Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,378,539 | C→G | G38A (GGT→GCT) | rplM ← | 50S ribosomal subunit protein L13 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,378,539 | 0 | C | G | 98.2% | 221.2 / ‑3.3 | 56 | G38A (GGT→GCT) | rplM | 50S ribosomal subunit protein L13 |
Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (31/24); total (32/24) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.14e-01 |
GCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGT > NC_000913/3378392‑3378681 | gCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAgcgc < 1:250843/151‑1 (MQ=255) cTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGcgcg > 1:7920/1‑151 (MQ=255) cTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGcgcg > 2:481583/1‑151 (MQ=255) gTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAgcgcgc > 2:998586/1‑149 (MQ=255) gATACCACCGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGcg < 1:327117/150‑1 (MQ=255) ccaccGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGcgacg > 1:435410/1‑149 (MQ=255) ccaccGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAg < 1:486471/151‑1 (MQ=255) ccGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGcc > 1:687350/1‑150 (MQ=255) aTGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGtt > 1:732969/1‑151 (MQ=255) cGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGc > 2:110868/1‑151 (MQ=255) gtgtGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGcc > 2:966989/1‑150 (MQ=255) tgtgGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGcc > 2:557174/1‑149 (MQ=255) tggtgATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGAc > 1:1055080/1‑151 (MQ=255) tggtgATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGAc > 1:536226/1‑151 (MQ=255) gATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGc < 2:833338/150‑1 (MQ=255) gTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCaga < 1:351436/151‑1 (MQ=255) acacTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGt > 1:1048686/1‑151 (MQ=255) acTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGttt > 2:734907/1‑151 (MQ=255) ttGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTAc > 2:584484/1‑151 (MQ=255) cAGGACTCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCGGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGt < 1:611847/151‑1 (MQ=255) aGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTc > 1:134534/1‑151 (MQ=255) cTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTc > 1:471279/1‑150 (MQ=255) gttgCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTcaac < 2:871871/150‑1 (MQ=255) tgCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTcaacaa < 2:531502/150‑1 (MQ=255) ccGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACAt > 1:1013302/1‑151 (MQ=255) aCAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGt > 2:580159/1‑151 (MQ=255) cAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTc < 2:390674/151‑1 (MQ=255) gCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTcgc < 2:673982/151‑1 (MQ=255) gTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTAc < 1:353082/150‑1 (MQ=255) cAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACgg < 1:27541/150‑1 (MQ=255) cAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACgg < 1:11427/150‑1 (MQ=255) cGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTc > 1:386070/1‑151 (MQ=255) ttCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTg > 2:962968/1‑151 (MQ=255) cAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGt < 1:1059307/151‑1 (MQ=255) gAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGttt > 1:988861/1‑151 (MQ=255) gTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGCGTTTTGCCGGTCGCGTCAGCAACATACCATTCGCTTTTTACGGTTTCTGGTTTAGCTGTaaa < 2:153429/150‑1 (MQ=255) tAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTaaa > 2:58307/1‑149 (MQ=255) tAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTaaa > 2:438064/1‑149 (MQ=255) aaTCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAg < 2:1055255/150‑1 (MQ=255) cACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGtttt > 1:772331/1‑151 (MQ=255) ccGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTc > 1:106297/1‑150 (MQ=255) ccGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTc > 2:718322/1‑150 (MQ=255) cGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCAt < 1:481493/151‑1 (MQ=255) gTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTa < 1:1039599/151‑1 (MQ=255) cGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCtt > 1:788015/1‑151 (MQ=255) cGTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCtt > 2:991353/1‑151 (MQ=255) gTGCGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTa < 2:49505/151‑1 (MQ=255) cGGAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTAccc > 2:873554/1‑151 (MQ=255) ggAGTGTATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTAccc > 1:817312/1‑150 (MQ=255) gtgtATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCaata < 2:573/151‑1 (MQ=255) tgtATTCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCaat < 1:150013/149‑1 (MQ=255) tATTCGGCTGTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCaataa < 1:731749/149‑1 (MQ=255) tCCGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAg < 2:300173/150‑1 (MQ=255) ccGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGtt < 1:184526/151‑1 (MQ=255) ccGCTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGtt < 2:32237/151‑1 (MQ=255) cTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTac > 2:257542/1‑150 (MQ=255) cTTTGTGCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTac > 2:993030/1‑150 (MQ=255) tgtgCTTAGCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGt > 2:125920/1‑151 (MQ=255) | GCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGT > NC_000913/3378392‑3378681 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |