Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,607,712 C→T intergenic (‑12/‑91) rsmC ← / → holD 16S rRNA m(2)G1207 methyltransferase, SAM‑dependent/DNA polymerase III, psi subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,607,7120CT98.4% 238.1 / ‑3.7 63intergenic (‑12/‑91)rsmC/holD16S rRNA m(2)G1207 methyltransferase, SAM‑dependent/DNA polymerase III, psi subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (24/38);  total (25/38)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

ATGAGCACGGCTGGCCGCGGTATCTAAACGCGCGGGCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTCAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAACTGGGCATTACCCAGTGGTCGCT  >  NC_000913/4607566‑4607858
                                                                                                                                                  |                                                                                                                                                  
aTGAGCACGGCTGGCCGCGGTATCTAAACGCGCGGGCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAAtt                                                                                                                                                <  2:438242/151‑1 (MQ=255)
  gAGCACGGCTGGCCGCGGTATCTAAACGCGCGGGCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTg                                                                                                                                               >  2:864237/1‑150 (MQ=255)
  gAGCACGGCTGGCCGCGGTATCTAAACGCGCGGGCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGa                                                                                                                                              <  1:1002841/151‑1 (MQ=255)
   aGCACGGCTGGCCGCGGTATCTAAACGCGCGGGCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTCGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGAt                                                                                                                                             <  2:558634/151‑1 (MQ=255)
            ggCCGCGGTATCTAAACGCGCGGGCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTggcggc                                                                                                                                    <  1:746049/151‑1 (MQ=255)
                gcgGTATCTAAACGCGCGGGCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGAtt                                                                                                                                <  2:797273/151‑1 (MQ=255)
                gcgGTATCTAAACGCGCGGGCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGAtt                                                                                                                                >  2:994581/1‑151 (MQ=255)
                 cgGTATCTAAACGCGCGGGCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGAtt                                                                                                                                >  2:952258/1‑150 (MQ=255)
                 cgGTATCTAAACGCGCGGGCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATta                                                                                                                               <  2:289654/151‑1 (MQ=255)
                     aTCTAAACGCGCGGGCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTCAATTGATACTGGCGGCGATTATAGc                                                                                                                           >  1:348800/1‑151 (MQ=255)
                           aCGCGCGGGCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATg                                                                                                                     >  2:468055/1‑151 (MQ=255)
                             gctcgGGAAGGTCATCCTGTAAGTCACCGGCAAACAGAATACGGCTTTGGTCGAAAGCATCACTGTGACGCAGCAAGACTTCACTTGCGGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGtt                                                                                                                   <  1:90049/148‑1 (MQ=255)
                                cgGGCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGgc                                                                                                                <  2:698081/151‑1 (MQ=255)
                                 gggCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGgcg                                                                                                               >  1:362896/1‑151 (MQ=255)
                                 gggCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGgcg                                                                                                               <  2:721504/151‑1 (MQ=255)
                                 gggCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGgcg                                                                                                               <  1:662055/151‑1 (MQ=255)
                                       gTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTAt                                                                                                         <  2:558780/151‑1 (MQ=255)
                                          aTCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATcga                                                                                                      <  1:607920/151‑1 (MQ=255)
                                               gTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAAt                                                                                                 <  2:789682/151‑1 (MQ=255)
                                                 aaGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAAttt                                                                                               >  1:184988/1‑151 (MQ=255)
                                                   gTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCACCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCGCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAAt                                                                                                 >  1:253994/1‑147 (MQ=255)
                                                       ccGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCtat                                                                                          >  2:804150/1‑150 (MQ=255)
                                                       ccGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCta                                                                                           <  1:258659/149‑1 (MQ=255)
                                                          gCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATAttt                                                                                      <  1:632780/151‑1 (MQ=255)
                                                          gCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATAttt                                                                                      >  2:65098/1‑151 (MQ=255)
                                                          gCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATAttt                                                                                      <  1:452692/151‑1 (MQ=255)
                                                            aaaCAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTgc                                                                                    >  2:747453/1‑151 (MQ=255)
                                                            aaaCAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTgc                                                                                    <  1:188939/151‑1 (MQ=255)
                                                            aaaCAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTgc                                                                                    <  1:197516/151‑1 (MQ=255)
                                                            aaaCAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTgc                                                                                    <  1:858419/151‑1 (MQ=255)
                                                              aCAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTgcgc                                                                                  <  1:944085/151‑1 (MQ=255)
                                                               cAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGcc                                                                                 >  1:917564/1‑151 (MQ=255)
                                                                  aaTACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCt                                                                              >  1:872528/1‑151 (MQ=255)
                                                                     aCGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGac                                                                           <  1:117046/151‑1 (MQ=255)
                                                                      cGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGaca                                                                          <  1:477947/151‑1 (MQ=255)
                                                                       ggCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGaca                                                                          >  1:768642/1‑150 (MQ=255)
                                                                             gTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGa                                                                    <  1:517706/150‑1 (MQ=255)
                                                                              ttCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGc                                                                  >  2:632025/1‑151 (MQ=255)
                                                                                cGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCg                                                                 <  1:721801/150‑1 (MQ=255)
                                                                                         caCTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTAt                                                        <  1:288017/150‑1 (MQ=255)
                                                                                             gtgACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACa                                                    <  2:470207/150‑1 (MQ=255)
                                                                                                    agcaAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCcgacg                                            >  2:321039/1‑151 (MQ=255)
                                                                                                          aCTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTg                                      >  1:671617/1‑151 (MQ=255)
                                                                                                                 ttGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGtt                                >  1:480237/1‑150 (MQ=255)
                                                                                                                  tGCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTAc                              >  2:607341/1‑151 (MQ=255)
                                                                                                                   gCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTAc                              <  2:335213/150‑1 (MQ=255)
                                                                                                                   gCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTAc                              >  2:176792/1‑150 (MQ=255)
                                                                                                                   gCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACa                             <  1:109483/151‑1 (MQ=255)
                                                                                                                   gCCGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACa                             <  1:496469/151‑1 (MQ=255)
                                                                                                                     cGGGGTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGc                           >  2:966457/1‑151 (MQ=255)
                                                                                                                      ggggTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGc                           >  2:1003281/1‑150 (MQ=255)
                                                                                                                        ggTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAAc                        <  1:685089/151‑1 (MQ=255)
                                                                                                                        ggTAAATGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAAc                        <  1:61261/151‑1 (MQ=255)
                                                                                                                            aaTGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAACTggg                    >  2:308981/1‑151 (MQ=255)
                                                                                                                            aaTGCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAACTggg                    <  1:954183/151‑1 (MQ=255)
                                                                                                                               gCAGACATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAACTGGGCAt                 >  2:254904/1‑151 (MQ=255)
                                                                                                                                   aCATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAACTGGGCATTAcc             >  2:948154/1‑151 (MQ=255)
                                                                                                                                    cATGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAACTGGGCATTAccc            <  2:377841/151‑1 (MQ=255)
                                                                                                                                     aTGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAACTGGGCATTACCCa           <  2:164285/151‑1 (MQ=255)
                                                                                                                                     aTGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAACTGGGCATTACCCa           <  1:952098/151‑1 (MQ=255)
                                                                                                                                     aTGGAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAACTGGGCATTACCCa           <  1:864089/151‑1 (MQ=255)
                                                                                                                                       ggAATGCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAACTGGGCATTACCCAg          <  1:323848/150‑1 (MQ=255)
                                                                                                                                            gatccttAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACCACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTTCAGCAACTTGGCATTACCCAGGGGTc     >  1:493523/3‑150 (MQ=255)
                                                                                                                                            gCTCCTTAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAACTGGGCATTACCCAGTGGTc     <  1:932796/150‑1 (MQ=255)
                                                                                                                                              tccttAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAACTGGGCATTACCCAGTGGTCGc   >  1:901688/1‑150 (MQ=255)
                                                                                                                                               ccttAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAACTGGGCATTACCCAGTGGTCGCt  <  2:173027/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATGAGCACGGCTGGCCGCGGTATCTAAACGCGCGGGCAGGTCATCCTGTAAGTCTCCGGCAAACAGAATACGGCTTTGTTCGAAATCATCACTGTGACGCAGCAAGACTTCACTTGCCGGGGTAAATGCAGACATGGAATGCTCCTCAATTGATACTGGCGGCGATTATAGCCATATGTTGGCGCGGTATCGACGAATTTGCTATATTTGCGCCCCTGACAACAGGAGCGATTCGCTATGACATCCCGACGAGACTGGCAGTTACAGCAACTGGGCATTACCCAGTGGTCGCT  >  NC_000913/4607566‑4607858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑