Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,263,200	G→A	A154V (GCT→GTT)	tdcD ←	propionate kinase/acetate kinase C, anaerobic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,263,200	0	G	A	100.0%	246.7 / NA	63	A154V (GCT→GTT)	tdcD	propionate kinase/acetate kinase C, anaerobic
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/32);  total (31/32)

GCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTAT  >  NC_000913/3263056‑3263344                                                                                                                                                 |                                                                                                                                                 gcCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCg                                                                                                                                            <  1:650953/151‑1 (MQ=255)  ccACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCgg                                                                                                                                           >  2:607644/1‑151 (MQ=255)  ccACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCgg                                                                                                                                           >  2:719955/1‑151 (MQ=255)   cacaACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGTAGGCCGTATAAATAAACTTCCg                                                                                                                                            <  1:647860/149‑1 (MQ=255)   cacaACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGa                                                                                                                                          <  2:734837/151‑1 (MQ=255)   cacaACCAGGCCGGAGTCATCTTCCACCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCgg                                                                                                                                           >  1:155461/1‑150 (MQ=255)    acaACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAg                                                                                                                                         <  2:967455/151‑1 (MQ=255)          aGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCg                                                                                                                                   <  2:267338/151‑1 (MQ=255)             ccGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCt                                                                                                                                >  1:26147/1‑151 (MQ=255)                gAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCtggt                                                                                                                             >  1:445526/1‑151 (MQ=255)                 aGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCtggtg                                                                                                                            >  1:416395/1‑151 (MQ=255)                  gTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCtggtgg                                                                                                                           <  2:743942/151‑1 (MQ=255)                   tCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCtggtgg                                                                                                                           >  1:28888/1‑150 (MQ=255)                    cATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGa                                                                                                                          <  2:717727/150‑1 (MQ=255)                         tCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAAc                                                                                                                       <  2:25266/148‑1 (MQ=255)                          ccGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGt                                                                                                                   >  2:789594/1‑151 (MQ=255)                              cAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCa                                                                                                               <  1:486284/151‑1 (MQ=255)                                gATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACCTCCGGAGCCATCGTCTGGTGGAAACTGGTATCaaa                                                                                                             <  1:688275/151‑1 (MQ=255)                                    cagcagCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATAcc                                                                                                         >  1:187462/1‑151 (MQ=255)                                     agcagcGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATGTTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCg                                                                                                        >  1:78800/1‑151 (MQ=255)                                     agcagcGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCg                                                                                                        <  2:791947/151‑1 (MQ=255)                                            aaTGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTg                                                                                                 <  2:796657/151‑1 (MQ=255)                                               gtgCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAg                                                                                               <  1:17555/150‑1 (MQ=255)                                               gtgCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGt                                                                                              <  1:68438/151‑1 (MQ=255)                                                  cgcgcTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTAc                                                                                           <  1:226630/151‑1 (MQ=255)                                                   gcgcTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACg                                                                                          <  1:286148/151‑1 (MQ=255)                                                        gggAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGccc                                                                                       <  1:118252/149‑1 (MQ=255)                                                          gAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGaa                                                                                   <  1:295981/151‑1 (MQ=255)                                                          gAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGaa                                                                                   <  2:747275/151‑1 (MQ=255)                                                           aaaCATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCgg                                                                                     >  1:82696/1‑148 (MQ=255)                                                            aaCATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAaat                                                                                 <  1:850373/151‑1 (MQ=255)                                                             aCATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAaata                                                                                <  2:824555/151‑1 (MQ=255)                                                               aTAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAaataat                                                                              >  2:216759/1‑151 (MQ=255)                                                                   cGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATtgct                                                                          <  2:667738/151‑1 (MQ=255)                                                                       gCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTgcg                                                                       <  2:726774/150‑1 (MQ=255)                                                                         gACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCg                                                                    >  1:445131/1‑151 (MQ=255)                                                                                cGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAAt                                                             >  1:528200/1‑151 (MQ=255)                                                                                 gTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATa                                                            <  2:176878/151‑1 (MQ=255)                                                                                       aCCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACtt                                                      >  1:1057268/1‑151 (MQ=255)                                                                                       aCCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACtt                                                      >  1:398722/1‑151 (MQ=255)                                                                                       aCCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACtt                                                      >  2:1015325/1‑151 (MQ=255)                                                                                          aTAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTaa                                                    >  2:389366/1‑150 (MQ=255)                                                                                            aaCGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAAtt                                                 <  2:1015817/151‑1 (MQ=255)                                                                                                gCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCg                                             >  2:469529/1‑151 (MQ=255)                                                                                                 cGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCg                                             >  2:289560/1‑150 (MQ=255)                                                                                                    acacCTAACTCTTCATAATGTTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAAt                                         <  2:78807/151‑1 (MQ=255)                                                                                                    acacCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTaa                                          <  2:386557/150‑1 (MQ=255)                                                                                                          aaCTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGc                                    >  2:318078/1‑150 (MQ=255)                                                                                                               ttCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGt                              >  1:545194/1‑151 (MQ=255)                                                                                                               ttCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGt                              >  2:708589/1‑151 (MQ=255)                                                                                                                tCATAATATTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTg                             >  1:929369/1‑151 (MQ=255)                                                                                                                      tatTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTg                       >  1:303978/1‑151 (MQ=255)                                                                                                                       atTTCCACGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTgg                      >  1:929584/1‑151 (MQ=255)                                                                                                                              cGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAAcg               >  1:254339/1‑151 (MQ=255)                                                                                                                              cGGCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAAc                <  1:399042/150‑1 (MQ=255)                                                                                                                                gCAGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAAcgcg             <  1:953580/151‑1 (MQ=255)                                                                                                                                  aGGCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGAc           >  2:631911/1‑151 (MQ=255)                                                                                                                                   ggCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACg          <  2:28891/151‑1 (MQ=255)                                                                                                                                    gCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACgg         >  2:913623/1‑151 (MQ=255)                                                                                                                                    gCCGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACgg         <  1:982448/151‑1 (MQ=255)                                                                                                                                      cGTATAAATAAACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGat       >  2:296391/1‑151 (MQ=255)                                                                                                                                           aaataaACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGAtattat  <  1:719834/151‑1 (MQ=255)                                                                                                                                           aaataaACTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGAtattat  >  1:463483/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                 GCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTAT  >  NC_000913/3263056‑3263344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑