        gtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑Gtt                                                                                                                                     <  1:301260/151‑1 (MQ=255)         tttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAGT‑TTTTTTTTAGGATAAA‑Gttt                                                                                                                                    >  2:278478/1‑151 (MQ=255)          ttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑Gttt                                                                                                                                    <  1:337388/150‑1 (MQ=255)           tATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATTTAACTTTTTTTGAAggtaaaagt                                                                                                                                       >  2:612238/1‑141 (MQ=255)               tGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCGTGGGTTATAAC‑TTTTTTTTAGGGTACaaatt                                                                                                                                     >  2:163539/1‑141 (MQ=255)                gCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATTGTTTTATGGCGGGCCTGGGGTATAAC‑TTTTTTTTAGGATACA‑Gttt                                                                                                                                    >  2:511888/1‑144 (MQ=255)                 cccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACa                                                                                                                            <  2:644508/151‑1 (MQ=255)                      tGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGTTATAAG‑TTTTTTTTAGGAGAAA‑GATTTCGCGGCAGCGcc                                                                                                                       >  1:830712/1‑151 (MQ=255)                       gAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAAG‑TTTTTTTAAGGATAAA‑GGTTTCGCGGCAGCGccc                                                                                                                      >  2:271766/1‑151 (MQ=255)                             gTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTg                                                                                                                <  2:635543/151‑1 (MQ=255)                                   tGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTa                                                                                                           <  2:1045814/150‑1 (MQ=255)                                           aaaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGcgcg                                                                                                  <  1:335197/151‑1 (MQ=255)                                           aaaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAAC‑TTTTTTTGAGGGTACA‑GTTTTCCCGACAGCGCCCCtttt                                                                                                                 >  2:563326/1‑136 (MQ=255)                                            aaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCgg                                                                                                 <  2:677422/151‑1 (MQ=255)                                              ggATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGGTATAGG‑TTTTTTTAAGGGTAAA‑GTTTTCGCGACAGCGCCCGGTTTTCGGTAGGCTGCgggg                                                                                                 >  2:301986/1‑149 (MQ=255)                                                  ggAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AAttt                                                                                          <  1:462365/151‑1 (MQ=255)                                                   gagaACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTc                                                                                         <  1:424938/151‑1 (MQ=255)                                                      aaCAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTcgcg                                                                                      <  1:641172/151‑1 (MQ=255)                                                      aaCAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAAC‑TTTTTTTAAGGATACA‑GTTTTCTCGACAGCGCCCCCTTTTCGGTTGCCTGCGCGGC‑CAttttt                                                                                        >  2:400068/1‑147 (MQ=255)                                                          aTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGG‑TTTTTTTAAGGATAAA‑GTTTCCGCGACAGCGCCCGGTTTTCGGTAGGCTGCGCGGC‑AATTTTGCGCGcc                                                                                  >  2:301038/1‑151 (MQ=255)                                                          aTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAGC‑TTTCTTTTAGGGTAAA‑GTTTTCTCGCCCGCGCCCGGTTTTTGGGAGGCCGCGCGGC‑CATTTCTCGCGcc                                                                                  >  1:268840/1‑151 (MQ=255)                                                           tttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATATA‑TTTTTTTTAAGAGACA‑GATTTCGCCGCAACGCCCCCTTTTCGGGAGGCCGCGCGGCGAAtttgcccccc                                                                                   >  2:704641/1‑143 (MQ=255)                                                            ttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAGC‑TTTTTTTTAGGGGACA‑GTTTTCGCGACAGCGCCCGGTTTTCGGGGGCCTGCGCGGG‑AATTTTtcgcgc                                                                                   >  1:532003/1‑148 (MQ=255)                                                            ttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCa                                                                                 <  1:271698/150‑1 (MQ=255)                                                             tttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGGTATAAG‑TTTTTTTTAGGGAAAA‑GTTTTCCCGAACGCGCCCGGTTTGCGGTAGGCTGGGCGGC‑CATTTTGGGCGcc                                                                                  >  2:681954/1‑148 (MQ=255)                                                               tGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAAC‑TTTCTTTTAGGATACA‑GGTTTCCCGACAACCCCCCCTTTTCGGGTGCCTGCGCCGC‑CATTTTTCGCGcccccgg                                                                             >  2:645441/1‑146 (MQ=255)                                                                gCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGTTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGc                                                                            <  1:442834/151‑1 (MQ=255)                                                                gCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGC‑TTTTTTTTAGGGAAAA‑GATTTCGCGGCAGCGCCCGTTTTTCGGGAGGCTGCGCGGC‑CAATTTGCGCGCCAGCgg                                                                             >  2:908133/1‑150 (MQ=255)                                                                 cTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATAGC‑TTTTTTTTAGGATAAA‑GTTTTCGCGGCAGCGCCCCCTTTTCGGGAGGCCGCGCGGC‑CATTTTCCGCGCCAGCgg                                                                             >  1:382247/1‑149 (MQ=255)                                                                 cTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGTATATAGC‑TTTTTTTTAGGGTAAA‑GTTTTCCCGACAGCGCCCCCTTTTCGGGAGCCCGCGCGGC‑GAATTTGCGCGCCCGCgg                                                                             >  2:567551/1‑149 (MQ=255)                                                                    aCCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAAC‑TTTTTTTTAGGGTACA‑ATTTTCGCGCCCGCGCCCCTTTTTGGGGTGCCTTGGCGGC‑CAtttttcccgcccccgg                                                                             >  1:260730/1‑133 (MQ=255)                                                                      cgcgCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTa                                                                      <  2:979442/151‑1 (MQ=255)                                                                      cgcgCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTa                                                                      <  1:661169/151‑1 (MQ=255)                                                                           cAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGAGATAGG‑TTTTTTGTAGGATAAA‑GATTTCCCCACAGCGGCCCCTTTTCTGGAGCCTGCGCCGC‑AAATTCTCGCGCCACCgggcgccttaa                                                                    >  1:920680/1‑139 (MQ=255)                                                                               gcATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATTTAGA‑TTTTTTTTAGGATAAA‑GTTTTCCCGGCAGCGCCCCGTTTGCGGTAGCCTGCCCGGC‑CATTTTGCGCGCCCGCGGCCGCTTTAAAAaacc                                                              >  1:648829/1‑150 (MQ=255)                                                                                        aGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCt                                                    <  1:248122/151‑1 (MQ=255)                                                                                           aGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGC‑TTTTTTTTAGGATAAA‑GTTTTCGCGACAGCGCCCCCTTTTCGGGAGCCCGGGCGGC‑AATTTTGCGCGCCCGCGGCCGCTTTAAAAACCCCCCCCCCTCTGcc                                                 >  2:430969/1‑151 (MQ=255)                                                                                            gACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAGC‑TTTTTTTTAGGGTACA‑GTTTTCGCGGCAGCGCCCGCTTTTTGGTAGCCCGCGCGGG‑CATTTTTCGCCCCCGCGGCAGCTTTAACAAACCTACCCCCTCTGcc                                                 >  1:810869/1‑150 (MQ=255)                                                                                               aGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGG‑TTTTTTTGAGGATACA‑GTTTTCGCGGCAGCGCCCGGTTTTCGGGAGGCTGCGCGGC‑AATTTTTCGCGCCCGCGGCAGCCTAAAAAACCCCACCCCGTCTGcc                                                 >  2:734139/1‑147 (MQ=255)                                                                                                 ttttGTTGCTGACGTTGTTTTTAGGGCGGGCTTGGTATATAGC‑TTTCTTGAAGGATACA‑TTTCCCGCGACAGCGCCGTTTTTGCGGTACCCGGCGCGGA‑ATTTTCGCGCGCCAGCGCAAGTTTAAACACCCCTCCCCTGTCTGCCg                                                <  1:670465/146‑1 (MQ=255)                                                                                                   ttGGTGCTGACGATGGTTTTATGGCGGGGCCGGGGTTTAGC‑TTTTTTTGTGGAGAAAGGTTTCCCCG‑CAGGGCCCGCTTTTCGGGAGCCCTCGCGGG‑AATTTTGCGCCCCCGCGGCGGCCTTAAAAACCCTACCCCGTccgccgccaa                                            >  1:55510/1‑144 (MQ=255)                                                                                                    tGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAAcgc                                         <  1:278410/150‑1 (MQ=255)                                                                                                      gTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCt                                      <  1:585884/151‑1 (MQ=255)                                                                                                        gCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCtt                                     <  1:80593/150‑1 (MQ=255)                                                                                                                          gCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGgtgt                  >  2:1004793/1‑151 (MQ=255)                                                                                                                           cGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGgtgt                  >  2:964771/1‑150 (MQ=255)                                                                                                                            gggCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTa                 >  2:131030/1‑150 (MQ=255)                                                                                                                                  ggTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAAt            <  1:16092/149‑1 (MQ=255)                                                                                                                                  ggTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGt          >  1:617647/1‑151 (MQ=255)                                                                                                                                    tatataGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTg        >  2:119226/1‑151 (MQ=255)                                                                                                                                    tatataGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTg        >  1:986286/1‑151 (MQ=255)                                                                                                                                     atataGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACATCCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGc       <  1:163478/151‑1 (MQ=255)                                                                                                                                     atataGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTg        <  2:495128/150‑1 (MQ=255)                                                                                                                                      tataGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGcc      >  1:79427/1‑151 (MQ=255)                                                                                                                                       ataGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCg     <  1:734017/151‑1 (MQ=255)                                                                                                                                          gC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGAt  >  2:252701/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                    ACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGC‑TTTCTTGAAGGATACA‑GTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGAT  >  NC_000913/2252515‑2252802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑