Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	443,040	C→T	intergenic (+43/+11)	thiI → / ← yajL	tRNA s(4)U8 sulfurtransferase/oxidative‑stress‑resistance chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	443,040	0	C	T	100.0%	233.7 / NA	61	intergenic (+43/+11)	thiI/yajL	tRNA s(4)U8 sulfurtransferase/oxidative‑stress‑resistance chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/28);  total (33/28)

GAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTC  >  NC_000913/442892‑443183                                                                                                                                                     |                                                                                                                                                gAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTtgctgc                                                                                                                                               <  1:250481/151‑1 (MQ=255)     aaaaCCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATcc                                                                                                                                           >  2:804182/1‑151 (MQ=255)     aaaaCCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATcc                                                                                                                                           >  2:566068/1‑151 (MQ=255)       aaCCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCg                                                                                                                                          >  1:5860/1‑150 (MQ=255)         ccTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGaca                                                                                                                                       <  1:18227/151‑1 (MQ=255)           tGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACact                                                                                                                                     >  1:1004575/1‑151 (MQ=255)           tGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACact                                                                                                                                     >  2:640635/1‑151 (MQ=255)                 ctgTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGt                                                                                                                               >  2:1049074/1‑151 (MQ=255)                   gtggtgTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCGCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGtaa                                                                                                                             <  2:1029322/151‑1 (MQ=255)                    tggtgTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGtaat                                                                                                                            >  1:672058/1‑151 (MQ=255)                          gAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATtat                                                                                                                      <  2:134838/151‑1 (MQ=255)                          gAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATta                                                                                                                       <  2:804620/150‑1 (MQ=255)                                  ggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCt                                                                                                              <  2:632307/151‑1 (MQ=255)                                  ggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCt                                                                                                              <  1:141708/151‑1 (MQ=255)                                  ggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCt                                                                                                              <  1:447582/151‑1 (MQ=255)                                   gTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTg                                                                                                             <  2:909430/151‑1 (MQ=255)                                     gatgaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTg                                                                                                             <  2:214461/149‑1 (MQ=255)                                       cgtgCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgc                                                                                                         >  1:423744/4‑151 (MQ=255)                                        gaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTTAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgcc                                                                                                        >  2:543963/1‑151 (MQ=255)                                         aGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgcc                                                                                                        >  1:57917/1‑150 (MQ=255)                                                  cAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGtt                                                                                              <  2:420522/151‑1 (MQ=255)                                                  cAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGtt                                                                                              <  1:937522/151‑1 (MQ=255)                                                      cgcTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                                                                                          <  1:879904/151‑1 (MQ=255)                                                       gcTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGAt                                                                                         >  2:62174/1‑151 (MQ=255)                                                          ctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGcc                                                                                      >  2:811864/1‑151 (MQ=255)                                                                gcgcGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACtt                                                                                  <  1:937897/149‑1 (MQ=255)                                                                gcgcGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTc                                                                                 >  2:628573/1‑150 (MQ=255)                                                                    gAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATggg                                                                            >  2:506132/1‑151 (MQ=255)                                                                         gggCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt                                                                       <  2:937919/151‑1 (MQ=255)                                                                              ttAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCAc                                                                   <  2:312185/150‑1 (MQ=255)                                                                                aaCAATGTGCAGGTATATCGCCCGCAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGCCGTTTGCTGCATCAGACACTACTCGTACTAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGcc                                                                >  1:246127/1‑151 (MQ=255)                                                                                 aCAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGccc                                                               >  2:452764/1‑151 (MQ=255)                                                                                   aaTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCcca                                                              >  2:262057/1‑150 (MQ=255)                                                                                        gAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaaccaa                                                         <  2:344539/150‑1 (MQ=255)                                                                                              atatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCg                                                  >  2:723994/1‑151 (MQ=255)                                                                                                atCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGAt                                                <  2:393803/151‑1 (MQ=255)                                                                                                  cGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATa                                               >  1:320862/1‑150 (MQ=255)                                                                                                   gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAt                                             >  1:44436/1‑151 (MQ=255)                                                                                                    cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAt                                             <  1:943180/150‑1 (MQ=255)                                                                                                       gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGCCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTc                                         >  1:690037/1‑151 (MQ=255)                                                                                                                   ttttACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTc                              <  1:819525/150‑1 (MQ=255)                                                                                                                   ttttACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCg                             >  1:927262/1‑151 (MQ=255)                                                                                                                   ttttACGTCGCATATGGTCAGATGCGACGTTTGCTGCGTCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCGCTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTc                              <  1:315643/150‑1 (MQ=255)                                                                                                                    tttACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCg                             <  2:51469/150‑1 (MQ=255)                                                                                                                    tttACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCg                             >  1:381296/1‑150 (MQ=255)                                                                                                                     ttACGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGAt                           >  2:300371/1‑151 (MQ=255)                                                                                                                       aCGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAg                         <  1:521086/151‑1 (MQ=255)                                                                                                                        cGTCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGc                        >  2:877446/1‑151 (MQ=255)                                                                                                                          tCGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTg                      >  1:692532/1‑151 (MQ=255)                                                                                                                           cGCATCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGt                     >  2:560984/1‑151 (MQ=255)                                                                                                                              aTCTGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                  >  1:925156/1‑151 (MQ=255)                                                                                                                              aTCTGGTCAGAAGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCCACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                  >  1:927271/1‑151 (MQ=255)                                                                                                                                 tGGTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCgg               >  2:493748/1‑151 (MQ=255)                                                                                                                                  ggTCAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAccc                 <  2:619519/148‑1 (MQ=255)                                                                                                                                     cAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGcccc           <  2:264924/151‑1 (MQ=255)                                                                                                                                     cAGATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGccc            <  2:966645/150‑1 (MQ=255)                                                                                                                                       gATGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctg         <  2:404255/151‑1 (MQ=255)                                                                                                                                        aTGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctgg        >  1:150734/1‑151 (MQ=255)                                                                                                                                        aTGCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctgg        <  1:137998/151‑1 (MQ=255)                                                                                                                                          gCGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACACGGCCCctgcct      >  1:878501/1‑151 (MQ=255)                                                                                                                                           cGACGTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctggctg     <  1:1048900/151‑1 (MQ=255)                                                                                                                                               gTTTGCTGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTc  >  1:249857/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                GAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTC  >  NC_000913/442892‑443183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑