Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	874,067	G→A	R363R (CGG→CGA)	yliE →	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	874,067	0	G	A	100.0%	336.3 / NA	84	R363R (CGG→CGA)	yliE	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/41);  total (43/41)

CTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGGTCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACTGTGTGGTCTGGAAGCCGTGGAAGCGGTGTTTGG  >  NC_000913/873919‑874213                                                                                                                                                     |                                                                                                                                                   cTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATc                                                                                                                                                  >  1:1042981/1‑151 (MQ=255) cTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATc                                                                                                                                                  >  1:763563/1‑151 (MQ=255)   gAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCaa                                                                                                                                                <  1:680627/151‑1 (MQ=255)   gAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCa                                                                                                                                                 >  1:269256/1‑150 (MQ=255)    aaaTGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCaaa                                                                                                                                               <  1:912105/151‑1 (MQ=255)      aTGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCaaata                                                                                                                                             <  1:866767/151‑1 (MQ=255)       tGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCaaata                                                                                                                                             >  2:513619/1‑150 (MQ=255)        gACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCaaataaa                                                                                                                                           <  2:392903/151‑1 (MQ=255)        gACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCaaataa                                                                                                                                            <  1:531090/150‑1 (MQ=255)        gACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCaaataa                                                                                                                                            >  2:142433/1‑150 (MQ=255)           agagCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTg                                                                                                                                        >  2:967597/1‑151 (MQ=255)            gagCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGc                                                                                                                                       <  2:323244/151‑1 (MQ=255)              gCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGctc                                                                                                                                     >  1:474483/1‑151 (MQ=255)                 ggTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGctct                                                                                                                                    <  1:721151/149‑1 (MQ=255)                 ggTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCa                                                                                                                                  <  2:269837/151‑1 (MQ=255)                  gTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCAc                                                                                                                                 >  2:561282/1‑151 (MQ=255)                          gCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGAtct                                                                                                                           >  1:1053433/1‑149 (MQ=255)                            tGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCtt                                                                                                                        >  1:990459/1‑150 (MQ=255)                            tGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCtt                                                                                                                        >  1:937815/1‑150 (MQ=255)                             ggCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCtt                                                                                                                        <  1:30073/149‑1 (MQ=255)                                gTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGAt                                                                                                                   <  2:775192/151‑1 (MQ=255)                                    ttGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAg                                                                                                                <  1:1067869/150‑1 (MQ=255)                                     tGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAgg                                                                                                               <  2:433583/150‑1 (MQ=255)                                     tGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAgg                                                                                                               >  1:993629/1‑150 (MQ=255)                                     tGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAgg                                                                                                               >  2:590446/1‑150 (MQ=255)                                     tGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAgg                                                                                                               >  2:993336/1‑150 (MQ=255)                                       ccTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGaaa                                                                                                            <  2:488175/151‑1 (MQ=255)                                       ccTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGaaa                                                                                                            <  2:432626/151‑1 (MQ=255)                                       ccTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGaaa                                                                                                            <  1:508224/151‑1 (MQ=255)                                        cTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGaa                                                                                                             >  1:612281/1‑149 (MQ=255)                                          gACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAg                                                                                                           <  1:124505/149‑1 (MQ=255)                                          gACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTg                                                                                                         <  2:134491/151‑1 (MQ=255)                                            cTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGt                                                                                                       >  2:474361/1‑151 (MQ=255)                                                   gATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTttatta                                                                                                <  1:501933/151‑1 (MQ=255)                                                   gATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTttatt                                                                                                 >  2:765022/1‑150 (MQ=255)                                                      cAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTaaa                                                                                              >  2:537074/1‑150 (MQ=255)                                                       aaaTCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTaaa                                                                                              >  1:481208/1‑149 (MQ=255)                                                         aTCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAat                                                                                           >  1:1005172/1‑150 (MQ=255)                                                             aGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGc                                                                                      >  1:407010/1‑151 (MQ=255)                                                              ggCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCg                                                                                     >  1:149383/1‑151 (MQ=255)                                                                  ctGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGatt                                                                                  >  2:272801/1‑150 (MQ=255)                                                                    gAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGattatt                                                                               >  2:544278/1‑151 (MQ=255)                                                                     aaaaTTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGattatt                                                                               >  1:880387/1‑150 (MQ=255)                                                                      aaaTTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTg                                                                              >  1:1014462/1‑150 (MQ=255)                                                                      aaaTTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGa                                                                             <  2:443487/151‑1 (MQ=255)                                                                       aaTTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTg                                                                              >  2:105846/1‑149 (MQ=255)                                                                       aaTTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAg                                                                            <  1:286856/151‑1 (MQ=255)                                                                          tCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCt                                                                         >  2:528061/1‑151 (MQ=255)                                                                           cGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCt                                                                         >  2:444887/1‑150 (MQ=255)                                                                            gTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTa                                                                        >  1:822810/1‑150 (MQ=255)                                                                                 ggCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACaata                                                                  <  1:707228/151‑1 (MQ=255)                                                                                   cGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACaataat                                                                <  1:637878/151‑1 (MQ=255)                                                                                    gATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACaataat                                                                <  1:785189/150‑1 (MQ=255)                                                                                     aTGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCa                                                              <  2:319870/151‑1 (MQ=255)                                                                                       gCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGt                                                            <  1:590344/151‑1 (MQ=255)                                                                                       gCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGt                                                            >  2:1052548/1‑151 (MQ=255)                                                                                        cGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTa                                                           <  1:1046473/151‑1 (MQ=255)                                                                                         gAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTAt                                                          >  1:755336/1‑151 (MQ=255)                                                                                           aaGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCt                                                        <  2:697408/151‑1 (MQ=255)                                                                                             gagaTTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTgg                                                      <  2:1043154/151‑1 (MQ=255)                                                                                                  tATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCtgtg                                                 >  1:30410/1‑151 (MQ=255)                                                                                                   aTCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTgg                                                <  1:576733/151‑1 (MQ=255)                                                                                                       gCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGat                                            <  2:990621/151‑1 (MQ=255)                                                                                                        cATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGatc                                           >  1:71297/1‑151 (MQ=255)                                                                                                        cATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGatc                                           <  2:269324/151‑1 (MQ=255)                                                                                                        cATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGatc                                           <  2:386447/151‑1 (MQ=255)                                                                                                           cAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGTTGTGGGGatc                                           >  1:803845/1‑148 (MQ=255)                                                                                                                 ttCGATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACtg                                  >  1:335106/1‑151 (MQ=255)                                                                                                                    gATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACtgtgt                               >  2:516498/1‑151 (MQ=255)                                                                                                                    gATCGCGGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACtgtgt                               >  2:138219/1‑151 (MQ=255)                                                                                                                        gcgGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACTGTGTGGTc                           >  2:86104/1‑151 (MQ=255)                                                                                                                        gcgGAAATGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACTGTGTGGTc                           <  2:296377/151‑1 (MQ=255)                                                                                                                            aaaTGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACTGTGTGGTCTGGa                       <  2:612384/151‑1 (MQ=255)                                                                                                                              aTGAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACTGTGTGGTCTGGa                       >  1:24152/1‑149 (MQ=255)                                                                                                                                gAAAGATGCCGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACTGTGTGGTCTGGAAGcc                   <  2:728281/151‑1 (MQ=255)                                                                                                                                        ccGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACTGTGTGGTCTGGAAGCCGTGGAAGc           <  1:139651/151‑1 (MQ=255)                                                                                                                                        ccGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACTGTGTGGTCTGGAAGCCGTGGAAGc           <  2:149440/151‑1 (MQ=255)                                                                                                                                        ccGAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATATGGCTGTGGGGATCATCAAACTGTGTGGTCTGGAAGCCGTGGAAGc           >  1:179972/1‑151 (MQ=255)                                                                                                                                          ttaaTTGAAAGATCAACTAAATTGCTCTCACTGATCTTTTACGATAAGGAACGTGCTTCTATTAAAAATATCGCGATTATTCCGTCTTACAATAATCAGTATCTGGCTGATGGGTTCATCACACTGTGACGTCTGGAGATCGTGGa              >  1:81339/3‑146 (MQ=255)                                                                                                                                          gAAATTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACTGTGTGGTCTGGAAGCCGTGGAAGCgg         >  1:5610/1‑151 (MQ=255)                                                                                                                                            aaTTGAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACTGTGTGGTCTGGAAGCCGTGGAAGCGgtg       <  1:513524/151‑1 (MQ=255)                                                                                                                                                gAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACTGTGTGGTCTGGAAGCCGTGGAAGCGGTGttt    <  2:777512/150‑1 (MQ=255)                                                                                                                                                gAACGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACTGTGTGGTCTGGAAGCCGTGGAAGCGGTGttt    <  1:561182/150‑1 (MQ=255)                                                                                                                                                 aaCGATCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACTGTGTGGTCTGGAAGCCGTGGAAGCGGTGTTTgg  <  2:492153/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAAGAGATTATCAGCATCAAAAATTCGATCGCGGAAATGAAAGATGCCGAAATTGAACGGTCAAATAAATTGCTCTCACTGATCTCTTACGATCAGGAAAGTGGTTTTATTAAAAATATGGCGATTATTGAGTCTAACAATAATCAGTATCTGGCTGTGGGGATCATCAAACTGTGTGGTCTGGAAGCCGTGGAAGCGGTGTTTGG  >  NC_000913/873919‑874213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑